49 results about "Molecular Diagnostic Method" patented technology

The present invention describes methods of molecular diagnosis of a concrete form of a-synucleinopathy, the dementia with Lewy bodies (DLB), associated to the levels of ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) or the alteration of its ubiquityl-ligase activity. It also refers to the use of compounds that permit the modification of the UCH-L1 levels or of the enzymatic activity of UCH-L1. This invention has application in the diagnosis and treatment of patients suffering from DLB.

Lateral flow analysis strips and molecular diagnostic methods using the same are disclosed. The lateral flow analysis strip may comprise a sample pad (100) into which a sample comprising at least one of an amplicon labeled with a label and an amplicon precursor labeled with a label is to be introduced; a conjugate pad (200) comprising a conjugate (C1), the conjugate (C1) having an indicator and a detection agent bound to a surface of the indicator, and the conjugate (C1) being non-fixedly adsorbed to the conjugate pad (200); a test pad (300) comprising a test line (310) to which a first trapping agent is fixed; a control pad (400) comprising a control line (410) to which a second trapping agent is immobilized; and an absorbent pad (500). The lateral flow analysis strip of the present invention can detect amplicons (e.g., nucleic acids) amplified by using an amplicon precursor labeled with a type of label (e.g., a primer or dNTP) and can determine the results directly with the naked eye. Furthermore, the lateral flow analysis strip advanlabel eously has high sensitivity by using the label having a strong binding force, the trapping agent bound to the label , a detection agent, and a trapping agent bound to the detection agent. Further, by using the label having a certain binding force, the trapping agent bound to the label, the detection agent, and the trapping agent bound to the detection agent, the lateral flow analysis strip has reproducible and stable effects.

Proposed are an integrated kit for in-situ molecular diagnosis and a molecular diagnosis method using the same. The integrated kit for on-site molecular diagnosis comprises a first assembly and a second assembly, the first assembly comprises a sample pretreatment part, a nucleic acid absorption part and a nucleic acid amplification part, and the second assembly comprises a detection part. The sample pretreatment section includes a transfer member configured to transfer a lysis buffer in which nucleic acid is dissolved, and is configured to pretreat a sample. The nucleic acid absorption portion is positioned on the sample pretreatment portion and configured to be slidable onto the nucleic acid amplification portion. The nucleic acid amplification part is connected to the sample pretreatment part, and is configured to elute the nucleic acid from the nucleic acid absorption part and amplify the eluted nucleic acid. The detection section is configured to transfer the nucleic acid amplified by the nucleic acid amplification section and detect the nucleic acid. The integrated kit for in-situ molecular diagnosis and the molecular diagnosis method using the same according to the present disclosure are advantageous in that the kit enables ordinary people to perform molecular diagnosis without specialized devices (such as a centrifuge or a pipettor), ensures low-pollution operation, and has cost effectiveness.