Method for rapidly enriching functional flora without production of foreign odor substances in pit mud and application of functional flora

Abstract

The invention discloses a method for rapidly enriching a functional flora without production of foreign odor substances in pit mud and an application of the functional flora, belonging to the technical field of brewing microorganisms. According to the method, tested and screened aged pit mud with high quality is used as seed mud, and selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained flora is a caproic acid-producing flora taking a clostridium rumen flora as a main body, and the flora does not generate a mud odorsubstance, namely 4-methylphenol. The mixed flora obtained by using the method disclosed by the invention can be used for a solid fermented grain fermentation process of a pit-mud-free system; the content of beneficial flavor substances of fermented grains can be remarkably increased; the generation of bad flavor substances in the pit mud is avoided; thus, the quality of raw wine is improved. Themixed flora can also be used in a culture process of artificial pit mud and a daily pit mud maintenance process, so the caproic acid-producing main flora in a pit can be increased; pit mud aging is promoted; pit mud aging and hardening are prevented; meanwhile, generation of the mud odor substance, namely the 4-methylphenol is effectively reduced.

Description

technical field [0001] The invention discloses a method for rapidly enriching functional flora of pit mud without producing odorous substances and an application thereof, belonging to the technical field of brewing microorganisms. Background technique [0002] In the production of Luzhou-flavor liquor, the cellar pool is the basis for the brewing of Luzhou-flavor liquor, and the quality of pit mud determines the quality of liquor to a large extent. In recent years, studies on pit mud with different quality differences have shown that the microbial groups of better quality pit mud are mainly concentrated in Clostridia, Bacteroidetes and methanogens, and Caproiciproducens in Clostridia ) accounted for a relatively high relative abundance, generally reaching about 20%. These caproic acid-producing bacteria are the dominant bacteria in pit mud that utilize lactic acid metabolism to generate caproic acid. At the same time, the physical and chemical test analysis of the pit mud s...

AI Technical Summary

Problems solved by technology

However, the isolation process of these strains is relatively complicated, requiring repeated dilution and coating isolation

In addition, these single strains are easy to contaminate miscellaneous bacteria in the process of anaerobic culture, and in the application of brewing system, single strains have relatively strong competition with the complex native microorganisms in pit mud, and may not really play a role in some cases

Although the patent CN 105385644 uses a mixed bacterial agent for degrading lactic acid and synthesizing caproic acid for cellar maintenance, the mixed bacterial agent is composed of Clostridium tyrobutyricum, Clostridium sporogenes and Clostridium krusei. The relative abundance in pit mud is relatively low, that is, the mixed bacterial agent provided is not the main body of pit mud that degrades lactic acid to generate caproic acid.

[0006] During the long-term use of pit mud, on the one hand, it contributes a large amount of beneficial flavor substances, such as caproic acid, ethyl caproate, etc., but the mud odor produced by it significantly reduces the quality of Luzhou-flavor original wine

Images