38 results about "Swine urine" patented technology

The invention discloses a medical fertilizer special for orchid culture. The medical fertilizer consists of an organic fertilizer and an inorganic fertilizer, wherein the inorganic fertilizer is prepared from urea, triple superphosphate, potassium chloride, cupric sulfate pentahydrate, magnesium sulfate, ferrous sulfate, manganese sulfate, boric acid and zinc sulfate; and the organic fertilizer is prepared from dead leaves, pig manure, tobacco processing leftovers, fish product processing leftovers, wheat straws, a traditional Chinese medicine composition, swine urine and EM bacterial liquid. The organic fertilizer and the inorganic fertilizer are reasonably combined to serve as a base fertilizer for application to provide comprehensive nutrition for orchid and meanwhile, the inorganic fertilizer provides readily-available nutrients at first, the organic fertilizer provides long-acting and abundant N, P and K content, especially a relatively high potassium content to meet the requirement on the demand of potassium by the orchid in a later period and improve the disease resistance of the orchid at the same time. The traditional Chinese medicine composition of the medical fertilizer disclosed by the invention is combined with tobacco leftovers to achieve a good control effect on orchid root rot and orchid anthracnose, and can kill insects and termites.

The invention discloses an enzyme-linked immunosorbent assay (ELISA) kit for analyzing chloramphenicol residues based on a rabbit monoclonal antibody. The kit comprises a body and a 96-well ELISA plate and reagents arranged in the body, wherein a chloramphenicol envelop antigen is enveloped in the plate; and the reagents include (1) washing concentrate; (2) diluting concentrate; (3) chloramphenicol standard solution; (4) a chloramphenicol antibody; (5) a horseradish peroxidase labeled goat anti-rabbit antibody; (6) substrate diluent; (7) a substrate; (8) substrate development solution; and (9) reaction termination solution. The kit is applicable to detection of chloramphenicol residues in such samples as milk, swine urine, etc. The kit can simultaneously detect mass samples, is convenient and quick, is of great practical significance to field monitoring trace analysis, simultaneously greatly lowers the detection cost and has potential economic value.

The invention discloses a coconut waste culture medium for lentinus edodes cultivation. The coconut waste culture medium comprises the following raw materials: coconut water, coconut fiber, coconut shell powder, coconut shells, coconut cedar, swine urine, urea, gypsum, sugar beet pulp and tea leaf dregs. The invention further discloses a preparation method of the coconut waste culture medium for lentinus edodes cultivation. As coconut wastes are taken as main raw materials of the coconut waste culture medium, the cultivation cost is low, the coconut wastes can be reasonably matched with other components in the culture medium, the nutrient components are rich and comprehensive and can be easily absorbed and used by plants, the lentinus edodes is stable and high in yield and high in nutritional value, the contents of crude protein and amino acid in the lentinus edodes are higher than those of common lentinus edodes, very good economic benefits can be made, and the environment pollution can be reduced.

The invention relates to a method for rapidly detecting clenbuterol in swine urine based on a molecularly imprinted membrane. The clenbuterol and metabolite 3, 5-dichloro-para-aminophenol and 3, 5-dichloro-p-aminohippurate of the clenbuterol in the swine urine are simultaneously detected by a sensor array comprising the imprinted membrane and a quartz crystal microbalance. Compared with the prior art, the method has the advantages that the lower detection limit of an MIP-QCM sensor is 3 micrograms/L and lower than the upper specification limit 10 micrograms/L of a codex alimentarius commission, in stability tests, first 14 tests are stable, test results gradually rise from the 15 test and are about half of an initial state in the 20 test, finally, the method is rapid and accurate, the MIP-QCM sensor array respectively detect three types of solution, response values are subjected to clustering analysis by principal component analysis method, and 100% recognition rate can be obtained.

The invention provides a preparation method of enzyme-linked immunosorbent assay (ELISA) kit for detecting cyproheptadine hydrochloride. The kit is sensitive, accurate and rapid in detection, simple and convenient to operate, high in specificity and suitable for detecting samples in large batch, and comprises an ELISA plate coated with a cyproheptadine hydrochloride antigen, a cyproheptadine hydrochloride standard substance, a cyproheptadine hydrochloride enzyme-labeled antibody working solution, a substrate solution A, a substrate solution B, a stop solution and a concentrated scrubbing solution. According the principle of the kit, an ELISA reaction is performed through direct solid-phase competition; an extracted sample and the enzyme-labeled antibody working solution are added into a corresponding ELISA hole; after a period of incubation, the ELISA plate is scrubbed, and the substrate solution A and the substrate solution B are added; under the enzymatic action, a blue color appears in the ELISA hole; after the stop solution is added, the color changes from blue to yellow, and the color depth has an inversely proportional relationship with the content of cyproheptadine hydrochloride in the standard substance or the sample. The principle can be directly utilized for detecting the content of cyproheptadine hydrochloride in a swine urine sample.

The invention provides a rapid detection method of sulfadiazine in an animal body, and a Raman characteristic peak of sulfadiazine is determined by using a density functional theory. The method comprises a step of establishing a quantitative analysis curve according to the characteristic peak intensity with high Raman peak intensity and a good peak pattern. Standard recovery experiments show thatthe method is good in accuracy and precision. The lowest concentration of sulfadiazine in the animal body detected by the method is 2 mg/L. According to the method, the sulfonamide antibiotic residuesin the swine urine are qualitatively and quantitatively analyzed, single sample detection is completed within 3 min, and a rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfonamide antibiotic residues in the swine urine.

The invention discloses a method for preparing a liquid biological fertilizer from bamboo shoot processing wastes. The method comprises the following steps: crushing the bamboo shoot processing wastes to form powder, immersing the powder in a swine urine-containing potassium hydroxide solution in order to pre-wet the powder; mixing the pre-wetted bamboo shoot processing wastes, pig dung and a microbial agent, and carrying out sealing fermentation to obtain a fermented material; adding water to dilute the fermented material, stirring the fermented material and water, and carrying out suction filtration to obtain an extract liquid; and adding trace metal elements, humic acid, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate and a suspending agent to the extract liquid, stirring the extract liquid and above added materials until the extract liquid and the added materials are uniform, and adjusting the pH value of the above obtained mixture to obtain the above product. The biological fertilizer obtained through extracting trace nutrients, organic matters, humus and other nutrient substances from the wide-source bamboo shoot processing wastes used as a main raw material is environmentally-friendly, provides comprehensive nutrition for crops, improves the quality and the safety quality of the crops, and reduces environment pollution.

The present invention relates to the field of biotechnology, and discloses an anti-beta receptor agonist cluster-specific monoclonal antibody hybridoma cell line and a monoclonal antibody secreted bythe cell line. According to the present invention, the hybridoma cell line is prepared from salbutamol artificial antigen, and the secreted monoclonal antibody can simultaneously recognize salbutamol,clenbuterol, terbutaline, brombuterol, cimbuterol and other beta-receptor agonists, and has cluster specificity; the immunoaffinity chromatography gel prepared from the monoclonal antibody and the immunoaffinity chromatography gels of other beta-receptor agonists such as ractopamine are mixed as the machine measurement pretreatment purification reagent, a variety of clenbuterol hydrochloride components in pork, pig liver and pig urine samples can be efficiently detected by combining the machine measurement pretreatment purification reagent, HPLC-FLD and LC-MS/MS, and the high accuracy and high sensitivity can be achieved.

The invention provides a pigpen cushion ring and a use method thereof. The pigpen cushion ring comprises 0.05kg/m<2> of quicklime, 5-6kg/m<2> of corn straw, 1.5-2kg/m<2> of rice straw, 1-3kg/m<2> of peanut shell and 1-2kg/m<2> of charcoal. The peanut shell and the corn straw are good in water-absorbing quality, the charcoal can absorb the stink smell, the problem caused by swine urine and manure can be effectively solved, and the pigpen can keep dry, can be deodorized, is favorable for growth of pigs and has no effect on surrounding environment. Meanwhile, used cushion ring waste can be used as soil base fertilizer after fermentation.

The invention belongs to the fields of fertilizers and preparation of fertilizers and in particular relates to an organic matter. The organic matter is prepared from the following raw materials in percentage by weight: 30-69.9 percent of animal excreta, 5-30 percent of animal urine, 25-40 percent of multiple straw individuals or mixtures and 0.05-0.2 percent of core materials, wherein the animal excreta refers to cow dung or pig manure, the animal urine refers to bovine urine or swine urine, and the core materials refer to bacillus subtilis and bacillus licheniformis. The invention also discloses a process for preparing the organic matter. The process comprises the following steps: crushing the straws, tiling the straws on the ground, mixing the animal excreta and animal urine, adding the animal excreta and animal urine onto the tiled straws, stirring, stacking, turning, fermenting and the like, and preparing the high-quality organic matter from the raw materials. According to the organic matter disclosed by the invention, the animal urine can serve as the raw material, so that the excreta and urine of the animals can be fully utilized, and the pollution problem is solved.

The invention provides an enzyme linked immunosorbent assay (ELISA) kit for Cimaterol detection. The kit comprises an ELISA plate coated with a coating antigen, a Cimaterol standard substance solution, a Cimaterol antibody, an enzyme conjugate concentrate, an enzyme conjugate diluent, a substrate developing solution, a stop solution and a cleaning solution, wherein the coating antigen is a Cimaterol coupling antigen, an enzyme conjugate is the enzyme-labeled Cimaterol antibody, and the Cimaterol antibody is obtained from an immunogenic immune animal. The invention further discloses a method ofapplying the ELISA kit to Cimaterol detection. The method comprises the steps that first, sample pretreatment is performed; second, the kit is used for detection; and last, a detection result is analyzed. The ELISA kit can be used for detecting the content of Cimaterol in animal tissue and swine urine samples, is easy and convenient to operate, low in cost and high in sensitivity, can be monitored on spot and is suitable for screening of a large quantity of samples.

The invention discloses a method for measuring the content of ractopamine in swine urine at high precision. The method comprises the following steps: 1, pretreating a sample and collecting the swine urine; 2, adding supernate to an SLE (Supported Liquid Extraction) column and standing for 2 to 4 minutes; 3, eluting the SLE column for at least two times by adopting a binary eluent, and collecting an elution solution to obtain a ractopamine-containing elution solution; 4, detecting the elution solution obtained in the step 3 by adopting high performance liquid chromatography, wherein the binary eluent is a mixture of isopropanol and methyl acetate according to a weight ratio of (0.5 to 1): 1. The invention aims at providing the method for measuring the content of the ractopamine in the swine urine at high precision, which is high in separation efficiency and simple to operate.

The invention discloses a high-accuracy measuring method for the clenbuterol content in swine urine. The high-accuracy measuring method comprises the following steps of the step 1 of preprocessing samples, collecting swine urine, adding trichloroacetic acid to precipitate protein in the swine urine, adding 80%VOL 2mol/L ammonium acetate buffer solution, centrifuging the solution through a whirlpool mixer after being mixed and taking centrifuged liquid supernatant; the step 2 of adding the liquid supernatant into an SLE column and standing for 2 to 4 minutes; the step 3 of eluting the SLE column through binary eluent at least twice, receiving the eluent and obtaining the eluent containing clenbuterol; the step 4 of detecting the eluent obtained in the step 3 through a high-efficiency liquid chromatography. The high-accuracy measuring method for the clenbuterol content in the swine urine is good in separating effect and facilitates follow-up testing work.

The invention discloses a method for high-precisely measuring salbutamol in swine urine. The method comprises the following steps: step 1: pretreating a sample; collecting swine urine, firstly carrying out enzymolysis by charging beta-hydrochloric glucuronidase/aryl sulfatase, charging trichloroacetic acid to precipitate proteins in the swine urine, charging a phosphoric acid buffer solution with volume being 4 times of that of the swine urine and with pH value of 6.5 to 7.0, mixing by a vortex mixer, and then centrifuging; taking the supernatant of the centrifuged solution; step 2: charging the supernatant into an SLE column, and standing for 2 to 4 minutes; step 3: eluting the SLE column for at least two times by adopting a binary elution agent, collecting the elution solution, and obtaining the elution solution containing salbutamol; step 4: detecting the elution solution obtained in the step 3 by adopting a high-efficiency liquid-phase chromatography. The invention aims at providing the method for measuring the content of salbutamol in the swine urine, which is high in separation efficiency, simple to operate and high in precision.

The invention provides a pigpen cushion ring formula which comprises the following raw materials, by weight, 40-50 parts of wheat straw, 20-30 parts of peanut shell, 20-30 parts of sorghum straw, 10-20 parts of charcoal, 10-20 parts of wheat bran, 10-20 parts of rice bran and 10-20 parts of folium artemisiae argyi. The folium artemisiae argyi can repel mosquitoes and flies, the peanut shell, the sorghum straw, the wheat bran and the rice bran are good in water absorbing quality, the charcoal can absorb the stink smell, the problem caused by swine urine and manure can be effectively solved, and the pigpen can keep dry, can be deodorized, is favorable for growth of pigs and has no effect on surrounding environment. Meanwhile, used cushion ring waste can be used as soil base fertilizer after fermentation.

The invention provides a rapid detection method of sulfadimidine in an animal body. A Raman characteristic peak of the sulfadimidine is determined by using a density functional theory, a quantitativeanalysis curve is established according to the characteristic peak intensity with high Raman peak intensity and the good peak pattern, and adding standard recovery experiments show that the method isgood in accuracy and precision. The method detects that the minimum concentration in swine urine is the lowest concentration of the sulfadimidine and is 2 mg/L; and according to the method, sulfadimidine residues in the animal body are qualitatively and quantitatively analyzed, single sample detection is completed within 2 min, and the rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfadimidine residues in the animal body.

The invention provides a method for detecting RAC (ractopamine) based on electrical chemiluminescence. The content of RAC can be obtained according to a standard curve by detecting luminescence intensity values before RAC is added and after RAC is added. According to the invention, the method capable of detecting residues of RAC in swine urine fast and sensitively is established on the basis of an intense quenching reaction of RAC for a Ru(bpy)3<2+>/N-BDEA (butyldiethanolamine) chemiluminescent system. According to the method, according to a good linear dependence (a correlation coefficient r is equal to 0.9989) of the electrochemical luminescence intensity and the concentration of Ru(bpy)3<2+> within a range of 10<9>-10<5> g/mL, the lowest detection limit of RAC is 5*10<10> g/mL.

The invention discloses a manufacturing method for chicken manure compost. The manufacturing method comprises the following steps: selecting 300 to 350 parts of chicken manure, 200 to 250 parts of peanut shell, 100 to 150 parts of bagasse, 50 to 100 parts of fish viscera, 30 to 50 parts of swine urine, 100 to 150 parts of decayed leaves and 15 to 20 parts of lime; then subjecting the above materials to ordered mixing; and carrying out aerobic fermentation and discharging so as to obtain the finished chicken manure compost. The manufacturing method for the chicken manure compost is simple in preparation process; the chicken manure compost can rationally utilize the chicken manure and reduce environmental pollution; and with the chicken manure compost, the usage amount of chemical fertilizer is reduced, pollution to soil is mitigated, and the ecological environment of soil is optimized.

The invention discloses a method for separating salbutamol in pig urine by using the SLE method, which comprises the following steps: Step 1: sample pretreatment; collect pig urine, first add β-glucuronidase/arylsulfatase to enzymatically After enzymatic hydrolysis, add trichloroacetic acid to precipitate protein in pig urine, add phosphate buffer solution with 4 times the volume of pig urine with a pH range of 6.5 to 7.0, mix with a vortex mixer and centrifuge; take the supernatant after centrifugation; Step 2: After adding the supernatant to the SLE column, let it stand for 2 to 4 minutes; Step 3: Use the binary eluent to elute the SLE column at least twice, receive the eluate, and obtain the eluate containing albuterol . The object of the present invention is to provide a kind of method that adopts SLE method to separate albuterol in pig urine with high separation efficiency, simple operation and low detection limit.

The invention discloses a standard substance containing free-state and conjugated-state clenbuterol in swine urine and a preparation method of the standard substance. The method includes: feeding animals to obtain a swine urine raw material containing free-state and conjugated-state clenbuterol concentration ranges, fixing the value, and freeze-drying to obtain the free-state and conjugated-state clenbuterol standard substance in the swine urine freeze-dried powder of which the uniformity stability meets the requirements; adding water for redissolving into liquid, pre-treating the liquid by using a mixed biological enzyme enzymolysis method, and determining the content of clenbuterol in the matrix standard substance by using a liquid chromatography-tandem mass spectrometry/isotope internal standard method for combined valuing. The standard substance provided by the invention reflects the real condition of the clenbuterol standard substance in swine urine metabolized in an animal body, the fixed value is more accurate, and the uniformity and the stability are good; therefore, the standard substance can be widely applied to many fields of clenbuterol residue detection in animal urine, quality verification of rapid screening products, instrument detection method evaluation, laboratory internal quality control, laboratory capability verification and comparison and the like.

The invention discloses a method for measuring the contents of ractopamine, clenbuterol and salbutamol in swine urine at high precision. The method comprises the following steps of 1, pretreating a sample, namely collecting swine urine, firstly adding beta-glucuronidase hydrochloride/arylsulfatase to carry out enzymolysis, and then, extracting a centrifuged supernatant; 2, adding the supernatant into an SLE column, and then, standing for 2-4 minutes; 3, eluting the SLE column at least twice by using a binary eluting agent, and receiving an eluant to obtain an eluant containing ractopamine, clenbuterol and salbutamol; and 4, detecting the eluant obtained in the step 3 by using a high-performance liquid chromatography. The invention aims at providing the method for measuring the contents of ractopamine, clenbuterol and salbutamol in the swine urine at high precision.

The invention discloses a standard substance containing free-state and conjugated-state salbutamol in pig urine freeze-dried powder after animal metabolism and a preparation method of the standard substance. The preparation method comprises: feeding animals to obtain a swine urine raw material containing free-state salbutamol and conjugated-state salbutamol in a concentration range, fixing the value, and freeze-drying to obtain the free-state salbutamol and conjugated-state salbutamol standard substance in the swine urine freeze-dried powder of which the uniformity stability meets the requirements; adding water for redissolving into a liquid, performing pretreatment by a mixed biological enzyme enzymolysis method and performing combined valuing by a liquid chromatography-tandem mass spectrometry/isotope internal standard method to determine the content ofsalbutamol in the standard substance. The standard substance prepared by the method reflects the real condition of the salbutamol standard substance in swine urine metabolized in an animal body, the fixed value is more accurate, and the uniformity and the stability are good; besides, the method can be widely applied to multiple fields of salbutamol residue detection in animal urine, quality verification of rapid screening products, instrument detection method evaluation, laboratory internal quality control, laboratory capability verification and comparison and the like.

The invention discloses a liquid-state fertilizer additive prepared from farm waste and a preparation method of the liquid-state fertilizer additive. The liquid-state fertilizer additive is prepared from the following raw materials in parts by weight: sodium citrate, dried tangerine peel dreg, citric acid, agricultural rare earth, methylene succinic acid, ethyl borate, swine urine, duck beds, dried chicken manure, pig farm rare earth, an EM fungicide, aluminum sulfate, a 65%-75% ethanol solution, a plant additive and a proper amount of water. Subsoil of a farm for poultries and the like contains wastes of a plurality of poultry excrements, and the wastes are different from direct excrements and are subjected to metabolism and decomposing by soil microorganisms in soil, so that toxins in the excrements are lost or decomposed, and macromolecule celluloses which are not completely digested are almost split and are more easily utilized, propagated and decomposed by microorganisms than other organic matters; and microorganism proliferation is firstly carried out through aerobic fermentation, then the organic matters are decomposed, and finally, microbial cells are broken and contain a plurality of substances which are beneficial for the growth of plants and very useful for the growth of crops.

The invention relates to a method for detecting the residual quantity of sulfonamides in swine urine by ultra-high performance liquid chromatography-electrospray tandem mass spectrometry. The method comprises the following steps: sucking 5.00 mL of a sample, placing the sample in a 50 mL centrifuge tube, adding 5 g of anhydrous sodium sulfate and 15 mL of an acetonitrile solution, carrying out ultrasonic extraction for 3 min, and centrifuging at 5000 r/min for 5 min; pouring the supernatant into another centrifuge tube, adding 15 mL of acetonitrile into the residue, and repeating the operationonce. Mixing extracting solutions, blowing nitrogen to be nearly dry at 50 DEG C, accurately adding 1.00 mL of dissolved residues, filtering through a 0.22 mm filter membrane, and determining by using a liquid chromatography-tandem mass spectrometer.

The invention discloses a ractopamine standard substance coexisting in a conjugated state and a free state in a swine urine matrix as well as a preparation method and application of the ractopamine standard substance. The method includes: feeding animals with ractopamine to obtain a pig urine raw material, and valuing to obtain the free-state and conjugated-state coexisting ractopamine standard substance in the pig urine freeze-dried powder; adding water for redissolving into liquid, pre-treating the liquid by using a mixed biological enzyme enzymolysis method, and determining the content of ractopamine in the matrix standard substance by using a liquid chromatography-tandem mass spectrometry/isotope internal standard method for combined valuing. The standard substance prepared by the method provided by the invention can truly reflect the coexistence condition of the ractopamine standard substance in swine urine in a conjugated state and a free state after in-vivo metabolism of animals, and is more accurate in fixed value and good in uniformity and stability than a temporarily added and prepared standard substance in on-site rapid detection; therefore, the standard substance can be widely applied to the fields of ractopamine residue detection in animal urine, quality verification of rapid screening products, instrument detection method evaluation, laboratory internal quality control and the like.