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Melamine and carrier protein couplet product, preparation method and uses of melamine antibody

A technology of melamine and carrier protein, applied in chemical instruments and methods, ovalbumin, albumin peptides, etc., can solve the problems of cumbersome operation, expensive instruments, time-consuming and laborious, etc., and achieve the effect of simple and convenient operation and simple and effective method.

Inactive Publication Date: 2009-05-13
ZHEJIANG UNIV
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Problems solved by technology

[0006] At present, the detection of melamine residues mainly contains two methods: instrumental analysis and immunoassay. Among them, instrumental analysis mainly uses high-performance liquid chromatography (HPLC) and gas chromatography (GC). High, incapable of large-scale on-site detection and other issues, also requires technicians to have certain operation and analysis skills, so it cannot be well popularized and applied

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  • Melamine and carrier protein couplet product, preparation method and uses of melamine antibody
  • Melamine and carrier protein couplet product, preparation method and uses of melamine antibody

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Embodiment Construction

[0032] The invention provides a preparation method and application of a melamine-carrier protein coupling product and a melamine antibody. Use the prepared immunogen to prepare monoclonal antibodies and polyclonal antibodies with strong specificity, high sensitivity, good stability, and large-scale production of melamine, and use these antibodies to establish the detection of melamine residues in food with high specificity, sensitivity and correctness The rapid diagnostic method - competitive ELISA and immunochromatographic test strips, can be effectively used in the detection of melamine residues in food, and serve the food safety of our country.

[0033] 1. a kind of preparation method of melamine and carrier protein coupling product comprises the steps:

[0034] a) 3 mg of melamine was dissolved in 1 mL of dimethylformamide / 1,4-dioxane / 0.1 MpH7.2 PBS solution with a volume ratio of 1:1:2;

[0035] b) 2 mg of carrier protein, bovine serum albumin (BSA), was dissolved in 1 m...

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Abstract

The invention discloses a preparation method and application of a product obtained by coupling melamine with carrier protein, as well as a melamine antibody. The product obtained by coupling the carrier protein with the melamine is used as artificial antigen and applied to an immunological method for melamine detection. The preparation method comprises the following steps of immunizing animals with the coupled product so as to prepare an antibody used for melamine detection, fusing BALB / C mouse spleen cells immunized with the coupled product and SP2 / 0 mouse myeloma cells, obtaining hybridoma capable of stably transferring culture and secreting anti-melamine specific monoclonal antibodies by screening positive hybridoma and cloning cells, and preparing an ascites monoclonal antibody. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the melamine, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling melamine with carrier protein, as well as the melamine antibody provides service for the rapid detection of melamine-type residue in foods.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and biology, and in particular relates to a preparation method and application of a coupling product of melamine and carrier protein and melamine antibody. Background technique [0002] Melamine is referred to as triamine for short, and its scientific name is triaminotriazine, and its aliases are melamine, cyanuramide, and trimeramide. Molecular formula: C3N6H6, C3N3(NH2)3. Molecular weight: 126.12, is an important nitrogen heterocycle organic chemical raw material. Melamine is weakly alkaline and can react with various acids to form melamine salts. In strong acid or strong lye, melamine is hydrolyzed, and the amino groups are gradually replaced by hydroxyl groups to generate ammeline, ammeline and cyanuric acid. Melamine reacts with aldehydes to form addition compounds. Melamine is reacted with aldehydes to form a resin. Melamine resin is a versatile material that is fire and heat resi...

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Application Information

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IPC IPC(8): C07K14/795C07K14/77C07K14/765C07K16/00C07K16/44C07K16/06G01N33/577
Inventor 吴建祥张少恩
Owner ZHEJIANG UNIV
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