The invention relates to a method for detecting embryo chromosome abnormality by utilizing blastula culture solution. By detecting free DNAs of an embryo source through embryo early stage in vitro solution, namely the blastula culture solution, trace DNAs undergo uniform whole genome amplification, a method of next generation sequencing and the like are utilized to analyze amplified DNA products, so that the chromosome condition of embryos, namely whether integral or local aneuploid of the chromosome arises, is judged. The blastula culture solution, which is waste at the embryo in vitro culture stage in the in vitro fertilization operation process, serves as a detection sample. According to the non-invasive method, cell damage to the embryos during conventional blastomere biopsy or blastula trophoblast cell biopsy sampling is avoided, no additional trouble is caused clinically, the operation during sample obtaining is simplified, and the safe reliability and simplicity are higher.