131 results about "Free medium" patented technology

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and / or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime / vaccinia virus boost innoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant and / or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

A medium access control method of a CSMA / CA (Carrier Sense Multiple Access with Collision Avoidance) based wireless LAN (Local Area Network) provides contention-free medium access authority to a station or access point receiving a request signal by: transmitting a request signal frame from an arbitrary station to another arbitrary station or to an access point via a medium occupied in transmission contention with a CSMA / CA algorithm using a DCF (Distributed Coordination Function) interframe space; and transmitting an acknowledgment signal frame from the station or the access point receiving the request signal frame to the station transmitting the request signal frame via an occupied medium using a short interframe space.

Media and processes for the fermentation of Clostridium botulinum and obtaining a botulinum toxin for use in formulating botulinum toxin pharmaceutical compositions. The growth media can contain significantly reduced levels of meat or dairy by-products using non-animal based products to replace the animal-derived products. Preferably, the media used are substantially free of animal derived products.

Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.

In contention and contention-free medium access methods, a first set of stations is used for no-contention and a second set of stations is used for contention. The method comprises (a) preparing a contention-free access message attempting to access a first group; (b) detecting a transmission time of a contention-free access message attempting to access a second group; (c) embedding the contention-free access message into contention access message to transmit the embedded message to at least one of the first and second groups; and (d) receiving at least one of the first and second groups a contention-free access message embedded Access messages are contended for and priority is given to either of the first and second groups to access the medium in turn. Therefore, the multi-poll distribution coordination function (MP-DCF) mechanism based on the control of polling times is shared with the commercial sites without MP-DCF modules, in order to solve the shortcomings that all sites need to have MP-DCF modules, and despite the site's The number is gradually increased to be able to maintain the throughput stably.

The invention discloses a method for promoting the accumulation of oil in microalgae and keeping the high biomass of the microalgae. The method is concretely characterized in that enrichment-cultured microalgae are cultured in a nitrogen-deficient medium containing a plant hormone. The plant hormone indoleacetic acid is added to the nitrogen-deficient medium for the first time in order to stimulate the accumulation of oil in microalgae and keep the high biomass of the microalgae, so the microalgae can still keep a high biomass under a nitrogen deficiency condition. Test proves that the method makes the oil content of chlorella higher than the oil content of the chlorella cultured in a nitrogen-free medium free from the plant hormone, so the plant hormone can be used as a good means for stimulating the microalgae to produce oil. The method has the advantages of simplicity in operation, short time, high efficiency, easiness in realization, effective increase of the biomass concentration of the microalgae under a nitrogen-deficient culture condition, and good applicability, and provides an effective technical scheme for microalgae to produce biodiesel or develop other biomass energy sources.

The invention discloses a warm-hot forming method for boron-free medium-manganese steel. The method is characterized by comprising the following steps: austenitizing pretreatment, namely heating a sheet to 750-820 DEG C at the heating rate of not lower than 10 DEG C / s, and carrying out heat preservation for 3-10 minutes; a sheet transferring process, namely cooling the sheet to a stamping temperature of 450-700 DEG C at the cooling rate of not lower than 5 DEG C / s, and transferring to a stamping mold; heat-resistant stamping and pressurizing-quenching treatment, namely transporting the sheet below the stamping mold, forming, and simultaneously carrying out heat-retaining quenching, wherein the quenching cooling rate is greater than 5 DEG / s; and the pressurizing time is 5-20s; and post-treatment, namely carrying out shot blasting on a formed sample piece, and anti-rust oil coating treatment. The warm-hot forming method provided by the invention conforms to industrial production of the boron-free medium-manganese steel, and has the advantages that the manufacture cost is reduced and the strength and plasticity of a steel part are improved; the new generation of automobile is promoted to reach a relatively high step in the aspects of light weight, fuel conservation and safety; and the warm-hot forming method is simple in technological process and convenient to operate.

The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.

The present invention provides methods for adapting cells, such as A549 cells, to growth in serum-free and animal material-free medium suspension culture. The present invention provides methods for preparing viruses, such as adenovirus, from the A549 cells adapted for growth in serum-free and animal material-free medium in suspension culture.

An evaporative cooler includes an arrangement of a combination of fluid components including flow control valves, spray bars, spray bar orifices, spray bar distribution channels, and distribution caps that produce a water application profile on the media that is adjusted to match the heat load introduced to the media by the air to be evaporatively cooled. The water evaporation rate is a direct function of this heat load profile. Applying water in this profile takes advantage of the wicking rate and flow through time constant of evaporative cooling media to effectively distribute the water through the media. This results in a once through system that allows the volume of water being applied be the lowered such that water not evaporated and exiting the media is limited and does so at very high cycles of concentration while maintaining high levels of cooling effectiveness and scale free media.

The invention provides a method for preparing medium slurry. The method comprises the following steps of: fully mixing lead-free glass powder and a colorant in certain ratio; and then mixing the mixture and an inorganic carrier to prepare the lead-free medium slurry. The invention also provides a lead-free medium slurry which consists of the lead-free glass powder, the colorant and the organic carrier; and the reflectivity of the obtained lead-free slurry can reach over 65 percent (550nm).

The invention relates to a silver base cadmium-free medium temperature solder and a preparation method thereof, which overcomes the defects of high cost of cadmium-free silver base solder, poor technical indicators of melting temperature and range, high brittleness, and narrow application range due to difficulty in machining into soldering wire. The silver base cadmium-free medium temperature solder comprises silver, copper cathodes, zinc, tin, copper-phosphorus alloy, manganese, zirconium, copper foil and lanthanum; or nickel, silver, copper cathodes, zinc, tin, copper-phosphorus alloy, manganese, zirconium, copper foil and lanthanum. The preparation method of the silver base cadmium-free medium temperature solder comprises the following steps: feeding, warming up, cooling and discharging, casting, and extruding to obtain the silver base cadmium-free medium temperature solder. The solder has the advantages of no cadmium, low content of silver, and good welding performance.

The invention provides a separation and purification method of a human umbilical cord mesenchymal stem cell exosome and an application of the human umbilical cord mesenchymal stem cell exosome, and belongs to the technical field of medicines. The human umbilical cord mesenchymal stem cell exosome provided by the invention is prepared by cultivating human umbilical cord mesenchymal stem cells via aserum-free medium, collecting supernatant liquid, implementing centrifuging as well as ultra-filtration and concentration, transferring a concentrated solution onto a 30% sucrose-heavy water densitypad and implementing further purification through sucrose density centrifuging, so that the human umbilical cord mesenchymal stem cell exosome is obtained. According to the separation and purificationmethod that the human umbilical cord mesenchymal stem cell exosome is obtained through separation and purification, immunoreactivity is effectively reduced, and a controllable dosage when the human umbilical cord mesenchymal stem cell exosome is used is guaranteed. The human umbilical cord mesenchymal stem cell exosome, by improving a degree of activating an insulin signaling pathway of a type IIdiabetes animal model, can inhibit composition and decomposition of hepatic glycogen, so that glucose metabolism homeostasis can be achieved; and meanwhile, the sensitivity of the type II diabetes animal model to insulin and an insulin secretion function of pancreatic [beta] cells can be improved and a blood glucose concentration can be reduced, so that the application of the human umbilical cordmesenchymal stem cell exosome to the preparation of medicines for treating type II diabetes can be achieved.

An object of the present invention is to provide a method for microbiologically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product after the end of culture in a biotin-free medium.

The invention relates to an aqueous alkali metal ion battery based on a colloid or gel electrolyte and a preparation method thereof. The aqueous alkali metal ion battery comprises the colloid or gel electrolyte, a positive electrode, a negative electrode, a diaphragm located between the positive electrode and the negative electrode, a positive current collector and a negative current collector respectively connected with the positive and negative electrodes, and a fluid-free medium. According to the invention, an aqueous electrolyte is replaced with the colloid or gel electrolyte, so the battery is easier to produce on the basis of the prior art and conventional processes, and has lower cost.

A hybridoma cell line producing high levels of human sequence antibody. Also described is a hybridoma cell line producing human sequence antibodies that is adapted for growth in serum-free media or animal-derived-components-free media. A hybridoma cell line producing anti-CTLA4 antibodies is most preferred.

The invention discloses a CaF2-free medium flat-field apochromatic metallographic microobjective, consisting of special optical glass convex lenses without a CaF2 material and concave lenses, wherein the number of the convex lenses and concave lenses is six in total; and all the lenses are coaxial with the center of an object surface, and the sequence of all the lenses and the object surface from right to left is as follows: the object surface, the first convex lens, the second concave lens, the third convex lens, the fourth convex lens, the fifth concave lens and the sixth convex lens. The imaging is at infinity. In the invention, the long working distance W.D is more than 10mm, the amplification factor beta is equal to 8*-10*, the focus distance f' is equal to 20-32mm, the numerical aperture (NA) is more than 0.2, and the aperture angle omega is more than 2 degrees. The CaF2-free medium flat-field apochromatic metallographic microobjective has the following advantages: 1. the flat-field apochromatic effect is achieved; 2. the working distance is long; 3. the cost performance is high; and 4. the structure is simple.

Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line. The unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. The growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production of the PICM-19H cells were evaluated for their application in artificial liver devices. PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles and display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. The data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing.

Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line and assessed for their potential application in artificial liver devices. The study included assessments of growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production. The PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles. PICM-19H cells display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. Ultrastructural analysis of the PICM-19B monolayers show that the roughly cuboidal cells display basal-apical polarization and are joined by tight junction-like complexes. Other ultrastructure features are similar to those of PICM-19H cells except that they possess numerous cell bodies resembling mucus vacuoles. The PICM-19B cells possess relatively high levels of GGT activity, but retain some inducible CYP450 activity, and some ammonia clearance and urea synthesis ability. These data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. In vitro models of the liver are needed to replace animal models for the rapid assessment of drug biotransformation and toxicity. A unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. These cells have many activities associated with xenobiotic phase I and phase II metabolism lacking in other liver cell lines. The PICM-19H cell line was also compared to the tumor-derived human HepG2 C3A cell line and to primary cultures of adult porcine hepatocytes. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing and further support their use in artificial liver device technology.

The invention provides a high-medium single-layer miniature ceramic capacitor substrate material. Raw materials comprise main ingredients and doping agents. Chemical components of the main ingredients are SrTiO3. The doping agents are composed of, by mass, mwt% of Nb2O5, nwt% of SiO2, pwt% of Y2O3, qwt% of CaSnO3 and rwt% of composite oxide additives. The preparation method includes batching, ball-milling, drying, pelleting, forming, glue discharging, reduction atmosphere sintering and oxidation heat treatment. No Pb, Cd or other volatile or heavy metal elements are contained in the formula of the high-medium single-layer miniature ceramic capacitor substrate material, and the high-medium single-layer miniature ceramic capacitor substrate material is an environment-friendly and pollution-free medium ceramic material. The sintering temperature of ceramic is 1340-1400 DEG C, the dielectric constant is high, and a certain energy-saving advantage is achieved; the raw materials are sufficiently supplied at home, price is low, and the high-medium single-layer miniature ceramic capacitor substrate material is suitable for manufacturing high-performance communication components in the modern communication technology.

The invention discloses a serum-free medium used for culture of elderly people cartilage cells, the-free medium comprises a basal medium and an additive comprising the components of the following concentration: 233muM-287 muM of vitamin C, 3.7mu M-8.9 muM of linoleic acid, 9muM-17 muM of cholesterol, 6nM-15 nM of dexamethasone, 43 muM-58mu M of acetyl cysteine, 18 mug / mL-32 mu g / mL of transferrin, 22nM-52 nM of sodium selenite, 13 muM-24 muM of sodium pantothenate, 28 muM-43 muM of biotin, 7 mug / mL-18 mu g / mL of insulin, 2 ng / mL-9 ng / mL of epidermal growth factor, 2 ng / mL-9 ng / mL of fiber growth factor, 2 ng / mL-9 ng / mL of platelet derived growth factor, and 0.5%-2.5% of human serum albumin. The cell culture time is short, and the cell biological performance is good.

The present disclosure relates to an Akkermansia muciniphila strain having a prophylactic or therapeutic effect on a degenerative brain disease and uses thereof. Since the Akkermansia muciniphila strain, the intestinal microorganism of the present disclosure, shows an effect of improving movement control and cognitive abilities as well as memory in an animal model having a degenerative brain disease such as Parkinson's disease and Alzheimer's disease, it can be useful in the prevention or treatment of brain diseases including Alzheimer's disease, Parkinson's disease, mild cognitive impairment, etc. In addition, it was confirmed in the present disclosure that compared to the Akkermansia muciniphila strain cultured in a mucin-containing medium, that cultured in a mucin-free medium showed a remarkable effect of improving hyperlipidemia, fatty liver, obesity, and hyperglycemia induced in a mouse model by high-fat diet when administered orally (in vivo). Accordingly, the present disclosure is expected to be very useful in relevant industries as a particular composition of culture medium and an optimized obligatory anaerobic culture method have been developed through the present disclosure.

The invention provides silver-based cadmium-free medium-temperature solder and a preparation method thereof and relates to medium-temperature solder and a preparation method thereof. The invention aims to solve the problems of high cost and high melting temperature of silver-based cadmium-free solder produced in the prior art. The silver-based cadmium-free medium-temperature solder is prepared from the following components in part by weight: 49 to 52 parts of silver, 14 to 16 parts of electrolytic copper, 2 to 3 parts of indium, 8 to 12 parts of stannum, 8 to 13 parts of zinc, 1.0 to 2.0 parts of copper phosphorus alloy, 1.0 to 1.5 parts of manganese, 0.2 to 0.7 part of zirconium and 5.05 to 10.8 parts of copper foil wrapped rare earth lanthanum. The preparation method particularly comprises the following steps of: 1, melting and mixing; 2, casting; and 3, extruding. The silver-based cadmium-free medium-temperature solder and the preparation method thereof have the advantages that: 1,the preparation cost is low and the prepared silver-based cadmium-free medium-temperature solder is low in temperature; 2, cadmium is not contained, so damage to a human body and environmental pollution are reduced; and 3, the plastic toughness is high, so a welding wire and a welding ring with the diameter of 0.5 to 2.0 can be prepared. The method is mainly used for preparing the silver-based cadmium-free medium-temperature solder.

A method of autologous rescue of an individual exposed to a harmful hematopoietic stem cell condition is described. The method includes removing hematopoietic stem cells from the individual, culturing the hematopoietic stem cells and reintroducing cultured hematopoietic stem cells into the individual. Stem cells are grown in a stromal-cell free medium. The harmful condition can be radiation. The culture medium can contain any of several growth stimulating factors.

The invention relates to a method of preparing single-capric lauric glyceride through step-by-step catalysis of camphor tree seed oil with acid and base, which comprises the following steps: degumming and dehydrating crude oil made from camphor tree seeds, adding the degummed and dehydrated oil into an esterification and alcoholysis reactor, adding an acid catalyst, and centrifugalizing when the acid value is reduced to be below 0.8 in reaction under the reaction conditions that the temperature is 130-140 DEG C, the amount of the consumed catalyst is 2-4 percent of the oil weight, the molar ratio of methanol to oil is 2:1 to 3:1; adding the reactants into an alcoholysis reactor, adding an alkali catalyst, and centrifugalizing when the total content of fatty acid monoglyceride reaches above 80.0 percent under the reaction conditions that the reaction temperature is 165-175 DEG C, the amount consumed catalyst is -.5-0.7 percent of the oil weight and the molar ratio of methanol to oil is 4:1 to 5:1; and spraying cold water at a temperature of 15-35 DEG C, mixing, washing, centrifugalizing, repeating the steps twice and finally dehydrating under a vacuum condition. The invention can effectively utilize the free medium-carbon-chain fatty acid in raw materials, and achieve high reaction efficiency. The fatty acid monoglyceride in the products can reach above 80.0 percent, and the content of the single-capric lauric glyceride can reach above 75.0 percent. Moreover, the invention has the advantages of reasonable process, energy conservation and environmental protection.