346 results about "Phytophthora capsici" patented technology

The invention relates to bacillus amyloliquefaciens which has stronger phosphate solubilizing and plant growth promoting functions and has a better preventing and treating effect on various plant diseases. According to the bacillus amyloliquefaciens JCD-H-12 with the phosphate solubilizing, disease preventing and growth promoting functions, disclosed by the invention, the preservation number is CGMCC NO.11856, the preservation date is December 10, 2015, and multiple effects of solubilizing phosphate, preventing diseases, promoting the growth, increasing the yield and the quality, having a broad bacteriostatic spectrum and the like are obtained; a bacterial strain and inoculant of the bacterial strain are capable of effectively preventing and treating powdery mildew, tobacco black shank, botrytis blight, rice blast, cucumber fusarium wilt, tomato early blight, sweet pepper anthracnose, watermelon sclerotinia sclerotiorum, wheat scab, watermelon fusarium wilt, phytophthora capsici and the like; a broad-spectrum antibacterial effect is obtained, the effect is stable and durable, and the bacillus amyloliquefaciens can be developed into a bacterial fertilizer and microbial pesticide preparation which integrates phosphate solubilizing with disease preventing and treating.

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention discloses a trichoderma asperellum strain and application thereof. The trichoderma asperellum strain is named as trichoderma asperellum Thz01, and the preservation number of the trichoderma asperellum strain is CGMCC (China general microbiological culture collection center) No.6422. The trichoderma asperellum strain is separated from a capsicum habitat, has a remarkable antagonism effect to phytophthora capsici, can be obviously subjected to hyperparasitism on the phytophthora capsici, an can effectively degrade mycelia of the phytophthora capsici, and a biocontrol agent prepared from the strain can be used for effectively preventing and treating phytophthora capsici leonian .

The invention discloses a method for generating sporangia and releasing zoospore by inducing phytophthora capsici. The method includes the steps that phytophthora capsici is activated, an inducing solution for generating sporangia is prepared, sporangia are induced to be generated, and zoospore is induced to be released. The inducing solution used in the method is originally imported V8 vegetable juice with the volume concentration being 10-15% and a CaCO3 solution with the volume concentration being 0.2-0.3 g/L. When phytophthora capsici is induced to generate the sporangia, continuous light culture is carried out under a white fluorescent lamp. The method is simple, convenient to use, quick, free of pollution and capable of being used in phytophthora capsici pathogenicity measurement and biological activity measurement, achieved through an antibacterial agent, of phytophthora capsici.

Cyazofamid belongs to sulfanilamide imidazole bactericide and has high bioactivity on oomycete fungi such as phytophthora capsici, downy mildew, pseudo downy mildew and pythium and brassica plasmodiophora brassicae of plasmodiophoromycetes. A preparation method for cyazofamid aqueous emulsion has a formulation comprising 1 to 30 percent of the cyazofamid, 2 to 15 percent of emulsifying agent, 0.2 to 5 percent of co-emulsifying agent, 10 to 20 percent of solvent, 10 to 20 percent of latent solvent, 0.1 to 5 percent of anti-freezing agent, 2 to 10 percent of stabilizing agent, 0.1 to 2 percent of defoaming agent, 0.1 to 5 percent of thickening agent, 0.1 to 4 percent of pH value conditioner and the balance of deionized water, and comprises the following steps: adding the latent solvent into the deionized water according to a ratio; dispersing the latent solvent in a high-shear preparation kettle and stirring the solution evenly; adding cyazofamid raw powder and solvent as well as latent solvent mother solution into the solution; shearing the mixed solution at high speed; and finally adding the thickening agent into the mixed and stirring the resulting solution evenly to obtain the cyazofamid aqueous emulsion. The cyazofamid aqueous emulsion is a new environment-friendly water-based dosage form of agricultural bactericide, uses water as a dispersive medium to replace an expensive organic solvent, reduces environment and dust pollution, and is a new dosage form with development prospect.

The invention discloses a Rhodopseudomonas strain. The name of the Rhodopseudomonas strain is Rhodopseudomonas PSB-06, and the collection number is CCTCC NO:M2012518. The invention also discloses a biocontrol microbial inoculum which is obtained through the activation, seed culture and production culture of the strain. The preparation of the biocontrol microbial inoculum comprises the multiple steps of activation, seed culture and production culture. The invention also discloses a Rhodopseudomonas strain biocontrol fermentation liquid which is prepared from the biocontrol microbial inoculum through fermentation and centrifugation. Each of the Rhodopseudomonas strain, the biocontrol microbial inoculum and the biocontrol fermentation liquid can be used in antagonizing phytophthora capsici or controlling phytophthora blight of pepper.

The invention discloses a compound biological preservative. The compound biological preservative comprises the components as follows: 0.1-5 g of component B is added in each 1L of component A, the component A is fermentation liquor of Bacillusamyloliquefacienssubsp.plantarum BGP20 strains, and the component B is natamycin. The compound biological preservative is used for preventing and controlling diseases of fruits and vegetables in a storage period and particularly can be used for simultaneously preventing and treating bacterial soft rot and various fungal diseases (for example, phytophthora capsici, rhizopus soft rot, pythium blight and the like) of fruits and vegetables in the storage period, avoiding or reducing residual chemical pesticides and agricultural antibiotics and maintaining the quality of the fruits and vegetables in the storage period, so that the compound biological preservative has good economic and social benefits.

The invention discloses streptomyces antibioticu and biological preparation prepared from an active product thereof and application. The streptomyces antibioticu is a strain which is screened from more than 2,000 marine actinomycetes and has good inhibiting activity on phytophthora capsici, phytophthora cucumisativus, colletorichum cucumisativus and colletotrichum capsici; and the name of the strain is streptomyces antibioticu H74-18, the strain was collected in the China Center for Type Culture Collection on December 27th, 2010, and the collection number is CCTCC NO: M2010364. Antimycin A18(1) with bacteriostatic activity is separated from the strain, the molecular formula of the antimycin A18(1) is C28H41N2O9, and the molecular weight of the antimycin A18(1) is 549.3. The antimycin A18(1), an emulsifier and methanol are mixed to form the biological preparation with good stability. In 12 days after the biological preparation is sprayed, under the application times of 500x, the effect of preventing and controlling the phytophthora capsici is 90.00 percent, the effect of preventing and controlling the phytophthora cucumisativus is 86.67 percent, the effect of preventing and controlling the colletotrichum capsici is 86.67 percent and the effect of preventing and controlling the colletorichum cucumisativus is 90.00 percent.

The invention relates to a fluorescence quantitative polymerase chain reaction (PCR) detection method and a detection kit for phytophthora capsici leonian. In the method, a primer pair ljym1/ljym2 is designed according to an ITS area of the phytophthora capsici leonian rDNA; the reaction liquid contains the pair of specific primers and fluorescent dye; a phytophthora capsici leonian DNA template is added into the reaction liquid; and the fluorescence intensity of the reaction system is detected in real time along with the PCR reaction, and a Ct value is calculated according to analysis software so as to calculate the content of phytophthora capsici leonian in a sample. The kit designed according to the method has simple and fast operation and good specificity and high sensitivity, is free from the culture conditions based on the nucleotide detection, can actually reflect the infection conditions of phytophthora capsici leonian into plants by quantitative detection, realizes high-flux sample detection at the same time, conducts fast quantitative detection on the phytophthora capsici leonian and replaces the conventional identification method of isolated culture on a selective medium, and is suitable for wide popularization and application in the field of plant disease diagnosis and detection.

The invention relates to novel high-efficiency biocontrol bacterial fertilizer and a production technology thereof. The novel high-efficiency biocontrol bacterial fertilizer is named as Lvdu I. The novel high-efficiency biocontrol bacterial fertilizer is an active microbe-containing specific preparation prepared through activity recovery, propagation and expansion culture of strains, preparation of liquid bacterial fertilizer, preparation of solid bacterial fertilizer, and the like. The novel high-efficiency biocontrol bacterial fertilizer can treat multiple soil-borne diseases such as cucumber fusarium wilt, phytophthora capsici leonian, tomato bacterial wilt and the like. The novel high-efficiency biocontrol bacterial fertilizer named as Lvdu I can improve soil fertility, prolong fertilizer action time, promote growth of a few of crops, increase output, produce biocontrol effects on harmful microbes, and reduce crop diseases. The novel high-efficiency biocontrol bacterial fertilizer named as Lvdu I has the advantages of low costs, convenience of use, simple process flow, and no pollution and toxic residues.

The invention relates to a specific application of a nascent polypeptide complex protein, in particular to an application of a nascent polypeptide-associated complex (NAC) protein as a vegetable immune protein. Specifically, the application can be an application of the NAC protein in improving the gray mold resisting immunity of tomatoes, an application of the NAC protein in improving the tobacco mosaic virus resisting immunity of tomatoes, or an application of the NAC protein in improving the phytophthora capsici resisting immunity of chilli. The application process has simplicity of operation, low cost and good effect.

The invention discloses a new Pseudomonas corrugate strain (P94 GMCC No.1895), which is characterized by the following: manufacturing relative HCN to prevent bacteria, proteinase and phospholipase and IAA; inhibiting partial plant pathogenic fungus (Botrytis cinerea, Ceratocystis fimbriata, Monilinia laxa, Magnaporthe grisea, Pythium aphanidermatum and Phytophthora capsici) and partial pathogenic bacteria (Pseudomonas syringae, Acidovorax avenae and Ralstonia solanacearum); accelerating the growth of tomato and cucumber to improve production by 22%-28%.