76results about How to "Small amount of inoculum" patented technology

The invention relates to a new clean production technique of lactic acid; the concrete steps of the technique comprise: firstly, saccharification solution made from substances containing sugar is added with nutritious substances and lactobacillus for fermentation; then a porous membrane is used for fining fermentation solution, and the lactobacillus in the solution is retained and returned to a fermentation unit for reuse; nanofiltration is adopted to carry out discoloration and purification to the penetrate solution of the porous membrane, and concentrated solution after nanofiltration is returned to the fermentation unit after being processed by a ceramic membrane sterilization system; the penetrate solution after nanofiltration enters a bipolar membrane electrodialysis system for preparing the lactic acid and liquid caustic produced simultaneously is returned for use; and finally, vacuum distillation is adopted to concentrate lactic acid solution, thus obtaining the lactic acid which is a finished product. The production technique is characterized by little pollution and low material consumption, realizes bacteria recycling fermentation, saves inoculation quantity, lowers fermentation cost as well as greatly reduces fermentation water consumption and improves the quality of lactic acid products.

The invention relates to a method for preparation of oligopeptides by solid state fermentation cake, and relates to the field of biological engineering. One or more cakes as a raw material is crushed for pretreatment, a sweep frequency ultrasonic assisted cultured microbial fermentation liquid seed is inoculated, the cake moisture content is adjusted to 50%-60% (w / w), field assisted fermentation is performed for 60-80 hours at 30 to 35 DEG C in a multi-layer conveyer belt type fermentation bed, after the fermentation is completed, water is added for extracting the oligopeptides, and a cake oligopeptide product is obtained by centrifugal slag removal, decolorization, drying and other steps of extracting solution. The method can significantly reduce the cost of preparation of the oligopeptides by the solid state fermentation cake, and improve the production efficiency. The oligopeptides prepared by the method can be used for human health and nutrition products, animal daily diets and the like.

The invention discloses a method for producing animal rabies viruses and animal rabies vaccines on a large scale. By using a bioreactor, the animal rabies viruses and the animal rabies vaccines are produced by a cell micro-carrier suspension culturing system on a large scale by the following steps of: inoculating cells for preparing the vaccine into a carrier tank containing culture solution and micro-carriers to attach the cells to the micro-carriers; growing the cells on the micro-carriers until the concentration of culture solution is 5 to 20 times the inoculation concentration under a proper culturing environment; preparing virus suspension from the rabies virus to adsorb the virus suspension to the cells; replacing cell maintenance culture solution to culture the virus under the proper culturing environment; continuously culturing for 3 to 5 days and harvesting the virus solution for the first time, wherein the solution replacement ratio is 50 percent by using a semi-continuous process; continuously culturing for 9 to 11 days and harvesting and replacing the solution once every 24 hours; and mixing the harvested virus solution and the virus solution of the bioreactor, repeatedly performing freeze-thawing twice at the constant temperature of -20 DEG C, inactivating, purifying and adding adjuvants to prepare the rabies vaccine. The method has the advantages of large production scale, high single-batch yield and relatively low production cost.

The invention discloses a preparation method of an aluminum-containing adjuvant hepatitis B vaccine, belonging to the biotechnology field. The preparation method is characterized in that an aluminum adjuvant Al(OH)3 is produced by an on-line reaction, i.e. after a phosphate buffer solution (PBS), a KAl(SO4)2 solution and a hepatitis B surface antigen stock solution are mixed, an NaOH solution is added to the mixed solution, an Al(OH)3 adjuvant is continuously produced, and simultaneously, hepatitis B surface antigens are continuously coated and adsorbed; and the process is called 'in-situ adsorption'. In the invention, the Al(OH)3 adjuvant is produced by an in-situ reaction to greatly improve the adsorption rate of the hepatitis B surface antigens, thereby improving the immunogenicity of the antigens, being capable of more effectively causing organisms to generate an immune response, and producing more protective antibodies. The practice proves that the aluminum adjuvant hepatitis B vaccine produced by the method disclosed by the invention has the advantages of small inoculation amount, few adverse responses, high antibody positive conversion rate and the like, and can induce high-level antibody response after being immunized. Simultaneously, the processing steps are also simplified, and the production cost is greatly lowered.

The invention discloses a method for preparing a classical swine fever (CSF) vaccine by using a cell microcarrier suspension culture system, which comprises the following steps of: (1) inoculating cells for preparing the vaccine to a carrier tank containing culture solution and a microcarrier, and uniformly mixing the cells and the microcarrier to make the cells attached to the microcarrier; (2) when the concentration after cell proliferation is 5 to 40 times of the initial inoculation concentration, inoculating CSF virus (lapinized virus) to the cells according to multiplicity of infection (M.O.I.) of the virus of 0.01-1 and reproducing the virus; and (3) mixing prepared virus liquid, adding an appropriate freeze-drying protective agent, fully and uniformly mixing, quantitatively packaging, and freeze-drying to obtain the CSF vaccine. The CSF vaccine produced by the method has the advantages of high density of cultured cells, continuous culture, high yield of the virus, high immune effect, high safety, complete immune protection on attack of violent CSF, and the like.

The invention discloses a method for preparing virus of porcine reproductive and respiratory syndrome on a large scale. In the method, the virus of the porcine reproductive and respiratory syndrome is prepared in a cell microcarrier suspension culture system by a bioreactor. The method comprises the following steps of: inoculating host cells for preparing the virus to a carrier tank containing culture solution and a microcarrier, and mixing the cells and the microcarrier uniformly to ensure that the cells are attached to the microcarrier; providing sufficient nutrients and appropriate gas environment for the cells under the appropriate culture environment to ensure that the cells are grown until the cells are in an amount which are 10 to 20 times of the inoculation concentration on the microcarrier; preparing virus suspension from the virus of the porcine reproductive and respiratory syndrome by using cell maintenance culture solution to ensure that the suspension is adsorbed to the cells; culturing the virus under the appropriate culture environment; culturing continuously for 2 to 3 days to obtain virus solution; and after the virus solution passes inspection, performing freeze thawing on the virus solution twice at the temperature of -20 DEG C, and inactivating and purifying to prepare an inactivated vaccine of the porcine reproductive and respiratory syndrome or adding a freeze-drying protective agent for freeze drying to prepare a live vaccine of the porcine reproductive and respiratory syndrome. The method has large production scale, high yield of single batch and low production cost.

The invention discloses a method for preparing 7alpha,15alpha-dyhydroxyldehydroepiandrosterone by utilizing flax colletotrichum mould. The method comprises the following steps: (1) performing spore culture, namely taking flax colletotrichum mould ST-1 as a production strain, performing slant culture, thereby obtaining mature spores; (2) performing mycelium culture, namely performing mycelium culture in a mycelium culture medium in which the spores obtained in the step (1) are inoculated, thereby obtaining the mycelium culture liquid; (3) performing biotransformation, namely adding carboxymethyl oleoyl-chitosan and DHEA into the mycelium culture liquid in the step (1), wherein the final concentration of the DHEA is 10.5-12g / L, the weight ratio of the carboxymethyl oleoyl-chitosan to DHEA is (2-3):1, transforming under the stirring and rotating speed of 200-220 revolutions per minute for 36-60 hours, thereby obtaining the transformation liquid; and (4) extracting the product, extracting and concentrating the transformation liquid by using ethyl acetate, thereby obtaining the 7alpha,15alpha-dyhydroxyldehydroepiandrosterone.