The invention discloses a liquid
chromatography-
tandem mass spectrometry method used for simultaneous detection of 10 kinds 94 residual drugs in
livestock and poultry. The liquid
chromatography-tandemmass spectrometry method used for simultaneous detection of 10 kinds 94 residual drugs in
livestock and poultry comprises following steps:
sample preparation, wherein
livestock and
poultry meat is prepared into
meat paste so as to obtain samples to be measured; extraction
solvent preparation, wherein,
acetonitrile, water, and
formic acid are mixed to be uniform so as to obtain the extraction
solvent;
sample extraction, wherein the samples to be measured are weighed, an internal reference working
mixed solution is added; homogenizing is carried out after adding of the extraction
solvent,
centrifugation is carried out, a supernatant liquid 1 is collected; and sample purifying, wherein the solution to be tested is subjected to extraction using an Oasis PriMe HLB
solid phase extraction column, the extraction solvent is adopted to washing the
solid phase extraction column, and obtained eluants are mixed;
nitrogen blowing is carried out, an obtained mixture is diluted to a constant volume,
centrifugation is carried out, an obtained supernatant 2 is collected, and filtered so as to obtain a solution to be tested; and measuring, wherein the solution to be tested is subjected to UPLC-MS / MSmeasuring. The liquid
chromatography-
tandem mass spectrometry method is capable of realizing simultaneous detection of 10 kinds 94 residual drugs in 15min, detection efficiency is increased greatly,labor cost is reduced greatly, the reagents are saved, and the liquid chromatography-
tandem mass spectrometry method is green, and is friendly to the environment.