Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

103 results about "MIXED FLORA" patented technology

The significance of urine culture with mixed flora. Siegman-Igra Y. Urine cultures that contain more than one organism are usually considered contaminated. The frequency with which such growth truly represents mixed infection is unknown. Surprisingly few studies have evaluated the clinical significance of polymicrobial growth from urine.

Composite mineral-leaching bacteria colony and use of same in biological metallurgy

ActiveCN101538540AImprove efficiencyBroaden the scope of ore processingBacteriaMicroorganism based processesIonLaboratory culture
The invention relates to a composite mineral-leaching bacteria colony and use of the same in biological metallurgy. The composite mineral-leaching bacteria colony has a preserving number of CGMCC No.2395 in the Common Micro-organism Center of China General Microbiological Culture Collection Center. The composite mineral-leaching bacteria colony has strong oxidative activity within a temperature range from 30 to 52 DEG C as well as a working pH value of between 0.8 and 2.25 and an arsenic ion tolerance of 0 to 20 g / L. The composite mineral-leaching bacteria colony is used for extracting and recovering gold or copper, and greatly expands the use range of biological metallurgy in gold mines. Meanwhile, due to the wide temperature adaptation range, the composite mineral-leaching bacteria colony improves the oxidation speed and reduces production cost. Therefore, the composite mineral-leaching bacteria colony has a significant application prospect in biological metallurgy industry.
Owner:CHINA NAT GOLD GROUP CORP TECH CENT +1

Gloconacetobacter xylinum and method for fermenting high-yield bacterial celluloses through mixed flora

The invention discloses gloconacetobacter xylinum and a method for fermenting high-yield bacterial celluloses through mixed flora. The gloconacetobacter xylinum HNX01 with the preservation number of CGMCC No. 5173 was preserved in the CGMCC (China General Microbiological Culture Collection Center) on Aug. 19, 2011. According to the method for fermenting high-yield bacterial celluloses through the mixed flora, the mixed flora includes gloconacetobacter xylinum CGMCC No. 5173, acetobacter sp. CGMCC No. 6641 and lactobacillus sp. CGMCC No. 5172. The yield of the obtained bacterial celluloses is more than 26.5 g / L (dry basis weight). The method provides sufficient nutrition for the growth of the gloconacetobacter xylinum and fully utilizes a culture medium to ferment high-yield bacterial celluloses with compact and uniform structures.
Owner:SOUTH CHINA UNIV OF TECH

Method for producing feed from detoxified corn residue after producing alcohol by mixed bacterial micro-ecological formulation

The invention discloses a method for producing feedstuff by utilization of mixed flora microecological preparation for detoxication of corn alcohol residue, wherein candida utili, Bacillus subtilis and rhamnose lactobacillus are subjected to high-density fermentation, so that the viable count of the candida utili is more than 4.0X10<8>CFU / g, the viable count of the Bacillus subtilis is more than 3.8X10<8>CFU / g, and the viable count of the rhamnose lactobacillus is more than 3.0X10<8>CFU / g; and the preparation is directly mixed with the corn residue after production of alcohol or other toxic feedstuff polluted by mildew according to the proportion of between 5 and 10 grams per kilogram, and the mixture is sprayed with sterile water to maintain a humidity of more than 40 percent, reacts for 2 to 10 hours at a temperature of between 30 and 35 DEG C, is dried at a temperature of between 45 and 50 DEG C, and can degrade ZEA in the corn alcohol residue or the other toxic feedstuff, wherein the detoxication rate reaches more than 94 percent, so that the corn alcohol residue or the other toxic feedstuff becomes safe animal feedstuff.
Owner:SOUTH CHINA UNIV OF TECH

Aspergillus flavus strain and mixed flora not producing aflatoxin and application thereof

The invention discloses an Aspergillus flavus strain and mixed flora not producing aflatoxin and application thereof. The strain is Aspergillus flavus AF052 with a preservation number of CGMCC NO.9858 and a preservation data of November 20th, 2014, and Aspergillus flavus AF053 with a preservation number of CGMCC NO.9859, and a preservation date of November 20th, 2014. All the strain and flora can effectively inhibit the formation of aflatoxin in corn and peanut, and improve the quality of agricultural products. In addition, A051, AF052 and AF053 can also secrete cellulose. When the corn and straw treated by biocontrol flora prepared from the Aspergillus flavus are used as animal feed, the carried biocontrol bacteria can produce cellulose to degrade cellulose and improve the utilization efficiency of animal husbandry on feed.
Owner:ZHEJIANG UNIV

Russula vinosa Lindbl yield increasing fungi and using method thereof

InactiveCN101690454APromote recoveryIncreased fine root biomassHorticultureFertilizer mixturesFloraEconomic benefits
The invention provides russula vinosa Lindbl yield increasing fungi and a using method thereof, which belong to the technical field of russula vinosa Lindbl yield increase and solve the problems of low yield, high price, low quality and the like of wild russula vinosa Lindbl of the prior art. The russula vinosa Lindbl yield increasing fungi of the invention are a mixed flora of locally common ectomycorrhizal fungi and comprise Boletus brunneissimus Chiu, R. albida Peck, Boletus luridus and amanita yellow corner. Mycelia of various fungi of the russula vinosa Lindbl yield increasing fungi are cultured by a shaker culture method respectively; the mycelia are separate and used to prepare mycelium mixtures at a concentration of 50mg / L respectively; the mixtures are mixed uniformed in the same amount before inoculation to form the russula vinosa Lindbl yield increasing fungi; and on a woodland wherein russula vinosa Lindbl grows, the russula vinosa Lindbl yield increasing fungi are inoculated in dent pits by a root cutting and mycorrhization technique. In the method, ectomycorrhizal fungi flora is used to increase the biomass of thin roots of arbor, so the fruiting capacity, yield and quality of wild russula vinosa Lindbl are improved obviously and remarkable economic benefits are created. Thus, the method is suitable to be promoted and used in large areas.
Owner:INST OF EDIBLE FUNGI FUJIAN ACAD OF AGRI SCI

Method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using aerobic dynamic drainage method

The invention discloses a method for quickly acclimatizing active sludge mixed flora to prepare polyhydroxyalkanoate (PHA) by using an aerobic dynamic drainage method and relates to a method for preparing the PHA. The invention solves the problems that the acclimatization time for synthesizing PHA through active sludge mixed flora is long, a reactor works unstably and the synthesized PHA is low in content in the prior art. The method comprises the following steps: 1, active sludge is put into a sequencing batch reactor (SBR); 2, 6-12 hours are taken as a cycle of operation, in the cycle of operation, two-time water introduction, precipitation and drainage are carried out, the drainage rate is 1:1, during the first water introduction, a carbon source and various nutrients are introduced, and during the second water introduction, only the supplement of nitrogen nutrient is carried out; 3, 2-4 cycles are operated every day, the sludge detention time is 1-10 days and sludge-water mixed liquid is collected; 4, batch experiments are carried out, half supernatant is discharged, equivalent carbon source is supplemented, aeration, precipitation and drainage are carried out, and the process is repeated for 6 to 8 times. The invention belongs to the field of biodegradable plastic synthesis.
Owner:HARBIN INST OF TECH

Culture medium and cultivation method of roof afforestation plants

The invention provides a culture medium. The culture medium is prepared from the following raw materials in parts by weight: 30 to 50 parts of rural soil, 20 to 40 parts of garden wastes, 10 to 25 parts of traditional Chinese medicine residues and 1 to 5 parts of mixed flora. The culture medium belongs to the technical field of artificial cultivation of plants. The culture medium provided by the invention can be used for improving the porosity and permeability of the soil and has good ventilation and water drainage performances; the volume weight of the soil is reduced and the content of organic matters in the soil is increased, so that the soil keeps continuous fertility and the disease resisting performance of the afforestation plants can also be enhanced.
Owner:GUANGDONG DONGLEE ENVIRONMENT CO LTD

Microbial flora continuous screening device

The invention discloses a microbial flora continuous screening device which comprises a culture medium storer, a feed liquid reaction chamber and a microbial flora collecting chamber. The bottom of the culture medium storer is connected to the feed liquid reaction chamber through a first hose, the bottom of the feed liquid reaction chamber is connected to the microbial flora collecting chamber through a second hose, and a drain hose is arranged at the bottom of the microbial flora collecting chamber. Based on a microbial continuo's culture theory, microbial screening is located in a dynamic state, nutritional substances required by microorganisms can be immediately and continuously supplemented, and part of harmful metabolites can be immediately discharged to promote growth and reproduction of microorganisms; by taking nutritional substances such as the culture medium as a food of microorganisms, related microbial flora is promoted to form a food chain, and finally, target degrading flora is quickly screened, so that the demand on screening the microbial mixed flora in industrial scaled production is met.
Owner:INNER MONGOLIA UNIV OF SCI & TECH

Mixed flora and purpose thereof, microbial electrogenesis system containing mixed flora, and microbial fuel cell

The invention relates to the field of microbial power generation, in particular to a mixed flora and a purpose thereof, a microbial electrogenesis system containing the mixed flora, and a microbial fuel cell. Through construction of an engineered mixed flora, the characteristic of definite division of labor is realized; zymophyte 1 provides carrier riboflavin of electron transfer for the system; and zymophyte 2 converts most common cheap glucose into a small molecule acid and provides the small molecule acid for electrochemically active microorganisms, so that the spectrum of carbon sources of the electrochemically active microorganisms is expanded. According to the mixed flora and the purpose thereof, the microbial electrogenesis system containing the mixed flora and the microbial fuel cell, two zymophytes with different purposes are constructed by performing methods, such as genetic engineering alteration, on type strains of escherichia coli or bacillus subtilis-protokaryotic gram negative bacteria and gram-positive bacteria; and therefore, the spectrum of carbon sources of the microbial fuel cell is expanded and the electron transfer efficiency of the electrochemically active microorganisms is enhanced. A stable, reasonable and efficient functional mixed flora compound electrogenesis system is constructed from three important angles of a material flow, an energy flow and an information flow.
Owner:TIANJIN UNIV

Microbial electricity generation system comprising mixed bacterial flora and microbial fuel cell

The invention relates to the field of microbial electricity generation and especially relates to a microbial electricity generation system comprising a mixed bacterial flora and a microbial fuel cell. In the invention, an engineered mixed bacterial flora is constructed, wherein each of the bacteria has definite effects. A first fermentation bacteria provides a carrier, riboflavin, for electron transfer for the system; a second fermentation bacteria converts the most common and low-cost glucose into small-molecular acids for the electricity generating bacteria, thereby enlarging carbon source spectrum of the electricity generating bacteria. In the invention, escherichia coli or bacillus subtilis, which are model strains of prokaryotes Gram negative bacteria and Gram positive bacteria, are selected, through genetic engineering modification and the like, two fermentation bacteria having different applications are constructed, so that the carbon source spectrum of the microbial fuel cell is enlarged and electron transfer efficiency of the electricity generating bacteria is increased. Meanwhile, from three important angles, including material flow, energy flow and information flow, a stable, reasonable and high-effective functional mixed bacterial symbiotic electricity generation system is constructed.
Owner:TIANJIN UNIV

Microbial mixed flora for promoting growth of plant stems

The invention discloses a microbial mixed flora for promoting growth of plant stems and belongs to the technical field of agricultural microorganisms. The microbial mixed flora is composed of azotobacter beijerimckii, Anhui rhizobium and paenibacillus polymyxa. The microbial mixed flora is prepared by adopting a following method. The method comprises the steps that the azotobacter beijerimckii, Anhui rhizobium and paenibacillus polymyxa are inoculated to an LB culture medium separately, shaking culture at 30 DEG C is conducted until the concentration of the flora O.D600 is about 2.0, and through mixing according to the volume ratio of 1:1:1, the microbial mixed flora is obtained. The microbial mixed flora has an obvious effect of promoting growth of the plant stems and increasing the yield of crops and has wide application prospects in agricultural production.
Owner:LINYI UNIVERSITY

Method for cultivating synchronous nitrogen and sulfur removal mixed flora

The invention discloses a method for cultivating a synchronous nitrogen and sulfur removal mixed flora. The method comprises the steps that anaerobic ammonium oxidation granular sludge is inoculated in a reactor, simulated wastewater containing ammonia nitrogen, nitrate nitrogen, sulfide, inorganic salt, trace elements and sodium bicarbonate is taken as influent water, the temperature is 30-36 DEGC, and the reactor runs to be stable under the anaerobic condition of controlling the hydraulic retention time to be constant; the cultivation of the synchronous nitrogen and sulfur removal mixed flora is completed by means of a method of increasing the concentration of the sulfide in the influent water gradually, decreasing the concentration of the ammonia nitrogen and nitrite gradually, then increasing the concentration of the sulfide gradually and finally shortening the hydraulic retention time in sequence. According to the method, the anaerobic ammonium oxidation sludge is adopted as a single inoculum, the synchronous nitrogen and sulfur removal mixed flora is cultivated by means of the step-by-step domestication method, and the treatment of the wastewater simultaneously containing the ammonia nitrogen, nitrate nitrogen and sulfide in chemical plants and the like is facilitated.
Owner:浙江国泉生态环境有限公司

Efficient mixed flora for treating sludge

The invention discloses efficient mixed flora for treating sludge which comprises comamonas testosteroni, pseudomonas alcaligenes, stenotrophomonas rhizophila, pseudomonas pseudoalcaligenes and azospirillum. The mixed flora can effectively decompose the sludge.
Owner:YUNNAN SHENGQING ENVIRONMENTAL PROTECTION TECH CO LTD

Probiotic bacterium preparation for chicken and preparing method of preparation

The invention discloses a probiotic bacterium preparation for chicken and a preparing method of the preparation. The probiotic bacterium preparation is a mixed flora mainly composed of bacillus subtilis, beer yeast and lactobacillus casei; the probiotic bacterium preparation comprises, by weight, 50%-70% of the bacillus subtilis, 10%-30% of the beer yeast and 20%-40% of the lactobacillus casei; and the preparing method of the micro-ecology probiotic bacterium preparation includes the following steps that culture excitation, first-stage liquid enlarging cultivation, second-stage liquid enlarging cultivation and fixation to rice chaff are carried out; drying and smashing are carried out; and the bacillus subtilis, the beer yeast and the lactobacillus casei are mixed according to the proportion that the bacillus subtilis ranges from 50% to 70%, the beer yeast ranges from 10% to 30%, and the lactobacillus casei ranges from 20% to 40%. The micro-ecology dominant flora is formed in the intestines and stomach of the chicken through the probiotic bacterium preparation, nutrition conversion can be effectively improved, growth of the chicken is promoted, and the feed conversion ratio is reduced.
Owner:SRICK TIANJIN BIO TECH

Mixed microorganism flora for promoting growth of plant roots

The invention discloses a mixed microorganism flora for promoting growth of plant roots, and belongs to the technical field of agricultural microorganisms. The mixed flora is composed of bacillus megatherium, pseudomonas brassicacearum and azospirillum brasilense; the mixed microorganism flora is prepared by adopting a following method. The preparation method comprises the steps that the bacillus megatherium, the pseudomonas brassicacearum and the azospirillum brasilense are separately inoculated in an LB culture medium, vibration culture is conducted at 30 DEG C until the bacterial concentration O.D600 is about 2.0, then the bacillus megatherium, the pseudomonas brassicacearum and the azospirillum brasilense are mixed according to the volume ratio of 1:1:1, and the mixed microorganism flora is obtained. The mixed microorganism flora is superior to a single bacterial strain, all the bacterial strains have a synergistic effect, a significant effect of promoting growth of plant root systems is achieved, and the mixed microorganism flora quite highly adapts to actual soil rhizosphere complex environments.
Owner:LINYI UNIVERSITY

Method for preparing composite cultivation medium for growing cyclamen by using pig dung as raw material

A method for preparing a composite matrix from pig's excrement in order to culture cyclamen includes such steps as mixing said pig's excrement with a mixed flora (OL) containing multiple kinds of microbes, fermenting, and proportionally mixing it with peat, vermiculite and pearlite.
Owner:NANJING UNIV

Mixed flora and application thereof in iron removal and whitening for kaolin

The invention discloses mixed flora and application thereof in iron removal and whitening for kaolin. The mixed flora is prepared from the following components: iron-reducing bacteria RBFL, iron-reducing bacteria FeRB-FL1404, iron-reducing bacteria FL-HI, thermoanaerobacter ethanolicus, achromobacter, Geobacter bremensis Straub and Buchholz-Cleven; due to a synergistic effect of microbial strainsin the mixed flora, the iron-removing effect of the microbes for the kaolin can be improved. The bacterial liquid obtained by culturing the mixed flora is used for reducing Fe2O3 in the kaolin, and insoluble Fe2O3 is reduced into water-soluble Fe<2+> which can be removed by solid-liquid separation, so that the content of Fe2O3 in the kaolin can be reduced and the whiteness of the kaolin can be improved. The mixed flora has the advantages that the cost is low, the environmental-friendly effect is achieved, the kaolin ore with original low mining value and low grade can be mined, the service life of the mine can be prolonged, and the high quality of the low-grade kaolin can be realized.
Owner:FUJIAN NORMAL UNIV

Disease-resistant growth-promoting mixed microbial flora

The invention discloses a disease-resistant growth-promoting mixed microbial flora, and belongs to the technical field of microorganisms. The flora provided by the invention comprises streptomyces corchorusii, bacillus paralicheniformis and cellulose nodule bacteria; wherein the microbial flora is prepared by the following method: respectively inoculating streptomyces corchorusii, bacillus paralicheniformis and cellulose rhizobium strains on an LB solid culture medium for activation, respectively inoculating the strains into an LB liquid culture medium according to the inoculum size of 2% for amplification culture to respectively obtain 1.5 * 10<10> CFU / mL fermentation broth, and mixing the fermentation broth according to the proportion. According to the invention, strains with biocontrol and growth promotion functions are screened to form a mixed flora, synergistic interaction is realized, cucumber root knot nematode diseases are effectively prevented and controlled, and the quality and yield of cucumbers are effectively improved.
Owner:成都云图控股股份有限公司

Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment

The invention discloses a method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment. The kitchen waste is taken as a raw material for producing a high-value-added product, so that recycling of biomass is achieved when the waste is fully utilized. By combining with a hydro-thermal pretreatment technology, the lactic acid fermentation yield of the kitchen waste is increased by common action of microorganisms and complex enzyme. Simple and available microbe bacteria are selected to prepare mixed flora, and meanwhile, a common enzyme is selected to prepare the complex enzyme, so that efficient fermentation to produce lactic acid is achieved. The method is simple and convenient in fermentation technology, good in fermentation effect and especially applicable to industrial application.
Owner:SOUTH CHINA AGRI UNIV

Crab apple enzyme and preparation method thereof

The invention relates to a preparation method of a crab apple enzyme. The method comprises the following steps: preparing crab apple pulp after pretreatment of crab apples, and subjecting the crab apple pulp to enzymolysis by a pectase so as to obtain enzymatic hydrolysate; adding fermentation auxiliary materials into the enzymatic hydrolysate, adjusting pH value to 5-8, and conducting sterilization; and inoculating the sterilized product with mixed flora for synchronous fermentation, carrying out low-temperature and after-ripening conversion, conducting filtration, collecting supernatant, andconducting blending with functional sugar. The crab apple enzyme is prepared by a biological enzymolysis and probiotics fermentation conversion process so as to fully retain and release nutritional ingredients in the crab apples including active probiotics and active secondary metabolite produced by the metabolism of the probiotics, and the mouth feel of the enzyme is adjusted by the functional sugar, so that the enzyme gets tastier and mer. The crab apple enzyme does not contain any chemical auxiliary material, has rich natural active ingredients and strong antioxidant capacity, and is a premium enzyme product capable of regulating the intestinal balance of the human body, removing cholesterol and free radicals in the body and delaying aging.
Owner:QILU UNIV OF TECH

Traditional Chinese medicinal compound probiotic biological preparation for laying hens and preparation method thereof

The present invention discloses a traditional Chinese medicinal compound probiotic biological preparation for laying hens and a preparation method thereof. The traditional Chinese medicinal compound probiotic biological preparation is prepared by fermenting traditional Chinese medicinal mixed powder using compound probiotics. The traditional Chinese medicinal mixed powder is prepared from the following raw materials in parts by weight: 10-20 parts of radix astragali, 10-20 parts of herba gynostemmatis pentaphylli, 20-30 parts of radix isatidis, 5-8 parts of angelica sinensis, 5-8 parts of rhizoma atractylodis macrocephalae, 5-8 parts of rhizoma chuanxiong, 10-15 parts of radix dichroa, 10-15 parts of herba agrimoniae, 20-30 parts of ligustrum lucidum leaves, 5-8 parts of pouzolzia zeylanica, 5-8 parts of concretio silicea bambusae, 7-9 parts of semen vaccariae, 5-8 parts of radix pulsatillae and 10-20 parts of cinnamomum camphora leaves. The probiotics are mixed flora consisting of lactic acid bacteria, bacillus subtilis, rhodopseudomonas palustris and beer yeasts. The traditional Chinese medicinal compound probiotic biological preparation for the laying hens combines the traditional Chinese medicines and compound probiotics, can strengthen the gastrointestinal function and ovarian function of the laying hens, raises the immunity of the hens, promotes the growth of the young hens, reduces the morbidity and raises the egg production of the laying hens.
Owner:安徽奥力欣生物科技有限公司

In-situ microorganism restoring method for nitrophenol isomeride polluted environment and application

The invention discloses an in-situ restoring method for a nitrophenol isomeride polluted environment by utilizing a microorganism mixed flora and an application. The in-situ restoring method comprises the following steps: A) constructing the microorganism mixed flora: a) preparing an inorganic salt culture medium, b) performing induction culture on strains and c) collecting thallus; B) constructing different micro environmental models: a) constructing a soil micro environmental model and b) preparing a biological reaction vessel; and C) placing a soil sample at 30 DEG C, placing the biological reaction vessel at room temperature, and sampling and detecting the change in pollutant concentration at regular time. According to the method provided by the invention, the in-situ restoring of thesoil containing 40ppm per milligram dry weight nitrophenol can be realized; the biological restoring for three nitrophenols in the 50 milligram per liter biological reaction vessel can be realized; multiple inoculated bacteria can continuously and stably exist in the soil and the reaction vessel, thereby proving that the in-situ restoring method can be applied to the environmental treatment of soil and water containing nitrophenol isomeride pollution; and a new idea for treating the environmental pollutant is supplied.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI

Application of microbial preparation for efficiently converting heavy metal cadmium in polluted soil

The invention discloses an application of a microbial preparation for efficiently converting heavy metal cadmium in polluted soil. The microbial preparation is prepared by performing enriched cultivation on the following two mixed floras: (1) candida mycoderma bacteria, aspergillus monicus, rhodosporidium toruloides and cryptococcus which form a flora 1; and (2) acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and thermos ferric bacteria which form a flora 2. By using the microbial preparation to treat the cadmium polluted soil, the metal cadmium is converted from being indissolvable into being soluble and the removal rate is 70-82%. The microbial preparation disclosed by the invention has the advantages of being high in conversion efficiency, short in period, low in cost, free of secondary pollution, easy to popularize and the like.
Owner:HUNAN AGRI BIOTECH RES CENT +1

Native bacteria collection method

The invention relates to a native bacteria collection method, which specifically comprises: selecting cooked rice as a culture medium, and placing into a wood box as a bacteria collecting box; selecting a place having termite formicary as a bacteria collection point, and burying the bacterial collection box into the termite formicary to collect native bacteria strains; and adding the rice having the strains to brown sugar having the same amount as the rice, placing the obtained mixture into a pottery jar, fermenting to obtain original strains, and continuously carrying out fermentation enlargement on the original strains and a certain amount of a culture medium comprising wheat bran, rice bran, corn powder and brown sugar to obtain production strains. According to the present invention, the termite formicary is adopted as the bacteria collection point and the termite mainly adopts rotted wood as the food, such that a large number of mixed floras capable of decomposing cellulose, lignin, and a variety of beneficial microorganisms exist in the termite formicary so as to collect a lot of the high-quality native bacteria strains in a short term; and the bacteria collection box is buried in the soil, such that the impurity bacteria in the collected strain is less, and the strain quality is excellent.
Owner:陆良珠

Cotton fabric desizing method based on compounding of crude enzyme and amylase produced by mixed flora

ActiveCN105369583AImprove the activity of PVA degrading enzymeReduce dosageBacteriaHydrolasesFlocculationPolyol
The invention discloses a cotton fabric desizing method based on compounding of crude enzyme and amylase produced by mixed flora and belongs to the technical field of textile. Small-molecule polyalcohol is added to serve as an inductive agent, the PVA degrading enzyme activity of crude enzyme produced by mixed flora is remarkably improved, the use amount of the needed crude enzyme in the process of degrading polyvinyl alcohol slurry is effectively reduced, and culture time and culture cost of preparation of the crude enzyme are saved; meanwhile, no expensive commercial PVA degrading enzyme needs to be purchased, and the method is suitable for industrial application. According to the method, the problems of flocculation and difficult separation of PVA degrading enzyme caused by a high-polymerization polyvinyl alcohol inductive agent are avoided.
Owner:JIANGNAN UNIV

Method for rapidly enriching functional flora without production of foreign odor substances in pit mud and application of functional flora

The invention discloses a method for rapidly enriching a functional flora without production of foreign odor substances in pit mud and an application of the functional flora, belonging to the technical field of brewing microorganisms. According to the method, tested and screened aged pit mud with high quality is used as seed mud, and selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained flora is a caproic acid-producing flora taking a clostridium rumen flora as a main body, and the flora does not generate a mud odorsubstance, namely 4-methylphenol. The mixed flora obtained by using the method disclosed by the invention can be used for a solid fermented grain fermentation process of a pit-mud-free system; the content of beneficial flavor substances of fermented grains can be remarkably increased; the generation of bad flavor substances in the pit mud is avoided; thus, the quality of raw wine is improved. Themixed flora can also be used in a culture process of artificial pit mud and a daily pit mud maintenance process, so the caproic acid-producing main flora in a pit can be increased; pit mud aging is promoted; pit mud aging and hardening are prevented; meanwhile, generation of the mud odor substance, namely the 4-methylphenol is effectively reduced.
Owner:JIANGNAN UNIV +1

Screening technique of anaerobic degraded petroleum hydrocarbon methanogens

The invention relates to a screening technique of anaerobic degraded petroleum hydrocarbon methanogens. The method includes steps of taking oil field produced liquid and oil-containing sludge as samples, and performing anaerobic culture on flora therein; applying petroleum hydrocarbon as a carbon source of flora in the absence of carbon source and further cultivating and screening flora using the petroleum hydrocarbon as the carbon source. Meanwhile, the method uses the oil field produced liquid and oil-containing sludge as samples simultaneously to obtain the degraded petroleum hydrocarbon mixed flora with stronger adaptation, and respectively screen samples of which gas yields are more than 0.4 mu mol / d and 1.15 mu mol / d according to different properties of the oil field produced liquid and flora in oil-containing sludge; the samples are further mixed, so that the mixture for degrading petroleum hydrocarbon mixed flora is obtained.
Owner:LINYI UNIVERSITY

Method for in-situ revealing anaerobic iron ammonia oxidizing bacteria in river and lake sediment by DNA stable isotope probe

The invention discloses a method for in-situ revealing anaerobic iron ammonia oxidizing bacteria in river and lake sediment by a DNA stable isotope probe. The method comprises the following steps of (1) collecting a nitrogen-polluted river and lake sediment sample; (2) carrying out <13>C- and <15>N- isotope incubation experiments; (3) separating heavy-layer DNA by adopting a cesium chloride density gradient solution ultra-high-speed centrifugation method; and (4) carrying out DNA marking degree evaluation and flora structure analysis. The invention provides a method for analyzing and identifying the anaerobic iron ammonia oxidizing bacteria in a non-culture mixed flora in a natural water body environment, which is of great significance for researching and understanding a nitrogen circulation process in which iron reduction participates and developing an autotrophic nitrogen transformation technology.
Owner:GUANGDONG UNIV OF TECH

PCR primers for amplification of Streptomyces, and method and kit for detecting Streptomyces

The invention relates to the field of microbes and discloses a pair of PCR primers for amplification of streptomyces DNA, wherein the sequence of a forward primer is shown in SEQ ID No.1, and the sequence of a reverse primer is shown in SEQ ID No.2. The invention further provides a method and a kit for detecting streptomyces. The primers provided by the invention are suitable for amplification of purified streptomyces DNA as well as for amplification of purified streptomyces DNA from mixed flora, are safe, accurate, quick and stable in amplification of streptomyces, and have high specificity and sensitivity.
Owner:ADVANCED ENERGY & ENVIRONMENTAL TECH INC

Aeromonas bestiarum having PHAs synthesizing performance

The invention relates to aeromonas bestiarum having PHAs synthesizing performance and belongs to the field of environmental microorganisms. The aeromonas bestiarum strain is classified and named as aeromonas bestiarum and has the collection number of CGMCC No. 10332. The strain is obtained by screening from an acclimated activated sludge mixed flora system and can be applied in preparation of polyhydroxy alkanoates. By selecting fermentation synthesis conditions and adopting sodium acetate as a carbon source, the total amount of PHAs synthesized by the strain accounts for 63.21% of the dry weight of thalli.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products