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Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent

A technology of vegetable waste and decomposing bacteria agent, which is applied in the application field of agricultural microorganisms in the treatment of vegetable waste, to achieve the effect of increasing yield, improving crop quality, and increasing the number of beneficial microorganisms

Active Publication Date: 2014-08-06
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few special decomposing bacteria agents for treating vegetable waste in China.

Method used

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  • Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent
  • Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent
  • Vegetable waste composting bacterial agent, using method thereof and organic substrate prepared from vegetable waste composting bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of media and strains

[0039] 1. Preparation of culture medium

[0040] 1. Potato and dextrose agar medium (PDA)

[0041] Take 200 grams of peeled potatoes, cut them into small pieces, add 1000ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000ml, add 20 grams of glucose, 15 grams of agar, dissolve and subpackage, and sterilize for 30 minutes in 15 pounds , the pH is natural, and no agar is added when preparing the liquid medium.

[0042] 2. Nutrient gravy agar

[0043] It consists of 5g of peptone, 5g of sodium chloride, 3g of beef extract and 1000ml of distilled water. pH7.0 (add 15-20g of agar to the solid medium).

[0044] 3. Potassium bacteria medium

[0045] Yeast Extract 0.4g, Mannitol 10.0g, K 2 HPO 4 0.5g, MgSO 4 7H 2 O 0.2g, FeCl 3 0.005g, CaCO 3 1.0g, 1000.0ml distilled water, pH7.0-7.2, add 15g agar when preparing solid medium.

[0046] 4. Solid fermentation medium

[0047] 80g wheat bran,...

Embodiment 2

[0061] Composition of compound bacterial agent

[0062] A vegetable waste decomposing bacteria agent is prepared as follows (by weight):

[0063] Table 1 Composition of vegetable waste decomposing bacteria agent

[0064] name Phaneroderma chrysosporium Trichoderma harzianum Bacillus subtilis Bacillus peptiformis （1） 1.0 2.0 1.0 3.0 （2） 2.0 3.0 2.5 2.0 （3） 2.5 2.5 1.5 1.5 （4） 3.0 1.5 2.0 1.0

[0065] The number of effective viable bacteria is 4×10 8 piece / g;

[0066] Table 2 Proportion of raw materials for organic matrix production (by weight percentage)

[0067] name Eggplant straw (%) Cucumber seedlings (%) Chinese cabbage seedlings (%) lignite(%) （1） 60 30 10 10 （2） 45 40 15 5 （3） 50 30 5 15 （4） 40 20 10 5

Embodiment 3

[0069] Mutual antagonism experiment between strains

[0070] The activated Phanerolea chrysosporium, Trichoderma harzianum, Bacillus subtilis and Bacillus peptone-like were subjected to mutual antagonism experiments among strains, and they were combined in pairs, divided into 12 combinations in total, and each combination was repeated three times.

[0071] The steel ring method was used to carry out the antibacterial test, and the strain was cultured in the corresponding medium for 3-5 days (190r / min, 28°C) to make a fermentation broth. Take 0.1mL of the fermentation broth of one of the bacteria (A) and spread it on a PDA plate, then place two pre-sterilized steel circles on the solid medium of each plate, and then add 0.25mL to each steel circle For the fermentation broth of any bacteria (B) other than bacteria A, each treatment was repeated three times, and the plate only inoculated with bacteria A was used as a control. Incubate at 28°C and observe the antibacterial situ...

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Abstract

The invention discloses a vegetable waste composting bacterial agent, a using method thereof and an organic substrate prepared from the vegetable waste composting bacterial agent. The vegetable waste composting bacterial agent is prepared from the following components in parts by weight: 1-3 parts of phanerochaete chrysosporium, 1-3 parts of trichoderma harzianum, 1-3 parts of bacillus subtilis and 1-3 parts of bacillus mucilaginosus, wherein the effective viable count of the complex bacterial agent is up to 4*10<8> / g. The vegetable waste composting bacterial agent can be used for fermenting and composting vegetable wastes and resisting to cucumber fusarium wilt and has the fermentation period of 25 days for treating vegetable wastes and more than 50 days for treating uninoculated vegetable wastes. The organic substrate is prepared through fermenting by using a special process after adding 1kg of composting bacterial agent into every 5 cubic meters of vegetable wastes, wherein the vegetable wastes comprise the following components in parts by weight: 40-60 parts of eggplant straw, 20-40 parts of cucumber vine, 5-15 parts of Chinese cabbage leaves and 5-15 parts of brown coal, wherein the organic substrate can be used as a seedling substrate of tomatoes, has the emergence rate of 92% and has the same effect as a seedling substrate sold on the market.

Description

technical field [0001] The invention belongs to the field of application of agricultural microorganisms in processing vegetable waste, and relates to a vegetable waste decomposing bacterial agent, a use method thereof, and an organic matrix prepared therefrom. Background technique [0002] According to statistics, by the end of 2010, Tianjin had accumulatively built 355,000 mu of planting facilities, and the city's protected agriculture area reached 550,000 mu. During the "Twelfth Five-Year Plan" period, Tianjin's protected agriculture will reach 1 million mu. The large-scale increase of facility agriculture has greatly increased the output of vegetables, melons and fruits in Tianjin, but also produced a large amount of vegetable waste. Among them, tomato produces 40-60 tons of fresh waste per hectare, cauliflower produces 80-110 tons of fresh waste per hectare, and cucumber produces 50-66 tons of fresh waste per hectare. Vegetable waste has high water content, is easy to r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/00C05F11/08C05F11/02A01P3/00A01P21/00C12R1/645C12R1/885C12R1/125C12R1/07
CPCY02W30/40
Inventor 何宗均赵琳娜梁海恬田阳
Owner 天津市农业科学院
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