Methods for non-invasive prenatal ploidy calling
An individual, targeted technique for noninvasive prenatal ploidy calling
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[0316] Amplification of DNA, a process that converts a small amount of genetic material into a larger amount of genetic material containing a similar set of genetic data, can be performed by a variety of methods including but not limited to polymerase chain reaction (PCR), ligand-mediated Guided PCR, degrading oligonucleotide-primed PCR, multiple displacement amplification, allele-specific amplification techniques, molecular inversion probes (MIPs), padlock probes, other circular probes, and combinations thereof. Many variations of standard protocols can be used, such as increasing or decreasing the time of certain steps in the protocol, increasing or decreasing the temperature of certain steps, increasing or decreasing the amounts of various reagents, etc. DNA amplification converts an initial sample of DNA into a sample of similar but greater amounts of DNA in the sequence set. In some cases, amplification may not be required.
[0317] The genetic data of an individual of i...
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