201 results about "Human chorionic gonadotropin" patented technology

The invention discloses an integral detecting reacting board and protein chip agent box of Carcinoembryonic antigen,CEA, 15-3 CA15-3(Carbohydrate Antigen 15-3,CA15-3), CA 125 (Cancer antigen 125,CA125), Squamous cell carcinoma antigen,SCC, Human papillomavirus antibody,HPVAb, beta-human chorionic gonadotropin, beta-HCG and alpha-fetoprotein,AFP, wherein the reacting hole board contains base and reacting hole on the base; the reacting hole contains 2-384 sample holes, 2-200 standard sample holes; the fixing phase carrier is set on the bottom of each reacting hole, which is covered by seven antigen or antibody molecule arrays with anti-CEA, anti-CA15-3,anti-CA125,anti-SCC,HPV,anti- beta-HCG,anti-AFP.

The present invention relates to peptides of one or more portions of the human chorionic gonadotropin beta-chain as well as methods of promoting hematopoiesis, using human chorionic gonadotropin, employing the beta-chain of human chorionic gonadotropin, peptides containing a sequence of one or more portions of the beta-chain of human chorionic gonadotropin and derivatives and analogues thereof. The invention further relates to fractions of sources and or perparations of human chorionic gonadotropin, such as fractions of humna early pregnancy urine, which fractions have pro-hematopoietic activity. The present invention further relates to pharmaceutical compositions for promoting hematopoiesis.

The invention relates to a loach fry artificial breeding method which is characterized by including the steps of selecting healthy parental loaches above two winters old and conducting enrichment culture for 25-35 days; then conducting hybrid injection of HCG (human chorionic gonadotropin) and DOM (domperidone) for artificially hastening parturition; after 10-14 hours after parturition hastening, collecting fertilized eggs; and then conducting artificial incubation and larva breeding, thus obtaining the loach fry. In the invention, through the enrichment culture of the parental loaches, synchronous maturing is promoted and oogonium percentage is enhanced; with the parental loach parturition hastening technology, synchronous egg laying can be realized; with the hybrid injection method, the ovarian development and the egg laying effect of the loaches can be promoted, so that the synchronous egg laying rate of the parental loaches achieves 40%; and an inflation incubation way is adopted on the fertilized eggs, so that the incubation rate of the fertilized eggs can be greatly improved. Through enrichment culture, the survival rate of the loach fry can be enhanced remarkably.

A method for synchronizing ovulation in sows and gilts by a single injection of hormones is disclosed. A hormone, gonadotropin releasing hormone (GnRH), luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), analogues, derivatives, agonists or combinations thereof is administered to an open sow post weaning at a specific time to stimulate ovulation of mature responsive follicles. The sow is then bred, without heat detection, at a specific subsequent timed interval after injection with hormone, with one or two artificial or natural breedings. In gilts, the hormone is injected at a timed interval from onset of estrus or at a specific timed interval following Prostaglandin F2a for those gilts which have been held in a state of pseudopregnancy.

The present invention relates to a fusion protein in which a carboxy terminal of human erythropoietin (EPO) is fused with a carboxy terminal peptide fragment of β subunit of human chorionic gonadotropin (HCG), to DNA encoding the fusion protein, and to a method for preparation of the fusion protein. The fusion protein has the enhanced in vivo activity of erythropoietin.

The present invention relates to a fusion protein in which a carboxy terminal of human erythropoietin (EPO) is fused with a carboxy terminal peptide fragment of β subunit of human chorionic gonadotropin (HCG), to DNA encoding the fusion protein, and to a method for preparation of the fusion protein. The fusion protein has the enhanced in vivo activity of erythropoietin.

Methods and kits are provided for diagnosing, monitoring, or predicting the conditions of pre-eclaimpsia, fetal chromosomal aneuploidy, and pre-term labor in a pregnant woman, as well as for detecting pregnancy in a woman, by quantitatively measuring in the maternal blood the amount of one or more mRNA species encoding human chorionic gonadotropin β subunit (hCG-β), human placental lactogen (hPL), human corticotropin releasing hormone (hCRH), KiSS-1 metastasis-suppressor (KISS1), tissue factor pathway inhibitor 2 (TPFI2), placenta-specific 1 (PLAC1), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and comparing the amount of the mRNA species with a standard control.

The invention discloses a breeding method of the loach, which can be used for improving the spawning expediting rate and the fertilization rate of the loach and the livability of the loach seedlings. The breeding method of the load comprises the steps of: a. respectively breeding the male loach and the female loach a half month before spawning expediting; b. adding a biological odinagogue into forage one week before spawning expediting, wherein the biological odinagogue specifically comprises 15-20g of Chinese angelica, ligusticum wallichii, folium artemisiae argyi, andrographis paniculata, rehmanniae vaporata and compound vitamins respectively, and the adding quantity is that 5-10g of the biological odinagogue is added into each kilogram of the forage, and continuously feeding the forage which is added with the biological odinagogue for 7 days; and c. making sets according to the proportion that the ratio of the female loach to the male loach is 1:1.5, and injecting 100-150 international units of HCG (human chorionic gonadotropin) into each loach. The breeding method has the beneficial effects that the female loach and the male loach are synchronously estrous after the breeding method is used, so that the spawning expediting rate and the fertilization rate of the loach are greatly improved. After a preferable mode of the breeding method is adopted, the cost can be reduced when the seedlings are bred, and the bait with an enough quantity can be timely provided, so that the livability of the loach seedling is guaranteed, and the large-scale production is realized.

The invention discloses a method and application for inducing human umbilical cord mesenchyme stem cells to be differentiated into testicular interstitial cells. The method comprises the following step of culturing human umbilical cord mesenchyme stem cells of patients suffering from adenovirus and carrying mice steroidogenic factor-1 genes in a DMEM-F12 culture solution containing 0.3-3ng / ml of luteinizing hormone, 200-800mu M of dibutyryl cyclic adenosine monophosphate, 5*10<-6>-5*10<-4>M of all-trans retinoic acid (ATRA), 10mU / ml of human chorionic gonadotropin and 2.4uM of adrenocorticotrophic hormone for a week. Induced by the method in the invention, the human umbilical cord mesenchyme stem cells can be differentiated into testicular interstitial cells in vitro and provides important sources of cells for treating testosterone shortage by the cell replacing method or the genetic method.

The present invention provides for a method for stimulating the proliferation of pluripotential stem cells in a mammal comprising administration of pregnancy related compounds more particularly human chorionic gonadotropin, leutenizing hormone or prolactin. The present invention further provides for a method of treatment of tissues or organs experiencing cellular damage, injury or disease.

Provided is a fusion protein comprising, at its carboxy terminal of human erythropoietin (EPO), a mutant having one to four amino acid substitutions in the carboxy terminal peptide (CTP) fragment of a human chorionic gonadotropin (HCG) β subunit, for increasing an in vivo half-life activity of EPO. The in vivo half-life can be greatly elongated while retaining the intrinsic activity of the EPO, without increasing the sugar chain content.

The invention discloses a quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for beta human chorionic gonadotropin (beta-hCG). The kit comprises beta-hCG calibrators, magnetic particle suspension coupled with streptavidin, a beta-hCG antibody labeled with biotin, a beta-hCG abzyme combination, a beta-hCG quality controller, chemiluminescence liquor A, chemiluminescence liquor B, 20 times concentrated washing liquor, and a reaction tube, wherein enzyme adopted by the beta-hCG abzyme combination is horse radish peroxidase with the purity RZ being more than or equal to 3.0 and the activity being more than or equal to 250U / ml. The invention also discloses a preparation method of the kit. Compared with the conventional kit, the quantitative detection kit is simple and convenient to operate, is safe, does not cause environment pollution, and also has the advantages of wide concentration range, low cost, good stability and the like of detection samples.

The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

The invention discloses hyperglycosylated Extendin-4, fusion protein of analogue thereof, and a preparation method and the application of fusion protein. The fusion protein comprises Extendin-4, the analogue of the Extendin-4, a flexible peptide joint, at least one human chorionic gonadotropin beta carboxyl terminal peptide rigid unit and a human immunoglobulin Fc fragment. The invention also discloses the preparation method and the application of the fusion protein. The fusion protein has optimal biological activity, obviously prolonged circulation half-time, lowered immunogenicity and improved bioavailability. The fusion protein can be used for treating diabetes, obesity and other diseases benefited by lowering fasting plasma glucose, inhibiting stomach and / or bowel movement and inhibiting and / or bowel evacuation or inhibiting food intake.

A method for synchronizing ovulation in sows and gilts by a single injection of hormones is disclosed. A hormone, gonadotropin releasing hormone (GnRH), luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), analogues, derivatives, agonists or combinations thereof is administered to an open sow post weaning at a specific time to stimulate ovulation of mature responsive follicles. The sow is then bred, without heat detection, at a specific subsequent timed interval after injection with hormone, with one or two artificial or natural breedings. In gilts, the hormone is injected at a timed interval from onset of estrus or at a specific timed interval following Prostaglandin F2a for those gilts which have been held in a state of pseudopregnancy.

The invention relates to a test strip and a method for fast quantitative detection of human chorionic gonadotropin (HCG). The test strip comprises a sample loading pad, a marker pad, an NC membrane, a sample suction pad and a plastic plate. The sample loading pad, the marker pad, the NC membrane and the sample suction pad are orderly adhered to the plastic plate. The marker pad is coated with colloidal gold-marked mouse anti-human HCG-beta monoclonal antibodies. The NC membrane is coated with a detection line T composed of mouse anti-human HCG-alpha antibodies and a quality control line C composed of goat anti-mouse IgG antibodies. On the NC membrane, a detection line T side close to the marker pad is provided with a sample loading indication line for indicating a sample loading position. Through loading of a sample on the NC membrane, the test strip can solve the problem that the existing detection reagent produces a hook effect because of too high HCG content, and can realize quantitative detection of HCG in blood or urine by a matched immunochromatographic assay interpretoscope.

A method for synchronizing ovulation in sows and gilts by a single injection of hormones is disclosed. A hormone, gonadotropin releasing hormone (GnRH), luteinizing hormone (LH), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), analogues, derivatives, agonists or combinations thereof is administered to an open sow post weaning at a specific time to stimulate ovulation of mature responsive follicles. The sow is then bred, without heat detection, at a specific subsequent timed interval after injection with hormone, with one or two artificial or natural breedings. In gilts, the hormone is injected at a timed interval from onset of estrus or at a specific timed interval following Prostaglandin F2a for those gilts which have been held in a state of pseudopregnancy.

The invention discloses a human chorionic gonadotropin (hCG) cycle detection test kit, which comprises an upper cap and a cassette, wherein two parallel grooves are arranged in the cassette, a piece of T1 detection test paper is arranged in one of the grooves, and a piece of T2 detection test paper is arranged in the other groove. The preparation method of the test kit comprises the following steps: preparing a nitrocellulose membrane; labeling a colloidal gold pad and drying; and assembling and cutting. The test kit disclosed by the invention adopts the principle of double antibody sandwich and immunochromatographic assay to qualitatively detect hCG in urine of pregnant women, the detection procedure is simple, the result can be observed within 5 min, a user can self-detect, and the detection result is accurate. Based on the color of a first test line and a second test line, a user can estimate gestational age and pregnancy time.

The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG is a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

The invention relates to a novel synthesis method for a multilayer protection hyperstable water-soluble single fluorescent quantum dot and a fluorescent microsphere. The method comprises the following steps: transferring an oil-soluble fluorescent quantum dot to a water phase by using an amphoteric oligomer; then wrapping a water-soluble single fluorescent quantum dot and a fluorescent microsphere in silica; allowing the surfaces of the single fluorescent quantum dot and the fluorescent microsphere wrapped by silica to carry hydrophobic groups; and finally preparing the multilayer protection hyperstable water-soluble single fluorescent quantum dot and the fluorescent microsphere by using the amphoteric oligomer again. With the method, the fluorescent quantum dot can be protected from a destructive effect of an external environment (e.g., a strong acid, a strong alkali, a salt, etc.) to a greatest extent. The multilayer protection hyperstable water-soluble single fluorescent quantum dot and the fluorescent microsphere can be used in a microporous immunostrip biological detection system for detection of human chorionic gonadotropin (HCG), human immunodeficiency virus (HIV), hepatitis and clenbuterol--ractopamine(Rac) residual and the like in swine urine.

The invention discloses an artificial propagation method for Taiwan mud fishes and belongs to the propagation technologies for fresh water mud fishes. The method includes the following steps that (1), parent mud fish fries are selected; (2), artificial spawning induction is performed; (3), artificial fertilization is performed; (4), hatching is performed. According to the technology, the method that human chorionic gonadotropin (HCG), hypophyses or luteotropin releasing hormone d-ala analogs and other spawning induction medicine are injected to the backs of the parent mud fishes is adopted, so that the spawning induction rate of the parent mud fishes is increased over 91.0%; according to the technology, the fertilization rate of the parent mud fishes is increased over 96.6% through artificial fertilization, and the hatching rate of the parent mud fishes is increased over 98.0% through still water hatching; the survival rate of the parent mud fishes after artificial propagation reaches 90.9%. Propagation is performed by the utilization of the method, so that the survival rate, the spawning induction rate, the fertilization rate and the hatching rate of the Taiwan mud fishes are much higher than the corresponding rates through natural propagation and traditional propagation method for the mud fishes, industrialized production of the Taiwan mud fishes can be achieved, the fries are provided for large-scale farming, and high economical benefits are obtained.

The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

The invention relates to an artificial eel propagation method, which includes: a, ripening parent fishes, breeding catadromous parent reels for seven days, gradually increasing salinity of water, and subjecting the parent eels to back intramuscular injection of ripening agent-carp pituitary gland homogenate and HCG (human chorionic gonadotropin); b, performing induced spawning of the parent eels, subjecting female eels to injection of oxytocin 17 alpha-hydroxyl progesterone and HCG, subjecting sperm-producing male eels to injection of mixture of CPE (carp pituitary extract) with HCG, collecting naturally spawned fertilized eggs on the next day, and subjecting the female eels laying no eggs to semi-dry artificial insemination; and c, hatching the fertilized eggs, immediately transferring the fertilized eggs to a special-made hatching barrel for hatching, and slightly aerating, changing half of water every four hours until fry are hatched successfully. The artificial eel propagation method has the advantages that anesthetic loss and operational injury can be prevented, injection dosage of hormones is reduced, a great amount of eels can mate and lay eggs naturally under artificial induced spawning, artificial insemination can be complete securely in a planned way, level of high-quality fertilized eggs and alevin of eels artificially propagated is increased.

The invention discloses an artificial propagation method of esox reichertii, belongs to the cultivation filed of the esox reichertii, and is mainly used for solving the technical problems that the existing natural egg-laying process of the wild esox reichertii is unfocused in egg-laying time, long in egg-laying period, non-synchronous in maturity of male and female parent fishes and low in hatching rate. The artificial propagation method of the esox reichertii comprises the following steps of: sorting parents; and carrying out artificial impregnation after injecting the medicine twice by S-GnRH (gonadotropin releasing hormone)-A, HCG (Human Chorionic Gonadotropin) or DOM (Dimethoxy Mephentermine) in April, so that the artificial propagation of esox reichertii is completed. The artificial propagation method of the esox reichertii disclosed by the invention can be used for enabling the maturity of male and female parent fishes to be synchronous, enabling the egg-laying time to be focused and shortening the egg-laying period by 15-20 days, and also has a fertility rate of 85%-90% and a hatching rate of 60%-70%. The parent fishes are propagated artificially, the propagation success rate of male fishes is 97% and the propagation success rate of the female fishes is 70%-85%.

The present invention discloses a dedicated multi-index protein chip for female infertility detection and a kit thereof. The protein chip consists of a substrate, protein detection indexes arranged on the substrate in an array mode and a contrast coating for the detection indexes. The protein detection indexes cover ovary antigen (OAg), endometrium antigen (EAg), sperm antigen (SAg), zona pellucida antigen (ZAg), human chorionic gonadotropin antigen (HCG), trophoblast antigen (TAg) and cardiolipin antigen (CAg). The present invention overcomes the defects of the existing autoimmunological detection products on market, such as single detection function, lagged detection indexes and no integration. Besides the indexes of the product (patent application No: 200510102466.3), the protein chip also has the ACA index. The density of all indexes can be quantitatively detected, and the cross reaction of all indexes is eliminated. The present invention not only has high specificity and high sensitivity, but also has high accuracy and precision.

The invention discloses hyperglycosylated human growth hormone fusion protein. The human growth hormone fusion protein sequentially contains a human growth hormone (hGH), a flexible peptide joint (L), human chorionic gonadotropin beta-carboxyl terminal rigid peptide (CTP) and a human immunoglobulin Fc fragment from the N terminal to the C terminal. The invention further discloses a method for efficiently preparing the fusion protein. Compared with a recombinant hGH, the built fusion protein has more excellent in-vivo drug efficacy, the in-vivo circulation half-life period is prolonged, the administration frequency is greatly decreased, and the bioavailability is improved; meanwhile, the production process is simpler and more efficient.

The invention relates to an integrated detection reaction orifice plate and protein chip reagent box for detecting six sterility indexes. And the reaction orifice plate comprises a substrate and reaction orifices on the substrate and the reaction orifices comprise 3-384 sample orifices, 1-300 negative contrast orifices, and 1-300 positive quality control orifices, where at the bottom of each reaction hole is solid carrier, coated with micro lattice of Sperm antigen (SAg), Zona pellucida antigen (ZPAg), Endometrium antigen (EMAg), Ovary antigen (OAg), Trophoblast antigen (TAg), and Human Chorionic Gonadotropin antigen (HCGAg) antigens or more. And the reaction orifice plate and reagent can simply and conveniently, rapidly and accurately implement simultaneously detection of six sterility indexes for many persons.

The invention belongs to the field of clinical medical diagnosis and particularly relates to an HCG (human chorionic gonadotropin) colloidal gold immunoassay lateral chromatographic test strip and a detection method thereof. The HCG colloidal gold immunoassay lateral chromatographic test strip consists of a nitrocellulose film, a Fusion 5 film and water-sucking paper, wherein a detection line T line and a quality control line C line are arranged on the nitrocellulose film; a T line solution is sprayed onto the detection line T line; a C line solution is sprayed onto the detection line C line. A standard curve line is made through different concentration values and corresponding T / C average value of a standard product, the standard curve line is subjected to interpolation fit by sample strips for three times, the curved line which is relatively good in linearity and completely raises can be obtained, HOOK effect can be overcome, and detection on high-concentration HCG is realized. The detection range is up to 10 to 200,000mIU / ml, a to-be-detected sample is not required to be diluted, the detection process is simple and convenient, and the detection cost is low.

The invention relates to a method for detecting female ovulation and pregnancy, and discloses a female ovulation and pregnancy detection method. The detection method comprises the following steps: by virtue of a detection unit (10), detecting a temperature value of urine which is just discharged out of a woman, a change value of female body temperature in an ovulatory period as well a luteinizing hormone concentration value and a human chorionic gonadotropin concentration value in urine which is discharged in the ovulatory period every day; collecting a picture of a piece of pregnancy test paper by virtue of the detection unit (10), conducting color recognition on the picture, and finally, calculating a chromatic value of the color; saving the urine temperature value, the body temperature value within the ovulatory period, the chromatic value on the pregnancy test paper, the luteinizing hormone concentration value and the human chorionic gonadotropin concentration value in a first memory; transmitting the saved data to a processor by virtue of the first memory, and comparing the new data with historical data so as to determine an ovulation time and a pregnancy situation. With the implementation of the detection method disclosed by the invention, the ovulatory period can be precisely detected, so that people can prepare for pregnancy conveniently; and meanwhile, the pregnancy situation of the woman can be accurately judged as well.