1024 results about "Biotransformation" patented technology

The invention provides methods for detecting variations in polymorphic sites and / or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.

The present invention provides methods for producing derivatives from cultured cells. In addition, the present invention provides methods for conversion of prenyl deerivatives, obtained from biological or petrochemical sources, to isoprene by employing chemical or biological catalysts. The present invention also provides compositions that include the cultured cells or isoprene or prenyl derivatives produced there from.

This is a method and apparatus for treatment of liquid media making use of at least one float positioned at the top of the liquid and at least one gas diffuser placed under the float and connected to this float by at least one brace, the diffuser is connected to a gas source by at least one flexible conduit. The gas emitted from the diffuser produces a mixture with liquid having density lower than the liquid and the float partially sinks in the liquid thus increasing the submergence of the diffuser and lowering the gas flow through the diffuser. At increased submergence, the gas flow is reduced, the mixture density increases, and the float rises. A repeatable motion up and down of the float-diffuser is established producing pulsations in the liquid. The method and apparatus can be used in a multitude of chemical, pharmaceutical, petrochemical, environmental and other industries for carrying out mass transfer, chemical and biological transformations, phase separations, thickening of suspensions, mixing, suspending of particles, washing, coagulation-flocculation, membrane filtration, filtration across particulate media, filtration across floating media, mass transfer across membrane, and other processes.

The invention relates to a cordyceps militaris peanut which is prepared by soaking a peanut into water, sterilizing the soaked peanut, cooling the peanut, placing the peanut into cordyceps militaris seed solution for fermental cultivation, and conducting drying after fermentation to obtain the cordyceps militaris peanut. The seed coat of the peanut has proper inbibitional effects on the cordyceps militaris when the cordyceps militaris is in biological transformation in the peanut. Therefore, by peeling the soaked peanut, the content of the fermented cordyceps militaris can be improved. Further, even inoculation area is enlarged, and biological transformation amount of cordyceps taishanensis of the cordyceps militaris can be improved. Through cellulose treatment, the peeling rate of the soaked peanut is improved, and the cordyceps militaris transformation amount of the cordyceps militaris in the peanut is improved.

A process for the treatment of liquid waste biomass, especially liquid manure compositions, wherein the biomass is converted to a fertilizer product. The process at least includes a nitrification step including a first biological conversion stage wherein ammonium is converted to nitrite using nitritifying bacteria in an aerated reactor, and a subsequent chemical oxidation stage wherein nitrite is converted to nitrate by heating the liquid waste biomass in an aerated reactor under acidic conditions. The process is particularly suitable for treating liquid manure, because of the high ammonium nitrogen contents thereof, which render the process essentially self-regulatory. In addition a process for the treatment of liquid waste biomass wherein organic matters are converted to energy sources, referred to as biogas and green cokes, and wherein nitrogen is fixed in a fertilizer product in the form of ammonium nitrate, is provided, the process including the present nitrification process.

The invention relates to a 'micro ventilation lime wood antrodia camphorate cultivation method', and belongs to the field of fungus cultivation. The method is characterized by comprising: inoculating a liquid strain onto a camphorwood section with the moisture content of 50 to 70 percent; and performing micro ventilation culture to culture fruit bodies at a temperature of 25 to 28 DEG C and a humidity of 85 to 95 percent. In the method, camphorwood is treated, the liquid strain obtained by liquid fermentation and reproduction is inoculated onto the surface of camphorwood, culture is performed in a micro ventilation mode by controlling culture temperature, humidity, light and other conditions, the growth period is 11 months, the biological conversion rate is 15 percent, the polysaccharide content is equal to that of wild antrodia camphorate, the antrodia camphorate cultivated on the lime wood has the same appearance, fragrance, bitter taste, medicinal effectiveness and the like as wild antrodia camphorate.

The invention relates to a method for cultivating tricholoma lobayense by utilizing fungus dregs in the edible fungus production field, which is characterized by utilizing the leftovers, namely fungusdregs, of the edible fungi instead of cottonseed hulls to cultivate the tricholoma lobayense, utilizing the method of biological fermentation to treat the fungus dregs and adding bran and other nutrients to form culture media, and effectively realizing cultivation of the tricholoma lobayense through the process flows of bagging, sterilizing, inoculating, hypha fermenting, cultivating, bag removing, soil covering, fruiting and harvesting, etc., wherein, the leftovers of the edible fungi are used for cultivating oyster mushrooms and needle mushrooms, etc. The method not only realizes resource of the organic wastes and reduces pollution of the organic wastes on the environment by developing and utilizing the leftovers of the edible fungi and conforms to the requirement on striving to developcircular economy encouraged by the state, but also greatly reduces the usage amount of the cottonseed hulls and other substances for cultivation, effectively lowers the production cost of the tricholoma lobayense, increases the cultivation benefits and ensures the biological conversion ratio to be more than 80%. The method utilizes the wastes and attains two objectives, thus being suitable for popularization and application in the rural areas.

The present invention is a kind of tobacco perfume and its preparation process. The tobacco perfume is prepared through the following steps: 1. crushing the materials including balloon flower root 150-250 weight portions, fritillary bulb 30-80 weight portions, root of straight lady bell 150-250 weight portions, licorice 30-80 weight portions, tuckahoe 150-250 weight portions and Dangshen 70-130 weight portions, adding water and inoculating microbe strain to ferment to obtain fermented product; and 2. extracting the fermented product with water soluble solvent, concentrating the extracted liquid to obtain tobacco perfume paste. Compared with available technology, the present invention has the advantages of the microbial fermentation process, mallow flavor and obvious cigarette modifying effect of the tobacco perfume.

The invention provides a recombinant escherichia coli for high-yield tetrahydropyrimidine and a method for preparing tetrahydropyrimidine by using the recombinant escherichia coli. The recombinant escherichia coli provided by the invention is prepared by importing Halomonas elongate EctABC containing recombinant plasmids into escherichia coli. The recombinant escherichia coli disclosed by the invention realizes the soluble expression of three key enzymes synthesized by tetrahydropyrimidine under the adjustment and control of an arabinose promoter. Thalli subjected to induced expression implements the efficient secretory expression of tetrahydropyrimidine by taking sodium aspartate as a precursor through a bioconversion method. Thalli per gram can synthesize 1.1 grams of tetrahydropyrimidine, and more than 90% of tetrahydropyrimidine is secreted to extracellular receptors. The method for preparing tetrahydropyrimidine by using the recombinant escherichia coli provided by the invention facilitates the downstream purification and separation of products, and has great significance on the industrial production and large-scale application of tetrahydropyrimidine.

An additive of forage for increasing the content of peptide, the utilization rate of protein and the growth speed of animals is prepared from one or more kinds of feed protein through solid fermenting by bacillus and / or bacterium lactis and / or yeast.

The invention relates to an edible fungus compost, a production method thereof and an edible fungus culture process. The edible fungus compost disclosed by the invention consists of 40 to 90 parts of residual waste (spent mushroom substrate) left over after the harvest of edible fungi, 5 to 40 parts of cottonseed hulls, 3 to 20 parts of bran, 2 to 8 parts of corn meal, 1 to 2 parts of gypsum powder, 0 to 5 parts of quicklime, 0 to 2 parts of superphosphate and 0.05 to 0.8 parts of fungus-strengthening substance, and the pH value is 6 to 9. The invention adopts the residual waste (spent mushroom substrate) left over after the harvest of edible fungi as main material ingredient to produce the edible fungus compost. By implementing the invention, the residual waste of edible fungi cannot pollute the environment any more, and is made profitable, and the resource can be recycled; an edible fungus material resource is added, the economic burden caused by the shortage and higher prices of edible fungus materials is decreased for edible fungus farmers, the production cost of the method is reduced by more than 50 percent in comparison with the production cost of the conventional method, the yield is increased by 10 to 15 percent, the bioconversion rate reaches 80 to 120 percent, and economic income and social benefit are increased. The method is applicable to a variety of edible fungi.

The invention relates to a method for extracting, separating and purifying a functionality active component Astragaloside IV from a common used traditional Chinese medicine Astragalus mongholicus, and aims to provide a simple, safe, economic and effective method for extracting, separating and purifying the Astragaloside IV from the Astragalus mongholicus with high purity. The adopted technical scheme is as follows: taking traditional Chinese medicine Astragalus mongholicus as a raw material; and adopting a series of original and efficient technologies for extracting, separation and purifying such as homogenate extraction-mixing enzyme induction biotransformation technology, negative pressure cavitation suspension extraction technology, saponin derivant hydrolysis converting technology, liquid-liquid extraction technology, macroporous absorption resin enrichment technology, normal phase silica gel medium pressure column chromatography technology and devitrification at a low temperature, recrystallization technology and the like to obtain the Astragaloside IV with high purity and yield, wherein the yield thereof can be more than 0.08% and the purity can be more than 95%. The raw material used in the invention is the common used traditional Chinese medicine Astragalus mongholicus, and has abundant resources; the production process of the Astragaloside IV is simple and practicable; the target compound has high yield and purity; and the method is suitable for the industry production and application, has high additional value and excellent market application prospective.

The invention discloses lignocellulose degrading bacteria and application thereof. The lignocellulose degrading bacteria is expanded penicillium W4 with the preservation number of CGMCC (China General Microbiological Culture Collection) No.4077. The stain can generate cellulose and laccase and degrade straw lignocellulose, can be used as a main effective component of microbial fungicides of a mushroom culture medium and has the effects of shortening the biological transformation time of the mushroom culture medium and improving the quality of the mushroom culture medium. Particularly for the biological transformation of a small quantity of packed mushroom culture media of a family workshop, the lignocellulose degrading bacteria has obvious advantage in environment with lower temperature.

The invention relates to application of an alpha-L-rhamnoside enzyme in the directional synthesis of isoquercitrin by the biological conversion of rutin. Because the crude enzyme preparation or the immobilized enzyme preparation of the alpha-L-rhamnoside enzyme is used for catalyzing and hydrolyzing the rutin and directionally and biologically synthesizing the isoquercitrin, the application has the advantages of wide source, easy preparation and low cost of catalysts, high stability, and high catalysis efficiency and specificity of the enzyme preparation, and is easy to store. Thus, the alpha-L-rhamnoside enzyme can greatly reduce the production cost of quercetin, the catalysis and conversion rate of the enzymes is high, and the products are single.

The invention discloses a culture material for pleurotus nebrodensis and a cultivation method of the pleurotus nebrodensis. The culture material is prepared from the following components by weight percent: 50-60% of corn cob, 7-12% of lotus receptacle, 8-12% of peanut shells, 10-15% of grape seeds, 5-15% of corn flour, 1-2% of vinasse, and 1-2% of calcium carbonate. The cultivation method comprises the steps of sterilizing the culture material, inoculating after cooling, culturing hypha for 25-35 days at 25-28 DEG C, keeping the humidity of the environment to be 75-95% with 1wt% of a water-soluble carbon nano tube solution, transferring the cultured hypha into a fruiting chamber after mediotrastum dispersing, growing sporocarp for 10-12 days at 13-18 DEG C and harvesting. The culture material and the cultivation method of the pleurotus nebrodensis is characterized in that the grape seeds contain rich multi-amino acids, vitamins and mineral substances, can increase the yield, improve the quality of the pleurotus nebrodensis and increase the biotransformation efficiency of the pleurotus nebrodensis; a water-soluble carbon nano tube has the effects of promoting the plant growth, preventing from damage by external conditions and the like, and can increase the yield, improve the quality of the pleurotus nebrodensis and shorten the growth period of the pleurotus nebrodensis hypha; besides, the grown pleurotus nebrodensis has the good quality and a large pleurotus nebrodensis body.

A method of producing ectoine by high-density culture of recombinant escherichia coli is provided. The method includes subjecting sodium L-aspartate to a biotransformation reaction by utilizing escherichia coli BW-p-BAD-ectABC having an accession number of CGMCC NO.8334. According to the method, 87.5 g of extracellular ectoine can be synthesized in each liter of fermenting bacteria after the fermenting bacteria are used repeatedly for five times, the synthetic efficiency is 11.67 g / L.d, and both the synthetic yield and the synthetic efficiency are higher than the reported synthesis levels. The method has great significance for industrial production and large-scale application of the ectoine.

The invention provides a butyrylcholinesterase variant having the amino acid sequence selected from SEQ ID NOS: 4, 6, 8, 10, 12, 14, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, and 196, or functional fragment thereof. In addition, the invention provides a method of converting a camptothecin derivative to a topoisomerase inhibitor by contacting the camptothecin derivative with a butyrylcholinesterase variant selected from SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, and 196, or functional fragment thereof, under conditions that allow conversion of a camptothecin derivative to a topoisomerase inhibitor. Further, the invention provides a method of treating cancer by administering to an individual an effective amount of a butyrylcholinesterase variant selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, and 196, or functional fragment thereof, exhibiting increased capability to convert a camptothecin derivative to a topoisomerase inhibitor compared to butyrylcholinesterase.

The present invention is directed to a process for biologically converting carbohydrates from lignocellulosic biomass comprising the steps of: suspending lignocellulosic biomass in a flow-through reactor, passing a reaction solution into the reactor, wherein the solution is absorbed into the biomass substrate and at least a portion of the solution migrates through said biomass substrate to a liquid reservoir, recirculating the reaction solution in the liquid reservoir at least once to be absorbed into and migrate through the biomass substrate again. The biological converting of the may involve hydrolyzing cellulose, hemicellulose, or a combination thereof to form oligosaccharides, monomelic sugars, or a combination thereof; fermenting oligosaccharides, monomelic sugars, or a combination thereof to produce ethanol, or a combination thereof. The process can further comprise removing the reaction solution and processing the solution to separate the ethanol produced from non-fermented solids.

The invention relates to a biological preparation method of an intermediate of atazanavir. (S)-tertiary butyl (4-chloro-3-carbonyl-1-benzene butyl-2-yl) carbamic acid ester is taken as a substrate. The preparation method comprises the following steps of adding the substrate, a cosolvent and glucose into a reactor, stirring uniformly, weighing ketoreductase powder, glucose dehydrogenase and cofactor, dissolving in a water phase buffered solution with pH of 8-9, adding the obtained mixed solution into the reactor, stirring, controlling the temperature to be at 32DEG C-34 DEG C, and beginning reacting, wherein at the beginning of the reaction, the mass volume ratio concentration of the substrate is 0.05-0.2g / ml, and the mass ratio of the added ketoreductase to cofactor to glucose to the substrate is (0.02-0.03):(0.001-0.005):(0.8-1.2):1. Compared with the prior art, the biological preparation method realizes more environment-friendly and mild biological conversion process with high reaction efficiency, and has important application value.

The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.

The invention relates to saccharification processing technology of jerusalem artichoke by utilizing high-quality fructose biomass as a raw material and strain selection and technology optimization for producing mannitol by taking jerusalem artichoke as carbon source through fermentation. The method comprises the following steps: 1) crushing jerusalem artichoke tuber into coarse particles, filtering after water leaching and enzymolysis for 6 hours, supernating at 42 DEG C, rotating, evaporating and concentrating to obtain saccharification jerusalem artichoke juice with high concentration of fructose; 2) establishing high performance liquid chromatography analysis and detection conditions which can synchronously analyze the content of fermentation liquor substrate (glucose and fructose) and products (mannitol); and 3) inspecting the capacity for producing lactic acid and mannitol through fermentation by seven lactic acid bacteria by utilizing saccharification jerusalem artichoke juice with different concentration of total sugar, thus determining lactic acid bacteria with high transformation rate and production intensity of fructose, and optimizing production fermentation conditions and the highest concentration of tolerant substrate. Through feed-batch fermentation, production efficiency can be improved and mannitol can be continuously produced in large scale. The method not only generates no byproduct of sorbitol, but also has low production cost, wide raw material sources, simple technology, and mature technical route and can be implemented in industrialization.

The invention discloses a method for preparing (R)-2-hydroxy-4-phenyl ethyl butyrate by catalyzing recombinant carbonyl reductase, which belongs to the technical field of biological engineering. The method comprises the following steps of: cloning gene segments of carbonyl reductase (IolS) and glucose dehydrogenase (GDH) from bacillus subtilis CGMCC NO.1.1508, expressing an IolS gene and a GDH gene in series by adopting a dual-starter method to construct a recombinant plasmid pET24a-G-T7-I, and introducing the plasmid into escherichia coli BL21(DE3); and under the condition of not adding or adding a small amount of NADP+cofactors, performing biotransformation by taking a cell-free extract of the escherichia coli recombinant plasmid as a catalyst, 2-oxo-4-phenyl ethyl butyrate as a substrate and glucose as a substrate to obtain (R)-2-hydroxy-4-phenyl ethyl butyrate, wherein the enantiomeric excess value of the product is higher than 99.5 percent. In the method, IolS and GDH are co-expressed, so that efficient regeneration of an intra-cellular cofactor NADP(H) is realized, production cost is lowered, and a good industrial application prospect is achieved.

The invention provides a fibration Cellulomonas spp and a hydrolytic enzyme thereof for effectively transforming taxane xyloside into taxol or the analogues thereof, and an effective new method for preparing the taxol or the analogues thereof through biotransformation reaction. The method can prepare the taxol or the analogues thereof with high efficiency, low cost and environmental protection, which provides an effective approach adapting to industrial production for making full use of taxane resources.

The invention provides a preparation method and an application of a discarded tobacco leaf fermentation extract. Discarded tobacco leaves are fermented with beta-glucosidase, a tobacco biotransformation liquid containing various increased small-molecular aroma substances is obtained through enzymatic curing, then amino acid and reducing sugar in certain proportion are added for a high-temperature Maillard reaction under an alkaline condition, a natural tobacco Maillard reaction liquid is obtained and subjected to pumping filtration, a filtrate and filter residues are obtained, the filtrate is concentrated and mixed with propylene glycol and the like, further separation and purification are performed through molecular distillation, and light components are collected and taken as the discarded tobacco leaf fermentation extract. Reconstituted tobacco substrates with low irritation, low miscellaneous gas, low nicotine content and low harm are prepared from the filter residues with a paper-making process, the quality of the thin discarded tobacco leaf substrates is improved, the use effect of the discarded tobacco leaves on cigarette products is improved, the adding quantity of the extract is increased, the extract can be applied to an atomization filler material for novel carbon heating tobacco, and the quality of the atomization filler material is improved.

The invention provides a method for oriented biosynthesis of GAMG, and belongs to the technical fields of biosynthesis and biotransformation. The method comprises the following steps: producing corresponding beta-D-glucuronidase by penicillium purpurogenum stoll in a fermentation medium; adding the obtained beta-D-glucuronidase into conversion solution containing glycyrrhizic acid substrate, orientedly hydrolyzing the glycyrrhizic acid under the action of enzyme, and obtaining a coarse product of GAMG; and performing organic solvent extraction, resin adsorption and purification to obtain a GAMG product. The method takes microbialenzyme as a catalyst, and has high reaction efficiency, no pollution, simple steps, and low cost; and the product is single and has high purity.

The invention discloses a method for the solid state fermentation processing treatment of a Chinese traditional medicine, which is characterized in that the method comprises the steps of the screening and acclimation of Chinese medicine fermentation medical microbes, the preparation of Chinese medicine solid state fermentation culture medium and the solid state fermentation processing of the Chinese medicine. In the method, the solid state fermentation of the microbes is used as a method for processing the Chinese medicine, thereby breaking the limitation that the prior solid state fermentation Chinese medicine processing is mainly the self fermentation of fungus Chinese medicine and widening the source of the fungus species for the Chinese medicine solid state fermentation processing. Moreover, the method fully utilizes the microbe conversion and biological catalytic action, improves chemical compositions and the efficacy of the Chinese medicine compound and promoting the Maillard reaction of the Chinese medicine compound.