The invention belongs to the technical field of artificial cultivation of medicinal fungi, and particularly relates to a method for cultivating ganoderma lucidum karst. The method includes the steps of obtaining ganoderma lucidum karst mother species, ganoderma lucidum karst protospecies and ganoderma lucidum karst cultispecies respectively, and conducting substitute cultivation of the ganoderma lucidum karst cultispecies to obtain ganoderma lucidum karst, wherein a cultispecies culture medium is prepared from corn straw, sawdust, pig manure, cow dung, rapeseed meal, humic acid, an amillariella mellea fermented liquid, a bacillus subtilis fermentation liquid and gypsum, and a culture medium of the substitute cultivation is same as the cultispecies culture medium. According to the method, the yield of ganoderma lucidum karst sporophore and conidial powder is significantly improved, and the contents of effective components, like ganoderma lucidum karst polysaccharide and ganoderma lucidum karst total triterpenoid, are significantly improved.