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Molecular diagnosis method for rapid detection of bactrocera dorsalis ovum in fruits

A technology of Bactrocera dorsalis and molecular diagnosis, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2010-08-04
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

There are few research reports on species identification and population differentiation of Bactrocera dorsalis using molecular biology techniques in my country

Method used

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  • Molecular diagnosis method for rapid detection of bactrocera dorsalis ovum in fruits
  • Molecular diagnosis method for rapid detection of bactrocera dorsalis ovum in fruits
  • Molecular diagnosis method for rapid detection of bactrocera dorsalis ovum in fruits

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Embodiment Construction

[0022] A molecular diagnostic method for the rapid detection of Bactrocera dorsalis eggs in fruit. It is of great significance to apply molecular biology techniques to the molecular rapid identification of Bactrocera dorsalis, especially for the molecular rapid identification of Bacteralis dorsalis eggs that cannot be detected visually.

[0023] Based on the purpose of quickly detecting Bactrocera dorsalis eggs in fruits, this experiment studied a set of nested PCR to amplify the ITS interrupted sequence in Bactrocera dorsalis rDNA. The test results show that the method is reliable and can quickly detect fruit The eggs of Bacteralis dorsalis were found in the sample, and the evolutionary relationship of the samples was determined by sequence analysis.

[0024] A set of nested PCR amplifies the ITS interrupted sequence in Bacteralis dorsalis rDNA, making:

[0025] 1. Test materials and methods:

[0026] 1.1 Test samples and source of insects

[0027] Cut off the outer skin o...

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Abstract

The invention relates to a molecular diagnosis method for the rapid detection of a bactrocera dorsalis ovum in fruits, which adopts a nested type PCR to amplify ITS break sequence in bactrocera dorsalis rDNA, amplifies specific ITS segments in the bactrocera dorsalis rDNA through designing a nested type specific primer to accurately and rapidly identify the bactrocera dorsalis ovum in the fruits, and determines the differences of the ovum and the resembling variety thereof. The method has reliable result, can rapidly detect the existence of the bactrocera dorsalis ovum in the fruits, and determine the species evolution relation of the sample through sequence analysis.

Description

technical field [0001] The invention belongs to biological detection technology, in particular to a molecular diagnosis method for rapidly detecting eggs of Bactrocera dorsalis in fruits. Background technique [0002] Bactrocera dorsalis (Hendel) is a quarantine pest that directly endangers the production of fruits and vegetables and has an important impact on export trade. Dangerous fruit and vegetable pests are one of the main targets of plant quarantine in my country. At present, the port quarantine and identification of Bactrocera dorsalis is mainly based on the external morphological characteristics of the adults, and the larvae or eggs are often intercepted at the port. The usual method is to rear them indoors to obtain adults before identification. [0003] With the rapid development of molecular biology, polymerase chain reaction (PCR) technology, nucleic acid sequence analysis technology and other molecular technologies have been widely used in taxonomy, systematics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 孔秋莲戚文元叶军岳玲陈志军
Owner SHANGHAI ACAD OF AGRI SCI
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