741 results about "Squalene" patented technology

The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably CARBOPOL®, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polyoxypropylene block copolymer such as PLURONIC®. The vaccine composition may optionally include a preservative, preferably thimerosol and / or EDTA. In another embodiment, the invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration, and comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant or adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity, in combination with other vaccine components.

The invention belongs to the field of microbial fermentation engineering, and discloses schizochytrium limacinum (Aurantiochytrium sp.SD116). The schizochytrium limacinum is stored in China Microbial Culture Collection Administration Committee General Microbial Center with a number of CGMCC No: 6208), and a method for fermenting and producing DHA (Docosahexaenoic Acid) grease utilizing high density of the schizochytrium limacinum. According to the schizochytrium limacinum and the method disclosed by the invention, a bacterial strain fermenting condition is optimized based on element supply and a fermenting control angle, a carbohydrate supplementation operation is carried out, therefore, high-density fermentation is achieved, dry cell weight finally reaches 70.43g / L, grease content reaches 50.1g / L, DHA occupies more than 35% of total content of fatty acid; moreover, bioactive substances such as beta-carotene, astaxanthin and squalene are contained. The complete set of technology is convenient to operate; higher biomass liveweight and DHA content can be obtained; fermenting cost can be reduced; and the method is suitable for industrial fermenting production.

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with the standard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The invention relates to a method for extracting squalene from vegetable oil deodorized distillate. A process method of combining molecular distillation with extraction and crystallization is used to extract high-concentration squalene and recover natural vitamin E and phytosterol with a certain purity from vegetable oil deodorized distillate. The method comprises the following steps of: carryingout a saponification reaction of the raw material; twice molecularly distilling unsaponifiable matter obtained by extraction; using the distillate obtained during the second distilling as a raw material for cold crystallization to obtain phytosterol; recovering squalene and mixed tocopherol from the filtrate; and extracting with multi-stage solvents to respectively enrich squalene and vitamin E in two phases. The process has simple flows, low-prices raw material and higher separation efficiency and recovery rate and comprehensively utilizes useful contents in the deodorized distillate.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The invention provides a method for preparing squalene based on tobacco as a raw material. The method comprises the following steps: smashing dried tobacco leaves, leaching out fat-soluble substances with an organic solvent, and removing the organic solvent in the fat-soluble substances so as to obtain crude tobacco squalene; passing the crude tobacco squalene through a silica gel chromatograohiccolumn, eluting with the organic solvent, collecting eluent, and removing the organic solvent by using a decomposition distillation method so as to obtain the fine squalene. The content of the squalene in the crude squalene is more than 35%, the content of the squalene in the fine squalene is more than 95%. Tobacco resources are abundant and can meet industrial requirement; in the method, waste tobacco and cigarette fine waste in the cigarette production can be fully utilized to prepare the squalene; and according to the invention, a technical basis is provided for development and utilizationof the tobacco squalene, especially for the application of the tobacco squalene in the aspect of cigarette production. Thus, the social benefits and economic benefits are great.

The invention discloses an oil-in-water emulsion free of surfactant. The emulsion comprises a metabolizable oil phase, a water phase and oil-water amphipathic solid particles dispersed in the water phase and having biocompatibility, wherein the oil phase comprises squalene or / and tocol; the water phase is any one or a combination of at least two of purified water, water for injection, aqueous liquid of glycerol, buffered saline liquid and clinically available infusion liquid; and the average grain diameter of the solid particles is at nanometer-to-micron grade. The emulsion can be used as a vaccine adjuvant or a medicine delivery or controlled release carrier; the properties of the emulsion can be controlled and regulated; the obtained emulsion is stable; the use of a surfactant is avoided; and harm to the human body and pollution on the environmental can be reduced.

The invention relates to a method for extracting high-purity squalene by taking olive oil as a raw material. A technological route formed by adopting a secondary molecular distillation and silica gel column chromatography is as follows: an olive oil unsaponifiable substance is taken, squalene is separated and purified by using two stages of molecular distillation, primary molecular distillation is carried out under the conditions of the evaporation surface temperature of 100 to 200 DEG C, the systemic pressure of 0.001 to 0.01mbar and the scraping film rotor speed of 150 to 300rpm, and secondary molecular distillation is carried out to the obtained distillate; the secondary molecular distillation is carried out under the conditions of the evaporation surface temperature of 150 to 300 DEG C, the systemic pressure of 0.001 to 0.01mbar and the scraping film rotor speed of 200 to 350rpm, and the obtained distillate is a squalene crude product; ethyl acetate-normal hexane with different concentrations is used as a mobile phase to carry out gradient elution, the obtained eluent is collected according to time and a solvent is evaporated, the same fractions are merged through chromatographic detection, and the high-purity squalene can be obtained, wherein the content of the raw material olive oil of the squalene is enhanced from 3.6% to about 98%; and especially, by considering the requirement of industrialized production to select an extraction condition especially, the large-scale production of the squalene taking the olive oil as the raw material can be realized.

The invention describes an overall process for the extraction of sterols, vitamin E, squalene and other plant-based hydrocarbons from deodorization distillates of plant oils. After an esterification of the free fatty acids, then a transesterification of the combined fatty acids (glycerides and sterides) by the same short alcohol, three successive distillations make it possible to successively recover a first fraction of hydrocarbons, the main fraction of alkyl esters, then the heaviest alkyl esters with squalene. The third distillate will be used for the production of squalene and a second fraction of hydrocarbons. The residue of the third distillation will be used for the production of sterols and vitamin E. By using bioethanol, plant-based glycerol and the plant-based hydrocarbons of the process, the process makes it possible to extract each of the four unsaponifiable substances without any solvent of oil-based origin and to claim the seals of approval for products obtained by natural physical and chemical processes.

Pheromone compositions based on a combination of squalene, linoleic acid and 1-docosanol are described. The compositions are useful for behavior modification in mammals that exhibit undesirable or harmful stress-related behaviors.

The invention provides zero trans-fatty acid multi-vitamin first-grade soybean oil and a preparation method thereof. The zero trans-fatty acid multi-vitamin first-grade soybean oil is produced by screening of soybean raw materials, a soybean peeling and instantaneous high-pressure wet-hot enzyme inactivation technology, a double-enzyme degumming / super degumming and washing-free long-time mixing and de-acidifying coupling technology, a two-step decolorization technology with low-activity decolorizing agent, a low-temperature short-time / two-stage catching, refluxing and deodorization technology, a ''nitrogen charging fresh-keeping'' technology and the like. In the zero trans-fatty acid multi-vitamin first-grade soybean oil, the trans-fatty acid content is less than or equal to 0.5 percent, the natural vitamin E content is more than or equal to 1,200ppm, the plant sterol content is more than or equal to 2,200ppm, and the squalene content is more than or equal to 60ppm; the soybean oil is packed by charging nitrogen, and any chemically synthesized antioxidant is not added; and compared with the commercially available first-grade soybean oil, the soybean oil is more safe, nutritional and healthy.

The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

The invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration. The composition comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity. In a preferred embodiment, the adjuvant mixture comprises an acrylic acid polymer, most preferably Carbopol, and a mixture of a metabolizable oil such as one or more unsaturated terpene hydrocarbons, preferably squalene or squalane, and a polyoxyethylene-polypropylene block copolymer such as Pluronic®. The vaccine composition may optionally include a preservative, preferably thimerosol and / or EDTA. In another emodiment, the invention provides an improved Mycoplasma hyopneumoniae bacterin vaccine composition, which advantageously provides immunity from infection after a single administration, and comprises an inactivated Mycoplasma hyopneumoniae bacterin and an adjuvant or adjuvant mixture, which, in combination, provide immunity from Mycoplasma hyopneumoniae infection after a single administration, and elicit an immune response specific to Mycoplasma hyopneumoniae bacterin and including cell-mediated immunity and local (secretory IgA) immunity, in combination with other vaccine components.

The invention relates to the technical field of vegetable-oil soft capsules, in particular to a peony-seed-oil soft capsule and a preparation method thereof. The peony-seed-oil soft capsule is prepared by mixing and gelling of raw materials, capsule pressing and forming, hardening, sizing, scrubbing and drying. The peony-seed-oil soft capsule comprises the following raw materials in parts by weight: 80-95 parts of peony seed oil, 1-3 parts of fucosterol, 1-3 parts of squalene, 1-3 parts of propyl gallate, 0.05-0.25 part of tocopherol, 0.05-0.25 part of soya bean lecithin, 0.15-0.25 part of vitamin C, 5-10 parts of gelatin, 5-10 parts of water, 1.3-2.5 parts of sorbierite, and 2.5-5 parts of glycerol. The peony-seed-oil soft capsule is nutritious and safe, has the obvious effects of reducing blood fat, blood pressure and cholesterol, and can enhance the self immunity, prevent the diabetes, prevent and treat cancers, reduce weight, prevent cerebral stroke and myocardial infarction, clear harmful substances in blood, prevent and treat heart diseases and relieve the climacteric syndrome.

The present invention provides a dry cleaning process for the in-home dry cleaning of laundry articles, comprising one or more cleaning steps followed by one or more rinse steps whereby at least one rinse step comprises contacting the laundry article with a rinse composition, said rinse composition comprising a low grade non-flammable non-chlorine containing organic dry cleaning solvent and said low grade solvent comprising at least 0.1 ppm and less than 50 000 ppm of squalene.

The invention describes a global method for extracting sterols, vitamin E, squalene and other vegetable hydrocarbons from deodorization distillates of vegetable oils. After esterification of the free fatty acids, followed by trans-esterification of the combined fatty acids (glycerides and sterides) with the same short alcohol, three successive distillations allow successive recovery of a first fraction of the hydrocarbons, the main fraction of alkyl esters, and then the heaviest alkyl esters with squalene. The third distillate will be used for producing squalene and a second fraction of hydrocarbons. The residue of the third distillation will be used for producing sterols and vitamin E. By using bio-ethanol, vegetable glycerol and the vegetable hydrocarbons of the method, with the method it is possible to extract each of the four unsaponifiables without any solvent of petroleum origin and claim the labels of products obtained by natural physical and chemical methods.

The invention relates to vegetable oil products, including palm oil products, containing one or more constituents from a group which includes monoacylglycerols, diacylglycerols, triacylglycerols, carotenes, tocols, phytosterols, squalene and free fatty acids, from vegetable oil sources and a process for the production thereof by using supercritical fluid extraction.

The present invention provides process of extracting squalene from Weiju eucalyptus, and the process includes the steps of preparing material, extraction, separation and purification. The process has low cost, convenient operation and high yield, and can utilize the rich Weiju eucalyptus resource to meet the industrial need and extend the industrial chain of timber industry and papermaking industry.

The invention discloses a method for extracting squalene by using camellia oleosa seeds as raw materials and tea oil and squalene soft capsules and relates to the technical field of biomedicine. The extraction method comprises the following process flows of material preparation, grinding, leaching, concentration, methyl esterification, complexant-borax reaction, macroporous resin chromatographic separation and purification, concentration, extraction, washing, concentration, preparation of squalene crude oil and supercritical carbon dioxide extraction, so that squalene essential oil with the squalene content of more than or equal to 80 percent is obtained. The tea oil and squalene soft capsules are prepared from the following raw materials in percentage by weight: 87 to 90 percent of squalene essential oil with the squalene content of more than or equal to 80 percent, 3 to 5 percent of polyethylene glycol 400, 2 to 3 percent of beewax and 2 to 5 percent of phospholipid. The raw material resources are wide. The prepared tea oil and squalene soft capsules not only can be used for medicine, but also can be used for food therapy, are particularly suitable for administration of treatment and food therapy of patients suffering from hepatitis, fatty liver, acute and chronic inflammation, tumor, chemotherapy, diabetes mellitus and cardio-cerebrovascular disease and have no toxic or side effects.

The invention provides a method for extracting squalene in vegetable oil deodorizer distillate through two-stage column chromatography, which comprises the following processing steps: performing saponifying pretreatment to separate unsaponifiable matters, performing first-stage column chromatography to separate tocopherol and sterol, and performing second-stage column chromatography to purify squalene. Through the whole process, the squalene having a content of about 50% can be obtained, and meanwhile, the tocopherol having a content of 70% or above and the sterol having a content of 98% or above can be also obtained. Thus, the method provided by the invention overcomes the problem of low pertinence in the traditional squalene extraction process, achieves favorable selectivity of column chromatography and greatly increases the extraction efficiency. The process has the advantages of simple operation procedure, high separation efficiency, high squalene recovery rate, low investment and the like.

Disclosed is a process for the recovery of a phytolipid composition from a vegetable oil by-product. The phytolipid composition produced comprises squalene, phytosterols, mixed tocopherols and tocotrieneols, and vegetable wax and is useful as an emollient. The phytochemical composition may be applied directly to the skin to provide emolliency. Alternatively, the phytolipid composition may be formulated in various aqueous or anhydrous cosmetic compositions such as creams, lotions, gels, ointments, lip balms, sticks, or pencils for treatment of the skin and lips. The phytolipid composition also may be incorporated into foods, beverages, and nutraceuticals to provide health benefits.

The invention relates to a skate liver oil and a preparation method thereof. The skate liver oil is characterized in that the skate liver oil comprises 0.76-1.13mass% of alkoxy glycerin, 0.71-0.92mass% of squalene, 25-28mass% of EPA and DHA, 1200-1600IU / g of vitamin A, 120-150IU / g of vitamin D, and the other substances which are lipids. The preparation method comprises the following steps: freezing skate livers, unfreezing, chopping, carrying out enzymatic hydrolysis treatment, carrying out enzyme killing treatment after the enzymatic hydrolysis, centrifuging, freezing the obtained emulsion, unfreezing, demulsifying, centrifuging, refining, degumming by citric acid, deacidifying by a caustic soda-soda ash mixture (a mass ratio of caustic soda to soda ash is 2:1), carrying out low temperature treatment, and carrying out evacuating nitrogen-filling packaging. The method has the advantages of reasonable operation, low cost and mild conditions, and the obtained skate liver oil has a very low peroxide value, a very low acid value and no nutrition loss, is rich in vitamin A, vitamin D, EPA, DHA, and important squalene and alkoxy glycerin, has a reinforced healthcare effect, and is easy to preserve and circuit in the market because of the evacuating nitrogen-filling packaging.

The invention discloses a tea oil processing method which comprises the following steps of: (1) carrying out pretreatment on raw material: drying tea seeds at low temperature, and then removing shells of the tea seeds; (2) preparing crude oil of the tea oil, and filtering; (3) carrying out supercritical CO2 refining: putting the crude oil of the tea oil into a supercritical CO2 device, extracting under the conditions of 15-25MPa and 30-60 DEG C; removing the impurities in the crude oil of the tea oil to obtain the tea oil finished product. The method adopts supercritical CO2 to remove the impurities of the crude tea oil, thus improving the quality of the tea oil, reducing the refining process, shortening the production cycle, reducing the pollution discharge, reducing the loss of nutritional ingredients of the tea oil in the refining process, increasing the content of active substances such as vitamin E and squalene, and improving the quality and the health care effect of the tea oil. The invention also discloses a device special for the method.

The invention discloses recombinant saccharomyces cerevisiae for producing dammarenediol and protopanoxadiol using xylose and a construction method. The construction method comprises the steps of replacing a promoter of a saccharomyces cerevisiae xylulokinase gene XKS1 with a promoter PFBA1 by virtue of a homologous recombination method, introducing xylose reductase XYL1 and a xylitol dehydrogenase XYL2 expression cassette, increasing the activities of transketolase TKL1 and transaldolase TAL1 so as to obtain recombinant bacteria 1, introducing a farnesyl-diphosphate farnesyltransferase gene ERG9, a squalene monooxygenase gene ERG1 and a dammarenediol synthase gene DS into the recombinant bacteria 1 so as to obtain recombinant bacteria 2, and introducing a nicotinamide adenine dinucleotide-hydroxymethylglutaryl coenzyme A reductase gene NADH-HMGr, farnesyl diphosphatesynthase ERG20 and a protopanoxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 into the recombinant bacteria 2, so as to obtain recombinant bacteria 3. According to the recombinant saccharomyces cerevisiae, dammarenediol and protopanoxadiol can be artificially synthesized by virtue of xylose.

The present invention provides an isolation process of the pure sterols from the SODD by simple acid catalyzed esterification and the separation of the reagents by distillation and water washing followed by the crystallization of phytosterols and purification. Soybean oil deodorizer distillate (SODD) and distillate from other vegetable oil refining contain free sterols, in addition to steryl esters, tocopherols squalene and unknown compounds. The process is also applicable to other vegetable oil distillate containing more than 1% phytosterols in the free form. Phytosterols thus obtained is of high purity (95-99%) and high yield (80-90%) and contains β-sitosterol, Stigmasterol and campesterol.

The invention relates to the field of daily use chemicals and in particular to a moisturizing, skin-whitening and spot-fading plant extract composition. The moisturizing, skin-whitening and spot-fading plant extract composition provided by the invention is capable of achieving the effects of moisturizing, skin-whitening, spot-fading and acne-fading. The composition is capable of, through using combination of an antioxidant extract, lactobacillus ferment, a licorice root extract, and a grape seed extract, achieving the effects of efficient antioxidant, skin-whitening and spot-fading, effectively fading spots of periocular skin, effectively relieving eye keratosis pilaris, and effectively relieving new acne; the composition is capable of, through using squalene, hyaluronic acid and a tuber melanosporum extract, achieving the high moisturizing effect. The composition is capable of, through using the licorice root extract, the squalene and the tuber melanosporum extract, achieving the anti-allergic effect, even if acne skin can also use the composition.

The present invention discloses an oil-in-water emulsion containing no surfactant, which comprises a metabolizable oil phase, an aqueous phase and oil-water amphipathic solid particles dispersed in the aqueous phase and having biocompatibility, wherein the oil phase comprises squalene or / and tocol; the aqueous phase is any one selected from the group consisting of purified water, water for injection, glycerine aqueous solution, buffering salts aqueous solution or clinically usable transfusion, or the combination of at least two selected therefrom; the solid particles have an average particle size in a scale of from nanometer to micrometer. The emulsion of the present invention can be used as vaccine adjuvant, drug delivery carrier or sustained / controlled-release carrier, and have the controllable properties. The resultant emulsion is stable, avoids the use of surfactants and can reduce the human poisoning and environmental pollution.

The invention discloses a method for simultaneously determining stigmasterol, campesterol, beta-sitosterol and squalene in vegetable oil. The method comprises the following steps of: 1) saponifying the vegetable oil, and extracting an unsaponifiable matter in a saponification solution by using an organic solvent; and 2) dissolving the unsaponifiable matter in the organic solvent, and determining by using high-performance liquid chromatography, wherein the high-performance liquid chromatography has the chromatographic conditions that a chromatographic column is a C18 column, a mobile phase is a solvent of which the volume ratio of acetonitrile to water is (90:10) to (100:0), an ultraviolet detection wavelength is 205 to 210 nm, a sample size is 10 to 20 mu L, flow speed is 1.0 to 1.5 mL / min and column temperature is 25 to 30 DEG C. By adoption of the method, effective separation of the campesterol, the stigmasterol, the beta-sitosterol and the squalene in the vegetable oil can be simultaneously realized, consumed time for separation is about 40 min, and components in a chromatogram are eluted in a sequence of the campesterol, the stigmasterol, the beta-sitosterol and the squalene.

The invention discloses a plum extract containing 0.5-50wt% of squalene. The plum extract mainly comes from liposoluble effective parts and / or water-soluble effective parts from non-fruit part of plum. The invention also provides the preparation method of the plum extract, Liposoluble effective part is obtained through extracting of super-criticality CO2 fluid or nonpolarity organic solvent; the residue of the super-criticality fluid or nonpolarity solvent extract is extracted by alcohol-water solution to obtain the water-soluble effective part. The invention discloses the composition containing plum extract and also discloses application of the plum extract.

The invention relates to a method for preparing momordica grosvenori squalene, which comprises the following step: breaking kernels or seeds of the momordica grosvenoris, leaching the liposolubility substance by organic solvent, then removing the organic solvent in the liposolubility substance to get the crude momordica grosvenori squalene, getting raw momordica grosvenori squalene through a silica gel chromatography column and eluting by organic solvent, collecting the achromatic color part of the eluent, removing the organic solvent using vacuum distillation process to get the momordica grosvenori squalene super quality. The content of squalene is more than 40 % in the crude production and more than 95 % in the exquisite production. The production has the special perfume and can substitute completely squalene abstracted from the liver of abyssal sea sharks.