The invention belongs to the technical field of the
quality safety detection of agricultural products, and relates to the detection of AFB1 (
Aflatoxin B1) in the agricultural products, in particular to an
electrochemical detection method for the AFB1 based on a strand displacement amplification and surface proximity
hybridization reaction and application thereof. A
hybrid complex formed by a
biotin-labelled AFB1
aptamer and a Block probe is prefixed on a surface of a
magnetic bead; in the presence of a target, the AFB1 and the Block probe are combined with the
aptamer in a competitive manner; the replaced Block probe can trigger a strand displacement amplification reaction so as to produce a lot of amplicons; and the amplicons can cooperatively participate in the surface proximity
hybridization reaction on a surface of a screen-printed
electrode, so as to introduce an electroactive probe to generate a corresponding electrical
signal. Compared with specific experiments and conventional ELISA (
Enzyme-linked Immuno
Sorbent Assay) methods, the
electrochemical detection method for the AFB1 disclosed by the invention has the advantages of good specificity, high sensitivity, reliability and accuracy, and is expected to provide a technical selection for large-scale screening of the AFB1 in agricultural products including corn.