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Molecular diagnosis method for evaluating growth traits based on copy number variation (CNV) marker of cattle ZNF146 gene and application of molecular diagnosis method

A technology of growth traits and genes, which is applied in biochemical equipment and methods, measurement/testing of microbes, DNA/RNA fragments, etc., can solve problems such as detection of cattle that have not been seen, and achieve the effect of simple operation, accurate and reliable method

Active Publication Date: 2021-07-23
河南省畜牧总站
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Problems solved by technology

[0005] So far, there have been no literature reports on the detection of the copy number variation of the ZNF146 gene in cattle, and the impact of CNV of the ZNF146 gene on the growth traits of local yellow cattle and other selective breeding cattle breeds

Method used

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  • Molecular diagnosis method for evaluating growth traits based on copy number variation (CNV) marker of cattle ZNF146 gene and application of molecular diagnosis method
  • Molecular diagnosis method for evaluating growth traits based on copy number variation (CNV) marker of cattle ZNF146 gene and application of molecular diagnosis method
  • Molecular diagnosis method for evaluating growth traits based on copy number variation (CNV) marker of cattle ZNF146 gene and application of molecular diagnosis method

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Embodiment Construction

[0035] The invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are explanations of the present invention, rather than limiting the protection scope of the present invention.

[0036] In the previous bovine genome resequencing study, it was found that ZNF146 had a copy number variation in the bovine genome reference region Chr 18:47225201bp-47229600bp. Combining the physiological role of the ZNF146 gene and the regulatory mechanism of CNV, the copy number variation of the ZNF146 gene was further revealed The association with the growth traits of cattle provides an important basis for the molecular breeding of local yellow cattle and related breeding varieties. The details are as follows.

[0037] 1. Sample collection and genomic DNA extraction

[0038] (1) Collection of blood samples

[0039] Table 1. Test animal sample information

[0040]

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Abstract

The invention discloses a molecular diagnosis method for evaluating growth traits based on a copy number variation (CNV) marker of a cattle ZNF146 gene and application of the molecular diagnosis method. Based on real-time quantitative polymerase chain reaction (PCR), by taking cattle genome DNA to be detected as a template, a CNV region of the ZNF146 gene and a partial fragment of the internal reference gene BTF3 are amplified by using two pairs of primers P1 and P2, and finally, a copy number variation type of an individual is calculated and judged by using a 2*2<-delta delta Ct> method. The invention can detect the CNV marker closely related to the cattle growth traits on a DNA level, and the method is simple, is rapid and is convenient to apply and popularize.

Description

technical field [0001] The invention belongs to the field of livestock molecular biology detection, in particular to a method for detecting variation in the copy number of the bovine ZNF146 gene by qPCR, which can be based on 2*2 -ΔΔCt Determine the type of copy number variation in an individual. Background technique [0002] Copy number variation (Copy Number Variations, CNVs) refers to the insertion or deletion variation of a genome sequence greater than 50 bp between two individuals of a species, which is a type of genome structure variation. It can affect the function of genes as well as the phenotype of individuals through dose effects, position effects, blocking functional genes, fusion genes, exposure of recessive alleles and potential transition effects. With the completion of the whole genome sequencing of cattle, the study of CNVs in bovine genome has also become a hot spot. [0003] Studying the molecular mechanism regulating the growth and muscle development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/166C12Q2531/113C12Q2537/16C12Q2563/107C12Q2545/101
Inventor 刘贤黄永震丁晓婷蔡雯雯徐美芳丁涵彭巍黄殷琪李欣淼吴胜军李志明张子敬王二耀茹宝瑞
Owner 河南省畜牧总站
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