84results about How to "High in DHA" patented technology

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with the standard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The invention discloses a Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and a fermentation method thereof. The Schizochytrium sp.TIO1101 strain provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4603. After the Schizochytrium sp.TIO1101 is subjected to high-density heterotrophic fermentation for 72h, the biomass liveweight, the lipid content and the DHA are respectively 120g / L, 54.3% and 51.4% of the content of total lipid, therefore the Schizochytrium sp.TIO1101 has development and application values extremely. The Schizochytrium sp.TIO1101 disclosed by the invention has the advantages that: the Schizochytrium sp.TIO1101is obtained through separation; the high-density heterotrophic fermentation of the Schizochytrium sp.TIO1101 is realized; and the lipid extracted from the Schizochytrium sp.TIO1101 cells obtained under the fermentation condition has high DHA content and purer components.

The invention discloses a DHA algae oil ethyl ester and a preparation method thereof. The method comprises the following steps: carrying out ethyl-esterifying on crypthecodinium cohnii DHA grease and then purifying trough a secondary molecular distillation method to obtain high-purity DHA ethyl ester. The invention further discloses a DHA glyceride and a method for transferring the DHA algae oil ethyl ester into glyceride. The method comprises the following steps: mixing the DHA algae oil ethyl ester with glycerinum in a material tank, feeding the mixed material in a reactor having immobilized lipase, performing transesterification to replace out the ethanol, recycling through a condenser, and flowing the reacted mixture back to the material tank, circularly reacting for 5-60 hours, and finally returning to the material tank, wherein the glyceride product is obtained by performing molecule distillation on the material. The method disclosed by the invention is suitable for the industrial production, the DHA greased produced from the microorganism is used as the raw material, the percent content of the DHA is greatly improved, the ethyl ester is transferred into the glyceride, the ethanol is avoided, the side effect after eating is not generated, and the utilization degree of the DHA algae oil ethyl ester in vivo is effectively improved.

The invention discloses a docosahexaenoic acid-producing bacterium and a mutation and screening method and application thereof. The strain is classified and named as Crypthecodinium cohnii HX-308, is preserved in China Center for Type Culture Collection, and has the preservation number M208246. The strain is a DHA-producing bacterium with high yield, has the DHA content 1.53 times of a starting strain, stable physiological-biochemical characteristic and stable heritability, and simultaneously can produce polyunsaturated fatty acid through liquid culture and fermentation; the content of unsaturated fatty acid reaches 20.4g / L, wherein the DHA content reaches 8.16g / L; and the strain is a good strain for producing natural DHA, and has excellent industrial production prospect.

The invention relates to a preparation and separation method of docosapentaenoic acid methyl ester and docosahexaenoic acid methyl ester. Microalgae oil is used as raw material to prepare docosapentaenoic acid and docosahexaenoic acid which are mixed with fatty acid to prepare the docosapentaenoic acid methyl ester and the docosahexaenoic acid methyl ester. The separation method of the the docosapentaenoic acid methyl ester and the docosahexaenoic acid methyl ester provided by the method adopts a silver nitrate column method of separating. In the silver nitrate column method, the quality of the methyl ester sample introduction of the mixed fatty acid is 0.5 percent to 10 percent of the quality of filled silver nitrate blangel; elution gradient is n-hexane solution containing acetone the content of which is 0.5 percent to 15 percent; the elution speed is 0.5 to 5ml / min. The preparation and separation method utilizes the silver nitrate column method to obtain the high purity docosapentaenoic acid methyl ester and the high purity docosahexaenoic acid methyl ester, wherein, the content of DPA is raised from 20.32 percent in raw oil to 99.61 percent, the content of DHA is raised from 44.83 percent to 99.02 percent, and the yield rate by using the silver nitrate column method can reach more than 90 percent.

The invention relates to a ginkgo biloba extract and fish oil soft capsule. Memory deterioration is one of body function debilitating symptoms of elders, the memory deterioration commonly occurs in youngsters and adults with the increasingly fierce social competition, and chemotherapy has a by-effect. The ginkgo biloba extract and fish oil soft capsule content is prepared by tuna oil, ginkgo biloba extract, natural vitamin E, beewax and soybean oil according to a proportion of parts by weight of (432-528):(135-165):(0.63-0.77):(40.95-50.05):(21.42-26.18). The invention further relates to a preparation of ginkgo biloba extract and fish oil soft capsule. The ginkgo biloba extract and fish oil soft capsule provided by the invention can be used for effectively improving the intracerebral DHA content, optimizing the brain tissue ingredients, improving the brain blood oxygen content and perfecting the memory, thereby being suitable for people of all agents and particularly suitable for elders with memory deterioration and brain function decline.

The invention provides a fermentation production technique of schizogenesis chytrid, which solves the problems of lower yield and higher production cost in the prior art. The fermentation production technique has the technical scheme that bean pulp is taken as main nitrogen source for fermentation production, and the technique comprises the steps of bean pulp hydrolysis, pressing filter, preparation of fermentation medium, arrangement of a fermentation tank system, collection and drying of the schizogenesis chytrid, and preparing the schizogenesis chytrid dry powder. The new fermentation production technique of the schizogenesis chytrid takes the bean pulp as main nitrogen, thus not only reducing the cost of fermentation production, but also improving the growth rate of the schizogenesis chytrid and promoting fatty acid accumulation of the cells of the schizogenesis chytrid.

The invention discloses microalgae whole cell powder for making mammals produce DHA milk in high yield. The microalgae whole cell powder is prepared from raw materials of microalgae DHA cell pulp, an emulsifying agent, an antioxidant, a filler, a packing material, a dispersing agent and water. The preparation method comprises the following steps of: preparing the microalgae DHA cell pulp, and preparing an aqueous phase and a pulp phase; and mixing, emulsifying, homogenizing, spraying and premixing the aqueous phase and the pulp phase, and pelletizing to prepare the microalgae whole cell powder. The microalgae whole cell powder keeps the completeness thereof, is more easily absorbed by the mammals, and effectively keeps DHA components in the milk. Therefore, the DHA content of the finally secreted milk is much higher than that of common milk, the intake demands of all kinds of people on the DHA are met, the property is stable, and the biological functions of the DHA are fully exerted.

The invention discloses DHA gelatin candies and a making method thereof. The gelatin candies comprise the following components in parts by weight: 50-65 parts of sandwich and 25-40 parts of gelatinized coatings, wherein the sandwich comprises the following components in parts by weight of 10-15 parts of algae oil DHA, 0.8-1.2 parts of phosphatidylserine, 0.3-0.6 part of vitamin A, 0.3-0.6 part ofvitamin B, 0.3-0.6 part of vitamin E and 34.3-40 parts of sugar alcohol; and the gelatinized coatings comprise the following components in parts by weight: 8-12 parts of gelata, 16-26 parts of sugar alcohol and 1-2 parts of a flavoring agent. The making method of the DHA gelatin candies comprises the following steps of making the sandwich according to the quantity of the formula; making the gelatinized coatings according to the quantity of the formula; and making the sandwich gelatin candies. The DHA gelatin candies disclosed by the invention are free from fishy smell, good in mouth feel and high in DHA content; and through sandwich design, DHA and the like can be isolated from air, and the DHA gelatin candies are good in stability and can be stored for a long term. In addition, accordingto the DHA gelatin candies disclosed by the invention, the DHA, the phosphatidylserine, the vitamin A, the vitamin B and the vitamin E are compounded for use, so that the DHA gelatin candies has positive effect on preventing Alzheimer's diseases, the made DHA gelatin candies are also quite suitable for old people to eat, and the defect that most of conventional products in the market are designedfor babies and pregnant women is overcome.

The invention relates to a microbiological feed additive and a preparation method and an application thereof, relating to a feed additive. The invention provides a microbiological feed additive compounded with various microbiological fermented powders and a preparation method and an application thereof. The microbiological feed additive consists of the following compounds according to weight percentages: 60%-99% of DHA (Docosahexenoic acid) fermented powder, 0-25% of AA (Arachidonic acid) fermented powder and 1%-30% of R. spkaeroides fermented powder. After the nutrition compositions are mixed uniformly after being crashed respectively; and then sieving, checking, weighing and packing are performed. The microbiological feed additive can be used as an active in aquatic product, poultry and livestock cultivation feeds, and the addition accounts for 0.2%-5.0% of total mass of basal feed. The contents of the nutrition compositions in the feed are increased, so that the resistance against diseases, survival rate, growth rate and nutritive value of fed animals are improved; rich coenzyme Q10 and carotenoid are used as antioxidants, so that the oxygenization of polyunsaturated fatty acid can be inhibited, the stability and using effect of polyunsaturated fatty acid are improved, and the compounding effect is obvious.

The invention discloses a feed additive for improving docosahexaenoic acid (DHA) conversion efficiency and content in poultry egg, and application thereof. The feed additive comprises ethanol extract of leaves of genus Linum plants obtained by reflux extraction with 70%-100% ethanol. The feed additive can promote conversion efficiency from alpha-linolenic acid to DHA, improve DHA content in egg yolk, and reduce eicosapentaenoic acid (EPA) content. The feed additive can be applied in production of high-DHA poultry eggs, and provide material for DHA egg yolk powder and DHA egg yolk powder-enhanced food, which can be served for long term with high safety and no risk.

The invention discloses a dehydroacetic acid (DHA) feed additive prepared by fermenting kitchen waste and a preparation method thereof. The DHA feed additive is prepared by carrying out spray drying on a fermentation solution of a DHA-producing strain; and the DHA content in the DHA feed additive is higher. The preparation method comprises the following steps: carrying out pretreatment and sectional enzymolysis on the kitchen waste to obtain an enzymolysis composite, and centrifugating the enzymolysis composite to obtain a precipitate which can be made into feed albumen powder and an enzymolysis supernatant which can be made into a fermentation culture medium; autoclaving, and inoculating the DHA-producing strain to perform fermentation, thereby obtaining the fermentation solution; and adding an antioxidant and / or a flocculant into the fermentation solution to obtain the DHA feed additive. The kitchen waste enzymolysis solution can be simultaneously substituted for the carbon source and nitrogen source in the culture medium, thereby lowering the production cost of the culture medium, further greatly lowering the production cost of the DHA feed additive, and implementing the maximized utilization of the kitchen waste resources.

The invention provides a DHA fermenting high-yielding method. The DHA fermenting high-yielding method includes the steps that schizochytrium limacinum strains are domesticated through a culture medium with a strong oxidant, the domesticated strains are cultured through a fermenting culture medium, and fermenting high yielding of DHA can be achieved accordingly. After the domesticated strains are fermented, the DHA content is 57.9%, and oil-producing efficiency is 0.492 g / L / h; compared with a control group, the DHA content and the oil-producing efficiency are increased about 20% and 55% respectively. The domesticating method does not involve a genetic engineering method, does not harm the environment, does not increase manpower and material resources, is simple and convenient, and has economic benefits.

The invention discloses a DHA regulating agent and a chicken feed applying the DHA regulating agent. The DHA regulating agent comprises the following ingredients in parts by weight: 3-5 parts of hazelnut powder, 5-20 parts of peanut powder, 8-30 parts of sesame, 8-25 parts of almond powder, 12-40 parts of schizochytrium powder, 14-23 parts of sunflower seed and 3-15 parts of flaxseed. The DHA regulating agent and the chicken feed applying the DHA regulating agent have the advantages that DHA content in eggs can be increased, quality of the eggs also can be improved, the DHA regulating agent in the whole production process is safe and healthy, no antibiotic or hormone is contained, no heavy metal pollution is generated, and blank in a domestic technology is filled up.

The invention discloses application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants. The preservation number of the schizochytrium limacinum atChina General Microbiological Culture Collection Center is CGMCC No. 13746. By schizochytrium limacinum powder prepared from the schizochytrium limacinum of the invention, the content of DHA in milkof the ruminants can be increased. The natural-source high-DHA-content milk is organic, safe and stable, is easy to absorb, and is safe and effective for people to take natural DHA, demands of consumers can be catered and met, and thus, the schizochytrium limacinum and the schizochytrium limacinum powder can be widely applied to the field of common food and the field of livestock breeding.

The invention discloses a preparation method of algal oil DHA. The preparation method comprises following steps: 1, selected microalgae is subjected to algae seed culturing and heterotrophic fermenting so as to obtain a microalgae raw material; 2, the microalgae raw material is subjected to drying treatment so as to obtain microalgae powder; 3, the microalgae powder is subjected to microalgae wallbreaking pre-treatment so as to obtain dried microalgae powder; 4, algal oil is extracted, wherein the dried microalgae powder is taken as a raw material, an ethanol solution with a mass concentration of 60 to 100% is taken as an extractant, an accelerated solvent extraction instrument is adopted for extraction, an obtained extract is introduced into a vacuum rotatory evaporator for organic solvent evaporation, and a residue obtained through evaporation is the algal oil; 5, urea coating enrichment purifying is carried out; and 6, filtering condensation is carried out so as to obtain algal oilDHA finished products. The product purity of the preparation method is high, defects in preparation of DHA from fish oil and microalgae are avoided, and the finished product can be taken as a raw material in the fields of food, health care product, food additive, and high grade chemical product.

The invention provides a feed functional package for producing pork rich in DHA and EPA, feed and an application method thereof. The feed functional package for producing the pork rich in DHA and EPA comprises the following components: seaweed powder and flaxseed. For optimizing and improving the edibility and functionality of the pork, algae and the flaxseed are applied to compounding and development of the feed for producing the pork rich in DHA and EPA, so that the content ratio of DHA to EPA in the produced pork is more scientific, the produced pork has no fishy taste or residues such as heavy metals, and the problems are solved; meanwhile, a feeding method of the feed functional package is optimized, so as to improve the composition of fatty acids of the pork; and the feed functional package provided by the invention has great significance on human body fatty acid equilibrium.

The present invention provides a feed rich in docosahexaenoic acid (DHA). The feed is formed by uniformly stirring base feed and algal meal containing DHA according to a certain proportion, wherein the base feed comprises corn, soybean meal, dicalcium phosphate, stone powder, salt, multi-vitamin premix and lysine, and the algal meal is formed by subjecting by-products in micro-organism oil production process to drying and grinding. The resources of the feed are wide, the cost is low, the preparation method is easy and convenient to operate, the feed does not contain harmful components, the content of DHA in livestock and poultry products is increased, and the feed is suitable for large-scale production and application.

The invention discloses high-nutrient laying hen feed. The high-nutrient laying hen feed contains a plurality of types of nutrient substances including energy, protein, amino acids, vitamins and mineral substances, namely corns, fish meal, seaweed meal, soybean meal, blood powder, bone meal, mountain flour and table salt. According to the high-nutrient laying hen feed, the content of high-quality protein is improved; the seaweed meal is added and all-natural raw materials are adopted so that the protein is relatively optimized; after the test and checking of Beijing Nutrition Research Institution, compared with common eggs, components of unsaturated fatty acids of eggs laid by laying hens eating the feed are increased to 13 types from 5 types and the total amount is increased to 9.46 from 4.65; particularly, the content of DNA beneficial to improvement of intelligence and health of hearts and brains is increased to 0.63 gram per 100 grams from 0.04 gram per 100 grams and is increased by 15.75 times. Furthermore, yolks are full and egg white is thick; the eggs are orange red when the eggs are not cooked, and are golden yellow after being cooked; the eggs have a good-looking appearance and a fragrant mouth feel; and the eggs are sold locally and in cities including Beijing, Shanghai, Tianjin, Shenzhen and the like, and are profoundly popular with consumers.

The invention belongs to the technical field of algae and discloses a method of increasing the biomass and DHA productivity of isochrysis galbana parke. The method includes: S1) inoculating the isochrysis galbana parke, which has been cultured to logarithmic phase, on a seed pot filled with a seed culture medium, and culturing the isochrysis galbana parke for 48 h and collecting a seed liquid; S2)inoculating the seed liquid on a reaction tank filled with a fermenting culture liquid, performing fermenting culture at 22-25 DEG C, when the cultivation reaches 48 h, adding arachidonic acid and propyl gallate to the fermenting culture liquid and continuously culturing the seed liquid for 72-96 h through fermentation, and collecting algae cells. The method can increase the biomass and DHA productivity of the isochrysis galbana parke.

The invention discloses a microbial oil and an extraction method thereof, belonging to the field of extraction of microbial oil. The extraction method comprises the following steps: (1) contacting a fermentation broth of an oil producing microorganism with a cell wall lyase in an aseptic environment to perform enzymolysis on the oil producing microorganism in the fermentation broth so as to obtaina mixed solution containing the microbial oil; and (2) subjecting the mixed solution containing the microbial oil obtained in the step (1) to oil-water-solid three-phase separation so as to obtain the microbial oil. With such a technical scheme, the whole process is controlled to be implemented in the aseptic environment, contaminative microbes are prevented in the extraction process of the microbial oil, foreign bacteria and toxins are prevented from migrating into the product, the safety of the microbial oil can be increased, and the yield of the obtained microbial oil used as crude oil canbe increased to 97-98%, which is significantly improved compared with the same industry; in addition, the DHA content or ARA content of the crude oil is significantly improved, and the content of extracellular toxins is significantly reduced.

The invention belongs to the technical field of the production of dairy products, and particularly relates to a production method of purely natural DHA (docosa hexaenoic acid) raw milk and an application of the production method. According to the production method of purely natural DHA raw milk, a ration with schizochytrium limacinum powder is fed to cows at the lactation period, so that the content of DHA ingested by the cows is increased; and through autologous metabolism of the cows, natural DHA fresh raw milk stable in production state is transformed. The raw milk has processing characteristics of normal raw milk, and besides, contains certain natural DHA components, in the processing course of subsequent dairy products, later-stage addition of DHA nutrient components is not needed, the problems that peculiar smell is easy to produce in the addition course of product ingredients and the processing technology is complex are solved, and a novel way is opened up for production of purely natural DHA raw milk and dairy products.

The present invention provides a feed for producing pork containing DHA and a use method thereof. The feed comprises corns, soybean meal, wheat bran, lysine, methionine, stone powder, calcium formate, calcium hydrogen phosphate, edible salt, a premix for pigs, and schizochytrium limacinum powder or chlorella powder. The method aims at applying alga to develop the feed for producing the pork enriched in DHA. The DHA in the alga is not subjected to a food chain transmission and the feed does not contain heavy metals, is safe, pollution-free, high in content and free of fish smell, replaces fish oil, and solves the problems in the prior art. Besides, after feeding, the DHA content in the fat of longissimus dorsi of the produced pork is 40% or more higher than that of common pork, the content of the DHA is higher than the content of EPA, and a ratio of omega-6 to omega-3 is lower than 2.8. At the same time, after feeding, the degree of oxidation of the produced pork is not significantly different from that of the common pork. The optimized feeding method effectively improves the fatty acid composition of the pork and the produced pork is of great significance to the fatty acid balance of human body.

The invention discloses a laying hen daily ration capable of improving quality of eggs. The laying hen daily ration contains thraustochytrium powder. A method for fermenting the thraustochytrium comprises the following steps; (1) culturing seeds, namely, inoculating thraustochytrium spores in a seed culture medium to activate; (2) fermenting to culture, namely, inoculating the activated seeds into a fermentation tank for fermentation and culture, after being cultured for 36-60 hours, adding 1-4% cocinic acid into the fermentation tank in a uniform speed so as to increase the DHA (Docosahexaenoic Acid) content, wherein the temperature within 84-108 hours in the front culture period is 22-25 DEG C, and the temperature in the later period is 28-30 DEG C for inhibiting generation of EPA (Timnodonic Acid). Due to addition of the dry thraustochytrium powder into the daily ration, the DHA content of eggs is directly increased, the DHA deposition velocity is fast, the time for enriching and stabilizing the DHA in eggs is shortened, and the eggs are free of EPA and thus are applicable to more people.

The invention discloses a preparation method of schizochytrium limacinum powder. The preparation method comprises the following steps: mixing schizochytrium limacinum thalli with a wall material, an emulsifier and an antioxidant, stirring, shearing and homogenizing to obtain a suspension; drying the obtained suspension to obtain schizochytrium limacinum powder of which the water content is less than 5%; transferring the obtained dry powder into a coating machine, and spraying a coating solution prepared from a coating agent at 30-40 DEG C to obtain schizochytrium limacinum powder; wherein theschizochytrium limacinum thallus is obtained by performing acid treatment or alkali treatment or wall-breaking pretreatment of biological enzyme treatment on fermentation liquor after schizochytrium limacinum strain fermentation. The prepared schizochytrium limacinum powder is used for feeding animals, can be prevented from being degraded in rumens of the animals, and can be absorbed by the animals more easily after passing through the rumens, so that milk of the animals is rich in DHA.

The invention provides a method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid (DHA). The method comprises the following steps: with a heterotrophic culture medium of crypthecodinium cohnii as a foundation, adding any chemical promoter into the heterotrophic culture medium; the heterotrophic culture medium is composed of 9g / L of glucose, 2g / L of yeast extract powder and 25g / L of sea salt; the chemical promoter can also be called a plant growth regulator, including indoleacetic acid (IAA), naphthoxyessigsaeure (BNOA), chlorobenzoic acid, salicylic acid (SA), abscisic acid (ABA) and cholamine, and the concentrations of these chemical promoters in the heterotrophic culture medium are as follows: 2.5mg / L of indoleacetic acid (IAA), 4mg / L of naphthoxyessigsaeure (BNOA), 4mg / L of chlorobenzoic acid, 1mg / L of salicylic acid (SA), 20mg / L of abscisic acid (ABA) and 2.5mM cholamine; culturing at 25 DEG C for 72 hours, extracting grease, and measuring the DHA content of the culture by using a gas chromatograph-mass spectrometer, meanwhile, extracting cellular metabolite from a parallel culture, and detecting and analyzing the content change condition of the cellular metabolite by using a metabonomics method.