The invention discloses a compound separated from
colletotrichum gloesporioides and a preparation method and application thereof. The molecular formula of the compound separated from the
colletotrichum gloesporioides is C15H22O3. The preparation method of the compound comprises the following steps: activating a
colletotrichum gloesporioides strain which has been cryopreserved, inoculating the activated strain to a culture medium for culturing, preparing a PDB
liquid culture medium, and sterilizing; dissolving a
bottle of
penicillin and a
bottle of
streptomycin in
sterile water, and adding the solution in a fermenting tank; inoculating the sterilized culture into the fermenting tank for fermenting; after fermenting is finished, carrying out suction
filtration on a fermented product to obtain
mycelium; extracting and concentrating to obtain a
bacteria solution extract; and carrying out
column chromatography,
elution and wet packing on a coarse extract, carrying out
elution and
column chromatography on
silica gel again, carrying out
elution with
petroleum ether-
acetone eluant again, carrying out
column chromatography, carrying out elution with
chloroform-
methanol to implement decolorization, and carrying out separation to obtain the compound which is colletotrichum gloesporioides A. The provided compound can be used for preparing anti-
senile dementia drug.