1288 results about "Enrichment culture" patented technology

The invention discloses a large-scaled culture method of high-concentration nitrosobacteria and application thereof. The method comprises the following steps of: (1) adding an enrichment culture solution with initial ammonia nitrogen mass concentration of 30-500mg/L in a reaction device with functions of stirring, heating and aeration; preparing activated sludge of a waste water treatment plant in activated sludge of 0.5-20% for primary inoculation to conduct the enrichment culture of the nitrosobacteria by 2-6d as a culture cycle, wherein extra ammonium salts and a growth promoter are added during the enrichment culture of the nitrosobacteria; (2) ending the culture when the concentration of NO2-N in the solution is accumulated to reach 70-95% of the concentration of total nitrogen; adding a flocculating agent for flocculation and sedimentation; and allowing to stand still to remove supernate, and then adding the enrichment culture solution to start the culture of the second cycle; and (3) continuously conducting 3-8 cycles of the enrichment culture to obtain the high-concentration nitrosobacteria. By adopting the nitrosobacteria to strengthen the bi-nitrification treatment of ammonia nitrogen contained waste water, the method has the advantages of high stress resistance, low cost and high efficiency and is suitable for the complicated treatment of ammonia nitrogen contained industrial waste water.

The invention discloses a preparation method and an application of immobilized bacteria for improving the denitrification efficiency of a constructed wetland. 1. The preparation steps are as follows: 1) screening and culturing aerobic denitrification strains which are screened out of the constructed wetland in a thermotank; 2) selecting aerobic denitrification bacteria in slope conservation, inoculating into a sterile enrichment culture medium and aerating to get bacterial suspension; 3) using sodium alginate-polyvinyl alcohol to embed so as to get the immobilized aerobic denitrification bacteria; and 4) taking pellets of the aerobic denitrification bacteria out of a refrigerator, washing with double distilled water or physiological saline, soaking in the physiological saline, and aerating to activate the immobilized pellets. 2. An application of the immobilized bacteria in the constructed wetland is as follows: filling the immobilized pellets into a simulation column of the constructed wetland for performing waste water denitrification treatment. The preparation method of the immobilized bacteria is simple, the production cost is low, the application in the industrial waste water treatment is easy, the denitrification efficiency is high, the accumulation amount of nitrite nitrogen is low, and the service life is long.

The invention relates to a method for preparing an enzyme and bacterium composite agent for treating sewage and sludge, which comprises the following steps: (1) preparing bacillus subtilis powder; (2) preparing bacterial solution of photosynthetic bacteria; (3) obtaining indigenous bacteria by collection, culture and enrichment culture; (4) performing domestication culture of working bacteria, namely adding indigenous bacteria and a composite culture medium according to different sludge loads, adding bacillus subtilis enzyme powder in an amount which is 0.1 to 0.4 percent based on total weight and bacterial solution of photosynthetic bacteria in an amount which is 1 to 3 percent based on the total weight, performing tests under different loads and different organic matter concentration, determining an optimal parameter, and repeating for 3 times; and (5) performing domestication and amplification culture of the working bacterium composite agent, and obtaining the domesticated working bacterium composite agent in which the viable count per milliliter is cfu 10<7> to 10<9>. The enzyme and bacterium composite agent prepared by the method can treat sewage and sludge efficiency, simplyand conveniently, has very high adaptability to any sewage environment, and has a guiding and coordinating effect on beneficial decomposing bacteria in local environments.

The invention discloses an enrichment culture method of salt-tolerant nitrifying bacterium communities, which comprises the steps that: activated sludge is inoculated, and the culture mode is the sequencing batch type enrichment culture; the enrichment culture is carried out in simulated wastewater, the main ingredient of nutritive salt of the enrichment culture is inorganic salt, the substrate is externally added ammonium salt, and a carbon source is inorganic carbonate; the pH value in an aeration tank in the enrichment culture process is regulated and controlled through sodium carbonate and sodium bicarbonate, and the dissolved oxygen value is regulated through the aeration quantity; a method for gradually increasing the salinity is adopted for domesticating the nitrifying bacterium communities; and when no filamentous bacterium is observed in the sludge and the number and the variety of protozoans are few, the enrichment culture of the nitrifying bacterium communities is finished. The enrichment culture method has the advantages that the available matrix range is wide, the culture is easy, the concentration of the cultured salt-tolerant nitrifying bacterium communities is high, the activity is good, the biochemical treatment removal rate of high-salt ammonia-nitrogen wastewater can be obviously improved, the enrichment culture method is suitable for the treatment of various ammonia-nitrogen industrial wastewater and is suitable for the scale production, the wastewater treatment cost is reduced, and good economic benefits and environmental benefits are realized.

A rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua relates to the tissue culture technology of plants. The formulas of culture mediums are as follows: an induction culture medium includes MS, 1.0 mg/L of 6-BA, and 0.5 mg/L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg/L of 6-BA, 0.5 mg/L of NAA, and 60 g/L of sugar, and a proliferation culture medium for the bud body parts include MS, 1.0 mg/L of 6-BA, 0.5 mg/L of 2,4-D, and 60 g/L of sugar, a proliferation and seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg/L of 6-BA, 0.5 mg/L of NAA, 0 to 50 mg/L of paclobutrazol, and 60 g/L of sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg/L of 6-BA, 0.5 mg/L of NAA, and 0.5% of activated charcoal. The method comprises the steps as follows: the surface layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.

The invention relates to a tissue culture and rapid propagation method of acer palmatum. The method comprises the following steps of: after a series of sterilization processes, carrying out isolated culture on acer palmatum stems by adopting NN69 as a basic culture medium; and establishing a rapid propagation system of the acer palmatum by utilizing hormone regulation and control to successfully culture an integrated rooting regeneration plant with a transplant survival rate of about 90 percent, wherein a starting culture medium for inoculating a primary generation comprises the NN69, 0.1mg/L of NAA (Naphthylacetic Acid), 0.3mg/L of TDZ (Thidiazuron) and 30g/L of sucrose with a pH value of 5.8; an enrichment culture medium comprises the NN69, 30g/L of sucrose, 0.1mg/L of NAA and 0.8mg/L of KT-30; and a rooting culture medium comprises 1/2 NN69, 0.2mg/L of NAA, 20g/L of sucrose and 0.2g/L of active carbon. The method overcomes the defects of long conventional seedling culturing period of the acer palmatum, low cuttage propagation coefficient, and the like and realizes the industrial production of acer palmatum nursery stocks.

The invention provides a preparation method of a kimchi fermenting agent and a fermenting bacterium powder. The preparation method comprises the steps: activating strains of lactobacillus plantarum, lactobacillus brevis, lactobacillus pentosus and lactobacillus paracasei; preparing an enrichment medium; carrying out enrichment culture on the activated strains; centrifugally concentrating the kimchi fermenting agent; preparing a protective agent; and carrying out spray drying on the kimchi fermenting agent and the protective agent. The kimchi fermenting agent and the fermenting bacterium powder prepared by using the method can be used for effectively reducing nitrite in kimchi; a vegetable generally used as kimchi is selected as the enrichment medium, so that not only can lactic acid bacteria be effectively proliferated, but also the enrichment medium has the characteristics of natural green and no addition of other chemical reagents; a spray drying method is used as a drying way for preparing the fermenting bacterium powder, so that the production cost can be effectively reduced.

The invention discloses a method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation. The method comprises the following steps: adopting fiber and sponge combined ball-type packings which are independently developed as carriers to perform screening, placing high effective strain which is obtained through screening in culture solution 'HS enrichment medium' to perform enrichment culture, then inoculating the strain which is through the enrichment culture in 'HSK culture medium' to perform expending cultivation and prepare biological microbial inoculum, placing packings in the microbial inoculum to perform biofilm formation and obtain biological immobilized packings; connecting the prepared biological immobilized packings with a string to fix the packings around the laboratory patented device water-lifting aerator of the invention and adopting water-lifting aeration-situ bio-contact oxidation to perform bioremediation to the micro-scale polluted reservoir water. The method of the invention can be used to effectively reduce the concentrations of COD, N, P and other pollutants in reservoir water and especially effectively lower the total nitrogen content of the micro-scale polluted reservoir water so as to fundamentally resolve the pollution problem of reservoir water, inhibit the growth of algae and control water eutrophication.

The invention discloses an immobilized nitrobacteria enrichment culture method and device in a sewage treatment process. A sequencing-batch biomembrane activated sludge process is utilized to perform enrichment culture on nitrobacteria: dewatered sludge is inoculated in an immobilized nitrobacteria culture device, and an enrichment culture solution is added; and in the nitrobacteria enrichment culture process, the biomembrane ammonia nitrogen load is gradually enhanced, and the pH value is controlled. The enrichment culture solution is formed by mixing NH4HCO3 and pretreated municipal sewage, the ammonia nitrogen concentrations are respectively 100, 300, 500, 700 and 1000 mg/L, and the COD (chemical oxygen demand) concentration is 30-60 mg/L. By controlling the immobilized nitrobacteria culture device, the DO in the reaction mixture is 2-6 mg/L, the pH value is 6.5-8.0, the biofilm-formation acclimatization culture temperature is 10-14 DEG C, and the enrichment purification culture temperature is 15-25 DEG C. The device can be used for culturing nitrobacteria at low temperature, and the nitrobacteria content is up to 40-45%. The immobilized nitrobacteria have high low-temperature nitration resistance, and can treat the wastewater of which the ammonia nitrogen concentration is up to 1000 mg/L, so that the ammonia nitrogen in the wastewater is lowered to 1.0 mg/L or below.

The invention discloses a composite bacterial agent for efficiently degrading kitchen garbage, and a preparation method and application thereof. The efficiently degrading kitchen garbage, and preparation method and application thereof consists of a starch-degrading strain, an oil-degrading strain, a protein-degrading strain, a cellulose-degrading strain and a vector; and the preparation method comprises the following steps of: performing enrichment culture on each single strain, screening, and preparing the vector; and mixing a high-quality composite bacterial agent with the vector. The synergism of various microorganisms in the natural world is fully utilized, a microorganism-based control method is established, and the composite bacterial agent is utilized for coupled fermentation of the kitchen garbage to accelerate the biogas fermentation progress, improve the traditional fermentation process and improve the biogas yield.

The invention provides a method and a device for quickly enriching ammonia oxidizing bacteria. The ammonia oxidizing bacteria are enriched by a sequencing batch reactor, a method of gradually increasing ammonia nitrogen concentration in a culture solution and a pH process controlling method; and the adopted culture solution for enriching comprises the inorganic salt used as a main component, and a trace element culture solution, a buffer solution and ammonia carbonate; and the concentration of dissolved oxygen in the culture process is maintained more than 5mg/L by a process controlling device, pH is maintained 7.0 to 8.5, and temperature is maintained 20 to 25 DEG C. By the scheme, the growth of heterotrophic bacteria in activated sludge can be inhibited obviously, and the enriched ammonia oxidizing bacteria are 60 to 65 percent of the total number of microorganism bacteria in the activated sludge, and are tolerant to high ammonia nitrogen concentration; and finally high-ammonia-nitrogen wastewater of which the concentration can reach 500mg/L can be treated, and high-concentration ammonia nitrogen in the wastewater can be reduced to less than 1.0mg/L.

The invention discloses a porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and a preparation method thereof. The preparation method comprises the following steps of: a, respectively carrying out enrichment culture on a porcine streptococcus strain, a haemophilus parasuis strain and a haemophilus parasuis strain to obtain a porcine streptococcus strain bacterial solution, a haemophilus parasuis strain bacterial solution and a haemophilus parasuis strain bacterial solution; b, respectively adding a formaldehyde solution into the porcine streptococcus strain bacterial solution, the haemophilus parasuis strain bacterial solution and the haemophilus parasuis strain bacterial solution, and inactivating; c, mixing the collected porcine streptococcus strain bacterial solution, the haemophilus parasuis strain bacterial solution and the haemophilus parasuis strain bacterial solution, adding Tween-80 for preparing a water phase, preparing white oil, Span-80 and aluminium stearate into an oil phase, mixing the water phase with the oil phase to prepare a uniform emulsion, i.e. an oil emulsion inactivating vaccine; and 4, sub-packaging the oil emulsion inactivating vaccine. The porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine can effectively prevent the porcine streptococcus disease and haemophilus parasuis disease, does not have hidden danger of scattering viruses and is safe and reliable; and the immunization is realized by one vaccine, thus the cost is reduced.

The invention provides a culture method of salt-tolerant anaerobic ammonium oxidation bacteria, which comprises the following steps of: A, enrichment culture: performing enrichment culture on the strain to obtain anaerobic ammonium oxidation bacteria; and B, bacteria acclimation culture: gradually increasing the concentration of salt in the culture solution to perform salt tolerance acclimation culture of the anaerobic ammonium oxidation bacteria subjected to the enrichment culture in the step A, wherein the acclimation period of the bacterial at each salt concentration is 15-30 days; and the step A and step B both adopt sequencing batch culture, and the dissolved oxygen value in the culture solution is 0-0.5mg/L. The bacteria cultured by the method provided by the invention are applied to high-salt ammonia nitrogen wastewater, and the total nitrogen removal rate is high. By adopting the method provided by the invention to culture the salt-tolerant anaerobic ammonium oxidation bacteria, the range of available substrates is wide, and the culture is easy to implement; and additional organic carbon source is not required in the bacteria culture and application process, investment is greatly saved, and the wastewater treatment cost is reduced. The bacteria culture method provided by the invention can be applied to large-scale production, and has good economic and environmental benefits.

The invention discloses an immobilized microbial preparation which is prepared through the following steps: S1, fermentation culturing strain: respectively inoculating bacillus subtilis, bacillus megatherium, nitrobacter and nitrosomonas in an enrichment medium to be cultured; S2, mixing thallus to obtain mixed thallus liquor; S3, immobilizing PVA-sodium alginate, namely uniformly mixing PVA gel with the mixed thallus liquor, dripping the mixture into a boric acid solution containing CaCl2 while continuously stirring at a low speed, immobilizing and crosslinking the mixture, taking out the mixture, washing the mixture through deionized water, and freeze drying the mixture to obtain the immobilized microbial preparation. The invention further discloses a method of treating meat product processing waste water by utilizing the immobilized microbial preparation. The immobilized microbial preparation disclosed by the invention has the beneficial effects that the microorganism categories are various, various pollutants in the waste water can be degraded, the degrading speed is fast and the capability is strong; the immobilized microbial preparation is suitable for changes of water quality and pH; the sludge yield is small, the products are liable to separate, the stability is good, the technique operation is simple, and the production cost is low.