The invention provides a method for detecting
chromosome abnormality of embryos by the aid of
blastocyst cultivation solution without
zona pellucida. The method which is particularly an in-vitro non-therapeutic method for detecting the
chromosome abnormality of the embryos by the aid of the
blastocyst cultivation solution includes steps of (a), providing the cultivation solution from
blastocyst cultivation systems; (b), carrying out
gene detection on the cultivation solution so as to authenticate whether chromosomes of the embryos are abnormal or not. The
zona pellucida of the embryos in the blastocyst cultivation systems is removed before the embryos are cultivated, the embryos are cultivated in the blastocyst systems for 3-6 days, and preferably, the cultivation solution is separated from the cultivation systems after 4 days. The method has the advantages that
contamination and interference risks due to redundant sperms and parent-source granular cells in IVF (in-vitro fertilization) and ICSI (intracytoplasmic
sperm injection) technical procedures can be eliminated, and whether the chromosomes of the embryos are abnormal or not can be accurately authenticated.