The present invention discloses a
microorganism DHA
nutrition oil (
docosahexaenoic acid)
fermentation production method. According to the method, bacterial
Schizochytrium sp. is inoculated into a
shake flask to culture, then is inoculated into a seed tank to carry out scale-up culture, and finally is inoculated into a
fermentation tank to carry out two-step
fermentation, wherein in the first step, a temperature is controlled to 25-30 DEG C, the pH value is 6, a fermentation culture medium comprises glucose,
yeast extract paste, peptone and an inorganic salt, and fermentation is performed for 2-3 days, in the second step, cooling is performed to a temperature of 20-25 DEG C,
continuous fermentation is performed for 2-3 days, a concentration of the glucose is always controlled to 20% during the fermentation process by additionally supplying the glucose, the amount of microorganisms prepared through fermentation is about 60 g / L,
oil content in dry
biomass is 50-60%, DHA content is 35-40%, and DHA productivity is 8-10 g / L.day. The
microorganism DHA fermentation production technology has characteristics of high DHA yield, high purity, stable production technology, simpleness and low cost.