Traditional Chinese medicine solid type mixed culture fermentation tech. and its application

A solid fermentation and multi-strain technology, applied in the fields of application, bacteria, fungi, etc., can solve the problems that the technology has not been reported and the influence of multi-strain is not very clear, and achieve the effect of improving drug efficacy and enhancing effect

Inactive Publication Date: 2006-09-13
NANJING SBEED BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to differences in the growth conditions of different strains, the mutual influence of multi-strain mixed growth is not very clear. The current research is mostly focused on the research field of a single strain on specific Chinese medicinal materials, screening the compatibility of different strains and There is no report on the technology of fermenting single or multiple Chinese medicinal materials, and there is no report on the use of Chinese herbal medicine multi-strain solid fermentation technology for livestock and poultry breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Chinese medicine antiviral formula:

[0034] Qingwenbaidu Powder: 120g gypsum, 30g rehmannia, 60g buffalo horn, 20g coptis, 30g gardenia, 20g bark of Moutan, 25g scutellaria baicalensis, 25g red peony, 25g scrophulariaceae, 30g Anemarrhena, 30g forsythia, 25g bellflower, licorice 15g, light bamboo leaves 25g.

[0035] Fermentation process:

[0036] Bacillus cereus DM423 and Bacillus subtilis CICC10071 were respectively inoculated into common broth culture medium at 37°C and 250rpm shaker to produce first-grade seed liquid. When the bacterial content reached 1.0×10 7～8 When CFU / ml, the primary seed liquid is transferred to the secondary seed liquid fermenter for expanded cultivation (common broth), the inoculum size is 10% of the total mass of the fermented product, the culture temperature is 25-30°C, and the stirring speed is 250rpm. Bacterial content reaches 1.0×10 7～8 CFU / ml, then inoculated into the production tank for fermentation at 25-30°C (taking the total mas...

Embodiment 2

[0039] Traditional Chinese Medicine Antibacterial Prescription:

[0040] Four Flavors Chuanxinlian San: 450g Andrographis paniculata, 150g Polygonum japonica, 200g Daqingye, 200g gourd tea.

[0041] Fermentation process:

[0042] Inoculate Bacillus licheniformis CICC10098 into ordinary broth, Lactobacillus CGMCC1.1878 and Lactobacillus acidophilus CGMCC1.9 were inoculated into MRS medium respectively, and cultured on a shaker at 37°C at 250rpm to produce a first-class seed liquid. When the bacterial content reached 1.0×10 7～8 When the CFU / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculation amount is 10% of the total mass of the fermented product, the culture temperature is 28±2°C, the stirring speed is 250rpm, and the bacterial content of the secondary seed liquid reaches 1.0×10 7～8 CFU / ml is inoculated into production tanks for fermentation at 25-30°C (taking the total mass of the fermented product as a reference, the inoculum ...

Embodiment 3

[0045] Chinese medicine growth-promoting formula:

[0046] Fat pig powder: Mianma Guanzhong 30g, Polygonum multiflorum 30g, Malt 500g, Soybean (fried) 500g.

[0047] Fermentation process:

[0048] Inoculate Bacillus subtilis CICC10071 and Bacillus licheniformis CICC10098 into ordinary broth respectively, Lactobacillus Lactobacillus CGMCC1.1878 and Lactobacillus acidophilus CGMCC1.9 were inoculated into MRS medium respectively, and cultured on a shaker at 37°C and 250rpm to produce first-class seeds solution, when the bacterial content reaches 1.0×10 7～8 CFU / ml is then transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculation amount is 8% of the total mass of the fermented product, the culture temperature is 28±2°C, the stirring speed is 200rpm, when the bacterial content of the secondary seed liquid reaches 1.0×10 7～8 CFU / ml is inoculated into the production tank and fermented at 25-30°C (taking the total mass of the fermented product as a ...

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Abstract

The invention discloses a Chinese drugs multi-bacterium species mixture solid fermenting method and its application. It is made up from crushing the Chinese drugs, filtering, adjusting water content and pH value, adding bean pulp, bran, and inorganic salt, sterilization, cooling and adding bacterium species fermenting liquid to take ferment, and drying. The invention could be used to make drugs and feed additive that has antibiotic, antivirus and growth improvement functions. The invention has the features of simple process method, and improving the growth benefit bacterium.

Description

technical field [0001] The invention belongs to the field of biological fermentation and relates to a traditional Chinese medicine multi-bacteria mixed solid fermentation technology, and also relates to the application of the technology in the preparation of medicine or green feed additive in the livestock and poultry breeding industry. Background technique [0002] Today, as the pace of economic globalization is accelerating and the degree of market opening is expanding, my country's animal husbandry industry has gained new opportunities for accelerated development, but at the same time it is also facing more intense market competition. On the one hand, in the international market, developed countries have higher and higher requirements on the quality, sanitation, and safety of our livestock and poultry products, and the threshold of green trade barriers has been continuously raised, which has become a restriction on the export of my country's livestock and poultry products....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A23K1/16A23K10/12A23K10/18
Inventor 储卫华
Owner NANJING SBEED BIOTECH
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