5873 results about "Chromatographic column" patented technology

The invention concerns a chromatographic device comprising: a column (12) for receiving a chromatographic bed (14) through which flows a first fluid; a piston (36) sliding inside the column (12) and exerting pressure on the bed (14), a chamber (28) containing a second fluid exerting pressure on the piston (36); a regulator (52) for the pressure exerted by the piston (36) on the bed based on the pressure inside the chamber (28), on the flow pressure of the first fluid at the intake of the column (12) and on a reference pressure on the bed (14). Said device enables to separate at least two compounds to be subjected to chromatographic analysis, while adjusting the pressure exerted on the chromatographic bed. The device efficiently uses the chromatographic bed while protecting it.

A method for extracting and refining rutin comprises the following steps of (1) extraction: smashing raw materials, adding organic solvent into the smashed raw materials, performing extraction for 2-4 times, mixing extracting solution and concentrating the mixed extracting solution to thick extract with organic solvent content smaller than or equal to 5wt%; (2) alkali dissolution-acid sediment: adding alkaline water into the thick extract, then heating the mixture and stirring, filtering the heated mixture, regulating pH of alkaline filtrate to be 3-4, standing, filtering, drying to obtain a rutin pure product; and (3) mother liquor treatment: enabling obtained acid mother liquor to penetrate through a chromatographic column filled with resin, after the acid mother liquor penetrates through the chromatographic column, firstly using deionized water to wash a column bed until outflow liquid is neutral and colorless, introducing eluent into the chromatographic column and collecting eluate until outflow liquid is colorless, concentrating the eluate to the volume which is 5-10% of the original volume in a pressure reduction mode, cooling the concentrated eluate to achieve crystallization, filtering, using the deionized water to elute a filter cake, and drying the eluted filter cake to obtain a mother liquor rutin product. The prepared rutin is high in yield and purity, and the quality of the rutin reaches the international standard.

A liquid chromatographic system is provided including catalytically combining hydrogen and oxygen gases in the chromatography eluent stream in a catalytic gas elimination chamber, to form water and thereby reduce the gas content in the eluent stream. Also, a liquid ion chromatographic system in which the effluent from the detector is recycled to a membrane suppressor and then is mixed with a source of eluent for recycle to the chromatographic column.

The present invention provides a straightforward and robust synthetic process for producing a chromatographic column with eluent-sensitive light diffracting properties based on an inherent photonic band structure and a chromatographic device using the chromatographic column. The present invention provides chromatographic devices employing a chromatographic column which in one embodiment is a photonic colloidal crystal which includes an assembly of colloidal microspheres assembled into a highly ordered array within a housing such as a tube with the highly ordered array being a photonic crystal along the length of the crystal, and a second embodiment which is an inverse construct of the first embodiment, where solid microspheres making up the photonic colloidal crystal chromatographic column are replaced with spherical voids or void spaces subsequent to infiltration of a material of selected refractive index. The photonic band structures of the first type of column made with colloidal particles and the second type of column made by inverting the first type of column may include a photonic band gap, a fundamental stop-band, higher stop-bands, or combinations thereof.

A process for preparing danshinolic acid A from red sage root includes such steps as extracting in water or alcohol, concentrating, high-temp and -pressure reaction, purifying by chromatographic column, regulating pH value, extracting in organic solvent, concentrating and drying.

The process of extracting momordica glycoside from momordica grosvenori includes the following steps: extracting momordica grosvenori material with water or low carbon alcohol as solvent and in a heating, ultrasonic or microwave process; concentration; and separation and purification with chromatographic column or membrane. The extract has momordica glycoside content of 40-80 wt% or momordica glycoside V content of 20-40 wt%. The present invention has high momordica glycoside content in the product, less loss of active component, simple production process and short production period.

The invention discloses polymorphs A, B, C and D of abiraterone acetate. A preparation method of the polymorphs comprises the step of re-crystallizing the abiraterone acetate subjected to the separation and the purification of a chromatographic column in different solvents. Through stable investigation, four polymorphs have favorable stability and flowability, can be used as raw materials for storage and transportation and are suitably applied to antitumor medicinal preparations.

A liquid chromatographic system is provided including catalytically combining hydrogen and oxygen gases in the chromatography eluent stream in a catalytic gas elimination chamber, to form water and thereby reduce the gas content in the eluent stream. Also, a liquid ion chromatographic system in which the effluent from the detector is recycled to a membrane suppressor and then is mixed with a source of eluent for recycle to the chromatographic column.

Systems and methods are disclosed for producing customized, predictable and reproducible supplies of radioisotopes using, for example, a reactor housing that is fabricated from a radioactive shielding material and has both an internal volume and a surface that comprises an entry port and an exit port, a chromatographic column that is positioned within said internal volume such that a first end of said column is in fluid communication with said entry port and a second end of said column is in fluid communication with said exit port, and a changeable filter module that is disposed external to said reactor housing and in fluid communication with said exit port.

The invention discloses a preparation method of a brivaracetam intermediate shown in B-VI. The preparation method comprises the following steps of dissolving B-IV and R-phenylethylamine into a solvent, crystallizing, filtering, and recrystallizing, so as to obtain B-V; then, converting into B-VI. The preparation method has the advantages that a chiral chromatographic column separation isomer is not needed in the preparation process, and only the simple steps of extraction, washing, drying, concentration and the like are performed, so as to separate effective ingredients; the separation process is simple, and the production cost of brivaracetam is greatly reduced. A formula is shown in the description.

The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising diionic liquids and the use of diionic liquids as the stationary phase in a gas chromatographic column.

The invention belongs to the technical field of chemical industry, and particularly relates to a method for enriching cannabidiol. The method includes steps of S1, drying Cannabis sativa flower and leaf for 0.5-4 hours at 120 DEG C, and smashing until the particle size of the smashed material meets the requirement of over 50 meshes; performing carbon dioxide supercritical extraction for 1-9 hours under the conditions of 30-55 DEG C and 13-30 MPa, and recycling carbon dioxide to obtain initial extractive of Cannabis sativa flower and leaf; S2, dissolving the initial extractive of Cannabis sativa flower and leaf in ethanol or methanol, refining, filtering and removing impurities, distilling and drying the solvent for future use; S3, filling a chromatographic column with the pretreated filler, sampling by a wet method or a dry method, wherein the sampling amount is 3-15 wt%; S4, performing gradient elution or isocratic elution by one or mixture of water, methanol, ethanol, n-butyl alcohol, acetone or chloroform; collecting the dilution section with enriched cannabidiol. The method is simple in technique; content of cannabidiol is improved, and tetrahydrocannabinol content is reduced.

The invention discloses a method for determining content of volatile carbonyl compound in main stream smoke of cigarette. 2, 4-dinitrophenylhydrazine is utilized to gather volatile carbonyl compound in main stream smoke of cigarette, so as to form hydrazone compound, and the content of the hydrazone compound is determined by a high performance liquid chromatograph by adopting external standard method; and the high performance liquid chromatograph adopts chromatographic column. The determination method of the invention adopts chromatographic column, has excellent separating effect on nitro substituted arene derivative, especially on hydrazone compound formed after reaction of 2, 4-dinitrophenylhydrazine and volatile carbonyl compound, and meanwhile chromatographic analysis condition is optimized, thus achieving complete separation of volatile carbonyl compound and interfering component in main stream smoke of cigarette and improving accuracy of determination.

The present invention relates to diionic liquid salts of dicationic or dianionic molecules, as well as solvents comprising such diionic liquids and the use of such diionic liquids as the stationary phase in a gas chromatographic column.

The present invention relates to a method of separating sugars and sugar alcohols from each other. More particularly the present invention relates to the use of a weakly basic anion exchange resin in a chromatographic separation process. The advantage of the present invention compared with the prior art is that it is especially suitable for separating reducing sugars in acidic conditions as well as for example in weakly acidic conditions. The method using chromatographic separation comprises at least one step where a weakly basic anion exchange resin is used in a chromatographic column or in a part of a column.

The invention discloses a method for preparing methionine. The method for preparing the methionine concretely comprises the steps that cyanohydrins, carbon dioxide and ammonia or sodium cyanide, 3-(Methylthio)propionaldehyde, carbon dioxide and ammonia serve as raw materials to prepare hydantoin; alkaline hydrolysis is conducted on the hydantoin and then the hydantoin is acidized to pH 3.0-6.0 to obtain a compound of the methionine and salts; crystallization is conducted under the condition of temperature ranges from 8 DEG C to 45 DEG C, then the compound is separated to obtain a methionine crystal and salt mother liquor, the methionine crystal is washed by water and dried, and washing liquor is standby; finally, the salt mother liquor is concentrated under the condition of temperature which is lower than 60 DEG C, and then the salt mother liquor passes through a chromatographic column to obtain an inorganic salt and the methionine; the preparation method can efficiently separate the methionine and the salts, the recovery rate of the methionine can reach 99%, and the method is green and protects the environment, no methionine is resolved, no large number of acid and stink wastewater is discharged, and the purity of the inorganic salt is high and can be sold as a commodity.

The present invention discloses a kind of respiration detecting method and apparatus for diagnosing lung cancer in early stage. The method includes solid phase micro extraction to pre-enrich the exhaled gas of the patient, inserting the solid phase micro extraction with adsorbed organic gas component into hot gas desorbing unit to desorb, separating the desorbed organic gas component with vapor capillary chromatographic column, adsorbing the organic gas component with the organic gas sensing film in the detecting head of one pair of HF delay line ASW sensors to detect the organic gas content, and performing image processing and nerve network mode distinction on 11 kinds of detected lung cancer labeling organic components to diagnose lung cancer patient. The technology of the present invention may be also used for detecting volatile organic matters in other various fields.

In a single-ended or differential instrument amplifier, an input offset may be adjusted by driving current into the impedance of a feedback network of the amplifier. The amplifier may be provided with programmable gain capability. The impedance does not change with different gain settings, such that the input offset adjustment is equal for all gains. The amplifier may receive the output of a sensor such as, for example, a gas detector such as a thermal conductivity detector. The gas detector may be utilized to measure a gas flowing from a gas source such as, for example, a chromatographic column.

The invention belongs to the technical field of biological analysis and detection, in particular relates to a biological detection microfluidic chip based on immune reaction and a preparation method thereof. The chip takes optically transparent polydimethylsiloxane as a material and comprises a sample channel layer, a valve control layer and a substrate layer. The chip contains a sample enrichment and immunoassay module which comprises one or a plurality of nanolitre-size immune chromatographic column micro-analysis rooms, wherein each analysis room is fixed with antibody protein or antigen and is used for realizing rapid field detection of a plurality of antigen samples, such as bacteria, viruses and various toxins. The invention has the characteristics of fast speed, high efficiency, convenient carrying, low cost and easy automation control, and is capable of finishing automatic signal acquisition, remote transmission and signal analysis and suitable for rapid field detection and large-range remote detection of a plurality of antigens with the existence of commercial antibodies.

A method for extracting mogroside and water-soluble dietary fiber free of pesticide residue simultaneously comprises the following steps that (1) momordica grosvenori is washed and juiced, pomace is rinsed, and an extraction solution is obtained; (2) boiling, cooling and centrifugation are performed, and centrifugate is obtained; (3) an effluent is collected by aluminum oxide and an activated carbon chromatographic column which are connected in series front and back; (4) ultrafiltration membrane filtration is performed, and a trapped fluid and a permeate liquid are collected respectively; (5) nanofiltration and concentration are performed: an ultrafiltration liquid is filtered by a nanofiltration membrane, the trapped fluid and the permeate liquid are collected respectively, a nanofiltration trapped fluid is concentrated and dried, and the mogroside free of pesticide residue is obtained; the nanofiltration trapped fluid and the ultrafiltration trapped fluid are combined, concentrated and dried, and the momordica grosvenori water-soluble dietary fiber free of pesticide residue are obtained; the mogroside and water-soluble dietary fiber products are free of pesticide residue, the content of glucoside V reaches 90.6%, and the yield reaches 96.3%; the purity of the water-soluble dietary fiber reaches 95%, and the yield reaches 91%; the method is simple, the operation is simple and convenient, and the method is suitable for industrialization production.

The invention pertains to the field of medical technology and provides triterpenoid saponin in camellia seeds, and a preparation method and medical application thereof. The triterpenoid saponin is shown in a general formula (1), wherein, R1 is hydrogen or oxhydryl or acyloxy; R2 is the hydrogen or the acyloxy; R3 is the hydrogen or the acyloxy; R4 is the hydrogen or the acyloxy; R5 is methyl or methylol or aldehyde group or carboxyl or metheyl carboxyl; R6 is the hydrogen or orglycosyl; the preparation method comprises the following steps: macroporous resins are degreased, extracted by ethanol, decocted in water, desugared and decolored to obtain crude total saponins and then go though an opened ODS chromatographic column and repeated HPLC method to obtain the total saponins of camellia seeds and monomer theasaponin. The triterpenoid saponin compounds in the camellia seeds have the functions of protecting gastric mucosa, being antineoplastic, reducing blood sugar and blood fat and thelike and are used for preparing medicaments or health-care foods having the functions of protecting gastric mucosa, being antineoplastic and reducing blood sugar and blood fat.

The invention discloses an analysis method for simultaneously measuring residues of 12 sulfonamide medicaments, 19 quinolone medicaments and 8 benzimidazole medicaments and metabolites thereof in chicken liver by quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and purification-ultrahigh performance liquid chromatography-tandem mass spectrometry (QuEChERS-UPLC-MS / MS). The method comprises the following steps of: extracting a sample by using 1 percent acetic acid-acetonitrile solution, purifying the sample by using NH2 adsorbent, and degreasing the sample by using n-hexane;and then performing separation by using a Kromasil Eternity C18 chromatographic column (100mm*2.1mm, 2.5mum), performing gradient elution by using 0.1 percent formic acid and methanol as mobile phases, performing ionization in an electron spray positive ion (ESI<+>) mode, performing detection in a multi-reaction monitoring (MRM) mode, and performing quantification by an internal standard method. The 39 medicaments have good linearity (r is more than 0.98) in a blank adding concentration range of 5 to 100mug / kg, the average recovery rate of the medicaments in the adding level range of 10 to 50mug / kg, the relative standard deviation (RSD) is 1.5 to 23.4 percent, the limit of detection (LOD) of the 39 medicaments is 5mug / kg, and the low limit of quantification (LOQ) is 10mug / kg. The method is simple, convenient, quick, sensitive, accurate and rugged, and is suitable for confirmation and quantitative determination of the residues of the residues of the sulfonamide, quinolone and benzimidazole medicaments in the chicken liver.

Rapid characterization and screening of polymer samples to determine average molecular weight, molecular weight distribution and other properties is disclosed. Rapid flow characterization systems and methods, including liquid chromatography and flow-injection analysis systems and methods are preferably employed. High throughput, automated sampling systems and methods, high-temperature characterization systems and methods, and rapid, indirect calibration compositions and methods are also disclosed. The described methods, systems, and devices have primary applications in combinatorial polymer research and in industrial process control.

During the extraction of resveratrol from giant knotwood, giant knotwood glycoside is hydrolyzed and enriched through organic synthesis. The extraction process includes mixing giant knotwood raw material and composite stuffing, high pressure chromatography in a high-pressure chromatographic column apparatus, gradient elution with chloroform and ethyl acetate and thin chromatographic tracking detection. The said process can reach a kilogram level yield and high product purity. The present invention may be used in extracting resveratrol and other similar product from giant knotwood material.

The invention relates to a fluorescence probe for detecting intracellular hydrogen sulfide and a preparation method and application of the fluorescence probe. Cy7 fluorochrome and sodium acetate are dissolved in anhydrous dimethylformamide (DMF), heating reflux is carried out for 2-4 hours under protection of Ar gas, cooling and suction filtration are carried out on an obtained mixture, mother liquor is crystallized under the low temperature of minus 4-20 DEG C, and keto form cyanine reddish brown crystals are obtained after the suction filtration; toluoyl benzoic acid and oxalyl chloride are dissolved in anhydrous CH2Cl2, then a bit of DMF is added, mixing is carried out for 2-6 hours under the temperature of 0 DEG C, and toluoyl benzoyl chloride is obtained; and keto form cyanine and triethylamine are dissolved in the anhydrous CH2Cl2 and are cooled to be 0 DEG C, the toluoyl benzoyl chloride is added dropwise, mixing is carried out for 10-30 minutes under the temperature of 0 DEG C, the mixture is heated to be in the room temperature, mixing is carried out overnight, suction filtration and spinning drying are carried out, separation is carried out through a chromatographic column, green solids are obtained after the spinning drying, and the structural formula is as follows. The fluorescence probe is simple in composition, high in detection speed, free of long-term incubation in a detection process, high in sensitivity, and capable of detecting intracellular endogenic hydrogen sulfide.

The present invention uses weak base phenylethylene series trialkylamine type anion-exchange resin as fixed phase and uses hydrochloric acid medium as mobile phone to implement coarse separation of platinum, palladium, iridium and gold from base metal, adopts dilute hydrochrolic acid, water and EDTA to elute base metal, and utilizes sulfocyanate+ascorbic acid solution, hydrochloric acid equilibrated methyl isobutanedione, NH4Cl+NH3.H2O solution and HCl+thiourea+thiourea+methyl alcohol solution to selectively shower-wash iridium, gold, palladium and platinum, and its chromatographic column canbe regenerated by using hydrochloric acid. Its resolution is good, the baseline separation can be obtained, and their recoveries are above 95%.

Disclosed herein are apparatus and methods for mitigating column bed compression over time. This invention provides for an improved column design and method of controlling bed density in a chromatographic column using a protruding portion of a filter assembly. The invention results in a higher sorbent bed density than can normally be achieved using a fixed length column of conventional design. The invention may be implemented as a unique filter assembly or as a column tailored for the application and a matching filter assembly.