The process of preparing catalyst for C8 arene isomerization includes forming the mixture of EUO type molecular sieve and adhesive to obtain carrier, introducing VIII metal, drying, roasting and reducing with reductant gas. The catalyst contains EUO type molecular sieve in 5-90 wt%, adhesive 10-90 wt%, and VIII metal 0.1-5.0 wt%. The EUO type molecular sieve is prepared through mixing compound containing element X, compound containing element T, alkali metal compound and water, hydrothermal crystallization reaction in the presence of template agent precursor compounded with coarse dibromoalkane and monamine, collecting the solid and drying, where X is Si and Ge, T is Al, Fe, Ga or B, and the coarse dibromoalkane is mixture of dibromoalkane in the content of 80-95 wt%, Monobromoalkane and paraffin hydrocarbon diol. The catalyst has high ethyl benzene converting rate and low arene losing rate.