85 results about "Pepsin inhibitor" patented technology

The invention relates to a method for preparing a fish oil ethyl ester microcapsule from fish pomace, which mainly comprises the extraction of fish oil, the preparation of fish fatty acid ethyl ester, the purification of fish oil ethyl ester and the preparation of a fish oil ethyl ester microcapsule, wherein the extraction of fish oil comprising the following steps: eliminating foreign matters from fish viscus, cleaning, mashing, adding pepsin and neutral protease for enzymolysis, then adding anhydrous alcohol, standing to stratify, and centrifuging to obtain the fish oil; the preparation of fish fatty acid ethyl ester comprising the following steps: mixing the fish oil with ethanol, making the mixture to be subject to an esterification reaction under the catalysis of sodium ethylate, vaporizing the ethanol, washing with water, and then centrifuging to obtain the fish oil ethyl ester; in the purification process, supercritical CO2 extraction is adopted, the purified fish oil ethyl ester is a golden yellow transparent liquid, and EPA ethyl ester and DHA ethyl ester contents thereof are more than 50%; and the preparation of a fish oil ethyl ester microcapsule comprises the following steps: mixing sodium alginate, maltodextrin and sodium caseinate, dissolving in water, adding the purified fish oil ethyl ester, and adopting a spray drying method to obtain the powdered fish oil ethyl ester microcapsule which can hide the fishy smell of the fish oil ethyl ester, improve the mouth feel and be beneficial to packaging and storage.

The invention relates to a method for preparing fish scale collagen. The method comprises the following steps: fresh fish scales are taken as a material, undergo cleaning, drying and crushing, are soaked in NaOH solution for degreasing, and soaked in hydrochloric acid solution for deashing; the fresh fish scale is added with water of which the weight is 5 to 10 times of that of the fish scales, the pH value is adjusted to between 2 and 4 by acid solution, pepsin of which the weight accounting for the weight of the mixing solution is between 1 and 5 percent is added, the mixing solution undergoes enzymolysis at a low temperature for 4 to 10 hours, and enzyme is killed; enzymatic liquid is filtered twice to obtain extracting solutions, the extracting solutions are mixed, the mixing solution is decolored, sodium chloride of which the weight accounting for the weight of the mixing solution is between 5 and 15 percent is added into the mixing solution for overnight salting out, deposit is separated and collected, and acetic acid of which the weight accounting for the weight of the mixing solution is between 5 and 20 percent is added into the deposit for dissolution to obtain protein solution; the protein solution is dialyzed and desalted to obtain purified collagen liquid; and the collagen liquid is subjected to freeze-drying or spray-drying and micro-crushing to obtain solid particle. The method has the advantages of reasonable process, simple operation, safety, low cost, little loss of nutrient matters of the product, low ash content and high yield and purity of the collagen.

This invention discloses a method for preparing freshwater fish skin collagen. The method comprises: (1) washing fish skin, draining, cutting, and soaking in 0.001-0.2 M alkali solution containing 0.005-2% (v / v) H2O2 for 3-48 h; (2) stirring at a high speed, washing and soaking with an organic reagent; (3) extracting with acid solution (pH = 1.0-5.0) containing 0.01-2% (m / v) pepsin for 12-64 h, and filtering with 30-100 mu.m membrane, and then with 1-30 mu.m membrane; (4) adding 5 M NaCl solution into the filtrate to 0.8-2.0 M, centrifuging, dissolving the precipitate in 0.001-0.1 M acetic acid, dialyzing with distilled water, and freeze-drying to obtain white and odorless collagen solid. The collagen can be widely used in food, cosmetic and biomedical fields.

The invention discloses a method for extracting an anti-gastric cancer active component from water chestnut shells. The method sequentially includes the following steps that: (1) water chestnut shell powder is added with buffer solution with a pH value being equal to 2 and pepsin, kept still for extraction under 37DEG C of water bath and centrifugated; (2) filter residue obtained in step 1 is added with buffer solution with a pH value being equal to 8 and trypsin, kept still for extraction under 37DEG C of water bath and centrifugated; and (3) supernate obtained in step 2 is centrifugated at 12000 to 14000 rotations per minute for 25 to 35min, and after centrifugation, the obtained liquid is lyophilized, so that the anti-gastric cancer active component is obtained. The anti-gastric cancer active component extracted by adopting the method is characterized by high activity.

The invention provides a method for preparing radix astragali homopolysaccharide in the technical field of Chinese medicine. The method comprises the following steps that: radix astragali coarse powder is taken, refluxed and degreased, filtered, refluxed and extracted with water, concentrated, precipitated, centrifuged or filtered to collect precipitate, and the precipitate is washed and dried in vacuum to obtain radix astragali coarse polysaccharide; aqueous solution of the radix astragali coarse polysaccharide is enzymatically hydrolyzed with pepsin, boiled and centrifuged to obtain supernatant fluid; a Sevage agent is added to the supernatant fluid for shaking and extraction so as to collect upper layer aqueous phase solution; diethylaminoethyl cellulose is added to be filtered, concentrated, dialyzed, precipitated, centrifuged or filtered, washed and dried to obtain the refined radix astragali homopolysaccharide; the refined radix astragali homopolysaccharide is separated and purified through column chromatography twice, and eluted with water, the eluant is collected, concentrated, refrigerated and dried to obtain the radix astragali homopolysaccharide. The average molecular weight of the radix astragali polysaccharide obtained in the invention is about 9000 and the radix astragali homopolysaccharide only contains glucose monosaccharide unit after a complete acid hydrolysis.

Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion / trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the i0nvasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.

The invention provides a preparation method of cartilage extract containing non-denatured type II collagen. The method comprises the following steps: degreasing, disinfecting, homogenizing, enzymatic hydrolysis, filtration and drying. In the enzymatic hydrolysis step, the pH of a slurry obtained in the homogenizing step is adjusted to 2.5-8.5, an enzyme accounting for 0.001-2%of the weight of cartilages is added, a clear liquid accounting for 1 / 20 to 1 / 500 of the weight of the cartilages and obtained through juicing pawpaw and / or pineapples and filtering the obtained juice is added, the enzymatic hydrolysis is carried out at 25-50 DEG C for 12-48 h, and the enzyme is preferably selected from one or more of pepsin, subtilisin, alkaline proteases and metalloproteases. The cartilage extract obtained through the preparation method has the advantages of high purity, high extraction rate, and easiness in large-scale production, and richness in non-denatured type II collagen and chondroitin sulfate.

The invention relates to a soaking technology for preparing corn starch, belonging to the technical field of grain deep processing. The technological steps comprise: 1. soaking: corn grains are soaked in water to soften embryos; 2. pulp grinding: the soaked corn grains are coarsely ground to obtain corn pulp; 3. enzymolysis: one of acid protease and pepsin is added in the obtained corn pulp after coarse grinding to conduct enzymolysis to the corn pulp, and SO2 is fed at the same time to mainly take the effect of inhibiting activities of microorganisms on the premise that the enzymatic activity is not affected; and 4. fine grinding, separation and drying to obtain starch finished product. Through shortening soaking time and reducing the used amount of SO2, the invention has the advantages that the corrosion of equipment, the underground water pollution, the residual sulfurous acid in the product and the like are obviously reduced, the obstacles restricting enzyme to enter endosperm to react with protein particles are eliminated, the total soaking time is only 8-12h, the soaking time is shortened, the cost is reduced, the effect is good, and the popularization value and the application value are high.

The extraction process of comb shell polysaccharide includes the technological steps of material treatment, homogenizing, ultrasonic treatment, enzymolysis, separating, concentration, alcohol precipitation and drying. In the material treatment, the comb shell meat tissue including leftover is taken and may be stoved or freeze dried; and in the enzymolysis, one or two of trypsin, pepsin and neutral subtilisin are used. The present invention provides the technological path of extracting comb shell polysaccharide and the comb shell polysaccharide product has high purity and high yield, and may be used in developing various functional foods.

This invention provides botulinum antitoxin compositions and methods of production, and methods of treating animals and humans prophylactically and also those suspected of having contacted botulism toxin. The botulinum antitoxin is prepared by inoculating an animal with a monovalent botulinum toxoid and toxin. The animal's plasma is collected and purified at a high pH by affinity chromatography. The resulting monovalent immunoglobulins are de-speciated by digestion with pepsin. Monovalent antitoxins for all seven botulinum serotypes are then combined to produce a high titered heptavalent botulinum antitoxin composition.

The invention relates to a preparation method of a tuna blood pressure lowering peptide. The preparation method comprises steps of homogenizing tuna tissue, adding pepsin or papain of 1,000-13,000 U / g fish pulp at the pH value of 2-5, heating to 35-50 DEG C, performing enzymolysis in a water bath for 1-4 hr, heating hydrolysate with boiled water for 5-15 min for deactivating enzyme, cooling, centrifuging, and purifying with macroporous resin or dextrane gel resin. The preparation method is scientific and reasonable, and extracts blood pressure lowering peptide with the leftovers of natural tuna as raw material. The blood pressure lowering peptide prepared by hydrolysis has good effect, easily-accessible raw material and low cost, and can be used for developing health products or medical preparations. The extraction method is simple, economic and easy for operation, and can be carried out in a common biochemical laboratory.

It is intended to provide a skin collagen production promoter, foods and drinks for promoting skin collagen production and cosmetics for promoting skin collagen production which are useful in preventing skin chapping, wrinkles, worsening in skin fitness, etc. Namely, a skin collagen production promoter, foods and drinks for promoting skin collagen production and cosmetics for promoting skin collagen production which contain as the active ingredient(s) a milk-origin basic protein fraction and / or a basic peptide fraction obtained by digesting the above-described basic protein fraction with a protein digesting enzyme such as pepsin or pancreatin. The above basic protein fraction and basic peptide fraction have an effect of increasing skin collagen level.

Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion / trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the i0nvasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.

The invention relates to a method for extracting a collagen from fish scales. The method comprises the following steps: carrying out pretreatment, drying, degreasing, deliming, irradiation treatment, enzymolysis, extraction, purification and the like on the fish scales taken as raw materials, thereby extracting the collagen from the fish scales. The enzymolysis, the extraction and the purification of the fish scales taken as the raw materials are carried out by the combination of pepsins with citric acids after the fish scales are treated under the irradiation of electron beams, so that the enzymolysis time is shortened; the hydrolysis degree is improved; the production efficiency is improved. The method has the advantages of high extraction rate, small relative molecular weight, high collagen purity, low preparation cost and secondary pollution avoidance.

The invention relates to a method for preparing lactoferrin antibacterial peptide through an enzyme method which belongs to the technical field for preparing the lactoferrin antibacterial peptide. The method of the invention comprises: taking lactoferrin as raw materials, adopting pig pepsin to hydrolyze the lactoferrin under certain condition, and finally obtaining lactoferrin antibacterial peptide mixture with stronger antibacterial activity. The method has simple process and strong practicability, the lactoferrin antibacterial peptide which is obtained has stronger antibacterial property and is broad-spectrum antibacterial peptide, gram-negative bacteria and gram-positive bacteria which contain pathogenic escherichia coli can be inhibited, eukaryotic cells are not affected, and the lactoferrin antibacterial peptide is functional antibacterial peptide and has better application prospect. The method for preparing the lactoferrin antibacterial peptide has important significance for promoting dairy products deep processing and colostrum resources comprehensive utilization, increasing health level of our people especially infants, and researching and developing functional food additives.

The invention relates to the field of biotechnology, and especially relates to a preparation method of an animal placenta extract. According to the invention, animal placentas are pretreated, and are respectively mixed with pepsin and trypsin for enzymolysis. Extraction is carried out with an enzymolysis method, such that product purity and quality are greatly improved. With other direct hydrolysis methods, with the method provided by the invention, the contents of obtained placenta powder polypeptide and free amino acids are high, and fat content is very low. During an impurity-removing process, besides ultrafiltration, agarose gel hydrogen bond adsorption chromatography is adopted, such that high-molecular impurities are removed, and product purity is further improved.

The invention relates to a method for preparing angiotensin I-converting enzyme (ACE) inhibitory peptide by using a squid liver protein, which comprises: treating squid liver protein from which fish oil is extracted with acetone to degrease; hydrolyzing the squid liver protein in the presence of pepsase for 18 to 25 hours under conditions that: the hydrolysis temperature is 35 to 38 DEG C, the pH is 1.5 to 2.5, the ratio of the pepsase to a substrate is 2 to 3 percent, and the concentration of the substrate is 1 to 3 percent; and subjecting enzymolysis solution to ultrafiltration treatment, tracking the ACE inhibiting activity of the ACE inhibitory peptide, and separating and purifying the ACE inhibitory peptide by using gel filtration chromatography, diethylaminoethanol (DEAE) anion exchange, reversed-phase-high performance liquid chromatography (RP-HPLC) and other methods. The ACE inhibiting activity of the prepared ACE inhibitory peptide is basically unchanged at a temperature of 2 to 100 DEG C and a pH value of 1 to 12 and is stable; and in a simulation gastrointestinal digestion experiment, the ACE inhibiting activity of the components is not affected, and the peptide still has high ACE inhibiting activity after being digested in gastrointestinal tract. The method is reasonable in process, simple in operation, reduces waste discharge, changes waste materials into valuable materials, and the prepared ACE inhibitory peptide can be used in the field of development of blood pressure reducing foods and medicines.

The invention discloses a method for producing small peptides through bionic enzymatic hydrolysis. The method is characterized in that the crushing and sieving mesh, the addition amount, the enzymatic hydrolysis temperature, the pH value and the enzyme addition amount are controlled and optimized; the addition amounts of pepsin and trypsin are small, so the cost is saved; and the yield of bionic enzymatic hydrolysis is high, and pepsin and trypsin combination is used to carry out bionic enzymatic hydrolysis in order to make the paste yield be 1.5-2 times that of traditional technologies. An ultrafilter membrane separation + electrodialysis + resin column chromatography combination technology is used to carry out separation and purification in the bionic enzymatic hydrolysis technology, the loss amount of active peptides is smaller than 2%, and the content of micro-molecular peptides with the relative molecular mass being smaller than 2KD is greater than 80%. The method has the advantages of reduction of the loss of the active peptides obtained through enzymatic hydrolysis, high content of the micro-molecular peptides, and improvement of use values.

The present invention relates to the technical field of Traditional Chinese medicine, in particular to a traditional Chinese medicine composition and the use thereof. The traditional Chinese medicine composition comprises the following ingredients: honeysuckle, tangerine peel, stir-baked malt, honey, and hawthorn juice. The traditional Chinese medicine composition of the present invention has significant promotions on the gastric emptying rate, intestinal motility, gastric digestive enzyme (pepsin) activity and pancreatic enzyme (trypsin, chymotrypsin, amylase and lipase) activity of rats, exhibiting that the traditional Chinese medicine composition of the present invention has significant effects of clearing heat-fire and moistening the intestine.

Disclosed is a highly safe and effective therapeutic agent for eating disorders, which contains, as an active ingredient, a water-soluble fraction of a hydrolysis product of casein. The hydrolysis product is obtained by means of pepsin.

The invention discloses a composite oligopeptide with high oxidation activity inhibition and a preparation method thereof. The preparation method of the composite oligopeptide with high oxidation activity inhibition comprises the following steps: (1) respectively hydrolyzing egg white protein and soybean protein by using a single enzyme or double enzymes of pepsin and alkaline protease, and obtaining one of the egg white protein hydrolysate and the soybean protein hydrolysate; (2) enriching high-hydrophobicity antioxidative peptide in the two protein hydrolysate sources by using resin in an ultra-filtration manner, and obtaining a target peptide, namely a rough resin separation component, by virtue of gradient elution; and (3) respectively compounding every two of the protein hydrolysate sources obtained in the step (1) or / and two protein hydrolysate sources obtained in the step (2), thereby obtaining the composite oligopeptide. The composite oligopeptide disclosed by the invention is high in oxidation activity inhibition, and the preparation method is simple.

The present invention relates to phytase enzyme, a gene encoding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and the Citrobacter braakii producing the enzyme. The phytase enzyme and the Citrobacter braakii producing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.

The invention relates to a method for determining composition of a disulfide bond of a recombinant human granulocyte colony stimulating factor, which belongs to the technical field of proteomics. Themethod combines a LC-Q-Q-TOF combination technology and a post-column on-line reduction method for determining an rhG-CSF disulfide bond and a disulfide bond mismatching proportion. The test step comprises the following steps: performing enzyme digestion on rhG-CSF under condition of pH 5.0 of a buffer solution by pepsin, performing LC-MS detection of a reduced sample and an unreduced sample, andperforming on-line post-column on-line reduction liquid chromatogram-mass spectrometry detection on the unreduced sample. The method can perform direct enzyme digestion on rhG-CSF under acidic condition, and omits denaturation and alkylation steps, so that operation is concise, the reaction condition is mild, repeatability is good, and the sensitivity is high; the post-column on-line reduction method is matched to obviously reduce the data analysis difficulty, the identification of the disulfide bond of rhG-CSF and calculating of the disulfide bond mismatching proportion result are more reliable, the experiment process is concise, and the data analysis is efficient.

The invention relates to a promega for testing glycolated hemoglobin by using an enzymatic method, which contains the ingredients of hemolysis buffer: 10-1000 mmol / L of N-chclohexyl-2 ethyl amine sulfoacid, 10-1000mmol / L of morpholino propane sulfoacid, and 1-100g / L of polyethylene glycol oxide dodecyl ether; reagent R1a: 10-1000kU / L of pepsin of pig, 0.1-10mmol / L of 2-(iodophenyl)-3-(2, 4-dinitro benzene)-5-(2, 4-sulfophenyl)-2H tetrazole sodium salt, and 0.0001-0.1mol / L of HCl; reagent R1b: 0.1-10mmol / L of morpholino ethane sulfonic acid, and 0.5-50mmol / L of CaCl2; neutralization buffer: 0.0001-0.1mol / L of NaOH reagent, 2:1-1000kU / L of fructose valine oxidase, 30-600kU / L of peroxydase, 0.01-0.5mmol / L of N-(carboxymethyl aminocarbonyl)-4,4 quadri (dimethyl amine)-diphenylamine sodium salt, and 10-1000mmol / L of Tris-HCl. The promega has the zymohydrolysis effect which is better than alkalescence prolease, little dosage, low cost and accurate measuring result.

An apparatus for detecting pepsin comprising a solid support, and a peptide chain wherein the peptide chain is operatively configured to be cleaved by pepsin, and the peptide chain is disposed on a surface of the solid support.

The invention discloses a method for impinging stream assisted extraction of collagen. The method mainly comprises the following steps: (1) treatment of fresh cattle achilles tendon with alkali; (2) accelerated extraction by adopting an impinging stream assisted method in the process of enzyme extraction of the collagen; (3) precipitation and separation of the collagen in an extract; and (4) washing and freeze-drying of the collagen. According to the invention, the collagen is extracted in an acidic aqueous solution under the actions of pepsin and impinging stream assisted external force, and a stable triple-helical structure of a collagen molecule is accelerated to be unwound into a single-helical structure, so the process of extracting the collagen is accelerated; and the technical process is simple and easy, improves the extraction rate and the purity of the collagen, shortens the production period, and maintains the biological activity of the collagen.

The invention provides an antihypertensive active rapeseed peptide and a preparation method and application thereof. The preparation method of the antihypertensive active rapeseed peptide includes obtaining crude extract of the antihypertensive active rapeseed peptide by pepsin and pancreatic enzymatic hydrolyzed rapeseed protein, and obtaining the antihypertensive active rapeseed peptide by refining. The invention further provides the antihypertensive active rapeseed peptide prepared by the method and application thereof. Sources of rapeseed meal for preparing the antihypertensive active rapeseed peptide are extensive, production process is simple, low in cost and environment-friendly, and the method is suitable for industrialization. The antihypertensive active rapeseed peptide causes low gastrointestinal reaction, is capable of resisting hydrolysis of human digestive enzymes, and has good antihypertensive effect by actively reacting to target organs. The antihypertensive active rapeseed peptide is capable of inhibiting not only ACE (angiotensin converting enzyme) but also renin activity, is capable of avoiding alternative pathways of a RAS (renin angiotensin system) activated by taking antihypertensive drug, ACE inhibitor, for long, and has bright application prospect in terms of lowering blood pressure.

The invention discloses a method for preparing wood frog skin collagen, which comprises the following steps: obtaining wood frog skin powder; pretreating; adding into an acetic acid solution, irradiating under microwaves, stirring, and centrifuging to obtain a precipitate and a solution containing acid-soluble collagen; dispersing the precipitate in a pepsin-containing acetic acid solution, centrifuging to obtain a supernate, dialyzing the supernate, centrifuging to obtain a precipitate, and dissolving the precipitate in an acetic acid solution to obtain a solution containing enzyme-soluble collagen; adding NaCl into the solution containing acid-soluble collagen and the solution containing enzyme-soluble collagen, stirring, standing, and adding NaCl and trihydroxymethyl aminomethane to obtain a solution; regulating the pH value of the solution to obtain a precipitate, dissolving the precipitate in an acetic acid solution to obtain a solution, and dialyzing the solution until the pH value is neutral, thereby obtaining the solution; and prefreezing the solution and drying to obtain the wood frog skin collagen. The method can enhance the collagen extraction rate.

The invention discloses a method for extracting collagen from animal sebum. The method is based on an acid method for extracting collagen, is improved on the basis of the acid method, uses glacial acetic acid as a carrier to be combined with pepsin as a catalyst so as to avoid problems of generation of odor during use of citric acid and low extraction efficiency of acetic acid, and uses a method of dialysis for separation and purification so as to greatly improve the purity of an obtained collagen solution and achieve better quality.

The invention discloses a method for evaluating the antioxidant activity of fruit and vegetable food and functional health product. The method comprises the following steps: preprocessing a sample to be detected: making a raw material to be detected into water homogenate or directly taking the oral liquid type health food as the sample; co-processing and extracting the sample by multiple enzymes in sequence: processing the sample by pepsin, processing the sample by trypsin, processing the sample by pronase E, and processing the sample by plant composite hydrolase Viscozyme L; removing the reducing sugar in the extract through a cold ethyl acetate extraction technology; and finally analyzing the antioxidant substance content and activity by a conventional method. During the extraction process of substances with antioxidant activity, multiple enzymes are used to carry out enzymatic hydrolysis so as to simulate the human digestion process, at the same time, a cell level antioxidant activity analysis method is used, and thus an antioxidant activity evaluation method, which has stronger biological relevance and is more close to animal experiment level, is established.