Method for rapidly detecting clenbuterol in swine urine based on molecularly imprinted membrane

A molecularly imprinted membrane, pig urine technology, applied in measuring devices, analytical materials, and fluid analysis using sonic/ultrasonic/infrasonic waves, etc., can solve the problems of high instrument cost, slow analysis speed, false positives, etc., and achieve high selectivity. , The effect of low detection limit and short time-consuming

Inactive Publication Date: 2017-01-04
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, GC-MS and HPLC methods can perform accurate measurement, but they have disadvantages such as high instrument cost and slow analysis speed; ELISA method...

Method used

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  • Method for rapidly detecting clenbuterol in swine urine based on molecularly imprinted membrane
  • Method for rapidly detecting clenbuterol in swine urine based on molecularly imprinted membrane
  • Method for rapidly detecting clenbuterol in swine urine based on molecularly imprinted membrane

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Experimental program
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Effect test

Embodiment 1

[0054] The method for the rapid detection of clenbuterol in pig urine based on molecularly imprinted membranes adopts the following steps:

[0055] 1 Determination of the optimal ratio of template molecules to functional monomers

[0056] 1.1 First, draw the 2D structure of the template molecule and functional monomer, and then convert it into a 3D molecular model. Two-step optimization was performed by using the molecular mechanics (MM) method and AM1 (Austin Method 1) semi-empirical quantification method.

[0057] 1.2 After the calculation, the energy of the template molecule, the energy of the functional monomer and the respective charge distribution can be obtained respectively. Finally, the template molecules and functional monomers in different proportions were arranged according to the charge distribution and hydrogen bond rules, and then the AMBER MM method and the AM1 method were used in turn to obtain the lowest energy conformation of the complex. Determine the opt...

Embodiment 2

[0087] The method for the rapid detection of clenbuterol in pig urine based on molecularly imprinted membranes adopts the following steps:

[0088] (1) Use the molecular simulation software Hyperchem 7.5 to determine the optimal ratio of template molecules to functional monomers, specifically using the following methods:

[0089] (1-1) Draw the 2D structures of template molecules and functional monomers, and then convert them into 3D molecular models, and optimize the two-step optimization by molecular mechanics methods and semi-empirical quantification methods;

[0090] (1-2) After the calculation, the energy of the template molecule, the energy of the functional monomer and the respective charge distribution are obtained, and then the different proportions of the template molecule and the functional monomer are arranged according to the charge distribution and hydrogen bond rules, and then the molecular mechanics method is used in turn The lowest energy conformation of the c...

Embodiment 3

[0106] The method for the rapid detection of clenbuterol in pig urine based on molecularly imprinted membranes adopts the following steps:

[0107] (1) Use the molecular simulation software Hyperchem 7.5 to determine the optimal ratio of template molecules to functional monomers, specifically using the following methods:

[0108] (1-1) Draw the 2D structures of template molecules and functional monomers, and then convert them into 3D molecular models, and optimize the two-step optimization by molecular mechanics methods and semi-empirical quantification methods;

[0109] (1-2) After the calculation, the energy of the template molecule, the energy of the functional monomer and the respective charge distribution are obtained, and then the different proportions of the template molecule and the functional monomer are arranged according to the charge distribution and hydrogen bond rules, and then the molecular mechanics method is used in turn The lowest energy conformation of the c...

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Abstract

The invention relates to a method for rapidly detecting clenbuterol in swine urine based on a molecularly imprinted membrane. The clenbuterol and metabolite 3, 5-dichloro-para-aminophenol and 3, 5-dichloro-p-aminohippurate of the clenbuterol in the swine urine are simultaneously detected by a sensor array comprising the imprinted membrane and a quartz crystal microbalance. Compared with the prior art, the method has the advantages that the lower detection limit of an MIP-QCM sensor is 3 micrograms/L and lower than the upper specification limit 10 micrograms/L of a codex alimentarius commission, in stability tests, first 14 tests are stable, test results gradually rise from the 15 test and are about half of an initial state in the 20 test, finally, the method is rapid and accurate, the MIP-QCM sensor array respectively detect three types of solution, response values are subjected to clustering analysis by principal component analysis method, and 100% recognition rate can be obtained.

Description

technical field [0001] The invention belongs to the rapid detection technology of food safety, in particular to a method for the rapid detection of clenbuterol in pig urine based on molecularly imprinted membranes, which can provide sensor array support for the development of an intelligent electronic tongue for rapid detection of clenbuterol components . Background technique [0002] At present, acoustic wave sensors have been able to detect certain target analytes in a targeted manner. This technology has been widely used in the fields of environmental detection, chemical substance production and organic compound synthesis. Recently, multiple sensors with specific recognition capabilities have been combined into an array to detect the target solution. Sensor arrays are commonly used in biological systems to detect odors. The biggest advantage of the sensor array approach is that it can turn some sensor elements with poor selectivity and severe cross-reactivity into a set...

Claims

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Application Information

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IPC IPC(8): G01N29/036
CPCG01N29/036G01N2291/022
Inventor 赵大云郑建武冯凡韩韬陈景
Owner SHANGHAI JIAO TONG UNIV
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