57 results about "Phanerochaete" patented technology

The invention discloses a crop straw decomposing inoculant and application in straw bioreactor and water-logged compost. The inoculant is compounded of 0.5-2 parts of phanerochaete chrysosporium, 0.5-2 parts of trichoderma aureoviride, 0.5-2 parts of bacillus subtilis and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The straw decomposing inoculant is applied to a tomato stalk bioreactor planting technology, and the average ground temperature is 18.3 DEG C and is increased by 2.2 DEG C as compared with that of contrast without use of the inoculant; the average greenhouse temperature is 14.3 DEG C and is increased by 2.0 DEG C as compared with the average greenhouse temperature of the contrast; the average CO2 concentration is 956ppm and is 2.17 times higher than that of the contrast; the harvest time is 15 days earlier than the harvest time of the contrast; the acre yield is 5881kg and is increased by 22.7 percent as compared with the acre yield of the contrast; the occurrence rate of plant diseases and insect pests is lower as compared with that of the contrast. The inoculant is applied to wheat straw retting and decomposing, is high in decomposition speed, high in compost flexibility, the straws are basically decomposed, and the fermentation time is shorter than that of an inoculant purchased in the market.

The invention discloses a vegetable waste composting bacterial agent, a using method thereof and an organic substrate prepared from the vegetable waste composting bacterial agent. The vegetable waste composting bacterial agent is prepared from the following components in parts by weight: 1-3 parts of phanerochaete chrysosporium, 1-3 parts of trichoderma harzianum, 1-3 parts of bacillus subtilis and 1-3 parts of bacillus mucilaginosus, wherein the effective viable count of the complex bacterial agent is up to 4*10<8> / g. The vegetable waste composting bacterial agent can be used for fermenting and composting vegetable wastes and resisting to cucumber fusarium wilt and has the fermentation period of 25 days for treating vegetable wastes and more than 50 days for treating uninoculated vegetable wastes. The organic substrate is prepared through fermenting by using a special process after adding 1kg of composting bacterial agent into every 5 cubic meters of vegetable wastes, wherein the vegetable wastes comprise the following components in parts by weight: 40-60 parts of eggplant straw, 20-40 parts of cucumber vine, 5-15 parts of Chinese cabbage leaves and 5-15 parts of brown coal, wherein the organic substrate can be used as a seedling substrate of tomatoes, has the emergence rate of 92% and has the same effect as a seedling substrate sold on the market.

The invention provides a complex microbial agent for stabilizing treatment of stock garbage in cities and towns as well as a preparation method and an application of the complex microbial agent. The complex microbial agent contains sporotrichum thermophile, pseudomonas alcaligenes, halobacillus sp, bacillus subtilis, pseudomonas putida, candida utilis, saccharomyces cerevisiae, phanerochaete chrysosporium and lactobacillus plantarum. Growth metabolism, degradability and activity of all microbial strains in the complex microbial agent can effectively complement and cooperate with one another, the microbial strains can grow and propagate quickly in a landfill system to promote the temperature of a garbage treatment environment to increase quickly, meanwhile, organic matter, BDM and toxic andharmful substances in a landfill can be quickly and efficiently degraded, the stabilizing time of the garbage landfill is greatly shortened, stable harmless treatment of landfill is promoted, and thecomplex microbial agent can be applied to aerobic stabilizing treatment of garbage in cities and towns in practice.

The invention discloses a Biosynthesis method of a cadmium sulfide quantum dot. The method comprises the following steps of: on the basis of taking thioacetamide as an S source, water soluble cadmium salt as a Cd source, a sulfhydryl compound as a stabilizer and a phanerochaete chrysosporium mycelium pellet as an adsorbent, adding the S source, the Cd source and the sulfhydryl compound into a culture solution which contains the phanerochaete chrysosporium mycelium pellet to carry out shake cultivation on a shaking table, and carrying out filtration, cleaning in deionized water, ultrasonication, centrifuging and purification after the cultivation to obtain the cadmium sulfide quantum dot. The method provided by the invention is simple in operation, low in cost and good in reproducibility; and the prepared cadmium sulfide quantum dot has the characteristics of low toxicity, good biocompatibility and excellent optical property.

Disclosed herein are method to modify the lignin with particular fungal species, and procedure to synthesize phenolic adhesives with the modified lignin as raw materials, and the adhesives compositions and methods for making adhesive compositions, and methods for making lingo-cellulosic composites from renewable materials. Four fungi in examples are Lenzites elegans (Spreng.) Pat. (FTK 329A), Phanerochaete cremea (Bres.) Parmasto (FTK 332A), Pycnoporellus alboluteus (Ellis & Everh.) Kotl. & Pouz. (FTK 76A) and Meruliopsis taxicola (Pers.) Bondartsev (FTK 122B). Lignin used in examples are organosolv lignin, Kraft lignin, and ammonium lignosulfonate. The present invention includes methods to (1) modify of lignin with fungi; (2) in-situ polymerize modified lignin-phenol-formaldehyde to generate bio-modified lignin-phenol-formaldehyde adhesive in liquid form, and (3) manufacture composite panels with bio-modified lignin-phenol-formaldehyde resins.

The invention discloses a vegetable waste decomposing bacterial agent, a use method thereof and an organic matrix prepared therefrom. The decomposed bacterial agent is composed of 1-3 parts of Phaneroderma chrysosporium, 1-3 parts of Trichoderma harzianum, 1-3 parts of Bacillus subtilis and 1-3 parts of Bacillus peptone-like in parts by weight; The number of effective viable bacteria reached 4×108 / g. It can ferment decomposed vegetable waste and resist cucumber wilt bacteria. The fermentation cycle of vegetable waste treatment is 25 days, while the uninoculated one needs more than 50 days. The organic matrix is ​​fermented by adding 1 kg of the decomposing bacteria agent to every 5 cubic meters of vegetable waste, and the parts by weight of the vegetable waste are as follows: 40-60 parts of eggplant stalks, 20-40 parts of cucumber seedlings , 5-15 parts of Chinese cabbage leaves and 5-15 parts of lignite, the organic substrate can be used as the seedling-raising substrate of tomato, and the emergence rate reaches 92%, reaching the effect of the seedling-raising substrate sold in the market.

The invention discloses a method for preparing modified soybean straw pig fodder and the pig fodder prepared by means of the method. The method comprises the following steps that 1, soybean straw, soybean hulls, soybean meal, soybean oil and soybean bran are placed in an ultrafine crusher to be crushed into fine powder with the particle size of 500-600 mesh; 2, a modifier is added to the fine powder in the step 1, wherein the modifier is prepared from, by weight, yeast, ceriporiopsis subvermispora, phanerochaete chrysosporium, variegated coriolus versicolor, pleurotus ostreatus, hirschioporuslacteus and coriolopsis gallica; a mixture is formed after the addition of the modifier and poured into a fermentation tank, water is added for stirring, and the moisture content of the mixture is controlled within 15%-30%; 3, after the water is added for stirring and the temperature rises to 30-40 DEG C, and fermentation is conducted for 12-20 hours; 4, the fermented material in the step 3 is taken out and placed in a granulation dryer for drying granulation, and the finished pig fodder is obtained after the granulation is completed.

The invention discloses a crop straw rot rotten bacterium and its application in straw biological reactors and fertilizers.The fungus is made of 0.5 to 2 parts of the yellow spores, 0.5 to 2 parts of yellow and green wooden mold, 0.5 to 2 copus of Bacillus of Bacillus and 0.5 to 2 parts of gluebacteria.Number 6 × 108 / g.The straw rot cooked bacterium is used in tomato straw biological reactor planting technology, with an average ground temperature of 18.3 ° C, an increase of 2.2 ° C from the control of the non -bacterial agent; the average shed temperature is 14.3 ° C, an increase of 2.0 ° C.2.17 times; the harvest period is 15 days in advance; the perurone output is 5881kg, which is 22.7%more than the control of the control. Compared with the control, there is less pests.The bacteria are used in wheat straw stacks, rot, fast decomposition, good softness, and good softening, straw is basically decomposed, and the fermentation time is shorter than the bacteria purchased by the application market.

The invention discloses a method for detection of the living cell biomass of phanerochaete chrysosporium under the heavy metal stress. The method comprises the steps as follows: 1, pellet culturing: adding the phanerochaete chrysosporium into a liquid culture medium for constant-temperature culturing, and adding heavy metal solution for stress treatment; 2, chromogenic reaction: adding the phanerochaete chrysosporium pellets obtained after heavy metal stress into sterile MTT solution and performing chromogenic reaction for 0.5-4 h at the temperature of 30-50 DEG C, and then quickly adding hydrochloric acid solution to stop the reaction, so as to obtain the mixed liquor after reaction; 3, centrifugal treatment: performing centrifugal treatment on the mixed liquor to remove the liquid supernatant and obtain the lower sediment; 4, extracting detection: adding an extracting agent into the sediment for extraction, determining the absorbance of the extract liquor, and calculating the living cell biomass of the phanerochaete chrysosporium under the heavy metal stress. According to the invention, the detection method is simple and convenient, wide in application and strong in practicability.

The invention discloses a Biosynthesis method of a cadmium sulfide quantum dot. The method comprises the following steps of: on the basis of taking thioacetamide as an S source, water soluble cadmium salt as a Cd source, a sulfhydryl compound as a stabilizer and a phanerochaete chrysosporium mycelium pellet as an adsorbent, adding the S source, the Cd source and the sulfhydryl compound into a culture solution which contains the phanerochaete chrysosporium mycelium pellet to carry out shake cultivation on a shaking table, and carrying out filtration, cleaning in deionized water, ultrasonication, centrifuging and purification after the cultivation to obtain the cadmium sulfide quantum dot. The method provided by the invention is simple in operation, low in cost and good in reproducibility; and the prepared cadmium sulfide quantum dot has the characteristics of low toxicity, good biocompatibility and excellent optical property.

The invention discloses a method for cultivating summer squashes in a greenhouse in a high-altitude area. The method specifically comprises the following steps: white spirit yellow water and an organic fertilizer are applied into soil, and rare earth and carbon-soluble organic fertilizer are added as a base fertilizer; during field planting, straws and wine-making waste distillers' grains are deeply buried between every two adjacent ridges, phanerochaete chrysosporium and bacillus amyloliquefaciens are added, and the mixture is covered with soil; in the seedling stage, gamma-PGA polyglutamic acid begins to be subjected to drip irrigation, meanwhile, bacillus mucilaginosus liquid is subjected to drip irrigation once, and amino acid selenium fertilizer is sprayed on leaf surfaces; calcium-boron fertilizer is subjected to drip irrigation in the early stage of flowering and pollination, organic silicon fertilizer is sprayed on leaf surfaces, and compound sodium nitrophenolate is applied along with water in the flowering stage; when the fruits begin to expand, trichoderma viride 3.2942 spore bacterial liquid and amino acid water-soluble nutrient fertilizer are subjected to drip irrigation and mixed with monopotassium phosphate for use at the same time, drip irrigation is conducted once every 15-20 days, and drip irrigation is conducted 2-4 times; after the first batch of summer squashes are picked, topdressing of bacillus mucilaginosus liquid is performed along with water; in addition, the monopotassium phosphate, the carbon-soluble organic fertilizer and the amino acid water-soluble nutrient fertilizer are subjected to drip irrigation along with water, and drip irrigation is performed once a month. The method effectively improves the acre yield of the summer squashes.