130 results about "Antibiotic peptide" patented technology

The invention provides a lactobacillus plantarum ZJ316 which is separated from excrement of infants and anti-bacterial peptide which is produced by the fermentation of the lactobacillus plantarum ZJ316, namely plantaricin ZJ316, and preparation and application thereof; the plantaricin ZJ316 is a novel lactobacillus anti-bacterial peptide with high efficiency and small molecular; the anti-bacterial peptide has bacteriostatic action of broad spectrum; due to small molecular weight, a lipid bilayer is hard to be crossed so as to form a complex body with small holes to be different from the action mode of nisin; but the anti-bacterial peptide also has sterilizing capacity with high efficiency, is a lantibiotic anti-bacterial peptide with superhigh application and development potential and can be applied to preparing the alternative medicine of feed additives, food preservatives and antibiotic, etc.

The invention discloses a preparing method of east Asia Tityus antibiotic peptide gene and appliance, which comprises the following steps: restructuring bacillus coli Escherichia coli DH5a / BmKAMP1, CCTCC NO: M207036; constructing east Asia Tityus ioterium cell cDNA library; choosing PCR method; sieving positive colony of scorpion antibiotic peptide gene from ioterium cDNA library; sequence-analyzing coding trait of antibiotic peptide gene; assuring amino acid sequence of the antibiotic peptide gene; adopting chemosynthesis antibiotic peptide; possessing inhibition of diverse density for Gram's bacterium. This antibiotic peptide possesses specificity and high active, which can be used as antibacterial drugs.

The invention relates to a method for preparing silk fibroin film, especially a method for using covalence decoration to prepare novel antibiosis silk fibroin film. The inventive method comprises that removing foreign materials of Na2CO3 solution smelted silk fibroin, dissolving in lithium bromide water solution, drying, mixing alcohol with water, immerging in phosphate buffer solution, immerging in EDC HCl / NHS solution, immerging and washing in phosphate buffer solution, drying. The invention can adjust the density of silk fibroin, the density of antibiosis peptide solution and process covalence decoration, to control the antibiosis function. The invention has the structure and functions of natural animal silk fibroin, with surface antibiosis activity, long service life and non drug tolerance.

The invention relates to a peptide or peptide derivative having the general formula: Sub1-X1-D2K3-P4-P5-Y6-L7-P8-R9-P10-X2-P12-P13-R14-X3-I16-P17 / Y17-N18-N19-X4-Sub2, wherein X1 is a non-polar, hydrophobic group or a positively charged group, D2 is asparagine or glutamine, K3, X2, and X4 are positively charged groups, X3 is a positively charged group, proline, or a proline derivative; L7 and I16 are non-polar, hydrophobic groups, Y6 and Y17 are tyrosine, R9 and R14 are arginine, N18 and N19 are asparagine or glutamine, P4, P5, P8, P10, P12, P13, and P17 are proline, hydroxyproline, or derivatives thereof, wherein possibly one or two of the groups selected from D2, P4, P5, P8, P10, P12, P13, P17, and Y17 are replaced by an arbitrary group and / or P13 and R14 are exchanged, Sub1 is the free or modified N-terminus, and Sub2 is the free or modified C-terminus. The invention further relates to the use of the peptides and peptide derivatives in medicine, as an antibiotic, in a disinfectant or cleaning agent, as a preservative or in a packaging material, in pharmaceutical research, or in a screening method.

The invention discloses a coated fabric containing antibacterial peptides and the preparation method, the surface of the fabrics are coated with coating agents containing antibacterial peptides. The preparation method comprises as follows: firstly, the antibacterial peptides are dissolved or dispersed into the coating agents at normal temperature to form finishing agents, then the fabrics are coated by the conventional rolling-baking method. The coated fabric containing antibacterial peptides has the advantages of broad spectrum antibacterial and bactericidal effect because the surface of the fabrics are coated with coating agents containing antibacterial peptides; no obvious influence on the appearance of the fabrics due to the thin coating layer.

The present invention discloses one set of modified antibacterial peptides and their preparation process and application. The modified antibacterial peptides include 37 modified antibacterial peptides, i. e., rLL-37-1 to rLL-37-17 antibacterial peptides, rLL-24-1 to rLL-24-8 antibacterial peptides, rLL-18-1 to rLL-18-2 antibacterial peptides and rLL-30-1 to rLL-30-10 antibacterial peptides. The present invention also provides serial negatively charged protein carriers for preparing the modified antibacterial peptides. The modified antibacterial peptides are applied in medicine, can reduce hemolysis and other side reactions, and may be prepared easily.

The invention provides a wood frog antibiotic peptide and a preparation process thereof. The invention further discloses application of the antibiotic peptide in the preparation of antiviral medicines. The peptide is a basic polypeptide with the molecular weight range from 2 to 16 KDa and the isoelectric point from 8.9 to 9.5. The peptide consists of asparagic acid, glutamic acid, major serine, histidine, arginine, glycine, threonine, proline, alanine, Val, methionine, and the like, wherein the basic amino acid accounts for 23.99 percent of the gross amount, and the acidic amino acid accounts for 19.08 percent of the gross amount. The wood frog antiviral antibiotic peptide is a product obtained from natural resources. The wood frog antiviral antibiotic peptide has the good antiviral capability and simultaneously has the advantages of no hemolytic activity, no plasma coagulation activity, and the like. The wood frog antibiotic peptide can be used for preparing medicines for treating antiviral type flu, hepatitis B and AIDS.

The invention discloses an antibiotic peptide buforin II and porcine INF-alpha fusion expression pichia pastoris (Pichia pastoris FZM2009, CCTCC NO:M209259), and a preparation method and applications thereof. The method comprises the steps: A, the construction of expression cassette PAOX1-alpha Factor-IFN alpha-6xHis-bufforin II-Zeocinr; B the construction of expression engineering bacteria Pichia pastoris FZM 2009, wherein the engineering bacteria is resulted from the screening of antibiotics Zeocin and PCR identification; C, the preparation of fusion protein, which comprises the steps: 1) the recovery is implemented on a YPD plate containing 100mug / ml of Zeocin; 2) single clone is inoculated to a YPD-containing culture medium for vibration and culture; 3) 1 volume of culture is inoculated into 10 volumes of a fermentation culture medium for culture; 4) supernatant is obtained by centrifugation; 5) loading buffer with double volume is added into the supernatant, and electrophoresis detection is performed; 6) the supernatant is subject to a molecular sieve gel column to collect components; 7) the collected components are lyophilized to result in fusion protein; 8) buforin II and IFN alpha are obtained by the enzyme digestion of enterokinase on the fusion protein. The fusion protein buforin II and IFN alpha in fusion expression are applied to pig feeds. The method has great feasibility, simple and convenient operation, high expression, low cost and strong bioactivity.

The invention relates to a bacillus subtilis fmbJ antibacterial peptide fengycin homologue and a preparation method, which belongs to the fields of microorganism fermentation and biological separation. The invention is dedicated to the microorganism fermentation and separation identification of a new antibacterial peptide fengycin homologue. The method of the microorganism fermentation and separation identification of the new antibacterial peptide fengycin homologue includes the fermentation of bacillus subtilis fmbJ, the precipitation separation of antibacterial peptide fengycin homologue acid, methanol extraction and the high-efficiency liquid chromatographic analysis of the antibacterial peptide fengycin homologue, and ESI-MS / CID analyzes and measures the molecular weight of the antibacterial peptide fengycin homologue and carries out a structural identification. The antibacterial peptide fengycin homologue can notably resist eight types of gram-negative bacteria, four types of gram-positive bacteria and six types of moulds.

The invention relates to a genetic-engineering antibacterial peptide, as well as a preparation method and application thereof. The preparation method comprises the following steps: according to the characteristics of the amino acid sequence of Magainin and Cecropin P and LL-37 which are antibacterial peptide, a novel antibacterial peptide gene is designed; the sequence of the gene is synthesized and transformed to be expressed in pichia to form antibacterial-peptide gene transformation pichia engineering bacteria, and to be expressed in a fermentor to achieve the effects of high-density cultivation and efficient expression. Fermentation broth is separated and purified to be an antibacterial peptide product which can be applied to feed and food additives and be used as injection. The antibacterial peptide has the advantages of good antibacterial activity to most gram-negative bacteria and gram-positive bacteria, in particular better effects of inhibiting and killing ampicillin-resistant bacteria and kanamycin-resistant bacteria.

The invention discloses a preparing method of HaiNan speckle equal scorpion antibiotic peptide gene and appliance, which comprises the following steps: restructuring bacillus coli Escherichia coli DH5a / ImAMP1, CCTCC NO: M207034; constructing HaiNan speckle equal scorpion ioterium cell cDNA library; designing primer; choosing PCR method; sieving positive colony of scorpion antibiotic peptide gene from ioterium cDNA library; sequence-analyzing coding trait of antibiotic peptide gene; assuring amino acid sequence of the antibiotic peptide gene; adopting chemosynthesis antibiotic peptide; possessing inhibition of diverse density for Gram's bacterium. This peptide can be used to medicine to prevent and cure Gram's bacterium.

The present invention belongs to the field of biomedicine technology. Wasp antibiotic peptide is a kind of single-stranded polypeptide separated from wasp venom and with molecular weight of 1316.6 dalton, isoelectric point of 8.59, and polypeptide whole sequence of NH2-IDWKGIAAMAKI-COOH. It is prepared through electrically stimulating wasp to collecting wasp venom, centrifuging to eliminate precipitate, freeze drying, chromatographic separation and purification in gel column, ion exchange column and reverse high pressure liquid phase chromatography. The wasp antibiotic peptide has powerful activity of inhibiting bacteria, fungi, viruses and tumor cell growth and the advantages of no hemolysis activity, etc. and may be used in preparing medicine for treating pathogeneic microbial infection disease and tumor.

The invention relates to a method for preparing lactoferrin antibacterial peptide through an enzyme method which belongs to the technical field for preparing the lactoferrin antibacterial peptide. The method of the invention comprises: taking lactoferrin as raw materials, adopting pig pepsin to hydrolyze the lactoferrin under certain condition, and finally obtaining lactoferrin antibacterial peptide mixture with stronger antibacterial activity. The method has simple process and strong practicability, the lactoferrin antibacterial peptide which is obtained has stronger antibacterial property and is broad-spectrum antibacterial peptide, gram-negative bacteria and gram-positive bacteria which contain pathogenic escherichia coli can be inhibited, eukaryotic cells are not affected, and the lactoferrin antibacterial peptide is functional antibacterial peptide and has better application prospect. The method for preparing the lactoferrin antibacterial peptide has important significance for promoting dairy products deep processing and colostrum resources comprehensive utilization, increasing health level of our people especially infants, and researching and developing functional food additives.

The invention discloses an expressing carrier of black porgy antibiotic peptide Hepcidin, expressing product and preparing method, which is characterized by the following: incorporating E. coliTrc promoter, protein project improving CKS gene and procaryotic expressing carrier pTrc-CKS of histidine tag (His-Tag); connecting to black porgy Hepcidin gene; constructing pTrc-CKS / hepcidin expressing plasmid; possessing P3C enzyme cutting site; fusing six histidine on C end; getting CKS-hepcidin; purifying through C end His-Tag affinity chromatography; getting the purified product; cutting CKS with P3C enzyme in fuse protein; getting the purified Hepcidin.

The invention relates to a house-fly pupa natural antibiotic peptide product as well as a preparation method and application thereof; house-fly pupas, the raw material of the antibiotic peptide product, are extracted overnight by acid and hot-processed to collect supernatant; and after going through siever filtration and ultrafiltration precessing, the supernatant is treated with spray dring to obtain the antibiotic peptide product. The preparation technique of the antibiotic peptide product is simple and quick, the yield of the antibiotic peptide reaches 1.2 percent and the recovery rate of the antibacterial activity is over 80 percent. The prepared house-fly antibiotic product consists of peptides which have the molecular weight less than 20kDa and are mainly micromolecular peptides with the molecular weight less than 14.4 kDa. The antibiotic peptide product has broad-spectrum antibiotic activity and no hemolytic activity to blood erythrocytes of mice and is characterized by high temperature resistance, acid and alkali resistance and good stability. The byproducts of the technique can serve as feeding insect protein powder. The prepared antibiotic peptide product can be widely applied to the fields such as the antisepsis and storage of foods, fruits and vegetables, feed additives, cosmetic and the like.

The invention discloses an antibiotic peptide and its preparation and application. According to sequence of natural antibiotic peptide, gene of antibiotic peptide is designed and synthesized in DNA synthesizer by choosing genetic code preferred by yeast. The invention selects dispense of culture medium and optimizes condition of fermentation. The antibiotic peptide can kill many pathogenic microorganisms such as Gram-positive bacteria, Gram-negative bacterium and so on.

The present invention relates to antibiotic preparation for skin, and is especially a kind of antibiotic peptide spray filming preparation and its preparation process. The antibiotic peptide spray filming preparation has antibiotic peptide as the active antibiotic component, and supplementary material comprising water soluble polymer filming material and humectant. The preparation process includes dissolving the water soluble polymer material in hot water, adding the humectant and the antibiotic peptide, mixing and filling in sprayer. After being sprayed to the affected part, the antibiotic peptide spray film preparation forms one film with excellent skin affinity and air permeability in minutes for protecting skin, killing bacteria and preventing infection. The present invention has excellent curative effect on burns and infection.

The present invention relates to a method for expressing antimicrobial peptide CAD by means of a recombinant Bacillus subtilis expression system. The SUMO protease expression operon is first artificially synthesized. The protein expression operon genes of Saccharomyces cerevisiae small ubiquitin-related protein is then fused with the antibacterial peptide AD. The fusion protein is further cloned into the pNF11 plasmid to be introduced into Bacillus subtilis, thereby ensuring the induced expression of recombined Bacillus subtilis in shake flasks. The method has the advantages of a simple expression system, large-scale production, low production cost, strong biological activity and no toxic or harmful substance production. Moreover, the method provides a medicine with low price and strong antibacterial capacity for clinic disease prevention and treatment. This invention can also be used as a feedstuff additive.

The invention discloses a recombinant swine antibacterial peptide PG4, the amino acid sequence of which has amino acid sequence as shown in SEQ ID NO:1. The invention also discloses a method for synthesizing the recombinant swine antibacterial peptide PG4, which includes: (1) constructing antibacterial peptide PG4 prokaryotic expression vector, (2) transforming into Escherichia coli host cell, and constructing expression engineering bacteria; (3) fermenting and culturing the engineering bacteria, and performing inducible expression of inducer; and (4) purifying. The PG4 has the advantages of high expression efficiency, simple separation and purification, convenient operation, easy magnification, good stability, and suitability for large scale industrialized production. The PG4 is of important value for the research of novel high-efficiency antibacterial drug, and has wide application prospect in the fields of pharmacy industry and biological antibacterial material.

A scophthalmus maximus peptide hepcidin gene and its yeast expression vector, the method is to separate and clone Scophthalmus maximus peptide cDNA and ití»s gene groupí»s gene, regroup this geneí»s mature peptides to yeast cell expression vector®ñpGAPZB, then to form confluent expression vector®ñpGAPZB-the6his, and conversion Pichia pastoris strain SMD1168ú¼and get the yeast plant that can express mature peptide albumen steadily, this peptide gene can be considered to finish expression by passing the SDS-PAGE and Western blot test. This invention clone Scophthalmus maximums peptide gene and form mature yeast expression vector in the cell at the first time, it can be used as fish and other aquatic animalí»s feed additive by yeast form directly, and it doní»t request to separate and purificate the regroup peptideíúThe invention also can be used to clone many fishí»s peptidegene and its expression vector, and used to prevention and cure fish and other aquatic animalí»s disease.

The invention discloses Saccharomyces cerevisiae engineering bacteria for expressing recombinant rainbow trout antibiotic peptide Oncorhyncin II and the preparation method thereof. The preparation method comprises the steps as follows: introducing the coding genes of the mature peptide of the rainbow trout antibiotic peptide Oncorhyncin II into the Saccharomyces cerevisiae to obtain the strains capable of secretion-expressing the rainbow trout antibiotic peptide Oncorhyncin II. The Saccharomyces cerevisiae rainbow trout antibiotic peptide Oncorhyncin II is preserved in China General Microbiological Culture Collection Center on June 19th, 2008 with the preservation number of CGMCC No.2554. The Saccharomyces cerevisiae engineering bacteria can stably express the recombinant rainbow trout antibiotic peptide Oncorhyncin II, which has antibacterial activity to gram positive bacteria and gram negative bacteria, such as Escherichia coli, Staphylococcus aureusetc, and can be used for preparing feed additives, fresh-keeping agents, cosmetics and pharmaceutical products.

The invention discloses an antibiotic peptide, blood coagulation apoenzyme slicing sequence and fusion protein sequence of phoroblast growth factor, wherein the antibiotic peptide can be bacteria resistant peptide, antimycotic peptide, or genetic engineering hybrid peptide for bacteria and fungus resistant simultaneously, while the phoroblast growth factor can be acidic phoroblast growth factor (aFGF) or basic fibroblast growth factor (bFGF), the nucleic acid sequence for coding the fusion protein can be inserted into appropriate expression carrier and expressed in Pichia pastoris yeast.

The present invention provides one kind of antibiotic peptide and its coding polynucleotides and use. The antibiotic peptide contains the amino acid sequence from site 1 to site 25 in the sequence table <400>2 of the sequence table. The polynucleotides coding the antibiotic peptide include the nucleotide sequence of amino acid sequence form site 1 to site 25, from site 1 to site 45 or from -22 site to site 45 in the sequence table <400>2 of the sequence table or their complementary sequences. The antibiotic peptide has obvious killing effect on several kinds of bacteria, and has lowest killing concentration smaller than 10 micro mol / L to algae dissolving vibrio, vibrio vulnificus, parahemolytic vibrio, Pasteuvella multocida, etc. and may be used in preparing antibiotic, especially that for preventing and treating bacterial diseases of aquatics.

The invention provides an identifying protein gene for a silkworm peptidoglycan. First of all, predicating PGRPs gene by using a silkworm gene database and EST database source, then extracting PGA-induced 5-instar silkworm epiploon, synthesizing CDNA, and cloning gene. PGRPs gene can obviously kill bacteria, and PGRPs pertain solution of low concentration can also suppress bacteria growth. The congenital immunity identifying receptor of silkworm activates the congenital immunity and adjusts the antibiotic peptide expression, and can be used for culturing antibiotic silkworms.

This invention relates to modified antibiotic peptides, particularly for use in medicine. The invention further relates to compositions and methods for destroying microorganisms, such as bacteria, viruses or fungi, and to methods for treating microbial infections. The object of the invention is to develop novel antibiotic peptides, particularly having enhanced antibiotic activity and an expanded spectrum of activity against other strains of bacteria, particularly gram-positive bacteria such as Staphylococcus aureus. According to the invention, the object is attained in a first aspect by a peptide according to claim 1.

The invention relates to a method of isolating antibacterial peptides and the isolated peptides thereof. The method comprises: constructing a fusion DNA sequence of yeast alpha mating factor leader sequence and a random peptide, inserting the sequence into a saccharomyces cerevisiae vector which contains an inducible promotor, digesting the unlinked linear DNA with exonuclease III and mungbean sprout nuclease, electrotransforming the yeast vector after purification, culturing the transformant 24h and covering the transformant with the upper medium which contains E. coli K12D31, and detecting the bacterial inhibition ring and the bacterial inhibition rate after inducing the expression by liquid medium, selecting the positive clones and amplifying by PCR, and determining the sequence. The method of the present invention can be used easily and effectively to isolate antibacterial oligopeptides and polypeptides and can be used as an alternative way to find antibiotics.

The invention relates to a natural antibiotic peptide HKPLP derived from hippocampus and a gene hkplp for coding the protein. The DNA sequence of the natural antibiotic peptide HKPLP is represented by the sequence No.1 in a sequence table, and the protein coded by the gene (HKPLP) is represented by the sequence No.2 in the sequence table. In the invention, a new hkplp antibiotic peptide gene is designed according to the preferred codons of the pichia pastoris, the DNA sequence of the gene is synthesized and transferred to the pichia pastoris KM71 to be expressed, and thus, the pichia pastoris engineering strain of the antibiotic peptide is formed. Meanwhile, the fermentation conditions of the strain are optimized to realize high-density fermentation and high-efficiency expression. The fermentation liquor of the engineering strain has obvious inhibition effects on Gram positive bacteria, Gram negative bacterial, fungi and the like. The processed product of the fermentation liquor was added into feed for shrimp, chicken and the like, and good experimental effects were obtained. The antibiotic peptide product can replace antibiotic, prevent diseases of the digestive tract, promote growth, increase weight and used as an additive for livestock and aquatic product feed.

The invention discloses the method for purifying and preparing recombinant antibacterial peptide of Xinjiang silkworm, recombinant antibacterial peptide of Xinjiang silkworm, application of recombinant antibacterial peptide of Xinjiang silkworm in bacteriostasis and antitumor. recombinant antibacterial peptide of Xinjiang silkworm is obtained from Xinjiang silkworm through cloning, expressing in eukaryotic expression system. The preparing method is: centrifuging the fermentation broth to remove the precipitant, freeze-drying, separating and purifying through an ion exchange chromatography, a hydrophobic interaction chro-matography, and a gel filtration chromatography to obtain the final product. The recombinant antibacterial peptide of Xinjiang silkworm has high inhibition and killing activity on bacteria and tumor cells, no hemolysis activity, and no toxicity to normal tissue cells, and can be used for preparing drugs for treating infectious diseases caused by pathogenic microorganism and tumors.