1000 results about "Hemolysis" patented technology

Embodiments of the present invention relate generally to certain types of reciprocating positive-displacement pumps (which may be referred to hereinafter as “pods,”“pump pods,” or “pod pumps”) used to pump fluids, such as a biological fluid (e.g., blood or peritoneal fluid), a therapeutic fluid (e.g., a medication solution), or a surfactant fluid. The pumps may be configured specifically to impart low shear forces and low turbulence on the fluid as the fluid is pumped from an inlet to an outlet. Such pumps may be particularly useful in pumping fluids that may be damaged by such shear forces (e.g., blood, and particularly heated blood, which is prone to hemolysis) or turbulence (e.g., surfactants or other fluids that may foam or otherwise be damaged or become unstable in the presence of turbulence).

A method of inspecting a clinical specimen for a presence of one or more interferents, such as those that might be found within clinical analytical blood specimens by subjecting the specimen to centrifugation to separate the specimen into a red blood cell portion and a blood serum or plasma portion is provided. Subsequent to the centrifuging procedure, the serum or plasma portion of the clinical analytical specimen may be tested for the presence of one or more interferents such as hemolysis, icterus, lipemia, or liquid nonuniformities therein. Additionally, physical dimensional characteristics of the sample container and / or specimen may be determined. Apparatus for carrying out the method are described, as are other aspects.

A hysteroscopic fluid management system includes a saline source with an electrolyte concentration, at least one pressure mechanism for circulating saline to and from a targeted site and through a filter having filter characteristics back to the source, and a controller. The controller provides a saline inflow in a first flow path to the site and a saline outflow in a second flow path from the site through the filter and back to the source at a controlled flow rate. A diagnostic or therapeutic procedure is performed at the site in the presence of the saline. The filter characteristics and the controlled flow rate are selected to (1) cause substantially no change in the electrolyte concentration in the saline, (2) to prevent hemolysis of greater than 5% of filtered red blood cells exposed to the saline, and / or (3) to minimize effect on prothrombin time of plasma exposed to the filter.

The present invention provides an aqueous composition for storage of red blood cells consisting essentially of: adenine; dextrose; at least one non-metabolizable membrane-protectant sugar; and a specifically defined pH buffering system. Also provided are improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions.

The invention provides a method and apparatus for performing selective hypothermia to the brain and spinal cord for injury protection without the need for systemic cooling. A flexible catheter is inserted into the cerebral lateral ventricle or spinal subdural space. The catheter has lumens with a heat transfer element. The lumens of the catheter circulate a coolant and communicate at the distal heat transfer element for transfer of heat from the cerebrospinal fluid. Furthermore a method of maintaining catheter patency and providing blood clot hemolysis and drainage is also provided through the use of ultrasonic and / or laser energy delivered through the catheter.

Methods of treating patients having susceptible viral infections, especially chronic hepatitis C infection by administering to said patient a therapeutically effective amount of a combination therapy of interferon-alfa and ribavirin for a time sufficient to lower HCV-RNA in association with a therapeutically effective amount of an antioxidant for a time sufficient to ameliorate ribavirin-related hemolysis are disclosed.

The invention provides a method and apparatus for performing selective hypothermia to the brain and spinal cord for injury protection without the need for systemic cooling. A flexible catheter is inserted into the cerebral lateral ventricle or spinal subdural space. The catheter has lumens with a heat transfer element. The lumens of the catheter circulate a coolant and communicate at the distal heat transfer element for transfer of heat from the cerebrospinal fluid. Furthermore a method of maintaining catheter patency and providing blood clot hemolysis and drainage is also provided through the use of ultrasonic and / or laser energy delivered through the catheter.

An apparatus and method for spectroscopic measurement of an analyte in a sample is provided. The apparatus comprises a source of electromagnetic radiation (EMR) producing a light path, an aperture located within the light path and between the EMR source and a sample slot, and a photodector. The apparatus also has a primary calibration algorithm that is in operative association with the spectroscopic apparatus. Examples of analytes that may be measured using this apparatus include, but are not limited to Total-Hemoglobin, Met-Hemoglobin, Hemoglobin-based blood substitutes and any Met-Hemoglobin equivalent. The measurement of Met-Hemoglobin may be used to provide an accurate measurement of Total-Hemoglobin in whole blood, or Hemoglobin when used as an indicator of hemolysis. The measurement of Met-Hemoglobin may also be also used as a means of monitoring the degradation or reversal of degradation of Hemoglobin-based blood substitutes, or as a means of monitoring the oxidation or reversal of oxidation of Hemoglobin to Met-Hemoglobin.

A non-occluding intravascular pump comprises a shroud providing an inlet for incoming blood flow and an outlet for outgoing blood flow, wherein the shroud is a cylindrical housing; an impeller positioned within shroud, wherein a central axis of the shroud and impeller are shared; a motor coupled to the impeller, wherein the motor rotates the impeller to causes blood to be drawn through the inlet and output to the outlet, and the motor is centrally disposed and shares the central axis with the shroud and the impeller; and a plurality of pillars coupling the motor to the shroud, wherein the pillars secure the shroud in close proximity to the impeller. Various design features of the pump may be optimized to reduce hemolysis, such as, but not limited to, inlet length, impeller design, pillar angle, and outlet design.

The invention provides methods for preparing pharmaceutical formulations for injection such that upon injection the formulation does not cause erythrocyte agglutination, hemolysis, and / or cell shrinkage. To prevent agglutination, a pharmaceutical formulation ready for injection needs to have a sufficient ionic strength. To prevent hemolysis or cell shrinkage, a pharmaceutical formulation ready for injection needs to be about isotonic with respect to plasma. The invention provides methods that prepare pharmaceutical formulations for injection that have both the sufficient ionic strength to prevent agglutination and the requisite tonicity to prevent significant hemolysis or cell dehydration or shrinkage. The present methods involve the use of sodium chloride solutions that are about 25 mM to about 150 mM for reconstituting lyophilized cakes (or other non-liquid pharmaceutical formulations) into solution or for diluting pharmaceutical formulation solutions.

A plasma or serum separation membrane capable of omitting centrifugation, without hemolysis caused by red blood cell destruction, capable of conveniently and quickly separating plasma or serum from blood; and a filter comprising a plasma or serum separation membrane. In particular, a plasma or serum separation membrane is a membrane that separates plasma or serum from blood and has a void ratio of 30% or less; and a filter that includes a filter element capable of moving plasma faster than blood cells And the plasma or serum separation membrane sequentially connected with the rear side of the filter element.

A tubing set for use with a blood handling system is described that decreases the priming volume and surface area of the blood handling circuit, lowering the risk of hemodilution, platelet activation, and hemolysis. The tubing set also reduces the number of manual connections required, and uses quick-disconnect couplings to facilitate connections between the various lines, and between the lines and the arterial and venous cannulae.

An apparatus for separating plasma by which plasma can be separated from a small amount of whole blood cell sample without centrifugation is disclosed. This apparatus includes a blood channel through which blood flows; and a plasma channel through which plasma separated from said blood flows. The plasma channel is arranged at least partially in parallel with said blood channel and the blood channel and the plasma channel are at least partially in contact with each other along the longitudinal direction of the channels. Blood is made to flow at a flow rate at which blood cell components in the blood flowing through the blood channel axially accumulate and at which hemolysis does not occur. The plasma moves to the plasma channel after being separated into a blood cell layer and a plasma layer.

The invention relates to a method for preparing clindamycin phosphate powder filling agent, including: adding and dissolve clindamycin phosphate in alcohol solution; adding in activated charcoal to make decolorization, then roughly filtering, finely filtering, and placing to crystallize, filtering to eliminate supernatant and obtaining clindamycin phosphate crystal; then making transfer-solution for the second time, recrystallizing once, filtering and obtaining the recrystallized clindamycin phosphate crystal; drying and crushing, making split charging, capping and packaging in aseptic condition and making it. In the preparing course, it needs no high temperature treatment, need not add in additive, and its powder has good fluidity, high purity, few impurities, high bio-capability and good stability. It has no anaphylaxis and hemolysis and has no stimulation to blood vessels by intravenous injection. It adds a new clinic form of clindamycin phosphate, meeting clinic requirement.

A quality control material is disclosed which is used to monitor the calibration or used for recalibration of instruments used to screen for interferents in serum or plasma specimens. In particular, the quality control material disclosed is used to monitor instrument calibrations or used for recalibration for instruments which assess the amount of hemolysis, turbidity, bilirubinemia and biliverdinemia, either separately, or any two, or any three, or all four simultaneously, in plasma or serum samples. The quality control material does not contain any blood products such as plasma lipids, bile pigments, or hemoglobin, is stable at room temperature, and is ready for use with up to four constituents.

The present invention relates to injectable formulations of irritant agents, such as calcimimetics, that are pharmaceutically stable and demonstrate a reduced incidence of irritation, pain, phlebitis, precipitation and hemolysis upon injection.

The invention relates to a method for differentially hemolyzing whole blood. It discloses method for detecting an analyte in a liquid sample known or suspected to comprise red blood cells and suspected or known to comprise eukaryotic cells, the method comprising the steps of processing said liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may comprise both erythrocytes as well as nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.

The present invention provides an aqueous composition for storage of red blood cells consisting essentially of: adenine; dextrose; at least one nonmetabolizable membrane-protectant sugar; and a specifically defined pH buffering system. Also provided are improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions.

The invention is directed to antimicrobial peptides related to cyclic and short peptides (less than 10 amino acid residues) with unique patterns of aromatic and cationic residues that perform a wide range of antimicrobial activities but display low hemolysis.

The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.

The invention discloses a method for fixing heparin on polylactic acid hemodialysis membrane surface. Poly lactic acid has good biocompatibility, but has a certain of hydrophobicity, and hemodialysis membrane materials are often required to have a certain of hydrophilicity to reduce adsorption of protein and blood cells and prevent blood coagulation, so that hydrophilic modification is needed. Through use of the method of the invention, polylactic acid hemodialysis membrane surface can be modified through self-polymerization and attachment behaviors of dopamine, the heparin is covalently fixed on the membrane surface through a further reaction with heparin, and then a polylactic acid hollow fiber hematodialysis membrane having anticoagulation effect and good hydrophilicity is prepared. In the method, through utilization of the oxidation, self-polymerization, crosslinking adhesive properties of the dopamine, the heparin can be fixed on the membrane material surface. The prepared polylactic acid hollow fiber membrane has good hydrophilicity, biocompatibility, less adsorption of proteins and platelets, is not easy to cause hemolysis, and has more mild conditions than that of the prior art modification methods and obvious effects.

The invention relates to the technical field of medical diagnostics, in particular to a small molecule metabolite map for identifying liver cancer, hepatitis or liver cirrhosis and a manufacturing method thereof. The small molecule metabolites are four blood metabolites markers, i.e. palmitoyl hemolysis phosphoric acid ethanolamine glyceride, docosapentaenoic acyl hemolysis phosphatidyl choline, docosahexaenoic acyl hemolysis phosphatidyl choline and taurocholic acid, which respectively have the molecular weight of 453.2855, 569.3481, 567.3319 and 516.2916, and the corresponding ions of 454.2928, 570.3547, 568.3391 and 480.2776 detected through mass spectrum. Through experimentation on animals, the accuracy for determination of the liver cancer phase of a rat is 85 percent, and the accuracy for determination of the hepatitis or the liver cirrhosis is 94 percent. Clinical experiment results show that the accuracy for determination of the liver cancer is 85 percent, and the accuracy for determination of non-liver cancer is 71.9 percent. The method provided by the invention has the advantages of high sensitivity and high throughput, is superior to the existing liver cancer single diagnosis marker, and is applicable to screening and assisted diagnosis of the liver cancer.

The present invention discloses one set of modified antibacterial peptides and their preparation process and application. The modified antibacterial peptides include 37 modified antibacterial peptides, i. e., rLL-37-1 to rLL-37-17 antibacterial peptides, rLL-24-1 to rLL-24-8 antibacterial peptides, rLL-18-1 to rLL-18-2 antibacterial peptides and rLL-30-1 to rLL-30-10 antibacterial peptides. The present invention also provides serial negatively charged protein carriers for preparing the modified antibacterial peptides. The modified antibacterial peptides are applied in medicine, can reduce hemolysis and other side reactions, and may be prepared easily.

The present invention provides an automatic analyzer making it possible to reduce the cost for serum information measurement and prevent a decrease in processing capacity. An automatic analyzer comprises a colorimetric analysis section provided with a reaction vessel to mix a sample with a reagent and a measurement section to measure the color change of the mixture in the reaction vessel; and an electrolyte measurement section to measure the concentrations of electrolytes in a sample, wherein the sample included in the reaction vessel is diluted to be measured in the measurement section of the colorimetric analysis section, and at least one of the degrees of hemolysis, lipemia and icterus in a sample is measured in the electrolyte measurement section.

An intravenous system can be optimized to improve blood draw success and reduce hemolysis within the blood sample. Multiple optimizations can be made to an intravenous system, such as a peripheral intravenous catheter, to enhance the system's ability to provide blood samples having sufficient quality for many different tests. These optimizations can include features which enable an intravenous system, such as a peripheral intravenous catheter, to continue to perform efficiently when used to obtain blood samples even after the system has been placed within the patient's vasculature for a substantial duration of time. Also, these optimizations can include features for optimizing the fluid path and flow characteristics during blood withdrawal to minimize the amount of hemolysis that may be caused during withdrawal. Further, these optimizations can include features for integrating blood acquisition and dispense capabilities within the system.

An implantable cardiovascular blood pump system is provided, suitable for use as a left ventricular assist device (LVAD) system, having an implantable cardiovascular pump, an extracorporeal battery and a controller coupled to the implantable pump, and a programmer selectively periodically coupled to the controller to configure and adjust operating parameters of the implantable cardiovascular pump. The implantable cardiovascular blood pump includes a coaxial inflow cannula and outflow cannula in fluid communication with one another and with a pumping mechanism. The pumping mechanism may be a vibrating membrane pump which may include a flexible membrane coupled to an electromagnetic actuator assembly that causes wavelike undulations to propagate along the flexible membrane to propel blood through the implantable cardiovascular pump. The implantable cardiovascular pump may be programmed to operate at frequencies and duty cycles that mimic physiologic flow rates and pulsatility while avoiding thrombus formation, hemolysis and / or platelet activation.

The present invention discloses ibuprofen amino acid salt injection and a preparation method thereof. In the present invention, sodium bicarbonate is used as preparation auxiliary material, which ensures the prepared injection has stable quality, little stimulation, no hypersusceptibility effect, no hemolysis effect and stable curative effect and takes effect fast. The present invention is mainly applied to antipyresis and acesodyne under the circumstance which is not fit for oral medication or is not capable of the oral medication.

The invention belongs to the field of microbes, and concretely relates to a lactic acid bacterium with wide-spectrum bacteriostatic activity and application thereof. The lactic acid bacterium with wide-spectrum bacteriostatic activity is named lactobacillus salivarius GZPH2, is preserved at China Center for Type Culture Collection (CCTCC) since 27th, November, 2014, and has the preservation number of CCTCC M 2014598. The provided lactic acid bacterium lactobacillus salivarius GZPH2 has good bacteriostatic effect on multiple pathogenic bacteria of human and aquaculture bionts, and possesses the characteristics of not generating histamine, no hemolysis, acid resistance, cholate resistance and the like.

The invention is directed to a multi-disk centrifugal pump suitable for pumping fluids, including biocompatible fluids, such as blood. The design of the pump components is optimized to reduce or eliminate the probability of clotting and hemolysis when pumping blood, and to be energy efficient and resistant to physical shock for portable applications.