The invention relates to the medical field of
immunoassay, more specially, the invention provides a
cytokeratin 19 fragment
chemiluminescent immunoassay detection kit and a preparation method thereof. The kit of the invention comprises 1)
cytokeratin 19 fragment CYFRA21-1 calibrators, 2)
solid-phase vectors which are coated by
cytokeratin 19 fragment CYFRA21-1
monoclonal antibodies, 3) cytokeratin 19 fragment CYFRA21-1
monoclonal antibodies which are marked by
enzyme and 4) chemiluminescent substrates which are acted by the
enzyme. Further, the preparation method of the kit according to the invention comprises the following steps: 1) preparing the cytokeratin 19 fragment CYFRA21-1 calibrators, 2)
coating the
solid-phase vectors with the cytokeratin 19 fragment CYFRA21-1
monoclonal antibodies, 3) marking the cytokeratin 19 fragment CYFRA21-1 monoclonal antibodies with the
enzyme, 4) packaging the calibrators, the enzyme markers and the chemiluminescent substrates and 5) assembling finished products. The kit of the invention has the advantages of convenience, rapidness, sensitivity, stability and the like.