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Andrias davidianus collagen glycopeptide and application thereof

A giant salamander, glycopeptide technology, applied in the direction of peptides, specific peptides, connective tissue peptides, etc., can solve problems such as giant salamander collagen glycopeptides that have not yet been seen

Active Publication Date: 2016-03-16
张家界金驰天问农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there have been no relevant reports on the production of giant salamander collagen glycopeptides and their biological activities from giant salamander collagen and oyster polysaccharides.

Method used

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  • Andrias davidianus collagen glycopeptide and application thereof
  • Andrias davidianus collagen glycopeptide and application thereof

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experiment example

[0023] 1. Utilizing β-racemization reaction to determine the connection mode of giant salamander collagen glycopeptide bond in the embodiment of the present invention

[0024] The β-racemization reaction is a common method to identify the connection mode of glycopeptide bonds. The O-glycopeptide bond is treated with alkali, and the ultraviolet absorption at 240nm wavelength is enhanced.

[0025] Accurately weigh 5 mg of the giant salamander collagen glycopeptide sample of the embodiment of the present invention, dissolve it in 3 mL of distilled water, and then divide the sample solution into two equal parts, each 1.5 mL. One part was added with 1.5mL distilled water; the other part was added with 1.5mL0.4mol / L NaOH solution. After reacting for 2 hours, the two solutions were scanned by UV spectrum in the range of 200-400nm respectively, and the UV scan pictures before and after the β-elimination reaction were obtained ( figure 1 ). Depend on figure 1 It can be seen that t...

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Abstract

The invention discloses Andrias davidianus collagen glycopeptide and an application thereof. The Andrias davidianus collagen glycopeptides is prepared according to the following method: Andrias davidianus skin is used as a raw material, and Andrias davidianus skin acid-soluble collagen is prepared; the Andrias davidianus skin acid-soluble collagen is used as a raw material, and Andrias davidianus skin collagen peptide is prepared; 10g of oyster polysaccharide is added into 100mL of 0.01mol / L sodium phosphate buffer whose pH value is 6.5, stirring is carried out for 2 hours, 5g of Andrias davidianus collagen peptide and 10g of oyster polysaccharide are added, stirring is continuously carried out for 2 hours, the solution is permitted to stand overnight at 4 DEG C, stirring is carried out for 4 hours at 60 DEG C, the solution is cooled to room temperature, centrifugation at a speed of 9000r / min is carried out for 30 minutes, a supernatant is obtained, the supernatant is frozen and dried to obtain the Andrias davidianus collagen glycopeptide, and the collagen glycopeptide can be applied to cosmetics and fruit juice beverages.

Description

technical field [0001] The invention relates to a giant salamander collagen glycopeptide prepared from giant salamander skin and oyster polysaccharide and its application. Background technique [0002] giant salamander ( Andrias davidianus ) is a rare specialty unique to our country and belongs to the second-class national protected animals. It is mainly distributed in streams in the middle and upper reaches of the Yangtze River, the middle and upper reaches of the Pearl River and the upper reaches of the Han River. Since the 1990s, Hunan, Hubei, Shaanxi and Guangxi have established a large number of giant salamander domestication and breeding enterprises. In Hunan Province alone, there are about 35 giant salamander domestication and breeding enterprises, with an annual reproductive capacity of 40,000 to 50,000. With the development of breeding scale, the intensive processing and utilization of giant salamander resources has become an urgent issue related to the sustainabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/107C12P21/06A61K8/65A61Q17/00A23L29/30A23L2/52
CPCA23L2/52A61K8/65A61Q17/00C07K14/78
Inventor 李伟佟长青王文莉
Owner 张家界金驰天问农业科技有限公司
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