The invention discloses a method of producing a
protein feed by a liquid-
solid two-step
fermentation method. The method comprises the following steps: performing
liquid culture on
corynebacterium glutamicum, candida utilis,
bacillus subtilis,
lactobacillus plantarum and
aspergillus oryzae so as to obtain
corynebacterium glutamicum
fermentation liquor, candida utilis
fermentation liquor,
bacillus subtilis fermentation liquor,
lactobacillus plantarum fermentation liquor and
aspergillus oryzae fermentation liquor; mixing the
corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the
bacillus subtilis fermentation liquor, the
lactobacillus plantarum fermentation liquor and the
aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of
soybean meal, cluster bean
meal,
cottonseed meal and the like into an explosion vat for performing
steam explosion; after
steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an
enzyme preparation so as to obtain
mixed materials; conveying the
mixed materials into a special modularized
solid-state biological
raw material continuous fermentation device for enzymolysis and fermentation, and
drying the fermented materials so as to obtain the
protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the
enzyme preparation are mixed, then the
mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic
temperature control, automatic dampness control and automatic
ventilation control are met, the
bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.