The invention discloses a Chinese medicine composition for prevention and control of porcine circovirus disease and a preparation method thereof, and belongs to the field of traditional Chinese medicine for livestock. The Chinese medicine composition is composed of twenty traditional Chinese medicines comprising honeysuckle flower, bupleurum, Herba Houttuyniae, Radix Isatidis, Radix Astragali, licorice, fructus forsythiae, wolfberry fruit, dried tangerine peel, baikal skullcap root, epimedium, bighead atractylodes rhizome, cucumber, rhizoma atractylodis, Folium Isatidis, Polyporus umbellatus, angelica, Codonopsis pilosula, Poria and Rhizoma Coptidis. An injection is prepared by steps of crushing, decoction, enzymolysis, flocculation precipitation, concentration, filtration, sealing and sterilization. The pure natural herbs adopted by the invention are safe, reliable and without toxic or side effects; compatibility of the medicines is in line with the principle of assistant and guide; and the composition is safe, and has no toxic or side effects, and the efficacies of clearing heat, removing toxicity, resisting bacteria, relieving inflammation, and enhancing immunity of the body. The prepared injection has high bioavailability and stable traits, can effectively enhance immunity of the body, and has good treatment effect on porcine circovirus disease.