The present invention discloses a sulfanilamide drug chemiluminescence enzyme-linked immunodetection kit, which comprises a kit body, an enzyme label plate placed inside the kit body, and reagents placed inside the kit body, and is characterized in that every hole of the enzyme label plate is coated with coating antigen, the coating antigen is a sulfanilamide mother nucleus and carrier protein conjugate, and the reagents comprise sulfanilamide monoclonal antibody, horseradish peroxidase-labeled goat anti-mouse antibody, a series of sulfanilamide standard solutions, a concentrated phosphate buffer, a concentrated washing solution and a chemiluminescence solution. The sulfanilamide drug chemiluminescence enzyme-linked immunodetection kit has characteristics of high sensitivity, simple and rapid detection, high accuracy, and more drug detection types, provides a substantially reduced operation time compared to the conventional colorimetric ELISA method, and can be used for detection of residues of the 17 sulfanilamide drugs in animal tissues (pork, chicken, pork liver and chicken liver), aquatic products (fish and shrimp), eggs, milk and milk powder.