Method for cultivating ganoderma lucidum karst
A cultivation method and technology of Ganoderma lucidum, applied in the field of artificial cultivation of medicinal fungi, can solve the problems of long production cycle of fruit body cultivation, low content of biologically active substances, obstacles to mass production of Ganoderma lucidum, etc. The effect of bacterial contamination rate
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Embodiment 1
[0045] A cultivation method of Ganoderma lucidum, comprising the following steps:
[0046] 1) Inoculate Ganoderma lucidum (Leyss.: Fr.) Karst parent species into the parent species medium and culture at a constant temperature of 22-26°C for 5-7 days to obtain the parent species of Ganoderma lucidum. The mother seed medium includes the following components: 250g / L potato, 25g / L glucose, 3g / L beef extract, 6g / L peptone, and 15-20g / L agar powder.
[0047] 2) Inoculate the parent ganoderma seed into the original seed medium and cultivate it at a temperature of 20-22° C. and a humidity of 55-65% to obtain the original ganoderma seed. The original seed medium includes the following components by weight: 70 parts of sawdust, 6 parts of corncobs, 15 parts of wheat bran, 2 parts of brown sugar, 1 part of gypsum powder, KNO 3 0.25 parts; the water content of the original seed culture medium is 55-60%; the original seed culture medium is made into a 500mL original seed bottle. The pre...
Embodiment 2
[0055] A cultivation method of Ganoderma lucidum, the only difference from Example 1 is that the mother seed culture medium includes the following components: 200g / L of potato, 20g / L of glucose, 3g / L of beef extract, 4g / L of peptone, 15g of agar powder / L; the original seed medium includes the following components by weight: 60 parts of sawdust, 5 parts of corncobs, 10 parts of wheat bran, 1 part of brown sugar, 1 part of gypsum powder, KNO 3 0.05 parts; the water content of the original seed medium is 55-60%; the cultivar medium includes the following raw materials in parts by weight: 50 parts of corn stalks, 30 parts of sawdust, 10 parts of pig manure, 10 parts of cow dung, rapeseed 5 parts of meal, 5 parts of humic acid, 1 part of Armillaria fermented liquid, 1 part of Bacillus subtilis fermented liquid, 1 part of gypsum; the effective number of viable bacteria in the Armillaria fermented liquid is 1.0-2.0×10 6 / mL, the number of effective viable bacteria in the Bacillus s...
Embodiment 3
[0058] A cultivation method of Ganoderma lucidum, the only difference from Example 1 is: the Ganoderma lucidum is Ganodermasinense J.D.Zhao, L.W.Hsu et X.Q.Zhang. The average daily growth rate of mother species, original species, cultivated species, and cultivation bag mycelium can reach 18-20mm / d. It generally takes 30-45 days from primordia to fruiting body maturity, and can be harvested 1.5-2 months after cultivation; based on the dry weight of 1000kg of dry substitute material, 90-115kg of Ganoderma lucidum fruiting bodies can be harvested in the same year.
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