860 results about "Mixed culture" patented technology

A method for inexpensive and efficient PHA biosynthesis includes operating a sequencing bioreactor in alternating phases of nutrient deprivation and carbon feedstock deprivation to select for robust PHA-producing microbes. Preferably, the bioreactor is operated in a non-sterile manner with mixed cultures of methanotrophs. The method also preferably uses periodic biomass-wasting (PHA harvesting) at the end of the carbon feed phase, gradually lengthening the time period of carbon deprivation phase to create a penalty for rapid PHA degradation and incentive for PHA accumulation. Also, bacterial enrichment cultures may be introduced periodically. The PHA-accumulating bacteria are preferably grown on common anaerobic degradation products, specifically volatile fatty acids, such as acetate and propionate, and methane gas. The PHA has useful applications in bioplastics and other products.

The invention discloses fish feed for mixed culture and a preparation method of the fish feed. The feed comprises one or more of the following ingredients: soya bean meal, rapeseed cakes, rice bran meal, salt, liquid vitamin C, betaine and traditional Chinese medicine additives, and the preparation method comprises the steps of smashing, blending and pelletizing. The fish feed and the preparationmethod have the benefits that on the premise that the yield of grass carps is slightly increased, the yields of breams, crucian carps, variegated carps and silver carps are significantly increased; the utilization efficiency of the feed is effectively improved; the cost of the feed is saved; and the economic benefits of feeding freshwater fish are improved.

The invention discloses a Chinese drugs multi-bacterium species mixture solid fermenting method and its application. It is made up from crushing the Chinese drugs, filtering, adjusting water content and pH value, adding bean pulp, bran, and inorganic salt, sterilization, cooling and adding bacterium species fermenting liquid to take ferment, and drying. The invention could be used to make drugs and feed additive that has antibiotic, antivirus and growth improvement functions. The invention has the features of simple process method, and improving the growth benefit bacterium.

The invention discloses a Saccharomyces cerevisiae strain and a method for producing ethanol by fermenting stalks, the strain is Saccharomyces cerevisiae DQ1, the collection No. is CGMCC No.2528, the strain can tolerate methanoic acid, acetic acid and furfural type inhibitors in hydrolyzate of the stalks, tolerate high glucose concentration and ethanol concentration and have wide growth temperature, and the strain is fermented at high temperature; the method for producing the ethanol by fermenting the stalks comprises two-stage culture of seeds, wherein the first stage culture is carried out in a synthetic culture medium for 16-24 hours, the second stage culture is that a mixed culture medium containing 30-70 percent of hydrolyzate is inoculated into with the inoculation amount of 5-10 percent for culture for 15-20 hours, and the inoculation into the hydrolyzate with the inoculation amount of 10 percent or simultaneous saccharification and fermentation of the stalks is further carried out for producing the ethanol. The invention has the advantages of being applicable to high-solid-content stalk enzyme hydrolysis and simultaneous saccharification and fermentation, with short fermentation period, high ethanol yield, and low production cost, thereby having great industrial application prospect.

Provided is a recycle mixing type rice field culture method. According to the method, a rice field and fresh water resources are utilized for culturing. The method comprises the following steps of arrangement of the rice field, installation and adjustment of facilities, disinfection of the clean field and water fertilizing, rice planting, loach and Chinese soft shell turtle stocking, edible frog throwing, loach and edible fog harvesting, rice harvesting, water entering to flood the field, freshwater shrimp autumn seedling stocking, freshwater shrimp multiple fishing and coming into the market of freshwater shrimps, freshwater shrimp and Chinese soft shell turtle capturing, and ploughing and field drying, wherein the arrangement of the rice field comprises the excavation of culturing ditches, and trimming of field ridges. By the adoption of the method, rice-fish culture and freshwater shrimp autumn seedling and Chinese soft shell turtle mixed culture are organically and cyclically combined, the problems that an empty rice field in winter is not utilized, a water-phase food chain is simple, the mutually beneficial level of rice and fish is low and the bird prevention and the pest removal are low are solved thoroughly, the recycle and safe and efficient output of the rice field are ensured, the significant increasing of the benefits of rice field culture is achieved, and the method has the advantages that the culture process is easy to operate, the culture cost is low and the culture safety is high.

This invention relates to a preparation method for microbial colony used for effectively treating urban living sewage and sewage sludge in river bend, lake, marsh enclosed or semi-enclosed water areas. The said method includes the following steps: collecting and culturing of engineering initial bacterial strain, enrich mixed culture of bacterial strain, acclimatized culture of engineering bacteria and expanding culture of acelimatized bacteria. It this invention found is from the microbial growth and metabolic mechanism a preparation method of microbial colony which can high-effectively and high-adaptively treat various sewage sludges.

The invention discloses a method for mixed culture of crabs, shrimps and mandarin fishes. The method comprises the following steps: (1) water in a pool is 0.3-1.5m deep, the slope ratio of the pool is 1:3, high-protection facilities are built at the periphery of the pool, and an automatic aerator is arranged per 667m<2>; (2) the plant area of waterweeds in the pool accounts for one third of the area of the pool, and at the same time, snails are stocked; (3) at the end of March, 500-700 crabs per 667m<2> are stocked based on the standard of 80 crabs per kg, 5-7kg of post larvae of lobsters per 667m<2> are stocked in the first and middle third of April based on the standard, 7-10 mandarin fishes per 667m<2> (in 5 cm length) are put in the last third of May, and 6 kg of fingerling silver carps per 667m<2> are put in the first third of June based on the standard of 800-1000 fingerling silver carps per 1kg; (4) overhead price river crab feed cp.40% is used, supplementary with wheat and bean pulp, feed amount daily is controlled to 4-6% of the weight of shrimp and crab; and (5) a water quality regulator is used for one time per 15-30 days. On each day, shrimp and crab shell auxin and allicin, the amount of which account for 1-1.5% of the amount of feeds, are added.

The invention discloses a method for preparing a salt-tolerant microbial agent. The method comprises the following steps of in an open culture device, directly carrying out mixed culture on a salt-tolerant microbial flora in a high-salt environment sample under the condition that total salt concentration is 1%-30%; and adding an organic carbon source, nutrient elements and trace elements according to the needs in a culture process, controlling and keeping proper pH, temperature and dissolved oxygen relatively stable. The salt-tolerant microbial agent prepared by adopting the method has good salt tolerance and salinity impact resistance, can degrade organic substances in water without being restrained by high-concentration inorganic salt under the condition that total salt concentration is 1%-30%, and can be directly used for purifying high-salt-content wastewater with salt concentration of 1%-30%. The method disclosed by the invention is simple to operate, short in implementation period and capable of quickly carrying out industrial application.

This invention relates to a environmental friendly multifunctional soil conditioner, and it prepared by mixed culture fermentation with composting product heaping and retting by vegetal organic offal, such as, dry branches and fallen leaves, traditional medicine gruffs and coconut chaff, natural mineral material (maifanshi, bentonite, zeolite), ammonium 2, 4-dinitrophenolate, organic amendment (Acid precipitation of lignin, glutamate dregs and furfurol dregs) and micro-organism strain. This soil conditioner will outstandingly improve physical and chemical characteristic of soil and promote the formation of the soil coacervate. It can also neutralize acid base nature of the soil and improve the cation exchange capacity of the soil. It can also outstandingly improve the growth and reproductive of the crop and the availability of the fertilizer. It has the merits of using reproducible material, changing waste into resources, obviously ecological advantage and economic returns.

The present invention discloses a method for producing a fermented dairy product, preferably cheese, comprising inoculating milk with lactic acid bacteria capable of producing a Class IIa type bacteriocin; and lactic acid bacteria capable of producing a Class I type bacteriocin.

The invention discloses a method for producing oil by mixed culture of yeast and alga. In the method, industrial waste water is used as a culture medium for the mixed culture of yeast and alga, so as to produce microbial oil. The method is characterized in that compared with the single culture of two microorganisms, by the mixed culture of yeast and alga, the chemical oxygen demand (COD) of the waste water can be more effectively degraded and the yeast and alga are also promoted to reliably utilize the nutrient components in the industrial waste water, thereby being beneficial to the synthesis of the oil. The method for producing the microbial oil has the advantages of low cost and high practicable value, is simple to operate, namely, the pollution of high-concentration industrial waste water on environment can be reduced, and waste materials are changed into valuable things so as to produce shortage product oil, thereby achieving the purpose of recycling the waste materials.

A process is provided for increasing the production of PHA in a mixed culture biomass. In a first stage of the process, organic material associated with a substrate is converted to volatile fatty acids. In the case of a wastewater treatment process, if the wastewater includes sufficient volatile fatty acids (VFAs) to support the process, then it is unnecessary to convert organic material to VFAs. In a second stage of the process, an anaerobic-aerobic selection process is utilized to select glycogen accumulating organisms that cause these organisms to proliferate and dominate the open mixed culture biomass. By providing relatively high organic loading in the form of VFAs in the anaerobic treatment phase of the selection process, glycogen accumulating organisms having a relatively high level of stored glycogen are produced. In a third stage, the PHA accumulation process is practiced where the glycogen rich organisms are fed VFAs under anaerobic or aerobic conditions or combinations thereof. Through the consumption of externally supplied VFAs and internally stored glycogen, relatively high levels of PHA in the biomass are produced. Thereafter PHA is separated from the residual biomass.

The invention discloses a method for preparing a brown rice enzyme yogurt. The preparation method mainly comprises the steps of pretreatment of raw materials, homogenization, sterilization, cooling, addition of brown rice enzyme and a ferment, fermentation, cooling, filling and refrigeration. The addition of the brown rice enzyme and the ferment is as follows: 3-7 parts by weight of the brown rice enzyme and 4-6 parts by weight of the ferment are added in 100 parts by weight of mixed solution after the treatment for carrying out inoculation; and the ferment is mixed culture solution of streptococcus thermophilus and lactobacillus bulgaricus. The brown rice enzyme is formed by fermenting brown rice, dozens of types of new enzyme can be derived when having the nutrition of the brown rice, and the brown rice enzyme yogurt is further rich in gamma-aminobutyric acid, glutathione, gamma-oryzanol, ceramide, lipopolysaccharides from rice bran, minerals and other nutritional components. The brown rice enzyme yogurt mixes the dual functions of the brown rice enzyme and the yogurt, is easy to be absorbed by human body and can improve the immunity of the human body and activate cells, thereby being the beneficial complement to modern dietary nutrition.

The invention relates to a production method of an agricultural photosynthetic bacteria preparation, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35 DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heat disinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the agricultural photosynthetic bacteria preparation under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r / m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the preparation has the advantages of stable product quality and diversified functions.

The invention discloses anti-deterioration cellar mud and a preparation method thereof. Based on 100 percent of yellow clay, the anti-deterioration cellar mud comprises the following components: 3 to 5 percent of yeast powder, 8 to 10 percent of double bottom dreg, 5 to 8 percent of mixed culture solution of caproic acid bacteria and liquid cellar mud, 2 to 3 percent of M type microbial fermentation liquor, 15 to 20 percent of peat, 5 to 8 percent of cellar mud, 10 to 12 percent of yellow water, 1 to 2 percent of propionibacterium liquid, 4 to 6 percent of impure alcohol, 2 to 3 percent soybean cake powder and 0.05 to 0.1 percent of K2HPO4. The cellar mud realizes the rational regulation of pH value, moisture, humus, nutrient substance, N, P, K and the like by the regulation of a formula and the ratio of the mixed culture solution of the M type microbe to the propionibacterium liquid to the caproic acid bacteria to the liquid cellar mud is well regulated, so that the pH value of the active and excellent cellar mud is always maintained between 5 and 7.

The invention relates to a method for preparing a germinated brown rice ferment drink. Germinated brown rice is processed in an Aspergillus oryzae and Rhizopus oryzae mixed culture manner, is decomposed by using the enzyme system complementarity, and is inoculated with lactic acid bacteria to carry out fermentation. The prepared germinated brown rice ferment drink is rich in organic acids, variousfree amino acids, soluble dietary fibers, and active components which are gamma-aminobutyric acid and SOD, and has the advantages of sour-sweet mouthfeel, convenience in drinking, no additives, excellent nutrition and healthcare effects, great improvement of the raw material utilization rate in a two-stage fermentation process, and great solving of resource wastes of existing brown rice having bad mouthfeel.

The invention discloses a construction and stock breeding method for cultured grass carp families. The method comprises the following steps of: selecting grass carps which move actively and have high heritability and of which the weight is 15 to 20 percent more than the average weight, as parent fishes; marking cultured grass carps by using periodic interrupt timer (PIT) electronic labels, after sexual gland is matured, facilitating maturation by using hormone solution, performing artificial insemination on female and male parent fishes to obtain oosperm, performing self-hatching, then growing seedlings, breeding fishseed, breeding one-year fishes, feeding respectively, and establishing the families; from the second year to maturation time, injecting the PIT electronic label into the grass carps with the different families; performing mixed culture in a same pond, and eliminating the inferior grass carps, wherein the rest families are the basic families for next generation breeding; the young grass carps, the one-year grass carps, two-year grass carps, three-year grass carps and four-year grass carps are a critical period of family breeding; preferably choosing, and establishing the families serving as backup parent fishes; and then matching teams, raising the seedlings and then breeding continuously. A new product with high quality, high yield and stable yield is bred by establishing the different families of the cultured grass carps; and the method is used for researching the genetic characteristic and breed differentiation conditions by mating the male and female fishes among the grass carp families and inbreeding the male and female fishes in the grass carp family.

A process for preparing astaxanthin includes such steps as preparing special culture medium, loading it in reactor, inoculating algae including Haematococcacea able to generate astaxanthin and yeast in it, culturing and fermenting. Its advantages are low cost, and high conversion rate of saccharide, biomass and output of astaxanthin.

The invention provides a black tea fungus drink which is produced by the mixed fermentation of pure fungi. Three kinds of fungi of saccharomyces cerivisiae, bacillus aceticus and lactobacillus plantarum are compounded, and mixed and fermented to produce the black tea fungus drink. In the drink, the combination of the pure fungi and the mixed fermentation are utilized, and the formula of the culture medium of the black tea fungus and the fermentation process conditions are optimized, thereby obviously shortening the fermentation time, avoiding the pollution of mixed fungi and ensuring the product safety; management methods for culture, rejuvenation and preservation of the fungus are scientific and reasonable, the product quality is stable, and a good basis is laid for the industrialized production of the black tea fungus; and the prepared black tea fungus drink not only tastes good, but also contains various effective components, and is a microbial fermentation drink with high utility value.

The invention relates to efficient desulfurizing bacreria and use method thereof in removal of sulfur in iron ore, the desulfurizing bacreria is a mixed culture of acidithiobacillus ferrooxidans, leptospirillum ferriphilum and ferroplasma acidiphilum, the desulfurizing bacreria is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.8337. The method includes the following steps: (1) the sulfurous iron ore is broken and ground into ore powder; (2) the ore powder is prepared into ore pulp by use of water; (3) the ore pulp pH is adjusted to 1.0-3.5, the mixed bacteria liquid of the desulfurizing bacreria is inoculated; and (4) oxidation process temperature is controlled at 10 DEG C to 55 DEG C, and the period is 5-60 days. The efficient desulfurizing bacreria is suitable for the microbial oxidation desulfurization of various kinds of sulfurous iron ores, the investment is little, the production cost is low, and no waste gas and environment pollution are produced.

The invention relates to the biological fermentation technology, in particular to a compound microbial starter, and a method which uses the compound microbial starter to produce high-energy protein biological feed. The compound microbial starter selects cellulomonas sp dinitrogen, cellulomonas flavigena, condensate bacillus, lactobacillus acidophilus, bacillus subtilis, lactobacillus starch, compound enzyme bacteria, propionic acid bacteria, candida yeast, saccharomyces diastaticus, saccharomyces cerevisiae, as well as aroma-producing yeast as the bacteria sources, and makes use of the modern biotechnology to prepare various microbial agents; the microbial agents are then treated with anaerobic fermentation with the discarded sweet corn cobs, corn stalks, pea skins, abandoned vegetables and other discarded crops, so as to obtain the high-energy protein biological feed required in livestock and poultry breeding industry. The invention solves the problem of low utilization rate of crop resources; and the high-energy protein biological feed can improve the palatability of feed, increase the enzyme content of the feed, provide an effective way for developing the animal husbandry and solving the food conflicts between people and livestock, as well as reduce the pollution to the environment caused by the traditional crop waste burning.

A microalgae suspension-adhesion mixed culture and separated harvesting method based on a suspended carrier comprises the following steps: on the basis of original microalgae suspension culture, a predetermined amount of solid carrier is added into a culture solution and enabled to suspend in the liquid phase in an aerating or mechanical stirring manner; during cultivation, the microalgae is enabled to attach to a solid carrier to form an algae membrane; the solid carrier is separated to harvest algae cells; the solid carrier from which the algae cells are harvested is recovered for recycling and re-injected into the microalgae culture solution; serving as seeds, the liquid-phase algae cells are re-attached to the solid carrier to grow, so that continuous culture and harvest of the algae cells are realized. Compared with the air floatation, precipitation, centrifugation, filtering and other harvest methods, the microalgae suspension-adhesion mixed culture and separated harvesting method is simple to operate, dispenses with agents, is low in energy consumption, and can realize large-scale and mechanical continuous harvesting, thereby effectively saving the cost in harvesting the algae cells.

The invention provides a method for producing microecological feed additives for livestocks by using agricultural and sideline products. The method comprises the following steps of: mixing agricultural and sideline products main ingredients, agricultural and sideline products auxiliary materials and starch in parts by weight so as to obtain culture medium; mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a viable count proportion of 1-3:0.5-2:0.5-3 so as to prepare mixed culture; mixing the culture medium and distilled water, and then inoculating; evenly stirring and fermenting; and after fermenting, naturally drying so as to obtain the final product. The microecological feed additives for the livestocks prepared by the agricultural and sideline products contain rich beneficial bacteria such as yeast, lactic acid bacteria and bacillus subtilis, metabolites of the beneficial bacteria, rich amino acid, low molecular peptide and growth promotion active ingredients; after the livestocks ingest, the growth and development of the beneficial microorganisms in alimentary canals of the livestocks can be promoted, the digestion function can be improved, certain nutrient substances can be provided for the livestocks, the production performance of the livestocks can be improved, and the health situation of the livestocks can be improved.

The invention relates to a culture medium composition suitable for culturing ordinary chlorella and a method for mixed culture of the chlorella by using the culture medium. The culture medium is obtained by adding 0.00126-0.063 g / L of Na2SO3 and an organic carbon source based on a common SE culture medium. The use of the culture medium in photoautotrophy-heterotrophy mixed culture of the ordinarychlorella can remarkably promote the growth of chlorella, and can allow the cell density of the ordinary chlorella to reach a level of high density culture in a short culture period, that is, the high density culture of the chlorella is realized. The use of the culture medium composition allows the ordinary chlorella to easily use the organic carbon source, especially a cheap carbon source for growing, can promote the later grease accumulation, and can greatly increase the quality of the ordinary chlorella, i.e. the grease content of the ordinary chlorella compared with heterotrophic culture to reach the level of photoautotrophic culture.

The invention provides a process for producing fermented soybean meal through the solid state fermentation of mixed culture. A homemade breeding culture medium can be used for simultaneously breeding and culturing four kinds of cultures and then obtained culture solutions are used for fermenting the soybean meal; few breeding and fermentation procedures are adopted, the process is easy in operation and easy to achieve large-scale production; the fermented soybean meal is low in water content, loose, non-viscous and short in drying time, so that the production cost is lowered; in addition, the drying is performed at a relatively-low temperature, so that the biological activity of probiotics in the soybean meal is maintained. The process disclosed by the invention is used for fermentation production of the soybean meal by using mixed cultures of candida utilis, bacillus subtilis, lactobacillus and bifidobacterium; the fermented soybean meal contains a great number of probiotic groups, and a trypsin inhibitor is removed effectively; as an animal feed, the fermented soybean meal can be used for improving the immunity of animals and reducing the occurrence of diseases. Used as the animal feed, the fermented soybean meal produced by adopting the process disclosed by the invention is relatively easy to digest and absorb and can be used for replacing fish meal to be applied to aquaculture feeds and pig and chicken feeds.

The invention relates to a microbiological additive for ruminants and a preparation technology thereof. The additive is characterized by culturing respectively Saccharomyces cerevisiae, Candida utilis and Bacillus subtilis in a liquid culture medium to obtain Saccharomyces cerevisiae fermentation bacterial liquid, Candida utilis fermentation bacterial liquid and Bacillus subtilis fermentation bacterial liquid, mixing the three fermentation bacterial liquids, and then carrying out a fermentation process of the three fermentation bacterial liquids on a solid culture medium to obtain a mixed culture, wherein in the mixed culture, a polypeptide content is equal or greater than 5.3 g / Kg; a beta-glucan content is equal or greater than 125 mg / Kg; by weight, an amino acid content is equal or greater than 16.5%; and by weight, an organic acidity content is equal or greater than 3.45%. The invention also relates to a preparation method of the microbiological additive for ruminants.

The invention discloses an entrapping immobilization method of mixed anaerobic ammonium oxidation culture, which comprises the steps of obtaining embedding medium, mixing the embedding medium and sludge, obtaining immobilized anaerobic ammonium oxidation beads, activating the immobilized anaerobic ammonium oxidation beads, and the like. The obtaining of the embedding medium is to weigh polyvinyl alcohol and sodium alginate to be made into solution, according to the mass percent, the concentration of the polyvinyl alcohol is 6%-10wt% and the concentration of the sodium alginate is 1%-2wt%, and the polyvinyl alcohol and the sodium alginate are well stirred to make into mixed embedding medium. The method is simple in technique, simple in operation and low in costs. The entrapping of immobilized ANAMMOX mixed culture can be successful, the method can design an anaerobic ammonia oxidation reactor which is highly effective and specific in action, and the anaerobic ammonia oxidation reactor and a lead nitrosation reactor can be combined to form a high-efficiency stable biological denitrification system, which is adaptable for commercial manufacture and can bring about perfect economic and environmental benefits.

The invention relates to the formula of a special culture material capable of increasing the content of each amino acid in a grey mushroom fruiting body and a culture method. The formula provided by the invention is characterized in that the formula of the special culture material consists of the following raw materials in part by weight: 20 to 30 parts of straw, 20 to 30 parts of sawdust, 20 to 27 parts of cottonseed hull, 10 to 15 parts of bran, 0.3 to 0.5 part of monopotassium phosphate, 0.2 to 0.3 part of magnesium sulfate and 1.0 to 1.2 parts of calcium carbonate or calcium sulfate, wherein 10 to 30 kilos of pig excrement residues are added into 100 kilos of mixed culture material. According to the invention, the grey mushroom fruiting body has a round and attractive mushroom flower and greatly increases the content of each amino acid in the grey mushroom fruiting body, wherein the content of sulfur amino acid, branched chain amino acid, children amino acid, essential amino acid, flavor amino acid and sweet amino acid is greatly increased respectively. The grey mushroom fruiting body can increase the health-care medicinal function, increase the production additional value, reduce pollutants in the pig breeding industry and recycle the pig excrement residues.