Kit for predicting prostate screening and lymphatic metastasis
A technology for prostate cancer and lymphatic metastasis, applied in the field of biomedicine, can solve the problems of inability to distinguish other tumors, the methylation site is not specific enough, and insufficient to distinguish prostatic hyperplasia and prostatitis, and achieves high clinical positive predictive value and negative Predictive value and detection result are accurate and reliable
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Embodiment example 1
[0037] Implementation Case 1: Urine from 50 inpatients (not less than 10ml) from the Urology Department of Shanghai Tenth People’s Hospital, including 25 bladder cancer patients, 11 prostate patients, and the rest are non-tumor patients of the urinary system such as benign prostatic hyperplasia, prostatic hyperplasia Inflammation, urethritis and other patients. Through the technical scheme of the present invention, the following primer-probe combinations are used for detection: KDM5D (FAM fluorescence), EFEMP1 (VIC fluorescence), PITX2 (ROX fluorescence), RASSF1 (CY5 fluorescence), GSTP1 (NED fluorescence), all using high hybridization For MGB probes with high resolution, run the program on ABI7500: 50°C for 2min, 95°C for 5min, 95°C for 10sec, 60°C for 40sec, cycle 40-45 times, 5-color fluorescence detection. In addition, the detection of monochromatic fluorescence (both labeled with FAM) can achieve the same effect, but the nucleic acid template needs to be detected in sever...
Embodiment example 2
[0059] Example 2: 38 urine samples (not less than 10ml) from the Urology Department of Shanghai Pudong Hospital, including 9 bladder cancer patients, 20 prostate patients, and 9 non-tumor patients in the urinary system. Through the technical scheme of the present invention, use the following primer probe combinations to detect, CRMP4 (FAM), WFDC2 (VIC), TACSTD2 (NED), CYBA (CY5), all adopt the MGB probe of high hybridization resolution, run on ABI7500 Program: 50°C for 2min, 95°C for 5min, 95°C for 10sec, 60°C for 40sec, cycle 40-45 times, 4-color fluorescence detection.
[0060] Test results such as Figure 2-1 to Figure 2-4 : 18 out of 20 prostate patients were detected positive, while 9 bladder cancer patients and 9 non-tumor patients were all negative. The positive predictive value is 100%, and the negative predictive value is 90%. In addition, lymph node metastasis was predicted according to the positive detection of CRMP4: 8 cases had lymph node metastasis in 18 cases,...
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