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755 results about "HeLa" patented technology
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HeLa (/ˈheɪlɑː/; also Hela or hela) is an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a patient who died of cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific, which warrants its extensive use in scientific research.
Biological compositions and methods for treatment of cervical cancer
The present invention relates to pharmaceutical compositions and dietary supplement comprising yeast cells that can produce a healthful benefit in a subject inflicted with cervical cancer. The biological compositions can be used to retard the growth of cervical cancer cells and / or prolonging the time of survival of the subject. The invention also relates to methods for manufacturing the biological compositions.
Owner:ULTRA BIOTECH
SgRNA combined with immunogene to inhibit high risk HPV expression, gene knockout vector thereof, and application of sgRNA and vector
ActiveCN105821040AImprove targetingGrowth inhibitionOrganic active ingredientsGenetic material ingredientsWilms' tumorCas9
The invention provides an sgRNA combined with immunogene to inhibit high risk HPV expression, a gene knockout vector thereof, and an application of the sgRNA and the vector. The sgRNA sequences of a human papilloma virus 16 type gene suitable for CRISPR-Cas9 targeting editing and a human PD-1 gene are selected, and the plasmid vector of the sgRNA and a CRISPR-Cas9 nuclease gene expression vector are transferred to HPV16+ human cervical carcinoma cells and HPV16+ transplantation tumor-bearing mice, and can obviously inhibit HPV16 expression and inhibit tumor growth. The gene expression vector prepared in the invention has the advantages of simple method steps, good sgRNA targeting property, high CRISPR-Cas9 knockout efficiency, accurate targeting splicing of high risk HPV16 type and PD-1 genes, efficient reduction of the expression of the high risk HPV16 type gene, and obvious inhibition of the tumor growth through combined application.
Owner:李旭
DNA detection and analysis method based on Cas9 nuclease and application thereof
ActiveCN108192956AImprove featuresHigh sensitivityMicrobiological testing/measurementNucleic acid detectionTyping
The invention provides a DNA detection and analysis method based on Cas9 nuclease and application thereof. The method comprises the following three steps: (1) carrying out PCR amplification on targetDNAs; (2) treating a PCR amplification product by using a CAT method; and (3) carrying out PCR amplification with DNAs treated by using the CAT method as a template. The method can successfully detectL1 and E6 / E7 genes of HPV16 and HPV18 in human cervical carcinoma cells. According to the invention, the method successfully avoids current key bottleneck problems in nucleic acid hybridization, designing of specific PCR primers and the like in the fields of nucleic acid detection and typing.
Owner:SOUTHEAST UNIV
Regulated genes in cervical cancer
Polynucleotides, as well as polypeptides encoded thereby, that are differentially expressed in SCCC cells are provided. The polynucleotides find use in diagnosis of cancer, and classification of cancer cells according to expression profiles. The methods are useful for detecting cervical cancer cells, facilitating diagnosis of cervical cancer and the severity of the cancer (e.g., tumor grade, tumor burden, and the like) in a subject, facilitating a determination of the prognosis of a subject, and assessing the responsiveness of the subject to therapy.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Penicillium citrinum sourced citrinin compound (penicitrinol O) as well as preparation method and application thereof
InactiveCN104478891AGood antitumor activityOrganic active ingredientsOrganic chemistryCitrininStructural formula
The invention relates to a penicillium citrinum sourced citrinin compound (penicitrinol O) as well as a preparation method and an application thereof. The compound has an effect on inhibiting tumor cell proliferation and has anti-tumor activity, and the structural formula is shown in the specification. The preparation method comprises the steps: fermenting and culturing penicillium citrinum IBPT-5 to obtain a fermented product; and then separating and purifying the fermented product to obtain the compound. The experiment shows that the compound has high anti-tumor activity for human cervical cancer cell line hela and can be used for preparing cell proliferation inhibition medicines or anti-tumor medicines for the study on anti-tumor.
Owner:FUZHOU UNIV
Liver cancer targeted peptide and application thereof
ActiveCN105039333AEfficient tumor targetingTumor targeting specificityRadioactive preparation carriersPeptidesTarget peptideElutriation
The invention discloses a targeted polypeptide capable of being specifically combined with tumors, particularly a targeted peptide capable of being specifically combined with liver cancer tissues and application thereof in diagnosis and treatment of liver cancer. The liver cancer targeted peptide is preferably HCC-47 of which the amino acid sequence is SQDIRTWNGTRS; and the liver cancer targeted peptide is specifically combined with the liver cancer tissues, and can not be specifically combined with cervical carcinoma cells Hela, mammary cancer cells MDA-MB231, kidney cancer cells CRL-1932 and lung cancer cells A549. The polypeptide is obtained by in-vitro biological elutriation by combining a bacteriophage display library and a living body cross sectioning technique. The polypeptide can be used in a molecular imaging preparation for early diagnosis of liver cancer. The polypeptide can also be used in targeted modification and preparation of drugs for treating liver cancer. The polypeptide can also be used for targeted modification on drug transport carriers, thereby providing a new way for diagnosing or treating patients with liver cancer.
Owner:TIANJIN MEDICAL UNIV
A macrocyclic oxidation substituted pentacyclic triterpanoids derivative and preparation method and use thereof
InactiveCN101117349AStrong inhibitory activityOrganic active ingredientsMetabolism disorderAlgluceraseAnti-Tumor Drugs
The present invention relates to a pentacyclic triterpanoid derivative of multiple-oxide substitution of the A ring and the medicine salt or solvate of the derivative, and the present invention also relates to the preparation method, the drug combination, and medical use of the derivative. The compound of the present invention has the functions of inhibiting the activity of six human tumor cell strains in vitro, such as human prostate cancer cell (PC-3), nasopharyngeal carcinoma cells (CNE), oral squamous carcinoma cell(KB), human lung cancer cell (A549), human hepatoma cell (BEL-7404), and human cervix cancer cell (Hela), and the function of the invention is at the same magnitude of the positive control of cisplatin, thereby the compound can be used as expected antitumor drug. The compound of the present invention also inhibits the alpha glucosidase strongly, and the inhibiting effect is greater than the positive control of acarbose, thereby the compound can be used as expected medicine for preventing and treating diabetes and the treatment of the virus diseases.
Owner:ZHEJIANG HISUN PHARMA CO LTD
Preparation method and time-resolved biological imaging application of thermally activated delayed long-life fluorescent organic material-based nanoparticles
ActiveCN105400507AStrong fluorescence emissionStable signalOrganic chemistryFluorescence/phosphorescenceStainingPolyethylene glycol
The invention discloses a preparation method and a time-resolved biological imaging application of thermally activated delayed fluorescent (TADF) organic material-based water-soluble long-life fluorescent nanoparticles. The nanostructure of the nanoparticles is represented by figure 1, a thermally activated delayed fluorescent molecule represented by CzPy is represented by a general formula (I). The method comprises the following steps: synthesizing the pure organic TADF micro-molecular material CzPy through adopting a one-step process, and rapidly injecting a CzPy / lecithin-polyethylene glycol mixed solution to deionized water to obtain the CzPy nanoparticles with good water solubility, high luminescence intensity and long fluorescence life. HELA cell dyeing marking and zebra blood vessel imaging results show that the nanoparticles have the advantages of long fluorescence life, low cytotoxicity and biotoxicity, stable spectrum signal, realization of time-resolved fluorescence imaging in cell or in vivo imaging, and good application prospect.
Owner:NANJING UNIV OF POSTS & TELECOMM
Preparation of anti cytoplasmic thymidine kinase-IgY and tumor diagnosis composition
InactiveCN1414017AHigh sensitivityEasy to useImmunoglobulins against animals/humansFermentationAntigenAmino acid
An anti-cytoplasm thymidine kinase-IgY antibody prepared from the C-terminal 31 peptide of human cervical cancer cell (HeLa)TK1, the application of the reagent kit prepared from said antibody in diagnosing tumor, the process for preparing said antibody and reagent kit, and its test method are disclosed.
Owner:SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
Conjugates of beta-carboline-3-carboxylic acid and oligopeptides, preparation, nano structure, and application thereof as antitumor agent
InactiveCN104211768AEnhanced inhibitory effectOrganic active ingredientsPeptidesPharmaceutical drugCarboxylic acid
The invention discloses conjugates of beta-carboline-3-carboxylic acid and oligopeptides, preparation, a nano structure, and an application thereof as an antitumor agent. The invention discloses conjugates (8a-f) of beta-carboline-3-carboxylic acid and six oligopeptides, wherein the six oligopeptides are Leu-Pro-Asn-Ile-Ser-Lys-Pro (LPNISKP), Arg-Gly-Asp-Ser (RGDS), Arg-Gly-Asp-Phe (RGDF), Arg-Gly-Asp-Val (RGDV), Leu-Asp-Ser (LDV), and Lys-Glu (KE). The invention discloses a preparation method and nano structure of the conjugates, discloses an inhibition effect of the conjugates on five tumor cell strains namely HL60, HCT-8, HeLa, BEL-7402, and HepG2, and further discloses an inhibition effect on tumor growth of S180 tumor-bearing mice. The experiment results show that the provided conjugates of beta-carboline-3-carboxylic acid and six oligopeptides have an excellent antitumor effect, so that the conjugates is advantageous for being used as an antitumor drug in clinic.
Owner:CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Polypeptide, polypeptide-siRNA (ribonucleic acid) induction coassembly and application thereof
ActiveCN108250267AGrowth inhibitionIncrease electropositivityOrganic active ingredientsPeptidesHemolysisMedicine
The invention provides polypeptide. The structural formula of the polypeptide is as shown below (the structural formula is shown in the description). The invention further provides a polypeptide-siRNAinduction coassembly which is composed of polypeptide and siRNA. The invention further provides application of the polypeptide-siRNA induction coassembly to cell carrying. The invention further provides application of the polypeptide-siRNA induction coassembly to preparation of drugs for treating cervical cancer. After polypeptide and target siRNA are mixed and hatched for 5 minutes at the room temperature, stable and uniform nano particles can be formed. A cytotoxicity test proves that the coassembly nano particles have high biocompatibility, and the cytotoxicity and hemolysis toxicity of the coassembly nano particles are both low. A cell proliferation resistant test further verifies that the polypeptide-siRNA nano particles can effectively block a cervical cancer cell line HeLa in the G2 stage, so that growth of the cervical cancer line HeLa is hindered.
Owner:PEKING UNIV SHENZHEN GRADUATE SCHOOL
Preparation of multi-epitope TK-1 antibody, and application of multi-epitope TK-1 antibody in evaluating treatment effect on tumor patient
ActiveCN102516390AHigh purityIncrease productionEgg immunoglobulinsTransferasesAntigenTreatment effect
The invention provides a high-specificity high-sensitivity coordinated compound anti-human-TK1 antibody prepared from an antigenic determinant composed of human cervical cancer cell TK1 monomer N-terminal 23 peptide, C-terminal 20 peptide and C-terminal 28 peptide, and application thereof in tumor diagnosis. The antigenic determinant contains the following amino acid sequences: N-terminal 23 peptide (3-25): CINLPTVLPGSPSKTRGQIQVIL, C-terminal 20 peptide (206-225) CPVPGKPGEAVAARKLFAPQ, and C-terminal 28 peptide (198-225) AGPDNKENCPVPGKPGEAVAARKLFAPQ. The invention also relates to a method for preparing the antibody prepared from the antigen. The antibody kit provided by the invention has the characteristics of high sensitivity, high specificity, low cost and the like. The treatment effect on the tumor patient is evaluated by an enhanced chemiluminescent point blotting detection method, immunohistochemical detection and the detection kit.
Owner:SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
Fly maggot extractive as well as preparation method and application thereof
ActiveCN101991608AEnhance the body's humoral immunitySimple and efficient operationAnthropod material medical ingredientsAntineoplastic agentsMaggotWater soluble
The invention discloses a fly maggot extractive containing the principal components of water-soluble proteins, and the water-soluble proteins contained in the fly maggot extractive have the mass percentage of 50-70 percent and the molecular weight of 2-16 KDa. The fly maggot extractive not only has obvious inhibiting effect on human promyelocytic leukemia HL-60 cells, human erythroleukemia K562 cells, human liver cancers SMMC-7721, mouse leukemia P388 cells, human lung adenocarcinoma A549 cells, human nasopharyngeal darcinoma CNE cells, human prostatic carcinoma PC3 cells, human cervical carcinoma HeLa in vitro, but also has outstanding inhibiting effect on mouse S180 sarcomas and mouse Heps liver cancer solid tumors, and also has the effect on enhancing the humoral immunity of organisms. The invention also discloses a preparation method of the fly maggot extractive. The preparation method is easy and convenient for operation and control and low in cost and is suitable for industrialized production.
Owner:浙江佰科堂生物科技股份有限公司
Surface-enhanced Raman technology based on signal-off and used for detecting intracellular telomerase activity
InactiveCN104611416ARealize highly sensitive detectionEnhanced Raman signalMicrobiological testing/measurementTelomeraseSignalling molecules
The invention relates to a surface-enhanced Raman technology based on signal-off and used for detecting intracellular telomerase activity. According to the technology, graphene, carbon nitride, MoS2, SiO2 and the like are taken as carriers, nano-gole or nano-silver with controllable dimension and morphology or a composite magnetic nano-particle with a core-shell structure is supported by the carriers, and then a hairpin probe containing a telomerase primer and a Raman molecule beacon is fixedly disposed on the nano=particle surface, so that the high-sensitivity high-selectivity SERS detection method based on signal-off is established. When a target exists, the primer extends and generates a DNA chain with a repeat sequence under the effect of telomerase, and the DNA chain is hybridized with the probe molecule, then the DNA hairpin structure is opened, a Raman signal molecule with the single-chain labeled end is far away from a Raman substrate, the Raman signal is reduced, and high-sensitivity detection on telomerase is realized. The probe prepared by taking Hela cervical carcinoma cell as a model realizes intracellular telomerase activity detection and dynamic detection on intracellular telomerase activity variation under effect of a telomerase inhibiting medicament. By using multiple cells for imaging, the probe is confirmed to be capable of distinguishing tumor cells and normal cells.
Owner:LINYI UNIVERSITY
Beta-hydroxy protected didecyl quaternary ammonium salt with anticancer activity and preparation method thereof
InactiveCN101967105AEfficient killingOrganic chemistryOrganic compound preparationCancer cellPharmaceutical Substances
The invention relates to the synthesis of an emodin derivative with anticancer function, in particular to beta-hydroxy protected didecyl quaternary ammonium salt with anticancer activity and a preparation method thereof. The didecyl quaternary ammonium salt is methyl didecyl-[2-(4,5-dihydroxy-7-methoxy-9,10-anthraquinonyl)methyl] ammonium salt and is prepared from emodin serving as a raw materialthrough a series of synthetic reactions. In the invention, the emodin derivative has proliferation inhibiting functions with different degrees for five cancer cells such as liver cancer HepG2, gastric cancer BGC, neuroma SH-SY5Y, gastric cancer AGS and cervical cancer Hela and the normal human embryonic lung fibroblast HELF, and can be used for preparing medicaments for treating cancers.
Owner:FUZHOU UNIV
Water-soluble perylene imide compounds, usage as DNA intercalator, and applications thereof in growth inhibition of cancer cells
InactiveCN103497154AHigh fluorescence quantum yieldGood water solubilityOrganic chemistryMicrobiological testing/measurementFluorescent imagingSolubility
The invention discloses water-soluble perylene imide compounds, a usage as a DNA intercalator, and applications thereof in the growth inhibition of cancer cells, and belongs to the technical field of chemical synthesis of biological drugs. Through inducing cation amino functional groups into perylene anhydride derivatives and a perylene mono-anhydride system, the water solubility of the perylene anhydride derivatives and the perylene mono-anhydride system is greatly improved; furthermore, the cation amino functional groups are in a photo-stability combination with perylene derivatives, and thus a series of novel water-soluble perylene imide compounds are obtained. The synthesized water-soluble perylene imide compounds can effectively be embedded between the base pairs of DNA double helix of nucleus and be used as a DNA intercalator. The synthesized water-soluble perylene imide compounds have a planar rigid structure, so that the compounds can be specifically enriched around fixed tissue cell nucleus, the compounds have the advantages of strong fluorescence and easiness in fluorescent imaging, and have a similar effect as the DAPI effect of commercial cell nucleus dye. The synthesized water-soluble perylene imide compounds can effectively inhibit the growth of cancer cells such as U2OS, HTC116, Hela, AGS, etc., and have a very prominent anti-tumor effect.
Owner:BEIJING UNIV OF CHEM TECH
Preparation of multi-epitope TK1 antibody and application of multi-epitope TK1 antibody to evaluation on recurrence risk and prognosis of tumor patient at early stage
ActiveCN102432683ATrue reflection of proliferationReflect proliferationEgg immunoglobulinsTransferasesAntigenCvd risk
The invention provides a high-specificity and high-sensitivity coordination combination anti-human TK1 antibody prepared from an antigenic determinant consisting of 23 peptide at an N end, 20 peptide at a C end and 28 peptide at the C end of human cervical cancer cells, and application thereof to tumor diagnosis. The antigenic determinant comprises the following amino acid sequences: 1) the 23 peptide (3-25) at the N end: CINLPTVLPGSPSKTRGQIQVIL: 2) the 20 peptide (206-225) at the C end: CPVPGKPGEAVAARKLFAPQ; and 3) the 28 peptide at the C end: AGPDNKENCPVPGKPGEAVAARKLFAPQ. The invention simultaneously provides a method applying the antigen for preparing the antibody. An antibody kit provided by the invention has the characteristics of high sensitivity, high specificity, low cost and the like, tumor rehabilitation populations are periodically monitored by an enhanced chemiluminescence dot blot detection method, immunohistochemistry detection and the detection kit, and the recurrence and transfer risk and the prognosis of the tumor rehabilitation people are evaluated.
Owner:SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
Water-soluble perylene bisimide compound and application of water-soluble perylene bisimide compound serving as DNA intercalator in anticancer cells and tumors
ActiveCN103936731AGood water solubilityGood chemical stabilityOrganic active ingredientsOrganic chemistryChemical synthesisSolubility
Owner:BEIJING UNIV OF CHEM TECH
Water soluble mitochondrial targeting imaging probe and preparation method thereof
ActiveCN105777637AGood water solubilityGood photobleaching resistanceOrganic chemistryIn-vivo testing preparationsHigh resistanceSolubility
The invention discloses a water soluble mitochondrial targeting imaging probe and a preparation method thereof. The structural formula of compounds that form the water soluble mitochondrial targeting imaging probe can be formula (I), (II), (III), (IV), (V), or (VI). The water soluble mitochondrial targeting imaging probe has the characteristics of good water solubility, high resistance to photo-bleaching, high thermal stability, low toxicity, and high sensitivity. Hela, MCF-7, and NIH-3T3 cells are taken as the models to observe the distribution situation of the fluorescent probe in cells through a laser con-focal microscopy technology; the results show that the probe is mainly distributed in mitochondrial, and the targeting property is good. The test results show that the provided probe has the characteristic of strong mitochondrial targeting imaging performance on cancer cells and non-cancer cells; thus an effective means is provided for quantitatively research superoxide radicals in cells in time and space, and thus the pathologic and physiological mechanisms of active oxygen radicals can be researched.
Owner:INST OF CHEM CHINESE ACAD OF SCI
Anti-cervical cancer effect of oleanolic acid and pharmaceutical preparation thereof
InactiveCN102133219AEnhanced inhibitory effectNo obvious side effectsOrganic active ingredientsAntineoplastic agentsCancer cellSide effect
The invention discloses a new medical application of oleanolic acid in cervical cancer resistance, and also discloses clinically acceptable pharmaceutical preparations such as tables, capsules, pills, injections and the like which are prepared by using oleanolic acid as a pharmaceutical raw material. The oleanolic acid pharmaceutical preparation of the present invention comprises 1%-99% of oleanolic acids and 99%-1% medicinal excipients (including other compatible drugs). The oleanolic acid and the pharmaceutical preparations thereof provided by the invention have an excellent anti-cervical cancer effect which is represented by significant inhibitory activity against in vitro human cervical cancer cells and significant tumor inhibitory activity against a transplanted tumor in nude mice of the cancer cell. In addition, the active components of the pharmaceutical preparation are traditional Chinese medicine extracts, so the preparation has the advantage of less toxic and side effect than chemotherapeutic drugs.
Owner:林秀坤
Application of cell nucleus targeting chitosan-fatty acid graft as medicine carrier micelle
InactiveCN101066461ALow carrier toxicityLow toxicityPharmaceutical non-active ingredientsAntineoplastic agentsLysosomeCell membrane
The present invention is application of medicine carrying chitosan-fatty acid grafting micelle in preparing targeting antitumor medicine carrier, especially antitumor medicine carrier targeting to lung cancer cell nucleus, cervical carcinoma cell nucleus and mammary cancer cell nucleus. The chitosan-fatty acid grafting micelle of the present invention has grafting carrier with low toxicity, and may be ingested fast by cell nucleus to target to cell nucleus and to raise the curative effect of antitumor medicine by up to 80 times. It has cell nucleus targeting function of permeating cell membrane fast with less destruction by intracellular lysosome. The grafting micelle with hydrophobic kernel can envelop hydrophobic antitumor medicine targeting cell nucleus to form medicine carrying grafting micelle. The present invention provides nanometer subcellular organelle administration for high efficiency low toxicity tumor treatment.
Owner:ZHEJIANG UNIV
Nanopipette device and method for subcellular analysis
ActiveUS20160032275A1Immobilised enzymesBioreactor/fermenter combinationsChemical treatmentScanning ion-conductance microscopy
Described herein are devices and methods for extracting cellular material from living cells and then depositing them into to a receptacle in a nanoliter scale. Using a nanopipette integrated into a scanning ion conductance microscope (SICM), extraction of mitochondrial DNA from human BJ fibroblasts and Green Fluorescent Protein (GFP) transcripts from HeLa / GFP cells was achieved with minimal disruption to the cellular milieu and without chemical treatment prior to obtaining the isolated sample. Success of the extraction was confirmed by fluorescence microscopy and PCR analysis of the extracted material. The method and apparatus may be applied to many different cell types and intracellular targets, allowing not only single cell analysis, but single subcellular compartment analysis of materials extracted in their native state.
Owner:RGT UNIV OF CALIFORNIA
Biomarkers for human papilloma virus-associated cancer
ActiveUS20090136486A1Microbiological testing/measurementAntibody ingredientsSynaptonemal complex protein 2Antigen
Cervical cancer cells and HPV+ head and neck cancer cells express three testis-specific genes not normally expressed in somatic cells: testicular cell adhesion molecule 1 (TCAM1), synaptonemal complex protein 2 (SYCP2) and stromal antigen 3 (STAG3). Among the three markers, TCAM1 and SYCP2 are early detection markers. Various methods for identifying a human or non-human animal as a candidate for further examination for cervical cancer, preneoplastic lesion for cervical cancer, head and neck cancer, or preneoplastic lesion for head and neck cancer are disclosed. Methods of detecting said cancers and preneoplastic lesions, methods of screening for drugs for treating said cancers and preneoplastic lesions, methods for monitoring the effectiveness of a treatment for said cancers, and methods of treating said cancers are also disclosed. Further disclosed are kits that can be used to practice the above methods.
Owner:WISCONSIN ALUMNI RES FOUND
Fructus terminaliae billericae extract with anti-cancer effect, and preparation method of effective part of fructus terminaliae billericae extract
ActiveCN109453212AHigh anticancer activitySimple production processAntineoplastic agentsPlant ingredientsEnrichment methodsIn vivo
The invention relates an anticancer active part of fructus terminaliae billericae, and a preparation method of the anticancer active part. The invention comprises extraction of the part containing active components, study of a process for enriching by adopting macroporous absorption resin, identification of the active components in the active part, and use of the active part in in-vivo or in-vitroinhibition of liver cancer HepG2, lung cancer A549, lung adenocarcinoma NCI-H1703, gastric cancer BGC823, osteosarcoma cell MG-63, colorectal cancer HCT116, breast cancer MCF-7, neuroblastoma cells shsy5y, kidney cancer ACHN, normal liver cells L02, human breast ductal carcinoma cells ZR75-1, human colorectal adenocarcinoma cells Colo-205, human breast ductal carcinoma cells BT-474, human breastcancer cells T-47D, human cervical cancer cell line HeLa, liver cancer cells H22, and the like. The enrichment method applying the macroporous absorption resin to the anti-cancer active part of the fructus terminaliae billericae is simple in process, safe, non-toxic and low in production cost, and can be used for industrial production, thus having a great economic benefit and higher generalizationperformance.
Owner:BEIJING UNIV OF CHINESE MEDICINE
METHOD FOR INHIBITION OF TUMOR CELL GROWTH USING (22R)-5alpha-LANOSTA-8,24-DIEN-3Beta,15alpha,21-TRIOL
ActiveUS20120309732A1Good treatment effectReduced tumor cell growth rateOrganic active ingredientsPeptide/protein ingredientsHuman leukemiaCytotoxicity
In this patent, we isolated a novel compound from fruiting body of Antrodia cinnamomea, namely, (22R)-5α-lanosta-8,24-dien-3β,15α,21-triol. This compound possesses preferential cytotoxicity against human leukemia, pancreatic cancer, esophageal cancer, hepatoma, and cervical cancer cells.
Owner:CHEN YU JEN
Ursolic acid derivative with anti-cancer activity and preparation method thereof
InactiveCN102516351AGood antitumor effect in vitroTargetedSteroidsAntineoplastic agentsUrsolic acidEthylenediamines
The invention provides an ursolic acid derivative, which is N-[3beta-acetoxy-ursa-12- alkene-28-acyl]-aminoethylenediamine-folic acid. A natural product ursolic acid is structurally transformed to obtain N-[3beta-acetoxy-ursa-12- alkene-28-acyl]-aminoethylenediamine-folic acid (I), N-[3beta-acetoxy-ursa-12-alkene-28-acyl]-aminoethylenediamine (III) and N-[3beta-acetoxy-ursa-12- alkene-28-acyl]-aminoethylenediamine (IV). The in vitro cell proliferation inhibition test shows that the proliferation inhibition effect of the compounds I, III and IV on tested tumor cell lines HepG2, of BGC823, A-375, Hela and the like is superior to that of the ursolic acid, and the toxic effect of the compounds I, III and IV on human embryonic lung fibroblasts (HELF) is lower than that of the ursolic acid. The in vitro anti-tumor effect of the compound III is the best, and the compound I has certain targeting.
Owner:FUZHOU UNIV
Folic acid-benzaldehyde nitrogen mustard-HPMA macromolecule copolymer and preparation and application thereof
InactiveCN103923256AEnhanced inhibitory effectExtended stayOrganic active ingredientsPharmaceutical non-active ingredientsMethacrylamideBenzaldehyde
The invention provides a folic acid-benzaldehyde nitrogen mustard-HPMA macromolecule copolymer, which is prepared by bonding folic acid and benzaldehyde nitrogen mustard to N-(2-hydroxypropyl) methacrylate (HPMA) by virtue of covalent bonds and belongs to the field of macromolecular synthesis. According to the folic acid-benzaldehyde nitrogen mustard-HPMA macromolecule copolymer, antineoplastic activity of folic acid, benzaldehyde nitrogen mustard and N-(2-hydroxypropyl) methacrylate are overlapped, so that the inhibiting effect of the polymer on tumor is further improved, and the standing time of anti-cancer medicines on the tumor is greatly prolonged. An experiment proves that the copolymer has a function of targeting intelligent drug release and has the good inhibiting effect on cervical cancer cell HeLa; moreover, because of HPMA, the toxicity of anti-cancer medicines is also reduced, and the high biocompatibility is represented, so that the damage to normal tissues is reduced; therefore, the polymer has good prospect when the polymer used as an HeLa tumor cell inhibiting agent is applied to preparation of anti-tumor medicines.
Owner:NORTHWEST NORMAL UNIVERSITY
Application of iridium complex in preparing mitochondria targeted anti-tumor drugs
InactiveCN108635357AInduce apoptosisEnhanced inhibitory effectOrganic active ingredientsAntineoplastic agentsHuman tumorPhenanthroline
The invention discloses application of an iridium complex in preparing mitochondria targeted anti-tumor drugs. The structural formula of the iridium complex is [Ir(ppy)2(HPIP)]C1, wherein ligand represented by ppy is 2-phenylpyridine, ligand represented by HPIP is 3-(1H-imidazole[4,5-f][1,10]phenanthroline-2-yl)phenol. The iridium complex has the high inhibiting effect on human tumor cells HeLa, HepG2, A-549 and a cisplatin resistant strain CP / R-A549, mitochondria in tumor cells can be stored up, tumor cell apoptosiss can be induced, and the iridium complex can serve as the new anti-tumor drugtaking tumor cell mitochondria as target sites.
Owner:GUANGDONG PHARMA UNIV
Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs
InactiveCN104861085AImproves Structural IntegrityStrong inhibitory activityOrganic active ingredientsAntineoplastic agentsHomopolysaccharideSide effect
The invention provides a chestnut seed alpha-1,6-glucan, a preparation method for the chestnut seed alpha-1,6-glucan, and application of the chestnut seed alpha-1,6-glucan to anticancer drugs. The structural formula of the chestnut seed alpha-1,6-glucan is [arrow 6)-alpha-Glcp(1 arrow]n, the weight average molecular weight is in the range of 1600-2400 kDa, and the average molecular weight is 2000 kDa; the chestnut seed alpha-1,6-glucan belongs to homopolysaccharides, and has obvious inhibited activity on proliferation of cervical cancer cells HeLa, breast cancer cells MCF-7 and adriamycin-resistant human breast cancer cell MCF-7 in vitro; chestnut is a rare fruit with the edible Chinese herbal function, and has no serious toxic and side effect; the chestnut seed alpha-1,6-glucan obtained according to the preparation method provided by the invention is high in sugar chain structural integrity, and is expected to be developed into anticancer drugs or assisted anticancer drugs.
Owner:HEBEI UNIVERSITY
Preparation of organic integral small column, organic integral small column and application of organic integral small column
InactiveCN107297086AEfficient enrichment abilityQuick analysisComponent separationSolid sorbent liquid separationPoly methacrylateRadical polymerization
The invention relates to preparation of a Ti(IV)-IMAC organic integral small column. The Ti(IV)-IMAC organic integral small column is applied to phosphorylated proteomic analysis of trace biological samples. The organic integral column material is a polymethacrylate high polymer, contains a rich pore structure and is large in specific surface area and large in quantity of titanium ion enriching active sites; and meanwhile, due to a hydrophilic material surface, the organic integral column shows powerful enriching capability in the aspect of phosphopeptide enrichment. By applying the organic integral small column to the phosphorylation proteomic analysis of 5[mu]g HeLa cells, 1,000 or more of non-redundant phosphorylation sites are identified on an average, and the enriching specificity reaches up to 92.5%. More importantly, the Ti(IV)-IMAC organic integral small column is simple in preparation, and compared with packed small columns, the Ti(IV)-IMAC organic integral small column can be prepared through a photo-induced free-radical polymerization reaction by a few minutes only; and preparation of plugs is not required, so that the preparation time for an enriching material is reduced to a large extent.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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