2758 results about "Domestication" patented technology

The invention discloses a microbial agent for treating ammonia-containing waste water and a preparation method thereof. The microbial agent for treating ammonia-containing waste water mainly comprises three bacterial strains of nitrobacter sp, nitrosobacteria nitrosomonas sp., denitrobacter sp., and the contents of the three bacterial strains can be regulated or proportioned according to specificcultivation conditions and product requirements through methods of oriented domestication, and the like. The cultivation method comprises the following steps of: enriching a bacterial source, purifying and amplifying stage by stage for cultivating. The microbial agent can be used for oriented domestication according to a waste water treatment result so as to improve the content of a target bacterial strain. The cultivation method has the advantages of high cultivation efficiency, large production capacity, and the like and is suitable for commercially producing complex microorganisms for treating the ammonia-containing waste water in a large scale. The obtained microbial agent can effectively treat low-COD (Chemical Oxygen Demand) and high-ammonia-nitrogen waste water, such as waste water of catalyst production, and the like.

The present invention relates to a delivery platform that can be used to genetically modify a target in a plant or an alga. In one instance, polypeptides and / or polynucleotides can be delivered using silica delivery platforms, e.g., silica carriers or protocells. Such platforms can be employed to control gene activation and repression in the plant or alga.

The invention discloses a short-term and medium- and long-term electric power load prediction method based on machine learning model. Firstly, preprocessing is conducted on data, including smootheningabnormal data and filling missing data. Factors of affecting load changes will be analyzed, including historical data, time periodicity, and weather variable characteristics. Domestication will be conducted on all input variables for accelerating learning speed and raising prediction precision. The invention is advantageous in that linear regression is compared, and the performance of the vectorregression and gradient lifting regression in the short-term and medium- and long-term electric power load prediction is supported; with the prolongation of the prediction time, the performance of thegradient lifting regression model is better that that of the other two models; the AdaBoost algorithm which uses the gradient lifting tree as a basic classifier is brought forward, and load prediction is conducted, and the precision of electric power load prediction can be effectively raised.

The invention relates to a water circulation system and a method for breeding marine fishes inland, and mainly solves the technical problems of complex process, large investment, high operating cost, large limitation of breeding varieties, low survival rate, slow growth and the like in the process of breeding the marine fishes in fresh water or low salinity salt water at present. A technical scheme comprises the following steps of: a, establishing the water circulation system; b, performing domestication on juvenile fishes of the marine fishes in the low salinity salt water in a gradient mode, namely breeding the juvenile fishes which are bred in the seawater in the brackish water temporarily, formulating a domestication strategy of slowing salinity gradually, and performing domesticationon the juvenile fishes of the marine fishes through three gradients, namely the brackish water phase, the low salinity phase and the ultra-low salinity phase; c, adding a physiological salt preparation, namely adding metal ions which play a key role in osmoregulation into the water or a feed; and d, managing the breeding, namely providing a preference temperature, illumination intensity and a daily illumination period. The water circulation system and the method are mainly used for breeding the marine fishes inland.

A method for cultivating golden mushroom mycelia abundant in Se and Zn elements through liquid fermentation belongs to the field of biotechnology. The invention substantially comprises cultivation of strains, cultivation of liquid seed, namely the domestication of two elements, and enrichment, fermentation and cultivation of two elements in a deep liquid, and collecting, drying and smashing of mycelia. The dried golden mushroom mycelia abundant in organic Se and organic Zn obtained with the method of the invention has over 35Mug / g organic Se and 4.25mg / g organic Zn. The powder of the mycelia with suitable food can be used as food additives and made into functional food, which has great health care function for the people in sub-health state resulted from deficient intake of microelements of Se and Zn. The invention is high in technological content, high content in effective components of the products, high additive value, which is suitable for industrial production.

Disclosed is a method and equipment for treatment of polluted fluid, wherein ferrum family flocculating agents of a finite concentration are charged into the membrane bioreactor, in which they are subject to domestication and become compact biological ferrum sludge with improved property.

The invention relates to a kind of microbic reagent curing breed aquatics water by multi-bacteria. It can degrade and transform organic contamination of water, prohibite the growth of harmful algae and pathogeny bacteria, and promote propagation of beneficial creature, forming good entironment for growth of aquatic animal. Environment select breed and abduction select breed are adopted in this invention, spontaneous aberration and directional breed are combined, fermentated technology of original compound breed and domestication are used to make many bacteria of improved water mixed and matched each other. What' s more, growth factor and special carrier are added for promoting the growth of microbe, guaranteeing the efficiency of improvement. This product can take the place of antibiotic in feed, producing organic farm products which are green, safe and antibiotic-free.

The invention relates to a method for preparing an enzyme and bacterium composite agent for treating sewage and sludge, which comprises the following steps: (1) preparing bacillus subtilis powder; (2) preparing bacterial solution of photosynthetic bacteria; (3) obtaining indigenous bacteria by collection, culture and enrichment culture; (4) performing domestication culture of working bacteria, namely adding indigenous bacteria and a composite culture medium according to different sludge loads, adding bacillus subtilis enzyme powder in an amount which is 0.1 to 0.4 percent based on total weight and bacterial solution of photosynthetic bacteria in an amount which is 1 to 3 percent based on the total weight, performing tests under different loads and different organic matter concentration, determining an optimal parameter, and repeating for 3 times; and (5) performing domestication and amplification culture of the working bacterium composite agent, and obtaining the domesticated working bacterium composite agent in which the viable count per milliliter is cfu 10<7> to 10<9>. The enzyme and bacterium composite agent prepared by the method can treat sewage and sludge efficiency, simplyand conveniently, has very high adaptability to any sewage environment, and has a guiding and coordinating effect on beneficial decomposing bacteria in local environments.

A high efficient processing technique for hypersaline waste water generates aerobic granular sludge in a sequencing batch reactor and adopts certain startup method and sluge domestication method, in condition of without halophilic bacteria, sludge in the reactor realize aggregating growth and form composite microecology, so hypersaline waste water can be processed effectively and resistance to salinity shock of the system can be improved.

The invention discloses a repairing method of microorganism in contaminated soil of chromium slag storage yard, comprising the steps of selecting Pannonibacter phragmitetus BB single bacterium, dropping the single bacterium in a liquid culture medium containing Cr (VI) of 50-250mg / L for culturing until Cr (VI) is not detected in the culture solution, selecting strains in a bottle for transferring, gradually improving the Cr (VI) concentration for gradient domestication, adding the domesticated Pannonibacter phragmitetus BB lysate in the contaminated soil, and culturing for 4-5 days at a temperature below 30 DEG C, so as to repair the soil, wherein the amount of the added lysate is that the mass-volume ratio (g: mL) of the soil to the lysate is 1: 2. The invention treats and repairs hexavalent chromium-contaminated soil through the strains with hexavalent chromium reducing capacity, the operation is simple and feasible, the cost is low, and the method is an environment-friendly treatment method.

The invention discloses an operating method for a high-efficient anaerobic ammonium oxidation reactor. The method comprises the steps of (1) inoculating anaerobic granular sludge in an anaerobic reactor, the wherein the anaerobic granular sludge accounts for 30-90% of total volume of the reactor; and (2) taking nitrogenous simulating wastewater as reaction inflowing water, wherein the temperatureof the reactor is controlled to be in 18-37 DEG C; and in a reactor starting stage, remaining hydraulic power for 12-48h, adjusting reaction operating conditions according to concentration of ammonianitrogen and / or nitrite nitrogen, operating the anaerobic reactor for 30-150d, and forming high-efficient anaerobic ammonium oxidation granular sludge enriched with anaerobic ammonium oxidation bacteria. The method improves total nitrogen load of the reactor in a way that the load is increased gradually by lower load so as to be beneficial to stable increasing of domestication enrichment and nitrogen removing rate of the anaerobic ammonium oxidation bacteria, can effectively avoid matrix restraining of anaerobic ammonium oxidation and is beneficial to reducing process operating cost when the reactor operates under a room temperature condition.

The invention discloses a method for treating ammonia containing wastewater through simultaneous nitrification and denitrification. The method comprises the following steps: firstly, cultivating denitrifying granular sludge; and secondly, using the denitrifying granular sludge to treat ammonia containing wastewater through simultaneous nitrification and denitrification. The cultivating method of the denitrifying granular sludge comprises the following steps: firstly, inoculating seed sludge in an aerobic reactor, and performing accumulation culture to the aerobic sludge with high ammonia-nitrogen and low chemical oxygen demand (COD) wastewater; secondly, separating and screening aerobic denitrifying bacteria to perform domestication and cultivation; and thirdly, inoculating the domesticated and cultivated aerobic denitrifying bacteria in the denitrifying granular sludge to cultivate and obtain the finished denitrifying granular sludge. The invention adopts the domesticated and cultivated simultaneous nitrification and denitrification sludge treatment catalyst to treat ammonia containing wastewater, and has the characteristics of high removal rate of contaminants, low energy consumption and material consumption and the like; and nitrification and denitrification can be completed in the same reactor, and the short-range simultaneous nitrification and denitrification technology can be applied in actual wastewater treatment.

The invention relates to a method for degrading the oily sludge of a well site by using microorganisms. The method comprises the following steps of: obtaining four degrading bacteria namely pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis and acinetobacter johnsonii from the oily sludge of the well site in the loess tableland region through culturing, separation, screening, domestication and mutation, and mixing the four degrading bacteria according to the weight ratio of 1:1:1:1 to obtain a microorganism flora for degrading the oily sludge, wherein the initial pH value equals to6, the weight ratio of carbon to nitrogen equals to 100:3, and the weight ratio of carbon to phosphorus equals to 100:0.6, the mass concentration of oils in a treatment system added with mixed bacteria is reduced from 17214 mg / kg to 1257 mg / kg within 72 h in a laboratory, and the degradation rate is 92.7%. After the oily sludge with 10.55% initial oil content is experimentally treated for 56 daysin the well site, the removal rate of oil matters can reach 89.1%, and the treatment effect is obvious.

The invention relates to the preparation and application method of a medium, which is specifically related to a medium which is used for Vero cell and a culture method of the Vero cell. The invention belongs to the biological product area. The culture method of the invention is that the adhering Vero cells are suspend and cultured in SFM serum-free medium for the domestication and adaptation; the growing pattern of the Vero cell changes from adherence growth to suspension growth; the Vero cells are further amplified in Vero amplification CHEMICALLY DEFINED MEDIUM. The method of the invention has good effects; the growing pattern of the Vero cell is transformed from adherence growth to suspension growth, which changes the growth pattern of the cell and greatly increases the yield of the cell.

The invention discloses a rhizoma bletillae industrialized seedling method. According to the method, the advantage of large quantity of rhizoma bletillae capsule seeds is fully utilized; with the seeds as an explant, a rhizoma bletillae tissue culture rapid breeding technology and a tissue culture seedling domestication technology are established; germination and growth are carried out under an aseptic condition, so that the influence on external environment and germination of rhizoma bletillae seeds is overcome, and the germination rate and the growth speed of the rhizoma bletillae seeds are greatly increased. By only needing 5 months, the seeds can grow into plants of which the corm diameter is about 0.7cm, and only one-time grafting is needed in the process, so that the industrialized production of the rhizoma bletillae seeds is realized; moreover, the method is easy to operate, low in cost and high in reliability; the whole seedling period is about 8 months, and the domestication survival rate is 91 percent or above; the rhizoma bletillae seedlings which are domesticated for about 3 months are directly transplanted into a field so that the survival rate is up to about 98 percent and the rhizoma bletillae can be harvested after 4-5 years. The problem that the rhizoma bletillae seedlings are difficult to breed is solved, and the industrialized seedling of the rhizoma bletillae is realized.

The invention relates to a preparation method and a product of an H9N2 subtype avian influenza inactivated vaccine. The technical points of the invention mainly relate to the screening, the determination and the domestication of a virus-adapted cell line, the primary amplification cultivation and the continuous cultivation of a virus-adapted cell, the preparation of virus fluid by virus-inoculated culture and the preparation of final inactivated vaccine products. Firstly, the invention avoids the virus propagating method using a large amount of chick embryos in the avian influenza production at present, thereby avoiding the problem of biological potential safety hazards, and overcoming the problem that the mass production of vaccines is enslaved to the supply of the chick embryos; secondly, the invention provides a safe, continuous and closed cell culture virus production method, is used for the preparation of the H9N2 subtype avian influenza inactivated vaccine, enables the use of the cell culture method, and can simultaneously produce high-titer viruses to meet the requirements for the immunological production; and finally, the vaccine production method of the invention is simple and fast, thereby realizing the fast vaccine supply at the epidemic situation.

The invention sets up a novel technique for treating sewage of an aeration biofilter having the function of reinforced deodorization. The technique combines sewage treatment and deodorization treatment, under the prerequisite of up-to-standard discharge, and adopts a composite filler to replace the ceramsite filler of the aeration biofilter; after domestication, the aeration biofilter can have thefunction of bio-deodorization while treating sewage. The technique is implemented by filling the composite filler which is composed of ceramsite, coarse granule active carbon and iron scrap with thevolume ratio being 1:0.2:0.15 into the aeration biofilter; adopting reek to be treated to replace air to be as the aeration gas source of the aeration biofilter; and after domestication, generating high efficient deodorizing bacteria groups such as desulfurated and deaminized bacteria and the like, so as to realize treating sewage and deodorizing stink gas simultaneously in the aeration biofilter.The invention does not change the equipment parameters of the original sewage treatment system and is favorable for upgrading and modifying civil sewage and industrial sewage plants.

The invention relates to a snakehead seedling expandable compound feed and a method for comprehensively feeding snakehead seedlings by using the same. The snakehead seedling expandable compound feed has buoyancy rate of 100 percent, floats in water when fed to the snakehead seedlings, is more soft and elastic after entering the water, is closer to the meat in the aspect of physical character, hasgood body color and mouth feel as well as special taste and better water stability, and solves the difficulty of food-transferring domestication. Multiple vitamins and multiple mineral elements premix prepared in the feed is set according to the demands of the snakehead seedlings, has the functions of promoting the growth and improving the survival rate, is suitable for the nutrition demands of the snakehead seedlings, has low feed coefficient, ensures rapid snakehead growth, expands the matched feed, avoids the loss of nutrients and the pollution to water, improves the feed utilization and reduces the generation rate of diseases, thereby lowering the feeding cost, and improving the feeding benefit. The snakehead can be fed together with the chub and the bighead to gain higher economic benefit and is suitable for the northeast area of China. The meat of commercial snakehead has high quality and best mouth feel.

The invention discloses a mandarin fish feed which comprises the following components in parts by weight: 35-40 parts of fresh fish meat, 35-50 parts of white fish meal, 5-10 parts of shrimp meal, 2-5 parts of yeast powder, 2-5 parts of corn protein powder, 5-8 parts of oleum morrhuae, 1-3 parts of composite vitamin, 1-3 parts of composite mineral and 4-6 parts of adhesive. The invention also discloses an artificial feeding domestication method for the mandarin fish. The method comprises a step of feeding the mandarin fish feed. The mandarin fish feed can reduce the breeding cost and the morbidity of the mandarin fish, improve the disease resistance of the mandarin fish and realize the sustainable development of the mandarin fish breeding industry.

The invention relates to a household wine brewing machine and a method for brewing wine by using the household wine brewing machine. The household wine brewing machine can be used for overcoming the defects that the existing industrial fermentation equipment is oversized and cannot achieve miniaturization and domestication. The household wine brewing machine is divided into four parts, namely a sealing cover, a fermentation tank, a control part and a power part, wherein the fermentation tank adopts a brand-new design. The household wine brewing machine not only can be used for producing grape wine, but also can be used for producing various fruit wines and beers, so that the integrated production of various wines is realized to provide more choices for consumers, the equipment cost is lowered and the product quality is guaranteed.

The invention discloses a method for culturing chlorella. The invention provides a method for realizing mixed cultivation by taking a microorganism residue treatment solution as a main heterotrophic carbon source, which is characterized in that the mixed cultivation is carried out by conducting domestication cultivation on chlorella autotrophica under a heterotrophic condition through coupling the two processes of autrophism and heterotrophism; and after the mixed cultivation is ended and flocculated separation on a chlorella solution is ended, the chlorella solution is extracted to obtain grease, and remaining chlorella cells and microorganism residues which are not utilized can be used for further microalgae cultivation through a pretreatment process provided by the invention. In the method for culturing the chlorella, the coupling is realized on the two processes of the autrophism and the heterotrophism by using a cheap carbon source as an organic carbon source. A new way is provided for the technology for growing the grease through culturing microalgae on a large scale by the technical route of the invention.

A biological cathode domestication method for a biological cathode-type hydrogen-producing microbial electrolytic cell is provided by the invention, and is characterized by comprising: A) culturing an anode electrogenic microbial population under a microbial fuel cell mode; B) domesticating an anode hydrogen-consuming microbial population under a microbial electrolytic cell hydrogen-producing mode; C) carrying out domestication culturing of a cathode hydrogen-producing microbe under a three-electrode mode. On the basis of a further aspect of the invention, a biological cathode-type microbial electrolytic cell (400) is formed by combination of the biological anode obtained by the step A) and the biological cathode obtained by domestication culturing in the step C). The invention also provides the biological cathode-type hydrogen-producing microbial electrolytic cell, and the microbial electrolytic cell comprises the biological anode obtained by the step A) and the biological cathode obtained by domestication culturing in the step C).

The invention relates to the field of aquaculture, in particular to a comprehensive and ecological river crab pond culturing method. The method solves the problems that in an existing river crab and Chinese high fin banded shark mixed culturing method, antibiotics are usually used for carrying out prevention and treatment of diseases so as to cause reduction of nutritional value of river crabs, and the mixed culturing method brings lower economic benefits. The method comprises the following steps of step 1 of carrying out preparation of river crab pond conditions, step 2 of carrying out river crab pond preparation, step 3 of carrying out domestication of Chinese high fin banded shark, step 4 of stocking river crab larvae, fingerlings of Chinese high fin banded shark, fingerlings of mandarin fish and freshwater shrimps, and step 5 of uniformly putting fresh and clean margarya into a whole river crab pond according to the use quantity of 150kg to 200kg per mu before the Tomb-Sweeping Day and monthly putting temporarily cultured fresh margarya into the river crab pond according to the use quantity of 60kg per mu during May to August. The comprehensive and ecological river crab pond culturing method is used for culturing the river crabs.

The invention discloses a domestication cultivation method of bletilla tissue culture seedlings, which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following five steps: matrix preparation, hardening-seedling, bottling-out, domestication cultivation, and domestication management. The method has the beneficial effects that a method for transiting bletilla tissue culture seedlings from an experiment bottle to the nature is provided, so that the absorption function of root hairs, the guided transport function of stems and the photosynthesis function of leaves of tissue culture seedlings can be quickly improved, and therefore, the tissue culture seedlings can adapt to the natural environment quickly, the transplanting survival rate of tissue culture seedlings is improved, and by using the method, the transplanting survival rate of domestication seedlings reaches over 92%.

The invention discloses a construction technique for water plant system of scenic water in coastal saline-alkali area. The method aims at the characteristics such as high salt content in the soil and the water in scenic riverways in coastal saline-alkali area and utilizes highly saline reuse water washing salinity technique and salt-proof techniques such as raw soil mixed with sand and salt discharge subdrain, etc. to reduce the salt content of the soil and water of the riverway. Meanwhile, in respects of increasing the ornamental performance and strengthening the purificatory function, water pioneer plants and land pioneer plants suitable for the ecological restoration of the scenic riverways in coastal saline-alkali area are selected preferably, and are subject to domestication and optimal distribution, thus the water plant system of scenic water in coastal saline-alkali area is successfully constructed, thereby improving the environmental scenery effectively and restoring the quality of the water gradually.

The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

The invention discloses a technology for artificial domestication and breeding of sclerodermus and applied to prevention and treatment of pine sawyer beetles. The technology for artificial domestication and breeding of sclerodermus comprises the following steps of: firstly, feeding the sclerodermus to be substituted for the host of a barley pest; secondly, carrying out the selecting and refrigerating treatment of female sclerodermus; thirdly, packing the initiated pupa barley pest and the female sclerodermus into a finger-shaped tube, and plugging the tube with an ordered pine plug; and finally, neatly stacking and placing the tubes into a weather cultivation box with a cultivation temperature and conditions as well as prompts. In a bursaphelench xylophilus epidemic area, the throwing technology of sclerodermus comprises the following steps of: firstly, setting bait woods to induce the pine sawyer beetles to lay eggs; then examining the parasitic situation of the pine sawyer beetles in a pine tree section in an appropriate weather condition, and releasing the sclerodermus according to the groove number of the sawyer beetles; and finally investigating the parasitic situation of the sclerodermus. By the adoption of sawyer pests to prevent and treat the pine sawyer beetles so as to inhibit the occurrence of a bursaphelench xylophilus disease, the environment can not be polluted, the ecological balance is maintained, the biological diversity is kept, and the technology is an advanced technology for controlling the pine sawyer beetles and the bursaphelench xylophilus disease.

The invention relates to the field of cultivation technology of insects, in particular to a cultivation method of a nicotine-resistant housefly strain. According to the cultivation method of the nicotine-resistant housefly strain, firstly, housefly eggs of the nicotine-resistant housefly strain are inoculated to a first formula material, and the housefly eggs are cultivated until the larvas become mature and pupate; then after eclosion of the fly pupas, a second formula material is utilized for feeding the imagoes, and after the imagoes lay eggs, domestication of a next generation is started; operation is repeated according to the steps, and the stable nicotine-resistant housefly strain can be obtained after 20-30 generations of continuous domestication cultivation.

The invention relates to an artificial domesticating method for eleutheronema tetradactylum. The method comprises the following steps of (1) catching; (2) transportation; (3) transfer; (4) domestication; (5) feeding; and (6) sieving. Through the domestication according to the steps, young eleutheronema tetradactylum gradually grows to the specification of adult eleutheronema tetradactylum. The method has the advantages that the domestication survival rate is high, the popularization is easy, the operation is simple and convenient, the implementation is easy, the injury to the eleutheronema tetradactylum is small, and good application prospects are realized.