The invention discloses a method for measuring ammonia content in cigarette smoke through ion chromatography. The method comprises the steps that an F319-04 filter leaf and an absorption bottle gather ammonia in main stream smoke, extract liquor is added to extract particulate matter in the F319-04 filter leaf, moderate extract liquor is mixed with absorption liquid in the absorption bottle, volume metering is conducted, an aqueous phase filter is used for filtering the mixed liquid, and an ion chromatograph is introduced to conduct measuring, wherein the extract liquor is aldehyde with strong acid. Filter leaf extraction is conducted by the aldehyde solvent with the strong acid, and therefore the stability of ammonia detection liquid can be effectively improved. The method for measuring the ammonia content in the cigarette smoke through the ion chromatography can measure the ammonia content in the sample solution accurately and fast through the ion chromatography, and is suitable for measurement of the ammonia content in large numbers of cigarette samples, good in practicability and beneficial to deep harm-reduction and tar-reduction research.