208 results about "Bacillus methylotrophicus" patented technology

The invention discloses bacillus methylotrophicus wettable powder which comprises 100 parts by mass of bacillus methylotrophicus active components, 4-10 parts by mass of a wetting agent and 4-10 parts by mass of a dispersing agent. A method for preparing the bacillus methylotrophicus wettable powder comprises the following steps: weighing the bacillus methylotrophicus active ingredients, the wetting agent and the dispersing agent according to a ratio, and uniformly mixing; and milling through a jet mill, thereby obtaining the bacillus methylotrophicus wettable powder. The wettable powder is stable in quality and convenient to preserve and use for a long time, has good effects of preventing and treating cucumber gray mold and apple rot, solves the problems such as environmental toxic and side effects and pesticide residues brought by chemical agents, has the characteristics of high efficiency, no toxicity, safety and no residue, is a safe, effective, low-residue and environment-friendly microbial pesticide and has wide application prospects in the field of microbial control.

The invention discloses a bacillus and application thereof, and belongs to the field of biological control. A bacillus strain is bacillus methylotrophicus C1, and a preservation number of the bacillus strain is GDMCC No.60046. The application includes that the bacillus can be used as a biological control strain to be applied to antagonistic crop pathogenic bacteria; the bacillus can be used as a lipopeptide and / or protein fermentation strain for inhibiting crop pathogenic bacteria; the bacillus can be used as a growth promotion strain to be applied to promoting growth of crops; the bacillus can be used as a fermentation strain for auxin. The bacillus and the application have the advantages that the strain has broad-spectrum antibacterial activity; lipopeptide substances with effects of inhibiting fusarium graminearum cannot be generated, but proteins with effects of inhibiting the fusarium graminearum can be generated; effects of promoting growth of corn can be realized by the strain C1, the root lengths and the stalk lengths of the corn of experiment groups are 3.15 and 3.05 times the root lengths and the stalk lengths of contrast groups respectively as compared with contrast, the root, stalk and seed weights of the corn of the experiment groups are 5.02, 4.33 and 1.4 times the root, stalk and seed weights of the contrast groups, and only little auxin can be generated by the strain C1.

The invention provides bacillus methylotrophicus UTM401 and applications thereof. The biological collection number of the bacillus methylotrophicus UTM401 is CGMCC No.5927. The bio-fertilizer can be prepared by inoculating the fermentation seed liquid of the strain UTM401 into an organic waste bank and fermenting. By adopting the strain UTM401 to prepare the bio-fertilizer, the cost is low, the quality is stable, and the fermentation period can be shortened; moreover, the bacteria are increased quickly again in solid material, and a stable bacterial colony system is formed with a fermentation material carrier; and high-density colonization of the plant rhizosphere can be realized, and the effects of suppressing phytopathogen and harmful microorganisms of rhizosphere, promoting the plant growth and improving the crop yield are realized. The strain UTM401 and the bio-fertilizer prepared from the fermentation organic waste thereof are green and environment-friendly and pollution-free, and have good performance and relatively high economic and social benefits.

The invention provides bacillus methylotrophicus with a collection number of CCTCC M2015297. Bacillus methylotrophicus is named bacillus methylotrophicus XK-4. The invention further provides a screening method of bacillus methylotrophicus XK-4, an application of bacillus methylotrophicus XK-4 to inhibition of sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum and a bacillus methylotrophicus XK-4 agent. Bacillus methylotrophicus XK-4 is a rape rhizosphere microorganism, is safe to human, livestock and crops, is environment-friendly, has a wider antibacterial spectrum, has better inhibitory effects on mycelial growth of common pathogenic bacteria, such as sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum, has obvious inhibitory effects on mycelial growth of sclerotinia sclerotiorum and causes mycelia deformation. The further prepared agent has obvious control effects on sclerotinia rot of colza, is short in production cycle and has a very extensive application prospect in biological control of plant diseases.

The present invention relates to a method of producing a recombinant peptide, a recombinant protein, or a product from metabolic engineering using a genetically modified methylotrophic bacterium, and more particularly to Methylobacterium extorquens ATCC 55366. The method comprises introducing am expression vector into the methylotrophic bacterium, the expression vector comprising a polynucleotide sequence, coding for a peptide or protein, or allowing for production of a product from metabolic engineering under the control of a regulated promoter. the method also comprises growing the genectically modified methylotrophic bacterium in a minimal salts medium lacking organic sugars and containing methanol. A metal ion may be used for regulating the expression of the polynucleotide sequence by the promoter.

The invention discloses bacillus methylotrophicus B18 as well as a liquid preparation prepared from the bacillus methylotrophicus B18 and application of the bacillus methylotrophicus B18. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 18, 2017 and has a preservation number of CGMCC No. 14641. The liquid preparation is prepared by taking thebacillus methylotrophicus B18 as the strain and carrying out seed cultivation and fermentation cultivation. The biocontrol bacterium-bacillus methylotrophicus B18 strain has double functions of disease prevention and growth promotion, has the characteristics of biological control and bio-fertilizer, and has remarkable effects on the control of damping-off of eucommia ulmoides and the promotion ofgrowth of the eucommia ulmoides; the bacillus methylotrophicus B18 not only can avoid the serious consequences of environmental pollution caused by chemical drugs, but also has the characteristics ofsustainability and low cost.

The invention discloses a method for preparing liquid organic fertilizer through fermentation of aquatic product waste. The method comprises main steps as follows: fresh aquatic product waste minced; the minced aquatic product waste is added to an enzymolysis tank and nutrients and water are added and mixed evenly to be used as a fermentation medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe methyl nutritional type Bacillus methylotrophicus HL-4 capable of producing protease for simultaneous enzymolysis and fermentation; a fermented broth is filtered for precipitation removal, and thus the liquid organic fertilizer can be prepared. Enzymolysis and fermentation processes can be finished in the enzymolysis tank simultaneously, equipment and operation are optimized, the production cost of the liquid organic fertilizer is significantly reduced, and the method has good application prospect.

The invention relates to the field of biopesticides and microbial fertilizers and particularly discloses bacillus methylotrophicus wettable powder containing a spore germination agent and a preparation method thereof. The wettable powder is prepared from the following raw materials in parts by weight: 40-70 parts of bacillus methylotrophicus, 20-30 parts of talcum powder serving as a padding, 1-4 parts of sodium dodecyl benzene sulfonate serving as a wetting agent, 0.2-1 part of tea saponin serving as a dispersing agent, 0.1-0.5 part of methionine and 0.1-0.5 part of 2,6-dipicolinic acid calcium salt serving as a spore germinator, 0.5-2 parts of ascorbic acid and 0.2-1 part of beta-dextrin serving as an ultraviolet protector, and 1-4 parts of maltose and 0.2-1 part of mannitol serving as a dryness protector. After the bacillus methylotrophicus wettable powder prepared by the preparation method is applied to a land for growing field crops, the spore germination speed is rapid, and the germination rate is high; and the bacillus methylotrophicus wettable powder is capable obviously improving the fertilizer efficiency and plant disease control effect, reducing the spore dosage and lowering the cost.

The invention discloses a compound fungicide FX for degrading straws. The compound fungicide FX comprises a Bacillus subtilis strain NJX501, a Bacillus subtilis strain NJX502, a Bacillus methylotrophicus strain NJXy and an Aspergillus fumigatus strain NJZ501. The compound fungicide is prepared by mixing the bacterium liquid of NJX501 with a bacterium OD value of 0.5-1, the bacterium fluid of NJX502 with a bacterium OD value of 0.5-1, the bacterium liquid of NJXy with a bacterium OD value of 0.5-1 and NJZ501 spore suspension with spore number of not less than 104 / ml in equivalent volume. The compound fungicide FX can be used for promoting the composting and decomposition of rice straws under high temperature conditions.

The invention discloses a green alga glycosyl microcapsule biological agent and its preparation method and application. Four strains Geobacillus stearothermophilus, Bacillus vallismortis, Bacillus methylotrophicus and Bacillus siamensis are mixed and compounded in proportion; and by a microencapsulation technology which is most effective and practical for protection of strain activity, the active strains are wrapped in a capsule wall substance with Enteromorpha prolifera polysaccharide being the main component. Thus, the green alga glycosyl microcapsule biological agent is prepared. In addition, the invention specifically provides a preparation method of the green alga glycosyl microcapsule biological agent. The green alga glycosyl microcapsule biological agent provided by the invention is pollution-free and can achieve the efficacy of cooperative usage of a fertilizer and a pesticide. The technology is simple and costs are low. The environment is protected. The product has high disease resistance and stress resistance. Survival rate is enhanced. The product is easy for industrial production.

The invention relates to bacillus methylotrophicus, bactericide and a preparation method and application of the bactericide.The bacillus methylotrophicus is bacillus methylotrophicus JQ-018 and is preserved in China General Microbiological Culture Collection Center on 7th December, 2015, and the preservation register number is CGMCC No:11838.The bactericide can be prepared through seed activation, seed culture and fermentation culture.The invention further provides the preparation method of the bactericide of the bacillus methylotrophicus and the application to preparation of an agent for preventing and controlling potato early blight.The bactericide has high bioactivity and good environment compatibility, is free of poisons to people and livestock and convenient to use, and can adjust plant microorganism ecological florae.