1738results about How to "Increase biomass" patented technology

The invention relates to an immobilized microorganism preparation method in biochemical technical field, comprising the following steps: step 1, preparing a solution of which preparation method per 100ml comprises the following steps: (1) dissolving 12-18g of polyethylene glycol prepolymer in water to obtain a solution A; (2) adding microorganism in the solution A, then adding 0.5-1g of N,N-dimethylbisacrylamide and 0.05-0.1g of silicon dioxide, then adding 0.05-0.2g of diethoxyacetophenone and obtaining a solution B; (3) adding water in the solution B to ensure the volume of the obtained solution is 100ml, mixing evenly and obtaining the desired solution; step 2, placing the solution obtained in the step 1 under a ultraviolet lamp for curing, cutting, and washing to obtain the immobilized microorganism. The method of the invention can be performed at room temperature, the reaction process is easy to control and the prepared embedded bacteria particles have longer service life.

A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow.The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.

In a wastewater treatment system and process utilizing membrane bioreactors (MBRs), multiple, parallel series of tanks or stages each include, an MBR stage. Under conditions of normal flow volume into the system, influent passes through several parallel series of stages or process lines, which might be, for example, an anoxic stage, an aeration stage and an MBR stage. From the MBR stages a portion of M.L.S.S. is cycled through one or more thickening MBRs of similar process lines, for further thickening and further processing and digesting of the sludge, while a majority portion of the M.L.S.S. is recycled back into the main process lines. During peak flow conditions, such as storm conditions in a combined storm water / wastewater system, all of the series of stages with their thickening MBRs are operated in parallel to accept the peak flow, which is more than twice normal flow. M.L.S.S. is recycled from all MBR stages to the upstream end of each of all the parallel process lines, mixing with influent wastewater, and the last one or several process lines no longer act to digest the sludge. Another advantage is that with the thickened sludge in the last process line of basins, which ordinally act to digest the sludge, there is always sufficient biomass in the system to handle peak flow, the biomass being available if needed for a sudden heavy flow or an event that might bring a toxic condition into the main basins.

Polynucleotide sequences and methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and / or increasing the biomass and / or increasing the yield of a plant are provided.

The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including increased biomass or improved cold or other osmotic stress tolerance, as compared to wild-type or reference plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.

Regulation of expression of programmed cell death, including senescence, in plants is achieved by integration of a gene or gene fragment encoding senescence-induced deoxyhypusine synthase, senescence-induced eIF-5A or both into the plant genome in antisense orientation. Plant genes encoding senescence-induced deoxyhypusine synthase and senescence-induced eIF-5A are identified and the nucleotide sequences of each, alone and in combination are used to modify senescence in transgenic plants.

The invention concerns the use of recombinant clonotypic immunoglobulins (Ig) as a vaccine in the treatment of HCV-related and non HCV-related lymphoproliferations, in particular the use of recombinant proteins with immunogenic properties derived from protein segments VK3-20 and VK3-15 of Ig light chains derived from patients with lymphoproliferations.

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with the standard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

The invention relates to an organic-inorganic composite fertilizer, which consists of straws, excrements and fertilizers. By weight, in the composite fertilizer, the ratio of N, P2O5 and K2O is 10 to 35:8 to 12:5 to 8, and the ratio of C and N is 10:15. The organic-inorganic composite fertilizer has very obvious effects for improving biomass of wheat and increasing readily available nutrient of soil, urease activity, microbial biomass in the carbon-nitrogen aspect.

The invention discloses ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp, wherein the ankistrodesmus strain is SS-B7, the classification name of SS-B7 is Ankistrodesmus sp, and the ankistrodesmus sp SS-B7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7478. The ankistrodesmus sp SS-B7 has tolerance to CO2 and NOx with high concentrations, and has high carbon sequestration efficiency, the content of total lipids in the cells of the obtained biomass accounts for 40% or above of the dry cell weight, and the ankistrodesmus sp SS-B7 can be used for producing biodiesel.

The invention relates to heavy metal resistance plant growth-promoting bacteria and a preparation applying method thereof, which belongs to the bioremediation field of heavy metal polluted environment. A bacteria strain D54 with a preservation number of CGMCC No. 3223 belongs to the Burkholderia sp. and has higher resistance to a plurality of heavy metals, wherein the resistances to Pb <2+>, Cd <2+>, Cu <2+>, Zn <2+> respectively reach 800 mg / L, 1500 mg / L, 150 mg / L and 2500 mg / L. In addition, the bacteria strain D54 has the plant growth-promoting functions of producing plant growth hormone (IAA), producing 1-amino-1-carboxyl cyclopropane (ACC) deaminase, secreting siderophore, dissolving inorganic phosphate, fixing nitrogen and the like, has the biological prevention functions of antagonizing plant pathogenic bacteria inbreak and the like, and can obviously improve the biomass of the plants applied the invention and improve the resistance to diseases and stresses. The number of the effective viable bacteria in liquid preparation reaches 1-2 billion / ml and the number of the effective viable bacteria in solid preparation reaches 1 billion / g. Soaking seeds for 1-2 hours in the liquidpreparation which is diluted 100 times and irrigating the diluted liquid preparation 1-2 times (10ml / kg) after 2-3 weeks of the sprouting of the seeds can effectively improve plant viable bacteria infection probability.

Polynucleotide sequences and methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and / or increasing the biomass, vigor and / or yield of a plant are provide

The invention discloses an ecological ditch for increasing utilization rates of N and P in a farmland by controlling agricultural non-point source pollution. According to the ecological ditch for increasing utilization rates of N and P in the farmland by controlling agricultural non-point source pollution, aiming at the characteristics of the agricultural non-point source pollution, by using an ecology principle and a biological characteristic, through increasing the biomass of a ditch wetland ecosystem and using a synergistic effect of a system, water body pollutants are blocked, degraded, absorbed and utilized so as to achieve the purposes of reducing the pollutants discharged from a water body and increasing the utilization rates of the N and the P in the farmland.

An oxygen emitter which is an electrolytic cell is disclosed. When the anode and cathode are separated by a critical distance, very small microbubbles and nanobubbles of oxygen are generated. The very small oxygen bubbles remain in suspension, forming a solution supersaturated in oxygen. A flow-through model for oxygenating flowing water is disclosed. The use of supersaturated water for enhancing the growth of plants is disclosed. Methods for applying supersaturated water to plants manually, by drip irrigation or in hydroponic culture are described. The treatment of waste water by raising the dissolved oxygen with the use of an oxygen emitter is disclosed.

Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NO: 26, 106, 107, 109, 110, 112, 114, 115, 118, 119, 122, 123, 124, 126, 95 or 96, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and / or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass / vigor / yield.

The invention belongs to the technical field of livestock breeding and specifically relates to an ecological method of breeding chicken by planting grass under trees. The method includes that a fine forage grass breed which is shade-tolerant, trampling-resistant, strong in stress resistance and applicable to being planted in the forest is selected, and a forest land for grazing the chicken is modified to form a grassland under the trees for grazing the chicken; a contour grass and shrub zone in a stocking land is used for planting the grass, a chicken house is built in the middle of the grassland under the trees for grazing the chicken, zone division is performed for rotation grazing, and zones are separated by biological fences; and Chinese herbal plants are sowed in rotation grazing zones so that the grazed chicken can freely ingest the plants. A scientific evidence is provided for obvious economic benefits and ecological benefits which are achieved by means of rapid development of domestic fowls and effective ecological rehabilitation, wherein the domestic fowls of Hetian chicken and the like are applicable to stocking in forestland. By means of the model for breeding the chicken, high economic benefits are created, parts of the economic benefits are used for investing development of ecological chicken breeding model, and the created economic benefits and the development of ecological chicken breeding model are combined for creating better benefits, and accordingly, a virtuous cycle is achieved, ecological balance is kept, and the sustainable development is effectively achieved.

Provided are methods of increasing the tolerance of a plant to abiotic stresses and / or increasing the biomass and / or increasing the yield of a plant by expressing within the plant an exogenous polynucleotide homologous to SEQ ID NO:13.

The invention discloses a method for treating coking wastewater, which belongs to the field of wastewater treatment. The method comprises the following steps that: wastewater of a coking plant enters a regulating reservoir for the adjustment of water quality and water amount, and orderly enters a hydrolytic tank, an anaerobic tank, a preaeration tank, a preliminary sedimentation tank, an aerobic tank, a secondary sedimentation tank and a coagulation reaction tank for treatment, wherein the hydrolytic tank and the preaeration tank are filled with a bio-cord filler; the sludge of the preliminary sedimentation tank refluxes into the hydrolytic tank and the anaerobic tank respectively; a clarifying solution of the secondary sedimentation tank refluxes into the anaerobic tank; and the aerobic tank adopts a moving bed bio-film reactor. The bio-cord filler adopted in the invention has short film formation time and high biomass, does not need repeated washing, and has strong shock resistance; and the moving bed bio-film reactor can fix a large amount of nitrobacteria with long generation time, and has no the problem of blocking. The method can remove high-concentration ammonia nitrogen and organic substances in the coking wastewater, and the COD and the ammonia nitrogen of treated effluent can synchronously achieve the primary standard of the national integrated wastewater discharge standard (GB9878-1996).

The invention discloses nano biological filler for purifying aquaculture wastewater in a biological filter tank and a preparation method of the nano biological filler. The biological filler comprises an EM rejuvenation solution and an adsorption carrier of the EM rejuvenation solution, wherein the EM rejuvenation solution is a product obtained by performing mixed fermentation on an EM stock solution, molasses and deionized water; the adsorption carrier is obtained by performing mixed pressing on composite nano powder, kaolin powder and modified bentonite; the composite nano powder is a product obtained by performing mixing and high-temperature sintering on nano-level aluminum oxide, titanium dioxide and silicon dioxide; modified bentonite is prepared from sodium diethyldithiocarbamate modified original soil. The preparation method comprises the following steps: pressing adsorption carrier particles; fermenting to obtain the EM rejuvenation solution; dipping the EM rejuvenation solution by using the adsorption carrier to obtain the nano biological filler. The obtained nano biological filler is high in pore volume, high in cell adsorption capacity, large in specific surface area and strong in stability, has a good treatment effect on aquaculture sewage, and can be used for rapidly removing ammonia nitrogen, phosphorus and heavy metal ions in an aquaculture water body.

The invention provides a composite repair agent for soil heavy metal pollution as well as a preparation method and application thereof, and belongs to the technical field of soil improvement and environmental protection. A composite repair agent for soil cadmium pollution is prepared from the following components in parts by weight: 10 to 30 parts of sodium bentonite, 5 to 15 parts of bone powder,5 to 25 parts of chitosan, 5 to 30 parts of oyster shell powder, 10 to 40 parts of graphene-modified crop straw biochar, 10 to 30 parts of bio-organic fertilizer, 15 to 45 parts of sodium silicate, 1to 15 parts of nano-FeO, 1 to 8 parts of carbon nanotubes, and 1 to 10 parts of microbial inoculants. The number of each 10 mg colonies of rhodotorula mucilaginosa OP11 in the microbial inoculum is 2.25*10 <8> to 2.5*10<9> CFU; the preservation number of the rhodotorula mucilaginosa OP11 is CGMCC No. 13540. The application of the composite repair agent is in remediation of heavy metal contaminated soil.

The invention discloses a method for preparing a bio-carrier by porous foam polymer modification. The method is used for coating bonding modification of porous foam polymers. The method comprises the following steps of adding one or more cationized porous high-adsorptivity functional materials and one or more cationized polyhydric hydrophilic functional materials into coating modification slurry, mixing the coating modification slurry and a cationic acrylic acid emulsion, carrying out coating bonding of the mixture on one or more porous foam polymers, and carrying out cross-linking curing to obtain the modified bio-carrier having strong adsorbability, hydrophily, surface active functional group diversity, positive surface charges and strong biocompatibility. The method provided by the invention is not limited by structures, shapes, aperture sizes and physical properties of foam polymers and can be widely used for external coating modification of multiple carrier materials. The method provided by the invention has the advantages of convenient and simple operation, low investment and making cost, excellent carrier performances and long service life. The bio-carrier obtained by the method can be widely used in fields of fixed attachment of various microorganisms, and enhanced biological treatment.

The invention discloses a method for ecological restoration and water quality conservation of river water. according to the method, an aeration oxygenation system consists of a solar cell panel, a support connector, an air pump, an aeration tube and an air distribution plate and is used for maintaining the dissolved oxygen level in the water; an ecological floating bed system consists of a strong buoyancy carrier and an emergent aquatic plant and is used for removing pollutants out of water and gathering and transferring heavy metal; and a filler system is used for providing a lot of adhesion habitats for microbial flora, phytoplankton, protozoon and tiny metazoan so as to co-build and form a biological membrane which efficiently and quickly purifies the pollutants. By adopting the method, the organic matters such as COD, nitrogen, phosphorus and the like, and other pollutants in the water can be efficiently removed, the water is purified, an ecological system of the river way is improved, and the self-cleaning capacity of the water is restored.

The invention discloses a pyridine-degrading bacterium as well as a composite bacterial agent, a preparation method and uses thereof. The newly-separated Rhodococcus pyridinovora KT-J002 CGMCC No.2789 and Cellulomonas sp KT-J007 CGMCC No.2788 not only can effectually degrade the high concentration pyridine, but also has tolerance or degradation ability for toxic substances such as benzene, phenol, dimethylbenzene, quinoline, cyanide and the like; the composite bacterial agent composed of Rhodococcus pyridinovora KT-J002 CGMCC No.2789, Cellulomonas sp KT-J007 CGMCC No.2788, Paracoccus d enitrificans W12 CGMCC No.1673, Micrococcus luteus ATCC 49442 and Arthrobacter crystallopoietes ATCC 15481 is used for environmental improvement, can effectually degrade the pyridine and is particularly suitable for the biological treatment of coking waste water.

The invention relates to a sewage double-biomembrane deep treatment technology comprising the steps: sewage enters an anaerobic tank, an anoxic tank and an oxygen maintaining tank after being pretreated by a grid and settled sand, and mud releases phosphorus in the anaerobic tank; the sewage is denitrified in a denitrification mode in the anoxic tank so as to remove most of organic matters; cilium biological fillers are arranged in the oxygen maintaining tank, biomembranes are attached to the cilium, absorb the phosphorus in excess and degrade the partial organic matters; the sewage enters a secondary sedimentation tank to carry out sedimentation treatment through the oxygen maintaining tank and then enters an aeration biological filter tank; granule biological fillers are arranged in the aeration biological filter tank, and the biomembranes are attached to granules, degrade the residual organic matters in the sewage and convert ammonia nitrogen into nitrate nitrogen; one part of the discharged water of the aeration biological filter tank flows back into the anoxic tank, and the other part is discharged; one part of the mud generated by the secondary sedimentation tank back flows into the anoxic tank, and the other part of the mud is discharged out of a system. The sewage double-biomembrane deep treatment technology has the advantages of investment saving, low operation cost, little land occupation and good treatment effect.

Isolated polynucleotides are provided. Each of the isolated polynucleotides comprise a nucleic acid sequence encoding a polypeptide having an amino acid sequence at least 80% homologous to SEQ ID NOs:130-258 and 536-791, wherein the polypeptide is capable of regulating cotton fiber development. Also provided are methods of using such polynucleotides for improving fiber quality and / or yield of a fiber producing plant, as well as methods of using such polynucleotides for producing plants having increased biomass / vigor / yield.

The invention relates to a method for intercropping sorgho and sesbania on a saline and alkaline land, and the method comprises the following steps of: A, soil desalting and land preparation: desalting the plowed layer soil of the saline and alkaline land, leveling the land, fertilizing, and seeding; B, intercropping, wherein the intercropping row number ratio of the sorgho and the sesbania is 1:3, namely one row of the sorgho is intercropped with three rows of the sesbania, the row spacing of the sesbania and the sorgho is 25-30 cm, the row spacing of the sesbania is 33-35 cm, and the row spacing of the sorgho is 100-105 cm; C, field management: thinning or filling seedlings when the sorgho has 2-3 leaves and the sesbania has 3-4 leaves, and singling one strain in every hole; and D, harvesting: harvesting the sorgho 7-10 days before grains are mature, reaping stalks and seeds, and reaping the sesbania before the grains are mature and cracked. The method disclosed by the invention promotes the balanced yield increase of the sorgho and the sesbania by utilizing the advantage complementary action of a symbiotic system of the sorgho and the sesbania, reduces the fertilizer addition, effectively remits the nutrient pinch obstruction of the saline and alkaline land, alleviates the secondary salinization of soil and enhances the composite utilization ratio of the land.