Method for preparing champignon polypeptide powder
A technology of polypeptide powder and shiitake mushrooms, which is applied in the field of deep processing of shiitake mushrooms, can solve the problems of poor utilization of abundant shiitake mushrooms, waste of resources, etc., and achieve the effects of low production cost, high freshness, and high amino acid content
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Embodiment 1
[0015] 1) Pulverize the fruiting bodies of shiitake mushrooms, take the fruiting body powder of shiitake mushrooms and mix with 8 times the amount of water;
[0016] 2) At 45°C, first add 1‰ cellulase (Celluse 2.0L, Novozymes) for enzymolysis for 1 hour, then add 1‰ endopeptidase (compound protease, Novozymes) for enzymolysis for 1 hour, and then Add 3‰ of peptide bond exonuclease (flavored protease, Novozymes) to hydrolyze for 6h;
[0017] 3) Heat up to 95°C, keep warm for 30 minutes to inactivate the enzyme, then concentrate the enzymolysis solution, add 4% salt, 2% monosodium glutamate, and 2% maltodextrin to the concentrated enzymolysis solution, and embed at 90°C for 1 hour , control the inlet air temperature at 180-200°C, and the outlet air temperature at 75-85°C, and spray dry to obtain the product.
Embodiment 2
[0019] 1) Crush the fruiting bodies of shiitake mushrooms, take the fruiting body powder of shiitake mushrooms and add 10 times the amount of water to mix;
[0020] 2) At 50°C, first add 1‰ cellulase (Novozymes) for enzymolysis for 2 hours, then add 2‰ endopeptide bond enzyme (papain A1, Guangxi Pangbo Biotechnology) for enzymolysis for 1 hour, and then add 4 ‰ of peptide bond exonuclease (flavored protease, Guangxi Pangbo Biotechnology) enzymolysis 4h;
[0021] 3) Heat up to 95°C, keep warm for 30 minutes to inactivate the enzyme, then concentrate the enzymolysis solution, add 4% salt, 2% monosodium glutamate, and 2% maltodextrin to the concentrated enzymolysis solution, and embed at 90°C for 1 hour , control the inlet air temperature at 180-200°C, and the outlet air temperature at 75-85°C, and spray dry to obtain the product.
Embodiment 3
[0023] 1) Pulverize the fruiting bodies of shiitake mushrooms, take the fruiting body powder of shiitake mushrooms and add 12 times the amount of water to mix;
[0024] 2) At 55°C, first add 1‰ cellulase (Celluse 2.0L, Novozymes) for enzymolysis for 1 hour, then add 1‰ endopeptidase (compound protease, Novozymes) for enzymolysis for 1 hour, and then Add 3‰ of peptide bond exonuclease (flavored protease, Guangxi Pangbo Biotechnology) to hydrolyze for 6h;
[0025] 3) Raise the temperature to 95°C, keep it warm for 30 minutes to inactivate the enzyme, then concentrate the enzymolysis solution, add 4% salt, 2% monosodium glutamate, and 4% maltodextrin to the concentrated enzymolysis solution, and embed at 90°C for 1 hour , control the inlet air temperature at 180-200°C, and the outlet air temperature at 75-85°C, and spray dry to obtain the product.
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