5221 results about "Nicotiana tabacum" patented technology

The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate Nicotiana tobacum plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved Nicotiana tobacum plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.

Aspects of the present invention concern tobacco having reduced nicotine and nitrosamines, which is used to manufacture consumer tobacco products, and methods to produce such tobacco. More specifically, embodiments include methods to reduce nicotine and nitrosamines in tobacco crops by applying auxin, auxin analogs, and jasmonate antagonists during the cultivation of such tobacco crops. Tobacco generated using said methods and consumer tobacco products obtained therefrom are also embodiments.

An improved vaporizer unit and water pipe assembly for extracting ingestible vapors from tobacco and herbs. Preferable embodiments of said assembly may comprise blown glass, colored glass, and adaptations for the safety of the user and to protect against breakability of said assembly. Other preferred features and embodiments include but are not limited to a cage secured to protect a central vessel housing a heating means, a hanger for storing, enhanced airflow, enhanced draw, and portability.

The application provides, in part, vectors based on novel tobacco rattle virus replicons, as well as methods for using such vectors and transgenic plants.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

This invention discloses a method for manufacturing an insecticidal organic fertilizer. The fertilizer is composed of human or animal excrement, straw powder, tree leaves, wine lees, drug residues, sawdust, edible fungi residues, furfural residues, lime powder, fermentation agent, phosphorus- and potassium-leaching bacteria, tobacco powder, Ricinus communis leaf powder, Zanthoxylum cake powder, Datura and Strychnos nuxvomica. The fertilizer has such advantages as wide raw material resources, low cost, convenient usage, abundant nutrition, low fertilizer application amount, high efficiency, no toxicity, no pollution and no residues.

The invention is a chewing tobacco substitute made from natural leaves and additives which simulate the taste and consistency of chewing tobacco to which a nicotine compound is added. The invention allows those addicted to chewing tobacco to chew and receive nicotine without incurring the other harmful side effects of tobacco. In one embodiment of the invention the product is provided with varying levels of nicotine.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

The invention provides a method of extracting sucrose esters from Oriental tobacco, the method comprising: providing a Oriental tobacco material; contacting the solid Oriental tobacco material with a supercritical fluid, such as supercritical carbon dioxide, for a time and under conditions sufficient to extract sucrose esters from the Oriental tobacco into the supercritical fluid; and separating the supercritical fluid comprising extracted sucrose esters from the Oriental tobacco. The resulting extract can comprise more than 90% by weight of tetra-acyl sucrose esters, and can be used as a flavor additive for tobacco materials used in smoking articles.

Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.