The invention relates to a preparation method of
mucus protein peptide of
andrias davidianus. The preparation method comprises the steps that 1,
mucus of the
andrias davidianus is collected through an
electric shock stimulus method, and freeze-dried to obtain freeze-dried
mucus powder; 2, the obtained freeze-dried mucus
powder is added to
distilled water with the
mass of 50-120 times, then
alkaline protease which is 10 percent-30 percent of the
mass of the freeze-dried mucus
powder is added, pH is adjusted to 9-9.5, enzymolysis is carried out for 1-3 h under the temperature condition of 40 DEG C-60 DEG C, and then mixed liquid is boiled for 1-2 min and cooled to 19 DEG C-25 DEG C; 3, the mixed liquid treated in the step 2 is centrifuged for 10-20 min under the condition of 3,000-4,500 r / min,
clear liquid is taken, the pH value of the
clear liquid is adjusted to 4-5, under the temperature condition of 40 DEG C-45 DEG C,
activated carbon of one percent to two percent is added, stirring is carried out for 45-60 min for decolorizing and deordorizing, and then under the condition of 3,000-4,500 r / min,
centrifugation is carried out for 10-15 min, and filter liquor is obtained through a filter film of 0.45
micrometer; 4, the filter liquor treated in the step 3 is concentrated to 1 / 8 -1 / 7 of an original sample under the temperature condition of 30 DEG C-60 DEG C in a rotary
evaporation mode, concentrated liquor is sealed with a
plastic bag, sterilization is carried out for 1-5 min under the conditions that temperature ranges from 19 DEG C to 25 DEG C and pressure ranges from 150 MPa to 250 MPa, and vacuum freeze-
drying and smashing are carried out in clear environment to obtain mucus
protein peptide powder.