The invention belongs to the technical field of
biodegradation treatment of contaminated soil and water, and discloses an enrichment, acclimation and
screening method for an
anaerobic degradation community of
polycyclic aromatic hydrocarbon and application thereof. A mixture of an
electron acceptor,
sodium sulfide and the
polycyclic aromatic hydrocarbon is added in the contaminated soil according to the
mass ratio and is sealed and stood for 6 months, and then a mixed liquid of a basal culture medium, a micro
metal liquid and a
vitamin C solution is added into an anaerobic box full of
nitrogen according to the proportion for soil
acclimatization; the
polycyclic aromatic hydrocarbon, the basal culture medium, the micro
metal liquid, the
vitamin C solution and an adsorbent are added into an anaerobic
bottle in the anaerobic box full of
nitrogen, the enriched and acclimatized soil is grafted into the anaerobic
bottle according to the
mass ratio of water to the soil, and the
electron acceptor and the
sodium sulfide are added according to the
mass ratio. The soil is cultured for 20 to 25 days in a rotary oscillator with a temperature of between 18 and 22 DEG C and then is subjected to circular
inoculation for more than 5 times to obtain a microbial
community used for anaerobically degrading the polycyclic
aromatic hydrocarbon. The method can enrich, acclimatize and screen the microbial
community with good
anaerobic degradation performance, and the degradation rate range of the polycyclic
aromatic hydrocarbon is between 0.01 and 1.04mg.L<-1>.d<-1>.