The invention discloses a special culture material formula capable of remarkably improving content of various amino acids in dictyophora fruit body and a cultivation method. The special culture material formula is characterized by comprising the following raw materials in parts by weight: 20-30 parts of bamboo, 20-30 parts of wood chips or sawdust or roots of other trees, 20-30 parts of bagasse, 10-20 parts of chaff, 1-3 parts of urea (or 5-10 parts of ammonia bicarbonate), 0.3-0.5 parts of potassium dihydrogen phosphate, 0.2-0.3 parts of magnesium sulfate and 1.0-1.2 parts of calcium carbonate, wherein 20-40kg of swine waste is added into every 100kg of mixed cultivation material. The cultivation method comprises the following steps of: selection of a cultivation place, preparation of materials, stockpile fermentation, blanking and seeding, earthing, spawn running and harvesting. According to the invention, the content of various amino acids in dictyophora fruit body is remarkably improved, the discharge of pollutants in the pig industry can be reduced, the pollutants of the pig industry are reduced, and the resource recycling of the swine waste is realized.