102 results about "MIXED FLORA" patented technology

The invention discloses gloconacetobacter xylinum and a method for fermenting high-yield bacterial celluloses through mixed flora. The gloconacetobacter xylinum HNX01 with the preservation number of CGMCC No. 5173 was preserved in the CGMCC (China General Microbiological Culture Collection Center) on Aug. 19, 2011. According to the method for fermenting high-yield bacterial celluloses through the mixed flora, the mixed flora includes gloconacetobacter xylinum CGMCC No. 5173, acetobacter sp. CGMCC No. 6641 and lactobacillus sp. CGMCC No. 5172. The yield of the obtained bacterial celluloses is more than 26.5 g/L (dry basis weight). The method provides sufficient nutrition for the growth of the gloconacetobacter xylinum and fully utilizes a culture medium to ferment high-yield bacterial celluloses with compact and uniform structures.

The invention provides russula vinosa Lindbl yield increasing fungi and a using method thereof, which belong to the technical field of russula vinosa Lindbl yield increase and solve the problems of low yield, high price, low quality and the like of wild russula vinosa Lindbl of the prior art. The russula vinosa Lindbl yield increasing fungi of the invention are a mixed flora of locally common ectomycorrhizal fungi and comprise Boletus brunneissimus Chiu, R. albida Peck, Boletus luridus and amanita yellow corner. Mycelia of various fungi of the russula vinosa Lindbl yield increasing fungi are cultured by a shaker culture method respectively; the mycelia are separate and used to prepare mycelium mixtures at a concentration of 50mg/L respectively; the mixtures are mixed uniformed in the same amount before inoculation to form the russula vinosa Lindbl yield increasing fungi; and on a woodland wherein russula vinosa Lindbl grows, the russula vinosa Lindbl yield increasing fungi are inoculated in dent pits by a root cutting and mycorrhization technique. In the method, ectomycorrhizal fungi flora is used to increase the biomass of thin roots of arbor, so the fruiting capacity, yield and quality of wild russula vinosa Lindbl are improved obviously and remarkable economic benefits are created. Thus, the method is suitable to be promoted and used in large areas.

The invention provides a culture medium. The culture medium is prepared from the following raw materials in parts by weight: 30 to 50 parts of rural soil, 20 to 40 parts of garden wastes, 10 to 25 parts of traditional Chinese medicine residues and 1 to 5 parts of mixed flora. The culture medium belongs to the technical field of artificial cultivation of plants. The culture medium provided by the invention can be used for improving the porosity and permeability of the soil and has good ventilation and water drainage performances; the volume weight of the soil is reduced and the content of organic matters in the soil is increased, so that the soil keeps continuous fertility and the disease resisting performance of the afforestation plants can also be enhanced.

The invention relates to the field of microbial power generation, in particular to a mixed flora and a purpose thereof, a microbial electrogenesis system containing the mixed flora, and a microbial fuel cell. Through construction of an engineered mixed flora, the characteristic of definite division of labor is realized; zymophyte 1 provides carrier riboflavin of electron transfer for the system; and zymophyte 2 converts most common cheap glucose into a small molecule acid and provides the small molecule acid for electrochemically active microorganisms, so that the spectrum of carbon sources of the electrochemically active microorganisms is expanded. According to the mixed flora and the purpose thereof, the microbial electrogenesis system containing the mixed flora and the microbial fuel cell, two zymophytes with different purposes are constructed by performing methods, such as genetic engineering alteration, on type strains of escherichia coli or bacillus subtilis-protokaryotic gram negative bacteria and gram-positive bacteria; and therefore, the spectrum of carbon sources of the microbial fuel cell is expanded and the electron transfer efficiency of the electrochemically active microorganisms is enhanced. A stable, reasonable and efficient functional mixed flora compound electrogenesis system is constructed from three important angles of a material flow, an energy flow and an information flow.

The invention relates to the field of microbial electricity generation and especially relates to a microbial electricity generation system comprising a mixed bacterial flora and a microbial fuel cell. In the invention, an engineered mixed bacterial flora is constructed, wherein each of the bacteria has definite effects. A first fermentation bacteria provides a carrier, riboflavin, for electron transfer for the system; a second fermentation bacteria converts the most common and low-cost glucose into small-molecular acids for the electricity generating bacteria, thereby enlarging carbon source spectrum of the electricity generating bacteria. In the invention, escherichia coli or bacillus subtilis, which are model strains of prokaryotes Gram negative bacteria and Gram positive bacteria, are selected, through genetic engineering modification and the like, two fermentation bacteria having different applications are constructed, so that the carbon source spectrum of the microbial fuel cell is enlarged and electron transfer efficiency of the electricity generating bacteria is increased. Meanwhile, from three important angles, including material flow, energy flow and information flow, a stable, reasonable and high-effective functional mixed bacterial symbiotic electricity generation system is constructed.

The invention discloses a microbial mixed flora for promoting growth of plant stems and belongs to the technical field of agricultural microorganisms. The microbial mixed flora is composed of azotobacter beijerimckii, Anhui rhizobium and paenibacillus polymyxa. The microbial mixed flora is prepared by adopting a following method. The method comprises the steps that the azotobacter beijerimckii, Anhui rhizobium and paenibacillus polymyxa are inoculated to an LB culture medium separately, shaking culture at 30 DEG C is conducted until the concentration of the flora O.D600 is about 2.0, and through mixing according to the volume ratio of 1:1:1, the microbial mixed flora is obtained. The microbial mixed flora has an obvious effect of promoting growth of the plant stems and increasing the yield of crops and has wide application prospects in agricultural production.

A method for preparing a composite matrix from pig's excrement in order to culture cyclamen includes such steps as mixing said pig's excrement with a mixed flora (OL) containing multiple kinds of microbes, fermenting, and proportionally mixing it with peat, vermiculite and pearlite.

The invention discloses a disease-resistant growth-promoting mixed microbial flora, and belongs to the technical field of microorganisms. The flora provided by the invention comprises streptomyces corchorusii, bacillus paralicheniformis and cellulose nodule bacteria; wherein the microbial flora is prepared by the following method: respectively inoculating streptomyces corchorusii, bacillus paralicheniformis and cellulose rhizobium strains on an LB solid culture medium for activation, respectively inoculating the strains into an LB liquid culture medium according to the inoculum size of 2% for amplification culture to respectively obtain 1.5 * 10<10> CFU/mL fermentation broth, and mixing the fermentation broth according to the proportion. According to the invention, strains with biocontrol and growth promotion functions are screened to form a mixed flora, synergistic interaction is realized, cucumber root knot nematode diseases are effectively prevented and controlled, and the quality and yield of cucumbers are effectively improved.

The invention discloses a method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment. The kitchen waste is taken as a raw material for producing a high-value-added product, so that recycling of biomass is achieved when the waste is fully utilized. By combining with a hydro-thermal pretreatment technology, the lactic acid fermentation yield of the kitchen waste is increased by common action of microorganisms and complex enzyme. Simple and available microbe bacteria are selected to prepare mixed flora, and meanwhile, a common enzyme is selected to prepare the complex enzyme, so that efficient fermentation to produce lactic acid is achieved. The method is simple and convenient in fermentation technology, good in fermentation effect and especially applicable to industrial application.

The invention discloses an in-situ restoring method for a nitrophenol isomeride polluted environment by utilizing a microorganism mixed flora and an application. The in-situ restoring method comprises the following steps: A) constructing the microorganism mixed flora: a) preparing an inorganic salt culture medium, b) performing induction culture on strains and c) collecting thallus; B) constructing different micro environmental models: a) constructing a soil micro environmental model and b) preparing a biological reaction vessel; and C) placing a soil sample at 30 DEG C, placing the biological reaction vessel at room temperature, and sampling and detecting the change in pollutant concentration at regular time. According to the method provided by the invention, the in-situ restoring of thesoil containing 40ppm per milligram dry weight nitrophenol can be realized; the biological restoring for three nitrophenols in the 50 milligram per liter biological reaction vessel can be realized; multiple inoculated bacteria can continuously and stably exist in the soil and the reaction vessel, thereby proving that the in-situ restoring method can be applied to the environmental treatment of soil and water containing nitrophenol isomeride pollution; and a new idea for treating the environmental pollutant is supplied.

The invention relates to a native bacteria collection method, which specifically comprises: selecting cooked rice as a culture medium, and placing into a wood box as a bacteria collecting box; selecting a place having termite formicary as a bacteria collection point, and burying the bacterial collection box into the termite formicary to collect native bacteria strains; and adding the rice having the strains to brown sugar having the same amount as the rice, placing the obtained mixture into a pottery jar, fermenting to obtain original strains, and continuously carrying out fermentation enlargement on the original strains and a certain amount of a culture medium comprising wheat bran, rice bran, corn powder and brown sugar to obtain production strains. According to the present invention, the termite formicary is adopted as the bacteria collection point and the termite mainly adopts rotted wood as the food, such that a large number of mixed floras capable of decomposing cellulose, lignin, and a variety of beneficial microorganisms exist in the termite formicary so as to collect a lot of the high-quality native bacteria strains in a short term; and the bacteria collection box is buried in the soil, such that the impurity bacteria in the collected strain is less, and the strain quality is excellent.

The invention discloses a cotton fabric desizing method based on compounding of crude enzyme and amylase produced by mixed flora and belongs to the technical field of textile. Small-molecule polyalcohol is added to serve as an inductive agent, the PVA degrading enzyme activity of crude enzyme produced by mixed flora is remarkably improved, the use amount of the needed crude enzyme in the process of degrading polyvinyl alcohol slurry is effectively reduced, and culture time and culture cost of preparation of the crude enzyme are saved; meanwhile, no expensive commercial PVA degrading enzyme needs to be purchased, and the method is suitable for industrial application. According to the method, the problems of flocculation and difficult separation of PVA degrading enzyme caused by a high-polymerization polyvinyl alcohol inductive agent are avoided.

The invention discloses a method for rapidly enriching a functional flora without production of foreign odor substances in pit mud and an application of the functional flora, belonging to the technical field of brewing microorganisms. According to the method, tested and screened aged pit mud with high quality is used as seed mud, and selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained flora is a caproic acid-producing flora taking a clostridium rumen flora as a main body, and the flora does not generate a mud odorsubstance, namely 4-methylphenol. The mixed flora obtained by using the method disclosed by the invention can be used for a solid fermented grain fermentation process of a pit-mud-free system; the content of beneficial flavor substances of fermented grains can be remarkably increased; the generation of bad flavor substances in the pit mud is avoided; thus, the quality of raw wine is improved. Themixed flora can also be used in a culture process of artificial pit mud and a daily pit mud maintenance process, so the caproic acid-producing main flora in a pit can be increased; pit mud aging is promoted; pit mud aging and hardening are prevented; meanwhile, generation of the mud odor substance, namely the 4-methylphenol is effectively reduced.

The invention relates to the field of microbes and discloses a pair of PCR primers for amplification of streptomyces DNA, wherein the sequence of a forward primer is shown in SEQ ID No.1, and the sequence of a reverse primer is shown in SEQ ID No.2. The invention further provides a method and a kit for detecting streptomyces. The primers provided by the invention are suitable for amplification of purified streptomyces DNA as well as for amplification of purified streptomyces DNA from mixed flora, are safe, accurate, quick and stable in amplification of streptomyces, and have high specificity and sensitivity.